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1. A Study On The Effect Of Experimentally Induced Coccidiosis On Weight Gain And Haematological Parameters And Its Control In Qualis

by Abdul Razzaq | Dr. Asif Rabbani | Dr | Dr. Kamran Ashraf | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1999Dissertation note: The present study was conducted to see the effects of the experimentally induced coccidiosis on weight gain and some blood parameters and its control with medication and vaccination. For this purpose, 150 day-old quail chicks were purchased from the local hatchery and were reared under control managemental and coccidiosis free condition upto the 42 days of experiment. A commercial coccidiostat free feed and drinking water were provided ci libitum throughout the experiment. The birds were divided in five separate groups i.e. A, B, C, D and E, each comprising of 30 birds. Group A was kept as a non-infected, non-medicated and non- vaccinated. The chicks of groups B, C, D and E, were administered 50,000 oocysts of. tenella directly into the crop at the 18 days of age, the chicks in group C were immunized against coccidiosis with a locally prepared vaccine on 3rd and 10th day of age, while those of group D with an imported coccidiosis vaccine (Coccivac) on 7th day of age. The chicks of group E were medicated with Salinomycin on the appearance of clinical symptoms and OPG of the infected birds. No oocysts were seen in the droppings of group A chicks, throughout the experiment, while group B, chicks had the highest OPG count after induction of infection, the highest peak being in the 3rd week post-infection. The highest mortality recorded in the infected B group and low in other infected C, D and E groups and highest mortality in 3rd week of induced infection in all infected groups. The birds in groups A and E attained highest weight gain as compared with groups B, C and D but the difference was non significant. The lowest Hb and TEC values were recorded in infected group B, while in other infected groups C, D and E both these values were also decreased but comparatively lesser than B group. Similarly, values of TLC were increased in infected group B as compared with other infected groups i.e. C, D and E and the difference was statistically significant. In case of differential leukocyte counts there were increased heterophil and monocytes values in group B, while decreased values lymphocytes, basophils and eosinophils were recorded in other infected groups than the other i.e. C, D and E. The postmortem lesions observed were swollen intestines petechial haemorrhages on the wall of the intestine and dehydrated liver and lungs in infected carcasses. Availability: Items available for loan: UVAS Library [Call number: 0590,T] (1).

2. Standardization Of Avian Leukosis Diagnostic Techniques Through Polymerase Chain Reaction (Pcr) And Confirmation With Enzyme Linked Immunosorbant Assay (Elisa)

by Abdul Razzaq (M.Phil) | Prof. Dr. Zafar Iqbal Ch | Mr. Asim Aslam | Prof. Dr.Abdul Rauf Shakoori | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 2006Dissertation note: Avian Leukosis Virus type J infection of chickens is a neoplastic disease affecting chickens. ALV-J is of great economic significance not only because of tumor mortality, but also because of decreased egg production in meat breeding stocks, increased rate of infections, poor response to vaccination and weight suppression in broilers. There is wide spread prevalence of ALV-A and ALV-J in commercial chicken flocks. For control of ALV's eradication programmes based solely on dam testing may be less effective than those where dam testing is combined with procedures to mitigate early horizontal transmission in progeny chicks. For this purpose PCR along with antigen capture ELISA was used in combination for detection of ALV-J proviral DNA, and ALV group specific antigen i.e. p 27 antigen of ALV-J. Polymerase chain reaction technique was standardized by using improved version of H7 primers specific for ALV sub group J targeting env gene encoding gp85 for the detection of avian leucosis virus type J and its confirmation was carried out by comparing it with antigen capture immunosorbant assay which measures group-specific antigen (GSA) i.e. p27 antigen. Feather pulp and serum samples from 50 broiler birds of up to 7 weeks of age were randomly selected from 10 different broiler poultry farms of district Lahore Pakistan. The prevalence of ALV-J was 22 % for antigen capture immunosorbant ELISA and 34 % for polymerase chain reaction (PCR). Availability: Items available for loan: UVAS Library [Call number: 0943,T] (1).

3. Epidemological, Serological, Heamatological And Therapeutic Studies On Ovine Nematodiasis In Three Ecological Zones of Balochistan

by Abdul Razzaq | Prof. Dr. Kamran Ahraf | Prof. Dr | Prof. Dr. Azhar Maqbool.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2012Dissertation note: The main area of research in this study was to assess the prevalence, hematological and serological aspects of ovine nematodiasis. Four main experiments were conducted to highlight the objectives of the present research study. First experiment was conducted to find out the prevalence of sheep major nematodes for one year (January-December 2011). For this purpose three sheep breeds i.e., Balochi, Babrik and Harnai (either sex and between 1-5 years age groups)were selected randomly from three sites i.e., Quetta, Ziarat and Loralai. Faecal analyses of these sheep showed overall higher (40.25%) nematodes prevalence at Loralai followed by Ziarat (29%) and Quetta (23.92%). Five nematodes infection were recorded at three experimental sites. Among these, H. contortus (5.58 to 10.42%)and was the higher prevalent followed by N. battus (6.92 to 9.33%), S. papillosus (4.42 to 9%), T. colubriformis (2.33 to 7.33%) and T. ovis (1.83 to 6.83%).The nematodes prevalence was higher in one and five years old sheep. The female-sheep were infected with higher nematode prevalence higher the than male once and sometimes non-significant difference. These five nematodes were prevalent almost throughout the year; however, a peak infection was recorded during August/September. The high temperature, rainfall and humidity during these months may be predisposing factor of higher prevalence. Second experiment was on diagnosis of sheep nematodiasis through Enzyme-linked immunosorbent assay (ELISA). For this purpose H. contortus and T. ovis positive samples (200) based on coprological examination were also indicated 100% positive sensitivity by the ELISA based on crude somatic antigen, while on excretory antigen based showed lower (92%) sensitivity. The sera (n=200) of non-infected sheep (based on coprological examination) showed marked difference results. Such as 168 (84%) and 166 (83%) samples were found positive with H. contortus and T. ovis, respectively. While, based on crude somatic antigen 158 (79%) and 144 (72%) samples were found positive with H. contortus and T. ovis, respectively. Third experiment was conducted to determine the hematological values and total serum protein indices in healthy and nematodes infected sheep. The statistically significant (P<0.05) difference in PCV, Hb, RBC, WBC, Eosinophil, ESR and Total serum protein values was observed among healthy and nematode infected sheep groups. While, there was no statistically significant (P<0.05) difference in TLC, Lymphocytes, Neutrophil, Monocytes and Basophils counts in healthy and nematodes infected sheep groups. Fourth experiment was conducted on assessing the comparative efficacy of synthetic (Oxfendazole and Ivermectin) and locally manufactured herbal medicine (Deedani, Kirmar and Atreefal Deedan) anthelmintics against sheep nematodes at AZRC/PARC Range-livestock Research Station Sanjavi district Ziarat. The present study results regarding the comparative efficacy showed that, Atreefal deedan among herbal products (Deedani and Kirmar) and Ivermectin than Oxfendazole was found effective against sheep nematodes. The sheep treated with Ivermectin showed highest (96%) FEC reduction, followed by Oxfendazole/Atreefal deedan (86%), Kirmar (60%) and Deedani (32%). Availability: Items available for loan: UVAS Library [Call number: 1613,T] (1).

4. Effect Of Cholesterol Addition On Cryopreservation Of Spermatozoa And Field Fertility Of Nili Ravi Buffalo

by Abdul Razzaq | Dr. Mian Abdul Sattar | Dr. Amjad Riaz | Dr. Muhammad Avais.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1821,T] (1).

5. Isolation And Identification Of A Protease Producing Bacteria From The Decaying Plant Material And Investigation Of Its Potential As A Probiotic In Broiler

by Abdul Razzaq (2014-VA-498) | Mr. Shahid Abbas | Dr. Rahat Naseer | Dr.Muhammad Imran.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Biochemistry is gaining ground quickly due to the different preferences that it offers over customary chemical procedures particularly in regards to environment and cost contribution. Now a day’s industrial enzymes speak to the heart of biochemistry forms. Proteases are groups of organic catalysts. They have catalytic function.They hydrolyze peptide obligations of proteins and to separate them into polypeptides or free amino acids. Proteases are universal in nature and found in a wide range of differing qualities of sources, for example, plants, animals and microorganisms. Proteases do different organic procedures. Diverse types of proteases are available, alkaline proteases are a standout amongst the most imperative enzymes. The cost of the strain is a special in enzyme production and their applications in different industrial processes. The imaginative part of the present work was to distinguish and detach alkaline proteases from different organic sources, for example, from soil microorganisms with a reason to have beneficial outcome for hydrolyzing the proteins. The purified strain was checked for their potential part in chicken as a probiotics. Protease producing bacteria act as probiotic to improve digestion which results in weight gain in broiler. In this research project, bacterial strains were isolated from decaying organic plants and soil samples collected from decaying organic plants of UVAS Lahore, Pakistan. Soil and decaying samples (1.0 g) were mixed in 100 ml normal saline, afterward serially diluted from 10−1 to 10−6 ratio with normal saline. 100 μl of each diluted sample inoculated in cultural medium and incubated at 37 °C for 48 h. The isolated colonies were selected to obtain pure bacterial cultures. Cultures were maintained in nutrient agar slants. Bacilli were isolated and screened from soil samples for production of proteases. The strains were identified morphologically, biochemically and on their molecular basis. The enzyme (protease) activity was assayed according to standard procedures by slight modifications. The strain was grown and fed to chicken as probiotic. The effects of probiotic were tested for growth of chicken. The probiotics improved the intestinal microbial balance and helped broiler in gaining weight. Bacterial stain studied in the current project may be used on commercial scale for promoting or enhancing the growth of broiler chicken. Availability: Items available for loan: UVAS Library [Call number: 2717-T] (1).

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