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Isolation And Identification Of A Protease Producing Bacteria From The Decaying Plant Material And Investigation Of Its Potential As A Probiotic In Broiler

by Abdul Razzaq (2014-VA-498) | Mr. Shahid Abbas | Dr. Rahat Naseer | Dr.Muhammad Imran.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Biochemistry is gaining ground quickly due to the different preferences that it offers over customary chemical procedures particularly in regards to environment and cost contribution. Now a day’s industrial enzymes speak to the heart of biochemistry forms. Proteases are groups of organic catalysts. They have catalytic function.They hydrolyze peptide obligations of proteins and to separate them into polypeptides or free amino acids. Proteases are universal in nature and found in a wide range of differing qualities of sources, for example, plants, animals and microorganisms. Proteases do different organic procedures. Diverse types of proteases are available, alkaline proteases are a standout amongst the most imperative enzymes. The cost of the strain is a special in enzyme production and their applications in different industrial processes. The imaginative part of the present work was to distinguish and detach alkaline proteases from different organic sources, for example, from soil microorganisms with a reason to have beneficial outcome for hydrolyzing the proteins. The purified strain was checked for their potential part in chicken as a probiotics. Protease producing bacteria act as probiotic to improve digestion which results in weight gain in broiler. In this research project, bacterial strains were isolated from decaying organic plants and soil samples collected from decaying organic plants of UVAS Lahore, Pakistan. Soil and decaying samples (1.0 g) were mixed in 100 ml normal saline, afterward serially diluted from 10−1 to 10−6 ratio with normal saline. 100 μl of each diluted sample inoculated in cultural medium and incubated at 37 °C for 48 h. The isolated colonies were selected to obtain pure bacterial cultures. Cultures were maintained in nutrient agar slants. Bacilli were isolated and screened from soil samples for production of proteases. The strains were identified morphologically, biochemically and on their molecular basis. The enzyme (protease) activity was assayed according to standard procedures by slight modifications. The strain was grown and fed to chicken as probiotic. The effects of probiotic were tested for growth of chicken. The probiotics improved the intestinal microbial balance and helped broiler in gaining weight. Bacterial stain studied in the current project may be used on commercial scale for promoting or enhancing the growth of broiler chicken. Availability: Items available for loan: UVAS Library [Call number: 2717-T] (1).

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A Study Of Chicken Gut To Determine The Colonization Of Bacillus Licheniformis And Changes In The Histological Features

by Arooj Tahir (2015-VA-812) | Mr. Shahid Abbas | Dr. Muhammad Imran | Dr. Saif-ur-Rehman Kashif.

Material type: book Book; Literary form: not fiction Publisher: 2017Dissertation note: The most important and commonly used source of animal protein in the whole world these days is poultry meat. With the increase in the world population the demand of poultry meat is also increasing. Universally, for the treatment of infected chicken and for growth promotion, antibiotic growth promoters are used. But the extensive and long term use of AGPs for growth promotion and infection treatments has resulted in the survival of resistant bacterial strains that poses a very drastic threat to both animal and human health. Because of these radical results, some of the countries have restricted the use of AGP in poultry and are moved on towards the use of probitics as growth promoters. The most commonly used strain for probiotics is from genus Bacillus as they have the tendency to survive even in the harsh and industrial conditions. Bacillus subtilis, Bacillus licheniformis and Bacillus pumilusare are the ones that are involved in the spoilage of food and certain medical conditions. In this study, we considered the effects of probiotics as an alternative of AGP in poultry. Bacillus spp. can be used as probiotics, as they can efficiently colonize in the small intestine and improve the growth enhancement in broilers. A selective strain of bacteria Bacillus licheniformis was used as a probiotic for the broiler chicken. After 42 days, 15 birds from three different groups were collected, 5 from each group, and slaughtered to collect cecum samples and intestinal tissue samples. The samples were processed for DNA extraction, PCR and histological methods to determine the probiotics colonization and growth differences between the normal ones and the treated ones. There was a considerable increase in the height of the villi in the treated ones as compared to the control ones which showed that the use probiotic helped increase the surface area of the intestine for increased absorption. The extracted DNA from the cecum sample was used for PCR amplification and sequencing, the results confirmed the presence of Summary 64 Bacillus licheniformis. The results showed that the probiotic was efficiently colonized in chicken gut and it improved the gut health and also helped chicken in gaining weight. Availability: Items available for loan: UVAS Library [Call number: 2893-T] (1).

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