TY - BOOK AU - Qamar Raza Qadri (2009-VA-569) AU - Dr. Tahir Yaqoob AU - Dr. Abu Saeed Hashmi ED - TI - Polymorphism Of The Slc11a1 Gene Associated With Resistance To Bovine Tuberculosis U1 - 2332-T PY - 2015/// KW - Department of Molecular Biology and Biotechnology N1 - Bovine Tuberculosis (bTB), caused by Mycobacterium Tuberculosis is a health threat to livestock. Information on genetic resistance or susceptibility because of polymorphisms of candidate genes could be used in making selection decisions. Solute carrier family 11 (protoncoupled divalent metal ion transporters), member 1 gene (SLC11A1), is a known candidate gene which is associated with natural resistance to infection by Mycobaterium spp in buffalo. Polymorphism in this gene can be studied for breeding disease resistance animals. Blood samples were collected from Nili Ravi buffalo breed of Pakistan. DNA was extracted by organic method. Primers were designed using Primer3 software. Amplification of gene was done by Polymerase Chain Reaction. PCR products were sequenced bi-directionally on ABI 3130XL Genetic analyzer. Sequence alignment was performed for polymorphism identification. The analysis of identified polymorphism has been done by CHROMAS software. Sequences were aligned by BLAST tool of NCBI. The results of analysis showed that no polymorphisms were identified in exonic region of gene. This might be due to less sample size. Genetics play important role in fighting against pathogens. Identifying the genes involved can lead to marker-assisted selection strategies ER -