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1. Role Of Single Injection Of Prostaglandin F2 Alpha On Breeding Efficiency Of Buffaloes

by Sajid Iqbal | Prof. Dr. Muhammad Aleem Bhatti | Dr.Imtiaz Hussain Khan | Dr.Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Nature of contents: biography; Literary form: Publisher: 1998Dissertation note: In the present study, a total of twenty Nili-Ravi buffaloes were divided into two equal groups. In group A ten buffaloes were administered with prostagladin F2 alpha (Lutalyse, Upjohn), 2 hours after calving. In group B, ten buffaloes were not given any treatment and designated as control. The reproductive organs of each experimental buffalo were rectally palpated on day 14 and day 21 postpartum. After that twice a week rectal palpation was carried out until the first postpartum oestrus. The results of present study revealed that cervical and uterine involution was completed significantly (P < 0.05) earlier in group A as compared to group B (28.90± 1.79 and 35.40±3.95 days). There was no significant difference in the diameter of cervix, gravid and nongravid uterine horn at day 14 postpartum. A significant difference between the groups was obtained on days 21, 25 and 28 postpartum in the diameter of cervix and gravid horn. The corpus luteum (CL) of pregnancy regressed very rapidly following calving. The overall period required for complete regression of corpus luteum of pregnancy was (19.20±4.87 days) in treated group and (18.40±6.07 days) in control groups. The difference was significant. Follicular activity resumed independently of uterine involution. It was, however, delayed slightly by the retained corpus luteum of pregnancy. The mean postpartum interval of initial follicular development was 21.20±5.71 days in treated and 28.20±8.75 days in control groups, respectively. The difference was statistically significant (P <0.05). Postpartum oestrus interval was shortened in treated group (79.50±19.83 days) as compared to control group (103.0± 17.45 days) and the difference was significant (P<0.05). So it seems beneficial to administer prostaglandin F2 alpha in postpartum buffaloes to reduce the period for uterine involution and enhance the subsequent reproductive performance. Availability: Items available for loan: UVAS Library [Call number: 0585,T] (1).

2. A Study On The Effect Of Gnrh Analogue On Anovulation In Repeat Breeding Cross Bred Cows And Their Conception Rate

by Asif Rafiq | Prof.Dr.Rashid Ahmed Ch | Dr.Imtiaz Hussain Khan | Dr.Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1999Dissertation note: The incidence of repeat breeding is high in cross-bred cows due to low LH level in the blood at the time of estrous. In this study Dalmarelin a GnRH analogue which induces ovulation in cows was used. Nine healthy animals with the history of repeat breeding and clinically normal genital tract were selected at the Livestock Production and Research Institute, Qadirabad, Okara. These animals were injected with dalmarelin (Lecirelin), at the dose rate of 2 ml IM. The injection was given on the zero day of estrous and later animals were inseminated artificially. Two blood samples were collected from these animals, one before the injection and artificial insemination i.e. on the zero day of estrous and the second at 10th day after the injection of Lacirelin for the detection of plasma progesterone concentration. Results indicated that five animals i.e. 55.5% had conceived. Similarly when detected 12 hours after the end of estrous five animals had ovulated out of which three animals were conceived. Finally, these animals were palpated rectally on the 90th day after artificial insemination and five animals with high concentration of progesterone on the 10th day, were, detected positive for pregnancy. None of the control animals ovulated and conceived. Availability: Items available for loan: UVAS Library [Call number: 0623,T] (1).

3. Role Of Prostaglandin F2 Alpha And Gonadotrophin Releasing Hormone In Induction Of Oestrus In Postpartum Anoestrus Nili Ravi Buffaloes During Summer

by Liaqat Ali | Dr.Imtiaz Hussain Khan | Dr.Muhamad | Dr.Muhammad Aleem | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 1998Dissertation note: In this experiment, twenty four Nili-Ravi buffaloes 60 days postpartum having history 60 normal postpartum were used. These buffaloes were randomly divided into two equal groups (n=12). Treated buffaloes received single injection of 5mg etiproston at 60 days postpartum. And at 78 days postpartum a single injection of gonadotrophin releasing hormone (GnRH) 0.05mg intramuscularly was given to only those buffaloes which did not showed oestrus after etiproston injection. The observation on different aspects like oestrus response, heat detection, interval to onset of oestrus, artificial insemination and pregnancy diagnosis were studied. The buffaloes in group-1, 75% (n=9) exhibited oestrus. The PGF2atreated buffaloes 16.66% (n=2) exhibited oestrus and GnRH treated buffaloes 70% (n 7) exhibited oestrus. The buffaloes in group II 16.66% (n2) exhibited oestrus. The behavioural signs noted were; acceptance of teaser bull, frequent urination, bellowing, licking of external genitalia, mounting by other buflaloes, and raised tail. The physical changes recorded were, uterine tone, swollen vulva, vaginal mucous discharge and vaginal hyperaemia. Oestrus symptoms were more pronounced in treated group than control groups. The buffaloes in treated and control groups, the average number of days from parturation to first oestrus were 81±3.61 and 107±20 days respectively, that was significant (P <0.05) difference between two groups. In PGF2a and GnRH treated buffaloes exhibited oestrus during 62.5±0.5 days and 86.28±1.21 days postpartum respectively. In treated group, the interval to onset of oestrus after injection was 6.22±1.16 days. In control group days from beginning of experiment to onset of oestrus were 47±2 days. Duration of oestrus in treated group was 24±2.11 hours. In PGF2cz and GnRH treated animals the duration of oestrus was 25±3 hours and 24.28±2.67 hours respectively. In control group the duration of oestrus was 18±2 hours. The pregnancy rate in treated and control groups were 44.4% and non of control group conceived. Availability: Items available for loan: UVAS Library [Call number: 0669,T] (1).



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