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1. Immunohistochemical Detection Of Infectious Bronchitis Virus In Different Organs Of Experimentally Infected

by Mudassar Iqbal | Dr. Asim Aslam | Dr. Ishtiaq ahmed | Prof. Dr. Tahir.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1671,T] (1).

2. Pathobiologeical Studies Of Infectious Bursal Disease Virus (Ibdv) In Experimentally Infected Birds

by Maria Ali | Dr. Muti-ur-Rehman Khan | Dr. Aftab | Dr. Ishtiaq Ahmed.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2014Dissertation note: Poultry industry is rapidly growing under the shelter of livestock, with the contribution of 11.4% of value addition in GDP. However infectious and noninfectious diseases are major threat to the growth of poultry industry. Among the infectious diseases, infectious bursal disease (IBD) is one of the major malady which causes great economic losses to poultry in terms of both morbidity and mortality. IBD virus was first identified in 1962 in gumboro, since then the disease spread to all parts of world. Until 1980s IBD virus was efficiently controlled by use of vaccine however with the emergence of variant strains of virus it was become difficult to control the disease. Studies on molecular structure of IBDV revealed that it has a hyper variable region in VP2 gene. Mutations in this hyper variable region lead to emergence of different strain of virus and hence causing vaccine failure. In Pakistan there is an outbreak of disease each year despite of vaccination. So there was a need to characterize field isolate and vaccine isolate of IBDV to find any mutation in field isolate of virus. The current study was designed by keeping above factors in mind. Purpose of present study was to compare local field and vaccine isolate of virus. RNA was isolated by TRIzol method from both vaccine isolate and bursa collected from field. RNA was converted to cDNA by using oligoDT. In next step VP2 gene was amplified by using a set of primers P1 and P2. cDNA was digested by 2 enzymes BstN1 and MboI. Results of RFLP showed that there was a difference in genetic sequence of both strains. Howeverthe pattern of disease produced by both of the strains was same. Chicks were given experimental infection at age of 2 week and slaughtered after 3 days post infection. Gross and histopathological lesions were observed and compared to each other and also with negative control. Statistical analysis was done by using one way ANOVA followed by Duncan's multiple range test. Availability: Items available for loan: UVAS Library [Call number: 1770,T] (1).

3. Study Of Carrier Potential Of Newcastledisease Virus In Common Wild Bird Species

by Tahir Munir | Prof. Dr. Asim Aslam | Dr. Muhammad | Dr.Ishtiaq Ahmed.

Material type: book Book; Format: print Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1892,T] (1).

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