1.
Effect Of Date Palm Pollen On The Plasma And Intra-Testicular Testosterone Levels Of Male Albino Rats
by Yasir Arfat | Dr. Muhammad Tayyab | Dr. Abu Saeed Hashmi | Dr. Ali Raza.
Material type: ; Format:
print
Publisher: 2012Dissertation note: Considerable evidence exists for the efficacy and safety of short courses of low
dose testosterone therapy for treating infertility and delayed puberty. This treatment is
associated with high levels of patient satisfaction. There is not yet sufficient evidence for
the routine use of other therapies. Experimentally, date extract had been shown to
increase sperm count and increase stimulating concentration of testosterone count in
guinea pigs and to enhance spermatogenesis, follicle stimulating hormone (FSH) and
luteinizing hormone (LH) in rats. Intratesticular testosterone (ITT) is thought to play a
key role in the control of spermatogenesis but is rarely measured.
The present study is therefore designed to examine the effect of date palm pollen
(DPP) (Phoenix dactylifera) on the plasma and intra-testicular testosterone levels using
male albino rat as an experimental animal with the hope that the result of this study may
pave the way for treating male infertility and delayed puberty.
Adult male albino rats were divided into two groups (control and experimental).
Experimental group were given date palm pollen (DPP) suspension in a single oral dose
of 120 mg/kg of body weight for 35 days. Where as the control were given equal amount
of distilled water. Blood samples of control and experimental groups were taken for
measurement of serum testosterone levels at day 0, 12, 24 and finally at day 36.Aanimals
were sacrificed. Testes were removed for gross and biological studies. Intra-testicular
testosterone levels were measured at the end of experimental studies.
There were no statistically significant differences in the variable of control group.
Experimental group who received DPP suspension for 35 days showed statistically significant increase in body weight, weight of paired testes, serum and intra- testicular testosterone levels as compared to control group.
Availability: Items available for loan: UVAS Library [Call number: 1411,T] (1).
2.
Antibacterial Activity Of Herbal Extracts Against Multi-Drug Resistant Escherichia Coli Recovered Form Retail
by Arfat | Dr. Ali Ahmad Sheikh | Prof. Dr. Masood Rabbani.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Escherichia coli is normally present in lower part of intestinal tract of all warm blooded animals. The background of drug resistant studies on E. coli represents that extensive and irrational use of antimicrobials in human and veterinary sector for treatment, prophylaxis and feed additive made this organism resistant to commonly used antimicrobials. Retail chicken meat is one of the major sources of spread of MDR E. coli infections in humans.
The purpose of present study was to evaluate the prevalence of multi drug resistant E. coli recovered from retail chicken meat samples collected from various areas of Lahore city. Also study the effect of antibacterial activity of selected herbal extracts against isolated MDR E. coli. In current study 100 E. coli isolates were processed for isolation of generic E. coli. Identification of generic E. coli was made using standard culturing, biochemical reactions and confirmed through PCR. The recovery rate of E. coli was found 80% and multidrug resistant pattern in the E. coli isolates was determined using disk diffusion method. A total 73.86% of E. coli isolates were found resistant with at least three antimicrobials related to different groups.
Current study revealed the effectiveness of herbal extracts against MDR E. coli. Clove, cinnamon and mint have good antibacterial activity as compared to coriander, kalonji and garlic. Hence, these herbal extracts can be used as promising alternatives of antimicrobials against multiple drug resistant E. coli species.
Availability: Items available for loan: UVAS Library [Call number: 1606,T] (1).
3.
Follicular Dynamics During Estrous Cycle In Sahiwal Cows
by Muhammad Yasir Arfat (2007-VA-102) | Prof. Dr. Nasim Ahmad | Dr. Amjad Riaz | Dr. Jawad Nazir | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Pakistan by default is an agricultural country. Livestock is mainstay of the farming
communities and has exclusive position in national agenda of the development. It plays an
important role in poverty alleviation and can uplift the socioeconomic condition of our rural
masses. Livestock contribution to agricultural GDP is 55.9 % and its contribution to National
GDP is 11.8 % (Anonymous,2013-2014). Total cattle population of Pakistan is 39.7 M
(Anonymous, 2013-2014). Livestock, especially cattle, play an important role in agriculture
economy of Pakistan in form of milk, meat and draught power. Milk is one of the cheapest
sources of nutrition and is beneficial for the human health in all stages of life. Despite the
nutritional importance of milk, its per capita availability and consumption is low in our country.
This inadequacy is due to suboptimal performance of indigenous dairy cattle due to lack of
modern technologies in cattle farming.
Pakistan is blessed with the finest breeds of dairy cattle such as Sahiwal, Cholistani and
Red Sindhi. Sahiwal cattle breed initially named as Montgomery breed (Bos indicus) is one of
the important breeds of indigenous cattle in Pakistan having dairy characteristics. The average
milk yield is about 1500 liters per lactation with 4% butter fat. But still its potential of milk
production is far less as compared to the exotic breeds e.g. Holstein Friesian etc. This is
primarily due to compromised feeding and management and little attention in the past for the
selection and breed improvement in Sahiwal cows. Moreover, late age at maturity and longer
calving interval (Makuza and McDaniel 1996) are major reproductive issues in Sahiwal cows.
Introduction
2
Physiology of oestrus cycle has been extensively studied in Holstein cows ( ). With the
advent of ultrasonography in early 1980’s it became possible to study follicular and luteal
dynamics during the estrous cycle in detail in Bos taurus (Fortune 1994; Lucy et al. 1992; Savio
et al. 1988; Wolfenson et al. 1995), and some beef breeds of Bos indicus cattle (Bó et al. 2003;
Figueiredo et al. 1997) and in buffalo (bubalis bubalis) as well (Warriach and Ahmad 2007).
The benefit of these studies was that the information on follicular dynamics in Bos taurus breeds
has been used to manipulate the estrous cycle in order to improve estrus synchronization
(Thatcher et al. 1993; Twagiramungu et al. 1995; Wolfenson et al. 1994) fixed time artificial
insemination (FTAI) (Pursley et al. 1997; Schmitt et al. 1996a; Schmitt et al. 1996b;
Twagiramungu et al. 1995) and embryo transfer procedure (Mapletoft et al. 1994; Roberts et al.
1994).
Sahiwal is one of the established zebu cattle (bos indicus) milk breeds of tropical and
subtropical region. It is known for its remarkable power of endurance for hot climate, resistance
to ticks and other diseases and has high producing ability under harsh environment and low cost
of maintenance as compared to the Bos indicus and Bos taurus crossbreds. Due to its promising
dairy characteristics and better adaptation to tropical environmental conditions, both the semen
and female of this breed have been exported from Pakistan and in Africa and Australia.
Differences on the reproductive characteristics between Bos taurus and few breeds of Bos
indicus cattle have been reported like luteal tissue characteristics (Pathiraja et al. 1986), Graafian
follicle (DF) diameter (Figueiredo et al. 1997) and estrous cycle duration (Castilho et al. 1996).
However, surprisingly, there has been no thorough study on the reproductive physiology of the
estrous cycle in Sahiwal cows. Therefore, the objective of the present study is to determine the
Introduction
3
follicular dynamics, luteal tissue development and regression, estrous cycle length, timing of
ovulation, estrus signs and fertility.
It is hoped that these data will be helpful for improved assisted reproductive technology
e.g. AI, ET etc. (Andrabi and Maxwell., 2007), timing of the treatment of the various hormones
(Krininger et al., 2003) and development of new technologies like fixed time A.I, estrus
synchronization, super ovulation, embryo transfer in Sahiwal cows. Ultimately, these can
increase herd reproductive, productive performance and for preservation of Sahiwal cattle breed. Availability: Items available for loan: UVAS Library [Call number: 2183,T] (1).
