Effect Of Various Extenders On Physio-Morphological Characteristics And Freezability Of Buffalo Bull Semen
By: Liaqat Sarfraz
| Imtiaz Hussain Khan.
Contributor(s): Rashid Ahmed Chaudry | Saghir Ahmed | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1991Subject(s): Department of TheriogenologyDDC classification: 0233,T Dissertation note: The most important aspect of spermatozoa preservation in frozen state is the type of extender used. The selection of suitable extender is based on evaluation of fresh, diluted and frozen semen through several parameters.
In this study, buffalo bull, semen, extended in different extenders was evaluated for sperm motility percentage, progressive sperm motility (0-5) Live spermatozoa percentage and total sperm abnormalities percentage. For this purpose semen was collected from four Nili-Ravi Buffalo bulls and evaluated by using various tests for different physical parameters. Semen was extended in ratio of 1:10, using four extenders for freezing namely, Egg-yolk-Tes-Tris, Egg-yolk-citrate-taurine, Lactose-fructose yolkglycerol and Egg-yolk-Egg--yolk"Salts". Sperm concentration was 20 million per dose. One step extension of semen was done. Straw (0.5 ml) were used for packaging and freezing of semen. Freezing was carrid out by holding the straws at a level of 4 cm above the surface of liquid nitrogen for seven minutes. Frozen straws were stored in liquid nitrogen less than one hour (0 hour) and for 7 days. The various parameters were observed in fresh semen, after extension, just after freezing and after storage (7 days). Thawing of frozen was done at 37°C for 13 seconds.
Highly-significant difference (P<0.01) was observed in motility percent after dilution, equilibration, post-thaw sperm motility and freezing (0 hr) and storage (7 days). Average post-thaw motility was 43.10 ± 0.31, 40.35 ± 0.24, 38.53 ± 0.13 and 36.09 ± 0.20 in Ey-Test, EY-cit-tau, LFYG and EY-EY"Salts" respectively.
The statistical analysis revealed significant difference (P<0.01) in live- dead spermatozoa percentage, total morphological abnormalities due extender and freezing stages.
The non-significant difference (P<0.05) was observed in the progressive sperm motility grades due to stages of freezing and extender.
While considering the results, the extender Ey-Test, EY-cit-tati, LFYG found superior than EY-EY-"Salts" but maintaining high motility percentage of spermatozoa alongwith the minimal alteration of other seminal attributes before and after freezing and it can be used satisfactorily for the preservation of buffalo semen at -196°C.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0233,T (Browse shelf) | Available | 0233,T |
The most important aspect of spermatozoa preservation in frozen state is the type of extender used. The selection of suitable extender is based on evaluation of fresh, diluted and frozen semen through several parameters.
In this study, buffalo bull, semen, extended in different extenders was evaluated for sperm motility percentage, progressive sperm motility (0-5) Live spermatozoa percentage and total sperm abnormalities percentage. For this purpose semen was collected from four Nili-Ravi Buffalo bulls and evaluated by using various tests for different physical parameters. Semen was extended in ratio of 1:10, using four extenders for freezing namely, Egg-yolk-Tes-Tris, Egg-yolk-citrate-taurine, Lactose-fructose yolkglycerol and Egg-yolk-Egg--yolk"Salts". Sperm concentration was 20 million per dose. One step extension of semen was done. Straw (0.5 ml) were used for packaging and freezing of semen. Freezing was carrid out by holding the straws at a level of 4 cm above the surface of liquid nitrogen for seven minutes. Frozen straws were stored in liquid nitrogen less than one hour (0 hour) and for 7 days. The various parameters were observed in fresh semen, after extension, just after freezing and after storage (7 days). Thawing of frozen was done at 37°C for 13 seconds.
Highly-significant difference (P<0.01) was observed in motility percent after dilution, equilibration, post-thaw sperm motility and freezing (0 hr) and storage (7 days). Average post-thaw motility was 43.10 ± 0.31, 40.35 ± 0.24, 38.53 ± 0.13 and 36.09 ± 0.20 in Ey-Test, EY-cit-tau, LFYG and EY-EY"Salts" respectively.
The statistical analysis revealed significant difference (P<0.01) in live- dead spermatozoa percentage, total morphological abnormalities due extender and freezing stages.
The non-significant difference (P<0.05) was observed in the progressive sperm motility grades due to stages of freezing and extender.
While considering the results, the extender Ey-Test, EY-cit-tati, LFYG found superior than EY-EY-"Salts" but maintaining high motility percentage of spermatozoa alongwith the minimal alteration of other seminal attributes before and after freezing and it can be used satisfactorily for the preservation of buffalo semen at -196°C.
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