TY - BOOK AU - Fatima Sajjad (2010-VA-312) AU - Prof. Dr. Masood Rabbani AU - Dr. Sehrish Firyal ED - TI - Evaluation Of Inhibition Activity Of Indigenous Lactobacilli Spp. On Ammonia Emitting Bacteria U1 - 2594-T PY - 2016/// KW - Microbiology N1 - Poultry is the 2nd largest industry in Pakistan which is growing at an amazing rate of more than 10% from last few years. It provides jobs for more than 1.5 Million people. Although, it is growing at excellent pace, it still faces many problems. One of the important problems in poultry farming is the production of ammonia by urease producing microbes. Ammonia is health hazard for both poultry and human. Urease producing bacteria which are the major problems in poultry are Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae and Staphylococcus aureus in poultry droppings. Probiotics such as Lactobacilli can inhibit the urease producing bacteria in poultry intestine as well as environment thus mitigate the ammonia emission. Present study was designed to isolate Lactobacilli from indigenous poultry, screen them for antimicrobial activity against urease producing microbes and determine their effect on growth of Proteus mirabilis during co-culture experiments. A total of 71 Lactobacilli isolated were recovered from 20 samples of droppings (10) and caeca (10) of back-yard poultry on MRS agar plates. Twenty seven (27) isolates demonstrated antimicrobial activity against ammonia emitting bacteria by agar spot and well diffusion assays. Seven isolates (FSL19, FSL25, FSL39, FSL45, FSL51, FSL63 and FSL71), having better antimicrobial activity, were selected for co-culturing with Proteus mirabilis in nutrient broth. FSL25, FSL45, and FSL51 showed more than 2 log10 reduction of Proteus mirabilis in co-culture experiments. FSL25, FSL45, and FSL51 were identified as Lactobacillus plantarum, Lactobacillus fermentum and Lactobacillus salivarius, respectively by amplifying and sequencing their partial 16S rDNA. It is concluded that Lactobacillus plantarum FSL25, Lactobacillus fermentum FSL45 and Lactobacillus salivarius FSL51 may be used to mitigate ammonia emitting bacteria in poultry environment after further investigations ER -