UVAS Library

Items in your cart: 0

Your search returned 2 results. Subscribe to this search

Not what you expected? Check for suggestions
Unhighlight
Select all Clear all | Select titles to:
1.
No cover image available
Studies On The Isolation And Serotyuping Of Salmonella From Mesenteric Lymmphnodes Of Sheep And Goats

by Ahmad Usman Zafar | Muhammed Ajmal | Ata-Ur- Rehman Rizvi | T.M Khan | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1986Dissertation note: Five hundred samples of mesenteric lymph nodes of sheep and goats , 20 from each species , slaughtered at Lahore abattoir, were bacteriologically examined to determine the Salmonella carrier state in healthy sheep and goats. Nine strains of Salmonellae belonging to two serological types i.e. Salmonella typhimurium (l.40%) and Salmonella anatum (0.40%), were isolated. The incidence of Salmonellosis in sheep and goats were recorded as 2.40% and 1.20% respectively. The media used for primary isolation and purification were Selenite broth, Bismuth sulphite agar, Brillient green agar, MacConkey agar and Nutrient agar. Results of Triple sugar iron agar and Urease test gave a preliminary confirmation of Salmonella organisms. Sugar fermentation reactions, Methyl red and Voges Proskauer tests were also employed for the confirmation of isolates. The Serological identification was carried out with the help of standard antisera manufactured by ' Research Institute of Infectious and Parasitic Diseases Sofia, Bulgaria. Further work on much larger scale is recommended. Availability: Items available for loan: UVAS Library [Call number: 0195,T] (1).
1
2
3
4
5
Place hold Add to cart
2.
No cover image available
Preparation And Evaluation Of A Cell Culture Contagious Pustular Dermatitis Virus Vaccine

by Shahida Afzaal | Dr. M. Ajmal | Dr. M. Akram Munir | Dr. T.M. Khan | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1990Dissertation note: The present study was conducted to prepare a cell culture vaccine against CPD to repice the older vaccine so as to induce an effective level of immunity and thus to reduce the economic losses caused by this malady. A local CPD virus strain, isolated from a direased goat during an outbreak occurred at 11.E.S. Bahadurnagar, was adapted on primary lamb kidney cells by giving several successive passages. The isolated virus was confirmed in vivo by known goat pox as well as CI']) viruses and Was used for vaccine production. Confluent primary monolayer of lamb kidney cells was achieved after 5 day of incubation. Viral material after its adaptation on kidney cells at sixth passage was inoculated on monolayer for production of vaccine. When 80% of the cells showed CPE after 6-8 days of post inoculation, the culture bottles were removed and stared at -20°C. After complete freezing, the culture bottles were thawed rapidly with vigorous shaking to disrupt the cells. The titre of the pooled virus ws found to be 10-4 in kidney cells. 5 percent suspension of the titrated virus was made in Alum Gel to prepare the vaccine. The CPD local virulent virus strain was used for challange purpose. Its titre was calculated and found to be io4.16 in susceptible goats. Sterility of the vaccine waS done on different culture media and safety was carried out in guinea pigs by inoculating the vaccine intramuscularly. The potency of the vaccine W&S done in susceptible goats( Tlilk teeth). The goats which were vaccinated in 10 ml and I ml dosage subcutaneously, and challanged after 14 days of vaccination with I ml of challange dose of 100 RD50 and 1000 RD50, proved to be immune by withstanding the above challange dose. This cell culture CPD vaccine is being used in the field with encouraging results. Availability: Items available for loan: UVAS Library [Call number: 1129,T] (1).
1
2
3
4
5
Place hold Add to cart

Implemented and Maintained by UVAS Library.
For any Suggestions/Query Contact to library or Email:rehana.kousar@uvas.edu.pk Phone:+91 99239068
Website/OPAC best viewed in Mozilla Browser in 1366X768 Resolution.