Your search returned 4 results. Subscribe to this search

Not what you expected? Check for suggestions
|
1. Effect Of Cholesterol Loaded Cyclodextrin (Clc) Addition On Post Thaw Quality Of Jack Semen

by Muhammad Rafi Ullah (2009-VA-54) | Dr. Mushtaq Ahmad | Dr. Muhammad Zahid Tahir | Dr. Muhammad Hassan Saleem.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Improvement of post-thaw quality of Donkey (Equusasinus) semen is essential to augment the in-vivo and in-vitro fertilization rate. Whenever we think about the techniques for long term storage of the germ cells cryopreservation appears most useful but it causes lethal and sub-lethal damage to the sperm. Sperm cryosurvival rates are not optimal for most species including donkey because of its plasma membrane composition. Egg yolk is an important component of most of the equine freezing extenders. But another factor regarding this important component is that its higher concentrations can have some deleterious effects such as oxidase activity of dead spermatozoa, bacterial/ xenobiotic contamination, reduced respiration and motility of the spermatozoa, which ultimately results in lowering fertility rate. That’s why, cold shock resistance is more in those species semen possessing higher membrane cholesterol to phospholipids ratio as compare to those species semen having less cholesterol to phospholipids ratio. Indeed it may be a useful strategy to improve cryopreservation protocols for jack sperm. Addition of CLC may also be a substitute for egg yolk in semen extender always considering that the cryo-protective effect of EY is partially due to its high cholesterol content. Semen was collected in artificial vagina at 42ºC from two mature donkeys (n=2) five times (replicates=10) which were maintained at Ravi campus Pattoki. Semen samples possessing >60% motility were used in this study. Each ejaculate was divided into 6 aliquots and CLC was added into these aliquots according to desired concentrations (0 mg, 1.5 mg and 3 mg/120 million sperms) then kept at 25ºC for 15 minutes. After incubation semen aliquots were diluted in 1:1with centrifugation media and centrifuged at 600g for 15 minutes to separate seminal plasma after bead formation. Beads of the semen were resuspended in the centrifugation media having different concentrations of egg yolk for different CLC concentration groups in such a way that 100 million / ml sperms concentration was achieved. When this final dilution was done semen was shifted at 4 ºC for 2 hour CHAPTER 6 SUMMARY Summary 37 cooling then further 2 hours for equilibration at 4º C. Then semen was packed in 0.5 ml plastic straws. These straws were suspended on liquid nitrogen vapors upto7 mints then plunging these straws in the liquid nitrogen in freezing box and then shifting these straws to the liquid nitrogen container by placing them in the goblets and stored there until post thawing was done. After post thawing semen was analyzed for motility, Acrosomal and plasma membrane integrity, live ratios DNA integrity and MDA levels. Post-thaw parameters were analyzed by PROC MIXED as factorial ANOVA using SAS enterprise Guide Version 4.2. And it was observed that. And it was observed that both CLC 1.5 and 3 mg/120 million sperms with full egg yolk did not improved (p˃0.05) the post thaw quality except in malondialdehyde levels in which 3 mg dose with full yolk significantly (p˂0.05) decreased the level of malondialdehyde. While CLC with lower levels of egg yolk maintained the quality same as the control or improved significantly in some parameters.CLC1.5 HEY and CLC3 NEY both compete the control in motility, PMI by HOST and total viability parameter..DNA integrity increased with the decrement of egg yolk as CLC1.5 HEY, CLC3 HEY and CLC3 NEY are significantly (p˂0.05) better than the remaining three groups.MDA level is significantly lower in CLC1.5 HEY and CLC3 HEY groups comparable to control means that with partial removal of egg yolk and supplementation of CLC malondialdehyde levels are decreased significantly as compare to control. Thus CLC substitutes the egg yolk completely when 3mg of CLC /120 million sperms is added in kenney’s extender for jack semen cryopreservation. Availability: Items available for loan: UVAS Library [Call number: 2553-T] (1).

2. Treatment Of Long Oblique Tibial Fracture With Intramedullary Pin Alone And Full Cerclage Wires In Cats

by Syed Muhammad Faheem Ahmed (2010-VA-147) | Dr. Ayesha Safdar | Prof. Dr. Muhammad Arif Khan | Dr. Muhammad Zahid Tahir.

Material type: book Book; Literary form: not fiction Publisher: 2017Dissertation note: Fracture of long bone in animals is a challenging problem these days. Leg fractures in cats are quite common problem. Fractures treated with biomechanically sound fixation and proper attention to soft tissues will be most likely to heal with a functional outcome. However, osteomyelitis, bone sequestration, and joint ankylosis still continue to be major factors in inhibiting healing and bone functionality. This research project was designed with the aim to test the effectiveness and accuracy of three sets of bone splintage devices in healing long oblique mid-shaft tibial fractures in cats, with minimal side effects and stress to the patient. For the study total twelve adult cats as and when presented at Pet Centre, University of Veterinary & Animal Sciences Lahore, and Saleem Veterinary Clinic and petshop Lahore, having long oblique fracture of tibia irrespective of breed, sex and considering almost similar age group, both stray and domesticated were selected. These cats were divided into three groups i.e groups A, B and C comprising of four cats in each group, and designated as 1, 2 3 and 4 respectively. They were admitted as clinical trial patients and were kept in separate cat cages at Saleem Veterinary Clinic until the completion of the study. Specific tagged collars were applied on all the cats. They were kept under comfortable environment and on a standard commercially available cat feed diet with ad labitum access to water. All the cats of three groups, having long oblique tibial fracture were treated with 3 separate techniques for comparison. In group A, fracture was reduced and immobilized with intramedullary pinning. In group B, fracture was treated with full cerclage wires alone while cats of group C were treated with intramedullary pin and cerclage wires. Thus comparative fracture healing and efficacy of individual techniques in their respective cases were evaluated. Subsequent comparative parameters were studied which included physical examination of wound, lameness grading, radiological scoring for callus formation, fracture line union, fracture alignment as well as callus remodelling. The collected data regarding study parameters were illustrated by descriptive statistics and non-parametric analysis of variance (Kruskal Walis Test) using IBM SPSS Statistics version 20. Conclusion: Therefore, as advocated by the results of this study, intramedullary pinning (preferably threaded) with at least 2 - 3 full cerclage wiring was a better choice for the correction of a long oblique mid-shaft tibial fracture in cats. The combination of both these techniques together give a strong backbone for bone healing to take place with minimum amount of stress to the bone fragments and also to counteract different forces acting on the fracture site.Both these techniques were easy, required little soft tissue manipulation, were cost-effective, required less equipment and could easily be applied by an average practitioner. Availability: Items available for loan: UVAS Library [Call number: 2891-T] (1).

3. Qualitative And Quantitative Assessment Of Fertile Period In Bitches

by Anam Mushtaq (2009-VA-388) | Dr. Muhammad Zahid Tahir | Dr. Amjad Riaz | Dr. Muhammad Hassan Saleem.

Material type: book Book; Literary form: not fiction Publisher: 2017Dissertation note: The estrous cycle of the bitch consists of 4 phases including proestrus, estrus, diestrus and an obligatory anestrus. Most of the owners normally assess the fertile period on the base of qualitative parameters and mate their bitches on predetermined days. This is the major cause of apparent infertility in bitches. The most reliable assessment for timing of mating is through quantitative assessment through vaginal cytology and progesterone assay. The objective of this study was to compare the outcomes of qualitative and quantitative assessment of fertile period in bitches. Furthermore, the effect of assessment method was studied on fertility (number of pregnant bitches) and prolificity (number of pups in a litter). This study was conducted on 148 referral cases of bitches brought to Theriogenology Laboratory. The data of qualitative assessment by the owners was collected by a questioner while quantitative assessment was based on vaginal cytology and progesterone assay. Statistical analysis was done using SPSS and logistic regression to compare the fertility (number of pregnancies) and prolificacy (number of pups) among groups. In qualitative group, only 32% bitches were diagnosed pregnant. In contrast, the quantitative group showed a significantly higher fertility (83%) as compared to qualitative group (P<0.05) with odd ratio of 3 times higher fertility. Similarly, in qualitative group, 58% bitches had litter of 1-4 pups while the rest had a litter ranging in 5-10 pups. In contrast, quantitative group showed significantly (P<0.05) higher prolificity with 79% bitches having a litter of 5-10 with 4 times higher odd ratio. Meanwhile, the percentage of bitches having a smaller litter (1-4 pups) was considerably low (21%). In conclusion, the quantitative assessment of fertile period is more accurate and results in significantly higher fertility and prolificity. Availability: Items available for loan: UVAS Library [Call number: 2946-T] (1).

4. Effect Of Antibiotic Treatment During Ovulation Synchronization In Repeat Breeder Holstein Friesian Cows

by Masood Shabbir (2010-VA-120) | Dr. Muhammad Zahid Tahir | Dr. Amjad Riaz | Dr. Muhammad Avais.

Material type: book Book; Literary form: not fiction Publisher: 2017Dissertation note: Repeat breeding is one of the major causes of reproductive, productive and economic losses in dairy sector. The main causes of repeat breeding include sub-clinical infection of reproductive tract, age of the animal, error in the detection of estrus, endocrine dysfunction and nutritional deficiencies. In Pakistan, a very high incidence of repeat breeding has been documented (Kakar et al. 1997). In the past, intrauterine infusions have successfully been used with a variety of antiseptic and antibiotic solutions. Meanwhile, therapeutic use of GnRH and PGF2α has also been demonstrated to result in improved pregnancy rates. In particular, Ovsynch protocol leads to an increase of 6-17% in conception rate. The objective of the present study was to evaluate the reproductive efficiency with antibiotic treatment during ovulation synchronization in repeat breeder Holstein Friesian cows. This study was conducted on 30 pure bred Holstein Friesian cows kept under standard farm conditions of feeding and management at Hussain Cattle and Dairy Farm, Kasur. The reproductive efficiency of treated and control animals was based on ovulation rate, non-return rate, conception rate, pregnancy rate, embryonic losses and luteal function. Results were analyzed by independent T-test. A probability level of 95% was consider as significant (P<0.05). All the experimental animals were screened to confirm non-pregnant and normal genitalia. Both the treatment and control groups were synchronized using ovsynch protocol. Following first injection of GnRH, the treatment group was subjected to intrauterine antibiotic infusion for five days. On Day 7 of protocol both groups received an injection of PGF2α. At day 9 of ovsynch protocol before second injection of GnRH both groups were scanned for ovarian status. Follicles and CL measurement were noted and mapped by using 7.5 MHz trans-rectal probe (Honda 22 Summary Model HS-1600) in both groups and second injection of GnRH was given. Timed artificial insemination was performed after 16-20 hours of second injection of GnRH in both groups. After 8 hours of artificial insemination again ultrasonography was conducted to check the ovulation rate in both groups, there was no significant difference between control and treatment, 60% and 75% of the animals in control and treatment groups ovulate respectively. At day 11 blood samples were collected for progesterone assay. Non-return rate was visually observed at day 18 to 21 of artificial insemination. Blood was collected at day 18 for progesterone assay by puncture of tail vein. After D28 andD42 of artificial insemination first and second pregnancy test were conducted by using 7.5 MHz trans-rectal probe (Honda Model HS-1600) in control and treatment group. There was a significant difference of pregnancy among the control and treatment group by independent T-test. 3 rd and 4 th blood samples were collected for progesterone assay at day28 and 42 of artificial insemination in both groups respectively. Availability: Items available for loan: UVAS Library [Call number: 2951-T] (1).



Implemented and Maintained by UVAS Library.
For any Suggestions/Query Contact to library or Email:rehana.kousar@uvas.edu.pk Phone:+91 99239068
Website/OPAC best viewed in Mozilla Browser in 1366X768 Resolution.