1.
Principles of Biochemistry and Bio-Physics
by Naz, Shiv.
Edition: 1st ed.Material type: ; Literary form:
not fiction
Publisher: New Delhi: Random Publications, 2014Availability: Items available for loan: UVAS Library [Call number: 574.194 NAZ 1sted. 2014 29748 Biochemistry] (1).
2.
Prepartyion And Evaluation Of Cheddar Cheese From Lactobacillus Acidophilus And Bifidobacterum Bifidum
by Irum Naz | Prof. Dr. Muhammad Abdullah | Dr. Muhammad Ayaz | Prof. Dr.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2010Dissertation note: Cheese is a food product which is made by fermenting milk by adding certain starter culture. It is highly proteinaceous and contains high content of fat. It has many flavors but Cheddar Cheese is most popular among all of its kinds. By adding probiotics in cheese it would be added to the group of functional foods.
Probiotics are live micro organisms that are useful for human body if taken in proper amount. There are many food products which contains probiotics like yogurt and fermented milk but the main drawback of these products are their shelf life which is very short and probiotics does not remain viable for longer period of time .
The study was formulated to manufacture probiotic cheddar cheese, chemical and sensory evaluation of probiotic cheese and to check the viability of probiotics. The data was statistically analyzed for the effect of storage and treatment on probiotic viability by using Duncan's test.
The probiotic cheddar cheese was manufactured at Haleeb Foods. Starter culture and rennet was provided by Haleeb foods and probiotic cultures in freeze-died form were obtained from Christian Hansen, Denmark. The starter culture alone and in combination with different concentrations of probiotics were added to milk for cheese making. After completing all the steps involved in its preparation, cheese were removed from mould, packed in vacuum cryovac packaging and were placed for ripening for 90 days at 4ºC.
Sensory evaluation of Cheddar Cheese was done by a team of panelists to check its flavor, colour, taste, texture and overall acceptability at every fifteen days interval during maturation time. There is a significant effect of different levels of probiotics on flavor, color, taste, texture and overall acceptability of Cheddar Cheese. Storage has also shown a significant effect on the sensory parameters of cheese.
Chemical tests like pH, fat, moisture, protein, salt and acidity concentrations were also carried out after fifteen days interval at the laboratory of Department of Food and Nutrition, University of Veterinary and Animal Sciences Lahore. There is a significant effect of pH, acidity, fat and protein on cheddar cheese. As pH lowers with the passage of time its acidity increases. So there is significant effect of storage on different treatment levels. The levels of Lactobacillus acidophilus specifically have shown an increased acidity.
Viability of probiotic bacteria was checked at 15 days interval at the Laboratory of Department of Microbiology, University of Veterinary and Animal Sciences, Lahore. During ripening Bifidobacterium bifidum has shown a good viability level of 107 and 108 cfu/20g of cheese. But on the other hand lactobacillus acidophilus have sustain its numbers for about two weeks and then decreased to a very low level. So there is a significant effect of viability on both strains during the storage. There is non significant effect of levels on viability of probiotics.
The statistical analysis used was two way analysis of variance to check the treatment and storage effect on probiotic cheddar cheese by following the method (Steel et al., 1997). Significance of means was compared by using DUNCAN'S multiple range tests (1955).
The research has enabled us to conclude the outcomes that the Cheddar Cheese is a good vehicle for delivery of probiotics but the lactobacillus strain needs more improvements its viability is concerned.
Availability: Items available for loan: UVAS Library [Call number: 1206,T] (1).
3.
Characterization Of Indigenious Species Of Mycotoxins Producing Aspergilli
by Gull Naz | Dr. Aftab Ahmad Anjum | Prof. Dr. Khushi Muhammad.
Material type: ; Format:
print
Publisher: 2010Dissertation note: Pakistan's economy is based on agriculture. Agriculture crops are harvested and stored in feed mills for production of thousands ton of feed for livestock as well as poultry through out the year. In Pakistan, July and August are hot and humid months during which moulds grow abundantly on the heaves of wheat! rice/maize straw and feed ingredients and produce variety of toxins.
Present study has been designed to explore different groups of moulds prevailing in and around Lahore city in each month of the year. Samples of soil and air were collected from ten different places of Lahore city.
A total of 240 samples were cultured on a common Saboraud's Dextrose Agar to get single colonies of each mould. These single colonies were identified by colony characters, slide cultures and biochemical tests. Mycotoxin producing Aspergilli were isolated by culturing on specified media and placing the cultures under Wood's lamp. Mycotoxin productions potential were assessed by extracting mycotoxins of these Aspergilli. Mycotoxins produced by the Aspergilli were identified and purified through Thin Layer Chromatography. These mycotoxins were then quantified through High Performance Liquid Chromatography.
The identified and purified mycotoxins can be used as standards. Reference standards are important and critical for qualitative and quantitative detection of mycotoxins in field samples screening. Presently mycotoxin is a ban item. The occurrence of toxinogenic Aspergilli have economic impact directly on livestock and poultry products export.
Availability: Items available for loan: UVAS Library [Call number: 1217,T] (1).
4.
Serum Biochemical Metabolites, Electrolytes And Minerals Profiling In Heat Stressed Donkeys (Equus Asinus)
by Naz Fatima | Dr. Muhammad Shahbaz Yousaf | Prof. Dr. Habib-ur-Rehman | Faculty of Biosciences.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: Background
In developing countries, equines are used as a companion animal, for transportation, carrying goods, pulling carts and to ride. Heat stress (HS) is the major problem of tropical and subtropical region that adversely affects the performance of livestock by altering the physiological indices.
Objective
The current study is designed to report the dynamics of different physiological indices including serum electrolytes, minerals, biochemical metabolites, hepatic enzymes and thyroid hormones in heat stressed (HSd) donkeys (Equus asinus).
Materials and Methods
A total of 20 donkeys were involved (10 healthy and 10 clinically diagnosed as HSd), irrespective of their gender and age, in the current study. The health of the donkeys was evaluated by observing vital signs including body temperature, respiratory rate, heart rate and hematocrit. Blood was collected from each animal during summer season in August and serum was harvested by centrifugation, of clotted blood, at 4,000 rpm for 20 minutes. Serum samples were stored at -20°C for analysis. Serum electrolytes and minerals were determined using flame photometry and atomic absorption spectrophotometer, respectively, after wet digestion of serum. Serum biochemical metabolites and hepatic enzymes were evaluated using commercial kits, spectrophotometrically. Serum thyroid hormones concentrations were determined using commercial ELISA kits. Normal distribution of the data was confirmed by Shapiro-Wilk test. Data was analyzed using Independent Student's t-test by employing Statistical Package for Social Science (SPSS). Data was presented as means ± SE. Differences were considered significant at P < 0.05.
Results
Results revealed significantly higher heart rate, respiratory rate, rectal temperature and hematocrit in heat stressed donkeys. Results revealed that serum sodium and potassium concentrations were significantly lower and serum calcium, magnesium and iron concentrations were significantly higher in heat stressed donkeys. However, there was no significant difference in Na/K ratio and serum chloride in HSd donkeys. Results revealed significantly higher serum total proteins, albumin, globulins concentrations, A/G ratio, LDL-cholesterol levels, T3, T4, AST, ALT, and ALP activity and homocysteine concentrations in heat stressed donkeys. However, serum HDL-Cholesterol and triglycerides levels were significantly lower. No significant difference in concentrations of serum glucose and cholesterol was observed.
Conclusion
Heat stress decreases electrolytes concentration and increases homocysteine and LDL-cholesterol levels that may account for increased risk of cardiovascular manifestations.
Availability: Items available for loan: UVAS Library [Call number: 1437,T] (1).
5.
Nutritional Characterization Of Spinach, Fenugreek And Mustard Leaves And Evaluation Of Their Glycemic Index
by Tahira Naz | Dr. Muhammad Nasir | Dr. Ali Raza | Prof. Dr. Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1512,T] (1).
6.
Effect Of Rbst Treatment On Antral Follicular Count Plasma E2 P4 Profile And Esteus Behavior In Postpartum Nili Ravi Buffaloes
by Sadia naz | Dr. Amjad riaz | Dr. Jawad nazir | Prof. Dr. Nasim Ahmad.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2055,T] (1).
7.
Detection Of Vivax Malaria And Under Local Climatic Conditions
by Sarwat naz | Prof.Dr. Azhar maqbool | Prof. Dr. Mansur ud din ahmad.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2060,T] (1).
8.
Evaluation Of Mutagenicity And Genotoxicity Of Furosemide And Propranolol Alone And In Combination Using Ames Test And Single Cell Gel Electrophoresis (Comet) Assay
by Sadaf Naz | Dr. Muhammad Adil rasheed | Prof. Dr. Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2158,T] (1).
9.
Isolation and Identification of Parasites From Available Raw Vegetables
by Sawera Chaudhry (2008-VA-189) | Prof. Dr. Azhar Maqbool | Dr.Sarwat Naz | Dr. Muhammad Latif | Dr. Muhammad Avais.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Blank CD. Availability: Items available for loan: UVAS Library [Call number: 2232-T] (1).
10.
Impact Analysis Of Quality Control Practices In Medical Diagnostic Labs Operational In District Lahore
by Tahira Naz Saif (2009-VA-204) | Dr. Ali Ahmed Sheikh | Dr. Ali Ahmed Sheikh | Dr. Muhammad Yasin Tipu.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Medical laboratory services are essential to patient care as laboratory results influence 70% of diagnosis. When tests are performed, there is always some level of inaccuracy. The challenge is to reduce the level of inaccuracy as much as possible. In orders to reduce these errors throughout the lab testing cycle, it is very important to maintain the quality of diagnostic lab. For this purpose standards should be set in the laboratory. These standards have the general requirement for effective management and competent testing.
In this study 5 public and 4 private sector medical diagnostic labs in district Lahore were selected. Permission from the competent authorities was taken and information was gathered through questionnaire including parameters such as lab building design, human resource of the lab, equipment and consumables, quality control measures adopted in the labs, environmental monitoring, waste management and customer care covering factors like recruitment policy of staff, Job descriptions of the staff, trainings conducted in the lab, SOPs, quality control and quality assurance program, feedback system, corrective and preventive action implemented in lab, result reporting etc.
The data was analyzed statistically through frequency distribution by using Statistical Package for Social Sciences (SPSS) version 18.0 for development of Graphs and Tables.
This study was conducted to help to evaluate the quality system of medical diagnostic labs and to identify the critical points which affect the quality of the testing and diagnosis and are being ignored. Information regarding different parameters was summarized in tabulated form by calculating percentage values. Two types of comparison were done. Firstly percentage value for each lab is calculated to check which labs fulfill maximum requirements of the parameters described. Second comparison was done to find out the percentage of the labs out of total selected labs, which fulfill different factors of the parameters described. After statistical analysis using chi square results showed that there is no significant difference between private sector labs and public sector labs.
In the first parameter, lab building design lab 1 and lab 9 got 100% quality points and lab 6 and lab 8 got minimum scores of 20%. In second parameter, human resource of lab 1, 2 and 3 got 100% quality points. Lab 6 and 8 got minimum quality points of 33%. When third parameter equipment and consumables was investigated it was found that lab 7 and lab 9 100% fulfill this parameter and lab 6 and lab 8 fulfill this parameter only 16.6%. In fourth parameter, quality control practices implemented in labs; lab 2 got 86% scores and lab 1 got 82% while lab 8 got minimum quality points of 22%. In fifth parameter, waste management of the lab; lab 4 got maximum quality points of 83% and all other labs got the same score of 66.6% in this parameter. When sixth parameter, environmental monitoring was investigated. It was found that lab 2 and lab 9 monitor the environment of the lab only 66.6% and in lab 8 environmental monitoring is not done. In seventh parameter, customer care lab 9 got 100% scores and labs 3, 4, 5 and 6 got 50% scores.
Conclusion:
Results show that there is no significant difference between government and private sector labs. Labs do not pay attention to the quality control measures and use same approaches of quality control in their system.
Availability: Items available for loan: UVAS Library [Call number: 2317-T] (1).
11.
Antibacterial And Cytotoxic Evaluation Of Sequential Extracts Of Ocimum Basilicum Leaves Against Common Poultry Pathogens
by Shomaila Naz (2013-VA-1001) | Dr. Muhammad Ovais Omer | Dr. Muhammad Adil Rasheed | Prof. Dr. Aftab Ahmad Anjum.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Antimicrobial compounds having plant origin inhibit bacteria through different mechanisms and can be used for the treatment of infections against resistant microbes. Majority of antibacterial drugs in clinical use are derived from natural origin. Hence, the present study is designed for antibacterial and cytotoxic evaluation of different extracts of Ocimum basilicum seeds against common poultry pathogens.
The four sequential i.e. hexane, chloroform, ethanol and aqueous extracts of Ocimum basilicum leaves and seeds were prepared by soxhlet extraction. Antibacterial activity of these extracts was determined by agar well diffusion method against Staphylococcus aureus, Clostridium perfringens type A, Escherichia coli, Salmonella enterica and Haemophilus paragallinarum. Zone of inhibitions were determined by well diffusion method. MICs of plant extracts were determined by micro broth dilution method. Cytotoxic activity was evaluated by applying MTT assay on Vero cell lines. All the results were statistically analyzed by one way ANOVA and compared means by Duncan’s multiple range of posthoc test at significance level of P≤0.05.
The results of zone of inhibitions showed by Ocimum basilicum leaves and seeds extracts ranging from 11.33-20.0 mm values of MIC results ranging from 4.889 μg/ml-2500 μg/ml of hexane, chloroform and ethanol. The aqueous extract of Ocimum basilicum have no activity against any bacterial pathogen. Ethanol extract of Ocimum basilicum leaves was cytotoxic at 500 μg/ml. Hexane extract of Ocimum basilicum seeds was cytotoxic at concentration ≥625 μg/ml, chloroform at concentration ≥19.53 μg/ml and ethanol extract at concentration ≥750 μg/ml.
The indigenous plant Ocimum basilicum have antibacterial activity against common poultry pathogens and helpful to develop new drug from plant origin. Availability: Items available for loan: UVAS Library [Call number: 2443-T] (1).
12.
Isolation And Molecular Characterization Of Causative Agent Of Equine Strangles
by Tayyaba Naz (2008-VA-235) | Prof. Dr. Khushi Muhammad | Dr. Aamir Ghafoor | Prof. Dr. Aneela Zamir Durrani.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Strangles is an important infectious and contagious disease of horses that affects upper respiratory tract. This disease is very much prevalent in Pakistan. Disease can be controlled by early diagnosis, strict quarantine measures and proper immunization. Disease is characterized by nasal discharge, submandibular lymph node swelling, raised temperature and anorexia. The disease can terminate into encephalitis, purpura hemorrhagica and bastard strangles. 20 nasal swabs as samples were collected from horses showing signs of nasal discharge or swollen lymph nodes from Remount depot Sargodha, 10 samples from clinically sick horses and 10 from apparently healthy horses with the history of disease. Samples were swabbed on to blood agar with 5% defibrinated blood of sheep. Isolated hemolytic colonies were undergone biochemical testing with the help of API strep 20 kit. Samples which appeared as streptococcus equi through biochemical testing were subjected to molecular amplification by targeting two genes. Two different PCR were performed PCR 1 targeted the Sod A gene this gene is present in Streptococcus equi and PCR-2 targetted SeM gene this gene is specific for Streptococcus equi subsp. Equi only. 5 samples were confirmed positive for Streptococcus equi through biochemical and molecular testing. SDSPAGE on the isolated bacterial samples were performed and it appeared as no protein diversity was observed among different isolates. However the protein pattern varied with number of passages as less number of bands appeared from older cultures. Availability: Items available for loan: UVAS Library [Call number: 2445-T] (1).
13.
Optimization Of Multiplex Pcr For Simultaneous Detection Of Bacterial And Viral Water Borne Pathogens
by Faiza Naz (2010-VA-288) | Prof. Dr. Masood Rabbani | Dr. Fareeha Akhtar | Dr. Sana Ullah Iqbal.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Waterborne illness is a serious issue throughout the world. Bacteria such as Salmonella spp., Shigella spp., E. coli spp. and from Viruses mostly Rotaviruses are involved in various waterborne outbreaks due to usage of contaminated water because of poor sanitation system mixing of waste material and fecal material with water, which can be transferred to human body by consuming such contaminated water Detection of these bacteria and virus from various foods by conventional method is not easy. Conventional methods are time consuming laborious and expensive. Now multiplex PCR is widely used for rapid detection of waterborne pathogens. The method is more sensitive and specific and can detect more than one pathogen in one single reaction mixture. This experimental design is developed to optimize the multiplex PCR reaction for detection of Salmonella spp., Shigella spp., Escherichia coli spp. and Rota virus.
ATCC culture of Salmonella spp., Shigella spp., Escherichia coli spp., revived using standard culturing technique and multiplex PCR is optimized to amplify four different microbial genes simultaneously. A total 100 samples obtained from 10 towns, of Lahore. The samples were processed for multiplex PCR for detection of E. coli spp., Salmonella spp., Shigella spp. and Rota virus directly from water samples.
With the amplification of 4 bacterial and viral genes simultaneously multiplex PCR was optimized. Water samples were obtained, to check the strength of planned experiment in the field. The samples were processed for and multiplex PCR for direct detection of Salmonella spp. and E. coli spp. directly from water samples.
Similarly multiplex PCR was optimized with 3μl DNA template of each microbe , 56oC annealing temperature , 20pmol of every primer and 25μl of multiplex master mix.
Multiplex PCR is more sensitive and specific. It is also time redeemable technique because conventional culturing method requires several days for the detection of waterborne pathogens but this technique wants expertise.
This study was helpful to establish an optimized Multiplex PCR for the rapid and simultaneous detection of waterborne pathogens. Availability: Items available for loan: UVAS Library [Call number: 2612-T] (1).
14.
Muslim Shakhsiat ka Encyclopedia
by S.M. Naz.
Edition: 1stMaterial type: ; Literary form:
not fiction
Publisher: Lahore: Sheikh Ghulam Ali and Sons ndAvailability: Items available for loan: UVAS Library [Call number: 297.1 Naz 17441 1st nd Islam] (1).
15.
Zual Jilal wal Ikram
by Shahida Naz Qazi.
Edition: 1stMaterial type: Publisher: Lahore: Islamic Publications; 1999Availability: Items available for loan: UVAS Library [Call number: 297.1 Shahida 17503 1st 1999 Islam] (1).
16.
Nutritional Assessment Of Hypertensive Patients And The Impact Of Nutrition Education On Their Dietary Practices In Contrast To Dash Diet
by Farah Naz (2012-VA-401) | Miss Tahreem Hussain | Dr. Sanaullah Iqbal | Dr. Mamoona Chaudary.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Hypertension is a common public health problem in developing countries. It leads to
further complications such as coronary heart disease, stroke and renal disorder. The main reason
is unawareness, poor dietary habits and worsened lifestyle in hypertensive patients. Nutritional
education at the right time may prevent other risk factors associated with hypertension.
Nutrition education have a positive impact on dietary practices of hypertensive patients
for secondary prevention.
A total of 200 hypertensive patients was purposively selected for this study. The willing
patients was informed about the purpose of study and consent was taken. The pretesting of the
hypertensive patients was administered about their usual dietary habits through questionnaire,
Food Frequency Questionnaire and 24-hour dietary recall. A total of 60 hypertensive patients
came with chronic diseases so they were excluded from the Research. Lessons and handouts on
nutrition education were given to the patients for a period of four weeks (1 month). After
completion of one month of nutrition education, post-testing was administered through filling up
of the same Questionnaire, Food Frequency Questionnaire and 24-hour dietary recall to assess
the improvement in dietary habits of hypertensive patients and results were calculated.
SPSS v. 21 was used for the compilation and analysis. Wilcoxon signed ranked test and
Paired t-test was applied to analyze the effectiveness of nutritional education on dietary habits of
hypertensive patients. Level of significance was p ≤ 0.05.
The present study helped to assess the effectiveness of nutrition education on improving
the knowledge and dietary practices of hypertensive patients. Availability: Items available for loan: UVAS Library [Call number: 2661-T] (1).
17.
Cross Sectional Survey Of Avian Infleunza In Poultry Butchers Of Sentinel Live Bird Markets In Lahore District
by Gul Naz Namat (2014-VA-1120) | Dr. MamoonaChaudhary | Dr. Hassan Mushtaq | Dr. Shafqat Fatima Rehmani.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: 6.1 Background:
Influenza is a highly contagious, acute illness in humans. Influenza viruses have negative-sense RNA genomes and are placed in the Orthomyxoviridae family grouped into three types A, B and C on the basis of the internal nucleocapsid or the matrix protein. Droplet and airborne are the most common modes of transmission. In Humans infection appears to be direct or indirect exposure to infected live or dead poultry or contaminated environments, such as live bird markets.
6.2 Hypothesis:
Avian influenza virus is prevalent in poultry butchers working in live bird markets with domestic and pet, wild, exotic birds.
6.3 Parameter/Methodology:
A cross sectional survey of poultry butchers in Lahore was conducted in order to determine seroprevalence of avian influenza Disease. A target population was the poultry butchers/retailers in the District Lahore. A study population was the apparently healthy butchers in Lahore. A sample of 300 butchers was collected. Blood sample from apparently healthy butchers was collected from brachial veins as described in WHO (2010). Three ml blood was collected in the syringe and was allowed to clot to separate serum. Collected sera was stored in freezer at -70°C for further laboratory analysis. Data was gathered by simple random sampling technique. A total of 300 samples were collected. Haemagglutinationinhibition (HI) test applied to detect antibodies sensitivity. The test was followed as described by WHO (2013).
6.4 Statistical Design:
The weighted proportion estimate with 95% Cl (confidence intervals) of the overall seroprevalence was computed by using R-software. Logistic regression was conducted to estimate the effect of each study variable on the outcome.
6.5 Outcomes:
Out of 300 blood samples of butchers of sentinel live bird markets, 171 blood samples are sero positive for H9 virus after HI Testing. The sero prevalence of H9 virus in butchers of live bird markets is found to be 57 %. The results of H5 and H7 are found to be negative.
In univariable analysis, following risk factors which were significant as per criteria of selection in current study (p-value < 0.25); Education, Age, Smoking, Sticks smoke/day, Years of smoking, Having chronic disease, Birds sold/day, Keep birds at home, Access of stray dogs, Access of stray cats, Wash instruments after slaughtering, Do not clean cutting boards, Wearing of aprons.Multivariate analysis determined one factor i-e having birds other than broiler as significant factor having p-value < 0.001.
Availability: Items available for loan: UVAS Library [Call number: 2804-T] (1).
18.
Na Cheez
by Meena Naz.
Material type: ; Literary form:
not fiction
Publisher: 2004Availability: No items available Checked out (1).
19.
Syed Ahmad Khan
by Naz.
Material type: ; Literary form:
not fiction
Publisher: 1971Availability: Items available for loan: UVAS Library [Call number: 954.0317 Naz 9633 1971 History] (1).
20.
Seerat Ibne Sham
by M. S Naz.
Material type: ; Format:
print
; Literary form:
not fiction
Availability: Items available for loan: UVAS Library [Call number: 297.163 Naz 21663 1st Islam] (1).
21.
Umar Bin Abdul Aziz
by Naz.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1970Availability: Items available for reference: Old Books [Call number: 922.97 History 9640] (1).
