1.
Effect Of Mentofin On Serum Biochemistry And Antibody Response Of Broiler To Avian Influenza H9N2
by Rizwana sultan | Prof. Dr.Asim Aslam | Dr Gulbeena Saleeem | Dr. hafsa zaneb.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1907,T] (1).
2.
Comparison Of Ameliorative Potential Of Sacccharomyces Cerevisiae And Bentonite Clay On Pathological Effects Induced By Aflatoxin in Broilers
by Muhammad Saqlain | Dr. Ishtiaq ahmad | Dr. Gulbeena saleem | Prof. dr. Aftab.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2014,T] (1).
3.
Pathologenesis Of Anaplasmosis In Balkhi Sheep And Their Confirmation With Polymerase Chain Reaction
by Ajab Khan | Dr. Muti ur Rehman Khan | Dr. Gulbeena Saleem | Dr. Saima.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2145,T] (1).
4.
Detection Of Brucella Species From Aborted Bovine Fetuses Using Amos Pcr And Immunohistochemistry
by Muhammad Naveed Anvar (2008-VA-310) | Dr. Raheela Akhtar | Dr. Gulbeeena Saleem | Dr. Jawad Nazir.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2015Dissertation note:
The economic importance and public health concern of bovine brucellosis enlist it in the world top priority disease to be eliminated by WHO (World Health Organization). Bovine brucellosis is caused by three Brucella species including B. abortus, B. melitensis and B. suis. Although the literature explains that the incidence of B. abortus is higher in bovines but still we need to know the prevalence of other species particularly B. melitensis in Pakistan due to its zoonotic aspect which makes it more important for study. Unfortunately in Pakistan the status of B. abortus and B. melitensis in bovines is unknown. It is need of hour to determine the exact prevalence of B. abortus and B. melitensis in bovines for disease eradication in animals, to control its transmission in humans and to determine the reasons behind vaccine failure in bovines.
B. abortus and B. melitensis could be presents in aborted bovine samples. AMOS PCR can be better tool than immunohistochemistry for the detection of B. abortus and B. melitensis from aborted bovine samples. (Hypothesis)
A total of 60 tissue samples (lung, liver and stomach) from aborted bovine fetuses were collected from farms with history of abortion and suspected brucellosis in and around Lahore district. The samples were subjected to AMOS PCR and immunohistochemistry for detection of B. abortus and B. melitensis. Brucella abortus and Brucella mellitensis species specific primer were used to get the desired base pair. The genomic region of B. abortus IS711was amplified at 498bp.
From present study it can be concluded that brucellosis is present in cows and buffaloes at district Lahore and it is more in cattle as compared to buffaloes. Therefore an immediate actions and policies are required to be implemented for the preventing spread of the disease to the other animals and human. For the diagnosis of Brucella species AMOS PCR and immunohistochemistry were used and the results showed that Brucella abortus were more as compared to other species in aborted bovine tissues. The results also showed that the sensitivity and specificity of AMOS PCR is more than immunohistochemistry.
Availability: Items available for loan: UVAS Library [Call number: 2320-T] (1).
5.
Amelioration Of Pathological Effects Of New Castle Disease By Aloe Vera
by Sayyed Raza Ali Shahid (2014-VA-515) | Prof. Dr. Zafar Iqbal Chaudhry | Dr. Gulbeena Saleem | Prof. Dr. Aftab Ahmed Anjum.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Poultry industry has many threats from the infectious diseases. Newcastle disease is the most important disease of the poultry as it is distributed all over the world and it can cause huge economic losses in poultry industry. It is caused by the Newcastle disease virus (NDV) that can infect about 240 species of birds. Newcastle disease cause immune suppression in birds. It is reported that supplementations of Aloe vera enhances the immune status and reduce inflammation. So this research project was design to observe the effect of Aloe vera on lymphoid organs, growth performance and antibody response in Newcastle disease challenge birds. For this research a total of 120 broiler chicks were divided into four groups A, B, C, and D. Group A was control group while B and C were treated with 2 percent aqueous extract of Aloe vera. Group C was also vaccinated against New Castle disease. Aloe vera was given to group B and C from day one to end of study trial. Both of the groups were challenged with ND virus at day 21. Group D was vaccinated against ND and was challenged with ND virus at day 21 without supplementation of Aloe vera. Blood samples were collected at day 1, 7, 20, 24, 26 and 28 to determine the antibody titer against ND. Highest antibody titers were observed in group C as compared to all other groups which was vaccinated against ND along with supplemented with Aloe vera. For gross pathology and histopathology, lymphoid organs were collected at day 24, 26 and 28..The average feed intake of group A and D was significantly higher than group B and C before challenge of virus but the body weight gain of 2% Aloe vera supplemented broiler was significantly (p<.05) higher than without treatment of Aloe vera. The FCR of birds supplemented with Aloe vera treatment was significantly different from the birds without Aloe vera treatment. The FCR value of group C and B was higher than A and D.
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A significant difference was observed in the weight of lymphoid organs of birds treated with Aloe vera as weight of organs was less in group C followed by group B, group A and group D. This was due to anti-inflammatory effects of Aloe vera. Microscopic examination revealed congestion, depletion of lymphocytes, dysplasia of thymic lobules, thinning of cortex, focal necrosis, disappearance of lymph follicles and inter-follicular edema like lesions within lymphoid organ of the groups challenged with Newcastle disease virus. However, cellular hypertrophy and decreased lymphocytes population were prominent changes in lymphoid organs of broiler treated with 2% Aloe vera. To check the virucidal effects of Aloe vera, a separate experiment was conducted in which 9 day old embryonated eggs were inoculated with ND virus along with 2 percent Aloe vera gel extract after incubation at 37Cº for an hour in group A while only ND virus was inoculated in group B. Candling was performed to see the survival of embryos in both groups which revealed a significant difference i.e. 16 percent embryos were found dead in group A while 80 percent was found dead in group B. Later on the amnioallantoic fluid of the eggs was used for spot Haemagglutination test. Group A showed less agglutination activity then group B. From this study it was concluded that Newcastle disease caused immune suppression and damage of vital organs in broiler while Aloe vera have immunomodulatory, anti-inflammatory and antiviral effects as it raised antibody titer against Newcastle disease virus and lowered the inflammatory processes along with inactivation of ND virus. It also promotes growth performance of broilers and helps the birds to survive against lethal ND disease. Availability: Items available for loan: UVAS Library [Call number: 2525-T] (1).
6.
Study On The Pathogenesis Of Field Isolate Of Salmonella Pullorum In Experimentally Infected Broiler Chicken
by Muhammad Zeeshan (2014-VA-535) | Dr. Muti-ur-Rehman Khan | Dr. Gulbeena Saleem | Prof. Dr. Aftab Ahmad Anjum.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Poultry is one of the well organized sectors in Pakistan and generates employment for up
to 1.5 million people. As use of modern techniques and medicine has reduced a risk lot but it is
still facing threat from many diseases. One of these is Salmonellosis which is and hatchery borne
infection and it also has a zoonotic importance. This organism is gram negative bacterium, non
acid fast, uniform staining and non spore forming bacillus.
Pullorum disease produced by it is also known as bacillary white diarrhea due to whitish
droppings produced by birds. Day old chicks are affected because it can be transmitted vertically.
Infected fomites and utensils also play a role in the transmission of infection. Growth of S.
pullorum rapidly occurs upon beef agar on nutrient media and growth is best at 37 °C. Selective
media such as selenite, tetrathionate broths and differential media such as MacConkey, bismuth
sulfide and brilliant green agar are best for the proper growth of organism.
The organism was isolated from the 30 different infected samples of broiler birds taken
from different farms and was grown upon the selective media. The colonies were picked from
the cultures and were subjected to PCR for the further process. After confirmation with PCR,
inoculums was prepared with a bacterial load of 2 x 107 (CFU) in 0.5 ml of normal saline at the
ph of 7.2.
Chicks were randomly divided in to three groups as group 1, 2 and 3, each group
containing 30 chicks. Group 1 was infected with field strain of S.pullorum, Group 2 with
vaccinal strain and Group 3 was set as control group and no infection was given to it. Samples
were collected from birds at 10th, 15th, 20th and 25th days of age. Observations were made upon,
clinical signs, histopathological lesions, mortality and morbidity rate, FCR and gross
pathological lesions.
Clinical signs included were anorexia, depression, huddling, labored breathing, loss of
feed and water intake, ruffled feathers, reduced mean body weights, low FCR and pasty vent.
Gross and histopathological lesions were much observed and were prominent in the birds
affected with field strain of S. pullorum. One Way-ANOVA was used for statistical analysis in
between the groups. Gross pathological lesions included were liver congestion 55%, cheesy
material in caeca 70% in the birds which were affected with field strain of S. pullorum.
Histopathological findings in the birds affected with field strain were monocytes in spleen,
leukocytes in intestine and heterophils in liver. Low FCR was recorded in infected birds of
Group 1 and was 1.63 at 25th day. Mortality rate in the birds affected with field strain was
maximum 43.33% and morbidity rate was 73.33% at the 10th day of age. Birds of group 2 were
less affected and only showed medium level of lesions. No specific lesions were seen in group 3.
The total prevalence of of S.pullorum after conformation with PCR was 20% out of total 30
infected samples. The organs for histopathology taken were liver, spleen, caeca and intestine and
were preserved in 10% formalin prior to the infection.
The overall pathogenesis of experimental bacteria after given through oral route was that
it first localizes in digestive tract from where it enters into the blood stream and invades in the
different organs and tissues at different time intervals producing lesions and immunological
response. Availability: Items available for loan: UVAS Library [Call number: 2693-T] (1).
7.
Pathological Studies On Contagiouscaprine Pleuropneumonia In Small Ruminants Of District Dera Ismail Khan, Khyber Pakhtunkhwa
by Hamid Niaz (2008-VA-163) | Dr. Muti Ur Rehman | Dr. Gulbeena Saleem | Prof. Dr. Aneela Zameer Durrani.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: These experiments presented the pathological studies on Contagious caprine pleuropneumonia in Distract Dera Ismail Khan at Govt. slaughter house through gross pathology, hematology, competitive ELISA, histopathology and molecular identification (PCR). For this purpose the blood and tissue samples were collected from contagious caprine pleuropneumonia suspect at govt slaughter house Dera Ismail Khan. On the basis of clinical examination, the animals were showing the elevated temperature, nasal discharge, painful cough, dyspnea and weakness.
On postmortem examination the lungs showed congestion and marbled appearance (Figure no 4.1). There was straw color pleural fluid in the chest cavity (Figure no 4.2). Total of 50 samples 25 blood and tissue samples of sheep and 25 blood and tissue samples of goat were collected for the different parameters of the study. The hematology was performed at SEENA Lab in Distract Dera Ismail Khan. The hematology data was further compared and analyzed by using statistical analysis (independent t-Test). RBCs values of positive and negative cases of CCPP in goats were 9.50±0.27 and 3.15±0.39respectively. There is significant difference (p<0.05) between positive and negative cases of CCPP in goats regarding RBCs values. WBCs values of positive and negative cases of CCPP in goats were 8.31±0.38 and 17.19±1.22respectively. There is significant difference (p<0.05) between positive and negative cases of CCPP in goats regarding WBCs values. PCV values of positive and negative cases of CCPP in goats were 4.03±.63 and 17.11±1.5respectively. There is significant difference (p<0.05) between positive and negative cases of CCPP in goats regarding PCV values. The MCH values of positive and negative cases of CCPP in goats were 110±30.6and 7.65±1.01 respectively.So there was
Availability: Items available for loan: UVAS Library [Call number: 2843-T] (1).
