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Genotyping Of Hydatid Cyst And Itd Prevalence In Cattle,Buffalo And Human Beings

by Muhammad Nauman Zahid | Prf.Dr. Azhar Maqbool | Dr.Aftab | Dr.kamran Ashraf | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Nature of contents: biography; Literary form: Publisher: 2008Dissertation note: ACystic echinococcosis (CE) is the larval cystic stage (called echinococcal cysts) of a small taeniid-type tapeworm (Echinococcus granulosus) that may cause illness in intermediate hosts, generally herbivorous animals and people who are infected accidentally. Echinococcus granulosus has number of genetically distinct strains which are known to differ morphologically and epiderniologically. Out of 150 cattle and 150 buffalo examined only 42 Samples of hydatid cysts were collected from different organs i.e. livers, kidneys, lungs and hearts from Lahore abbatoir. From 42 positive samples, 25 cysts were found in cattle and 17 cysts were tound in buffalo. Prevalence of hydatidosis in cattle was recoreded as 16.66% and 11.33% in buffalo. Fertility and viability of the cysts was observed microscopically. Out of 25 cysts of cattle. nine were fertile and out of 17 cysts of buffalo, only five were fertile. Seroprevalence of hydatidosis in 150 butchers working in abattoir was also determined by the use of Latex agglutination test (LAT) kit for detection of hydatidosis. The prevalence of Echinococcus is 24% which was derived from serum analysis of butchers. DNA from hydatid cyst was extracted. Polymerase Chain Reaction was run on extracted DNA samples. Amplicon was run on 1% agarose for confirmation of size and specificity of product. Size of PCR product was approximately l300bp. Genotyping of Echinococcus granulosus was performed through Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP). The PCR-RFLP analysis of CO I gene of Echinococus was performed to confirm the strain of Echinococcus in cattle .The data obtained was analysed and it was concluded that the G5 strain of echinococus is prevalent in Cattle in Punjab area. It is hoped that the findings of the present study will be helpful for further planning about the control of the disease and correlating the prevalence in cattles,buffalos and butchers from the zoonotic point of view. According to the results, the PCR-RFLP analysis of samples of patients suspected for Echinococejis is a promising diagnostic method and also confirms the type of Echinococcits prevalent in that area and also enables an early direct detection of parasite DNA. This effort is a step to minimize the losses produced by this disease. Availability: Items available for loan: UVAS Library [Call number: 1097,T] (1).

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Optimization Of Loop-Mediated Isothermal Amplification (Lamp) For The Molecular Diagnosis Of Feline Babesiosis

by Muhammad Awais Salim (2012-va-606) | Dr. Muhammad Lateef | Prof. Dr. Azhar Maqbool | Dr. Muhammad Nauman Zahid.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Babesia is a worldwide tick borne hemoparasite causing Babesiosis, an important disease affecting a number of animals and attracting the researcher’s attention due to its zoonotic potential.Babesiosis in cats often presents as a chronic and low grade disease, however most common symptoms include anaemia, lethargy, weakness and rarely icterus and fever. Blood samples were collected from 100 domestic cats at Pet Center, UVAS, Lahore,from their ear tips and cephalic/saphenous vein. The blood will be immediately transferred to EDTA coated vacutainers. Stained thin blood smears were observed for intra-erythrocytic bodies and 45 samples were selected after screening. Blood in EDTA were tested for PCR (already optimized) in the Molecular Parasitology laboratory at UVAS, Lahore, to screen for B. felis. ExtractedDNA of confirmed B. felis samples were further processed forLoop-Mediated Isothermal Amplification (LAMP). LAMP primers weredesigned recognizing four sections of the B. felis gene. LAMP reactions of 25µL were standardized at 60°C temperature for 1 hour time using DNA extracted from blood samples of cats found positive on PCR. Briefly, the concentration of FIP and BIP were varied from 0.8µM to 2.4µM, Mg2+from 2mM to 4mM, betaine from 0.2 to 0.8M and dNTPs from 1mM to 4mM.The LAMP reaction was optimized at the final concentration of 0.2µM F3 and B3, 2.0µM of each of the FIP & BIP, 2mM for each dNTPs, 0.8M betaine, 1X reaction buffer, 1µl bst polymerase and 2µl DNA templates at 60°C. Availability: Items available for loan: UVAS Library [Call number: 2374-T] (1).

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