1.
Comparative Efficacy Of Single And Booster Foot And Mouth Disease Vaccination In Buffaloes
by Amir, M | Dr. Atta-ur-Rehman Rizvi Chairman | Dr. Mohammad Amin Sheikh Nember | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1991Dissertation note: The present study was conducted to determine the duration of immunity conferred in buffalo-calves by locally prepared cell culture (BHK-21 cell line) FMD vaccine both in response to single dose vaccination as well as to booster dose vaccination. The cell culture bivalent FMD vaccine, having 0 and Asia-I serotypes, was prepared on BHK-21 cell line and inactivated with forma-line. The virus was adsorbed on aluminium hydroxide gel. The virus culture having the titre not less than io6 TCID50 per ml was used for preparing the vaccine. Safety, sterility and potency of the vaccine was thoroughly checked before its use in the trial.
Eighteen buffalo-calves, aged 1-2 years, were used in the experiment. Their freeness from FMD and susceptibility were tested by performing the serum neutralization test in cell culture, which indicated the absence of neutralizing antibodies in their sera against the vaccine virus types. Their faecal examination was negative for any internal parasite after deworming twice. Twelve buffalo-calves were vaccinated subcutaneously with the field dose i.e. 5 ml and 6 animals were re-vaccinated on 28th day of primary vaccination while other 6 buffalo-calves were left as unvaccinated control. The vaccinated animals showed slightly rise in temperature 'and developed a walnut size swellings at the sites of injection otherwise they looked to be healthy.
Serum samples were collected at 0 day to 26th week from once vaccinated animals and upto 36th week from the booster vaccinated and control animals. Almost all the vaccinated animals developed antibodies against FMD on 1st week, but all the vaccinated animals developed protective levels of neutralizing antibodies against FMD on 2nd week of primary vaccination. The single dose vaccinated buffalo-calves maintained the protective level of antibodies upto 16 weeks while booster vaccinated animals remained protective for 24 weeks against both the vaccine virus types (0 and Asia-I), to which booster vaccination was done on 28th day of primary vaccination.
It was concluded from this study that the buffalo-calves of this age group should be re-vaccinated after 18 weeks of primary vaccination to protect them for further 24 weeks against F'MD i.e. booster vaccination is must for scheduling at least the biannual vaccination. Subsequent re-vaccination will increase the time span for repeating the vaccination.
Availability: Items available for loan: UVAS Library [Call number: 0355,T] (1).
2.
Effect Of Maternal Antibodies On Antigenic Response Of Broiler Birds To Infectious Bursal Disease (228-E strain) Virus Vaccine
by Sajid Afzal, M | Dr. Muhammad Akram Munir | Dr. Atta-ur Rehman Rizvi | Dr. Haji Ahmad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1997Dissertation note: This project was designed to study the effect of maternal antibodies on the antigenic response of birds to infectious bursal disease virus (288-E strain) vaccine and to study the duration of maternal antibody against IBDV in broiler. A total of one hundred forty chicks were randomly divided into eight groups i.e. A, B, C, 1), E, F, G and H. All the birds in groups A, B, C, U, E and F were vaccinated against IBDV. The birds in groups 0 and H were unvaccinated controls. However, the groups were IBDV vaccinated according to different schedules.
The presence of IBDV maternal antibodies was tested through agar gel precipitation test, and the maternal antibodies to IBDV were observable upto day 14 of the life of chicks.
All the IBDV vaccinated birds exhibited immune response to the vaccine and their response was detectable through AGPT. Antibodies were detectable on 7th day post vaccination and the responses increased upto 14th day post vaccination. The antibody titres were at peak in all the groups between 28th to 35th day of age.
These findings indicate that maternal antibody waned away between second and third week of life. The mean±standard error values of groups A, B, C, D, E, F, G and H on day 35, were 28.33±8.56, 41.66±12.99, 43.33±11.70, 31.66±13.21, 41.66±12.56, 45.0±14.29, 26.66±11.94 and 26.66±11.94, respectively. The serologic and challenge infection study indicates that the vaccinated birds were immune against IBDV.
Availability: Items available for loan: UVAS Library [Call number: 0515,T] (1).
