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1. Physico-Chemical And Sensory Characteristics Of Feta Cheese Made From Sheep Milk Blends.

by Muhammad Adeel | Dr. Imran Javed | Dr. Muhammad Nasir | Dr. Saima Inayat.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1639,T] (1).

2. The Effect Of Date Palm Fruit (Phoenix Dactylifera L.) Extract On Oxidative Stabilization Of Butter At Ambient

by Fazal Rehman | Dr. Muhammad Nadeem | Dr. Saima Inayat | Dr. Yasir Zahoor.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2013Dissertation note: This investigation aimed to explore the antioxidant potential of date fruit extract using butter as an oxidation substrate on the basis of certain instrumental, orthodox analytical techniques and sensory characteristics. The objectives of this study were to characterize the date extract for polyphenolic compounds, free radical scavenging activity and inhibition of nitric oxide macromolecules and to study the effect of various concentrations of the date palm extract on oxidative stability and sensory characteristics. The butter was added 200, 400 and 600-ppm respectively date palm extract and compared with a control, stored at refrigeration and ambient temperature during winter months for 90-days at the interval of 30-days. Peroxide value, anisidine value, iodine value, conjugated dines, totox value, free fatty acids and sensory evaluation were performed in the fresh and three month stored butters. Sensory evaluation of supplemented butter added with various concentrations of date palm extract stored revealed the effect of treatments, storage period and their interaction to be significant. Taste, Color and overall acceptability score was non-significant up to 30-days of storage. After this sensory score started to deteriorate and went on decreasing the rest of storage period. The decline in taste score was not due to the addition of date palm extract rather it was due to the generation of peroxides and other oxidation products. Oxidation products and peroxides are found to be in direct relation with temperature; with rise in temperature the decline in sensory evaluations was more pronounced. This connection between taste score and peroxide value was highly correlated (R= 0.9982). The results of triplicate treatments and triplicate analysis (n=3x3; ±SD n=3x3) was analyzed by using one way analysis of variance techniques (ANOVA) to find out the effect of the date palm extract to the storage conditions. Differences among treatments were compared by Duncan Multiple Range Test. Supplementation of butters with date palm fruit extract did not revealed any significant effect on the inhibition of free fatty acids (P>0.05). The free fatty acid content of fresh and 90 days stored treatments and control were at par with each other. The peroxide value of all the treatments and control increased during the storage period but to varying degree. The addition of date palm extract revealed a significant effect on inhibition of peroxide value (P<0.05). The peroxide value of fresh supplemented, control and 90 days stored samples showed a significant difference. The difference in control and treatments were in order of To > T 1 > T2 > T3 in 90-days stored experimental samples and control. T3 revealed a maximum resistance towards the increase of peroxide value during storage period of 90-days over the control at refrigeration temperature. It is evident from the result that the addition of date palm extract significantly inhibited the auto-oxidation phenomenon in butter stored at room temperature, however the concentration of oxidation products were higher as compared to butter stored at refrigeration temperature. The iodine value decreased during the storage period in control experimental samples throughout the storage period of 90-days but to varying extents at refrigeration temperature. Control (T0) showed highest decrease in iodine value while T3 showed least change in iodine value during the storage period. The anisidine value of control and treatments stored at refrigeration temperature were in the order T0 > T1 > T2 > T3. Control revealed significant increase in anisidine value as compared to treatments. After 90 days of storage period the extent of secondary oxidation products in control and T3 was 22.24 and 8.35 at refrigeration temperature respectively. The classical rise of anisidine value was observed in all the treatments to varying degree, supplementation of natural antioxidant offered a great deal of resistance towards the rise of anisidine value. Supplementation of butter with date palm fruit extract revealed significant effect on the generation of oxidation products. Conjugated dienes for control and all the treatments increased throughout the storage time slowly and steadily. Analysis of variance revealed that the effect of treatments, storage period and their interaction was significant. Taste, Color and overall acceptability score was non-significant up to 30-days of storage. After this sensory score started to deteriorate and went on decreasing the rest of storage period. The results indicate that ethanolic date palm extract at 600-ppm concentration can be used as natural antioxidant source for the long term storage of butter at ambient temperature during winter months. The outcome of this study will help the food industry for preservation of fats and oils. Availability: Items available for loan: UVAS Library [Call number: 1800,T] (1).

3. Quality Enhancement Of Soy Milk And Soy Yoghurt Blender With Buffalo Milk

by Muhammad Asad Hameed | Dr. Saima Inayat | Dr. Imran Javed | Dr. Muhammad Nasir.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Yoghurt is a fermented milk product, which is produced by the bacterial fermentation of milk. It is a rich source of calcium, protein and vitamin B-complex. Lactose-intolerant people can eat yoghurt without any harm as lactose is converted into lactic acid by the bacterial culture. Yoghurt is more nutritive then milk and possesses better digestibility. The benefit of yoghurt depends upon the presence of beneficial viable bacterial culture in adequate number. The bacterial cultures are used in the fermentation process to metabolize the lactose, secondly the proteolysis of protein for improving bioavailability and thirdly lactic acid bacteria for production of some B-complex vitamins and vitamin K. Yoghurt culture are responsible for the production of aromatic flavor compounds. In Pakistan the production of soy milk and soy yoghurt isnot common because ofconsumerunawareness for such kind of products. Therefore, this study was designed to develop soy milk and soy yoghurt by using different concentrations of buffalo milk. To produce soy yoghurt, soy milk and whole milk was pasteurized at 85°C for 30 minutes to kill the pathogenic microorganism. Thencooled at 42°C. This milk was used for the production of yoghurt.Thecommercial starter culture was used for the manufacturing of soy yoghurt. This culture was imported from Italy. During storage the prepared soy yoghurt was evaluated for its sensory qualities. A panel of judges evaluated the yoghurt samples for appearance, taste, color and overall acceptability on 9-point hedonic scale (9 = like extremely; 1 = dislike extremely). The sensory evaluation of the product at 0, 7, 14 and 21 day was carried out in the Department of Dairy Technology, University of Veterinary and Animal Sciences, Ravi Campus, Pattoki. There was significant effect of storage on sensory attributes of soy yoghurt. Highest score was awarded to T0. Soy yoghurts were evaluated for physico-chemical parameters (Fat, protein, total solid, acidity, pH and ash) during storage of 21 days with 7 days interval. Fat, pH and ash contents were continuously decreased while protein, total solid and acidity values show continuous increase in all treatments. Soy yoghurt was also evaluated for microbiological examination (total plate). Highest microbial count was observed in T0. Availability: Items available for loan: UVAS Library [Call number: 1842,T] (1).

4. Qualitative Studies Of Cheese Made By Using Bifidobacterium Bifidum & Lactobacillus Acidophilus

by Abid hussain | Dr. Saima inayat | Dr. Jalees | Dr. Muhammad ayaz.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2042,T] (1).

5. Determination Of Heavy Metals In Cauliflower And Carrot Grown In Suburbs Of Lahore

by Hafiz Muhammad Azam Arif (2013-VA-849) | Dr. Zubair Farooq | Dr. Sana ullah Iqbal | Dr. Saima Inayat.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Vegetables are the fresh and edible portions of the herbaceous plants. They classified as seasonal vegetables but they also classified as root, tuber, shoot, flower, fruit and leafy on the base of mode of consumption. Each of the categories mentioned above have its own role in human body. They play a vital role in human health due to their higher nutritional values. They are major source of macro and micro nutrients which required by the human body for optimal functioning. They are not only involved for normal body functioning but they also disease preventive in nature. Heavy metals get entry through different routes and accumulate in edible and inedible portions of the vegetables in quantity enough to cause clinical problems to humans and animals. This study was designed to assess the level of heavy metals in vegetables in different districts of Punjab. Heavy metal is defined as metallic element usually toxic in nature with high density and atomic weight. As they are toxic in nature they accumulate in different parts of the vegetables and lead to several health complications in humans. Some of these metals are of nutritionally important for human health in small quantities and referred as trace elements which include zinc, iron, copper etc. They are among the major toxicants in vegetables which may cause chronic diseases in the kidney and liver of humans and causes disruption of numerous biochemical processes leading to cardiovascular, nervous, kidney and bone diseases. Open crown vegetables like cauliflower have more chances of infestation in suburbs due to waste water irrigation as compared to intact skin vegetables especially grown away from suburbs. Moreover, vegetables treated under clean sanitary conditions can have low levels of heavy metals as compared to the untreated ones. There are different sources of heavy metals which include waste water irrigation, industrial waste water and soil pollution which associated with industrialization and agricultural activities such as atmospheric deposition, waste disposal, waste incineration, vehicle exhaust, fertilizer application and long term application of sewage sludge in agriculture land. Waste water contains sufficient amount toxic heavy metals which cause clinical problems. In the present study two vegetable cauliflower (Brassica oleracea) and carrot (Dascusorrota) were collected from suburbs of Lahore. While samples of cauliflower were include from various districts of Punjab. The heavy metals Pb, Zn, Cr, Ni, Ca, Mg, K and Na were determined in cauliflower and carrot from suburbs; While Pb, Zn, Cr and Ni were observed in cauliflower from various districts of Punjab. Digested vegetable samples were analyzed through Atomic Absorption Spectrophotometer. The highest levels for heavy metals (ppm) Lead, Zinc, Chromium and Nickel in Lahore for cauliflower and carrot was (2.2267±0.0251, 2.2317±0.0076), (5.7100±0.0360,5.0333±0.2516), (1.1133±0.1154, 1.7333±0.0763), (2.466±0.1040, 2.4000±0.3605) respectively. There was a significant difference in both vegetables. Difference in concentration in both vegetables is due to the surface area of both vegetables as cauliflower has large surface area as compared to carrot. The highest levels for heavy metals (ppm) Lead, Zinc, Chromium and Nickel in Gujranwala for cauliflower was (2.3266±0.0145, 4.5966±0.0152, 0.9333±0.0152, 2.2467±0.0251) respectively. The highest levels for heavy metals (ppm) Lead, Zinc, Chromium and Nickel in Sheikhupura for cauliflower was (1.9000±0.0200, 6.043±0.0513, 0.933±0.0152, 2.490±0.0100) respectively. The highest levels for heavy metals (ppm) Lead, Zinc, Chromium and Nickel in Faisalabad for cauliflower was (1.928±0.0104, 5.736±0.0156, 1.193±0.0057, 2.476±0.152) respectively. The highest levels for heavy metals (ppm) Lead, Zinc, Chromium and Nickel in Kasoor for cauliflower was (1.900±0.0200, 6.043±0.0513, 0.933±0.0152, 2.490±0.0100) respectively. Each of the city have different concentration of the metals, several reasons for this variation which include medium on which these vegetables are growing, water used for irrigation, application of agricultural products, poor agricultural practices and post-harvest treatment which include harvesting procedure, washing of vegetables with unsafe water and transfer from long rooted areas. Uptake behavior of these metals and minerals by vegetables also affect it. The transfer factor (TF) of different heavy metals from soil to vegetation is one of the key components of human exposure to metals through the food chain. The highest TF valueis found for Zn because Zn is more mobile in nature. This study will provide a baseline data and there is a need for intensive sampling of the same for quantification of the results. Soil, plant and water quality monitoring, together with the prevention of metals entering the plant, is a prerequisite in order to prevent potential health hazards of wastewater irrigation. By using good agricultural practices including use of less agricultural chemical products and sewage water should be used after proper treatment. Availability: Items available for loan: UVAS Library [Call number: 2454-T] (1).

6. Development And Quality Enhancement Of Cottage Cheese Made From Nili Ravi Buffalo Postpartum Milk (Colostrum)

by Maryam Batool (2010-Va-360) | Dr. Saima Inayat | Dr. Muhammad Ayaz | Dr. Saeed Ahmad.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Colostrum is milk obtained from mammals secreted after parturition. It differs from normal milk as it contains numerous bioactive components which include growth factors, lacto-peroxidase, lacto-ferrin, Igs, lysozyme, nucleosides, vitamins, oligosaccharides and peptides, which are health promoters. Cottage cheese is an excellent source of cyanocobalamin, riboflavin and pyridoxine. Using colostrum for making cottage cheese can give opportunities to increase cottage cheese quality which may be more functional, healthier with high yield. Preservation of colostrum in the form of cottage cheese can be supportive for obtaining functional cheese with enhanced shelf life. Nili Ravi Buffalo postpartum milk was used for the production of Cottage cheese. While the control was prepared from Nili-Ravi buffalo normal milk. Milk was pasteurized at 63 °C for around 30 minutes in batch pasteurizer. Then the milk temperature was brought down at 37°C for inoculation with the mother cultures mentioned above at 2% level. Three replicates for each treatment was prepared. Incubation continued for 30 minutes at 42°C. Then 1.7 ml CaCl2 was added and after 10 minutes 1.7 ml of rennet solution was added in cheese milk and left for another 40 minutes for coagulation. Then the curds was cut into 1cm3 and healed for 10 minutes. The temperature is raised to 55°C for cooking for 30 minutes. Then whey was drained for 24 hrs. The curd was pressed and shaped into mold and then wrapped in aluminum foil and stored at 4°C for 7 days for further analysis. The physicochemical analysis such as pH, Acidity%, Fat %, TS %, Ash %, Chlorides %, Casein %, Protein% and Lactose% of Colostrum and Colostrum based Cottage cheese with respect to control was carried out by standard methods as described in AOAC. The data thus collected were analyzed through one way analysis of variance under Completely Summary 139 Randomized Design (CRD). Means were compared through Duncan’s Multiple Range (DMR) Test (SAS 9.1 Statistical Software). Physicochemical analysis of Colostrum showed that pH is in the increasing trend with the increase of age of milk and results showed that Colostrum pH values lower than milk values and were related to the high proteins content that is usually revealed in colostrum obtained 2-3 days after birth. While the trend of titratable acidity %, fat %, chlorides %, specific gravity, ash %, Total solids %, Total protein % and casein % is decreasing with an increase in the age (Days) of postpartum milk. Physicochemical analysis of Colostrum Cottage Cheese showed that the trend of pH of colostrum cottage cheese remained constant with the increase of age. The trend of lactose% and ash % is increasing with an increase in the age of postpartum milk. While the trend of titratable acidity %, fat %, chloride%, total solids %, total protein % and casein % of cottage cheese is decreasing with an increase in the age (days) of postpartum milk. Microbiological analysis of Cottage cheese was examined. Coliforms remained absent (<10 cfu/g) during whole storage time. Total Plate Count (TPC) was not significantly different throughout the storage period as it renders growth if there are hygiene and proper storage conditions available. Sensory evaluation was be carried out using criteria of appearance, taste, color, flavor and whole acceptability on a hedonic scale of nine points. T0, T3, T4 and T5 showed cheese like aroma, T1 showed yoghurt like aroma while T2 showed cheese like/ slight acidic aroma after 7 days of storage period. T0 and T5 showed whitish appearance, T1 showed light yellow appearance, T2 showed slight creamy like color after 7 days of storage period while T3 and T4 showed off white appearance after 7 days of storage period. T0, T4 and T5 showed light acidic flavor, T1 and T2 Summary 140 showed slight saltish / most bitterness in taste while T3 showed acidic taste after 7 days of storage period. T0 and T5showed granular texture with no creaminess, T1 showed soft yoghurt like texture, T2 showed meaty / hard texture while T3 and T4 showed harder toffee like texture after 7 days of storage period. Colostrum is the most proteinaceous food as compared to milk and improves the nutritional aspects as well as quality and yield of the products. So Cottage cheese made from Colostrum is more nutritious, functional with probiotic characters as well as enhanced physico-chemical properties with high yield. Availability: Items available for loan: UVAS Library [Call number: 2473-T] (1).

7. Quality Enhancement Of Buffalo Milk Yoghurt By Using Glutathione Treated Transglutaminase Enzyme As A Gelatin Replacer

by H. M. Arslan Amin (2009-VA-486) | Dr. Saima Inayat | Dr. Muhammad Junaid | Prof. Dr. Khalid Javed.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Yoghurt is a fermented dairy product and intensively used in all over the world. It is very popular in sub-continental region due to its high consumption as compare to other regions of the world. Fermentation of milk is carried out by mixture of lactic acid bacteria. Synergistic effect of Streptococcus thermophillus and Lactobacillus delbrueckii ssp. bulgaricus is a key factor which determines the rate of fermentation process and quality of yoghurt. In traditionally made set yoghurt, protein gel is stabilized by weak non covalent interactions (hydrogen bonding, electrostatic interactions and hydrophobic bonds). Addition of Transglutaminase enzyme (TGase) can provide opportunities to increase covalent bonding and cohesiveness which ultimately enhances the gel consistency with reduction of syneresis. For the production of yogurt standardized buffalo milk of 5.5% fat and 9.5% SNF contents were used. Milk was pasteurized for 15 seconds at 72°C to kill the pathogenic bacteria and cooled to 42°C. After cooling the Glutathione treated Transglutaminase enzyme was added in the milk with different concentrations (1.25g/250ml, 2.50g/250ml, 3.75g/250ml and 5g/250ml) and cooled to 42°C. While the control sample was prepared without the addition of Glutathione treated Transglutaminase enzyme. After that each treatment was inoculated with standard yoghurt culture (Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus) at the rate of 2% at 42°C for 3-4 hours and each sample was stored at 4°C in a refrigerator for 28 days. The physico-chemical parameters such as pH, acidity %, fat %, protein % and syneresis % were observed weekly till 4 weeks. The quality comparison of prepared yoghurt was carried out by using Two Way Analysis of Variance (ANOVA) technique under Completely Randomized Design. Significant means were compared using Duncan’s Multiple Range (DMR) Test with the help of SAS 9.1. Results showed variations in pH, acidity %, fat%, protein %, snf% and syneresis % values due to the production of Yoghurt with Glutathione treated Transglutaminase in different percentages. pH in different treatments decreased from 4.71 – 4.61. Acidity % during storage period increased from 0.40 % to 0.43 %. Fat % also showed some variation during storage period decreased from 5.37% – 4.49 %. Protein % in different treatments increased from 4.10 % – 4.27 % and SNF % in different treatments increased from 9.81% – 10.00 % . Syneresis % in different treatments decreased from 5.17% - 3.48 %. Sensory evaluation was carried out by a panel of 06 semi trained people, using criteria of appearance, taste, color, flavor and whole acceptability on a 9 points hedonic scale. Color, taste, aroma, acidity, appearance and overall acceptability showed non-significant results as compared with controlled sample after 28 days. Results of the present study showed that yoghurt prepared with Glutathione treated Transglutaminase enzyme reduced the syneresis and enhanced the nutritional aspects of yoghurt. Availability: Items available for loan: UVAS Library [Call number: 2483-T] (1).

8. Enhancement Of Omega Fatty Acids In Margarine Through Chia (Salvia Hispanica L.) Oil

by Hafiza Anam Baig (2010-VA-355) | Dr. Muhammad Nadeem | Dr. Saima Inayat | Dr. Muhammad Hayat Jaspal.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: The higher incidents of deaths from cardiovascular diseases have led to the identification and modification of risk factors for atherogenesis; it is regarded as the number one reason of deaths in developed and underdeveloped countries. Chia contains about 40% edible oil, with fairly high content of unsaturated fatty acids, oil contains about 60% omega-3 fatty acids on weight basis. Margarine will be prepared from palm oil, palm kernel oil, butter and chia oil blends by varying the concentration from 5% to 20%. Margarine was prepared by blending butter, palm oil and Chia seed oil in various proportions. Market margarine (Blue Band) was used as a positive control. Butter, Palm oil and Chia Seed oil was characterized for free fatty acids, moisture content, saponification value, unsaponifiable matter, iodine value, refractive index. Solid fat index was determined at 0, 10, 20, 30 and 40˚C by the dilatometric method, melting point was determined by open capillary tube technique. Colour, free fatty acids, iodine value, peroxide value, anisidine value, conjugated dienes and trienes was determined. Fatty acids composition of margarines was determined by the transformation into fatty acid methyl esters. Margarine was stored at 6±1oC, for 2 months, iodine value, peroxide anisidine values, free fatty acids, conjugated dienes and trienes was determined. Sensory evaluation was performed by a panel of ten trained panel of judges, samples was evaluated for color, smell, taste and overall acceptability on a 9-point Hedonic scale. Peroxide value of 60 days old control and T4 (12% chia oil) were 7.06 and 12.10 (MeqO2/kg). After 60 days of storage period, peroxide value was in the order of T4> T3> T2> T1> control. Free fatty acids increased with the storage period in all the treatments. They are also increasing with the treatments having higher concentration of chia oil. At 60 day, control Summary 68 and T4 are 0.16 and 0.19% respectively. Anisidine value trend is considerably accelerating along the treatments as well as with the storage time. At 60 day, control and T4 is 24.42 and 44.78 respectively. Conjugated Dienes have the increasing trend throughout the treatments. They also have higher values from 0 to 60 days of time period. At 6o day, the control and T4 are 2.47 and 3.34 respectively. Conjugated Trienes of all the samples increased throughout the storage period of 60 days. At 60 day, the control and T4 are 2.60 and 3.28 respectively. Iodine value is in the decreasing trend throughout the storage period and the treatments. They are in the order of T0>T1>T2>T3>T4. Fat content of all the treatments and the control went on decreasing throughout the storage period of 60 days. At 60 day, control and T4 were 68.61 and 90.91 respectively. The order of the fat content in all the treatments were T0>T1>T2>T3>T4. Moisture content of all the treatments and the control went on decreasing throughout the storage period of 60 days. At 0 day, Moisture content of all the treatments were virtually the same. At 60 day, control and T4 were 27.52 and 7.64 respectively. Salt content of all the treatments at the end of the storage period were increased. At 60 day, control and T4 were 4.18 and 1.42 respectively. The taste score of all the treatments along the treatments having different concentrations of unsaturated fatty acids and also along with the storage period of time is decreased. The taste score of the control and T4 were 8.06 and 6.90 respectively. The order of the taste score is in the descending order of T0>T1>T2>T3>T4. Results of this evidenced that concentration of omega fatty acids can be enhanced in margarine through chia oil with acceptable sensory characteristics. Major changes in fatty acid composition were observed around C18:2 and C18:3 (omega 6 and 3). Concentration of trans fatty acids in control, T1, T2, T3 and T4 was 24.75%, 6.67%, 5.49%, 4.71% and 3.19%. Availability: Items available for loan: UVAS Library [Call number: 2578-T] (1).

9. Effect Of Inulin Present In Chicory Roots On The Diabetes

by Saeed Ahmad (2009-VA-642) | Dr. Sana Ullah Iqbal | Dr. Muhammad Nasir | Dr. Saima Inayat.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Diabetes is metabolic disease in which there are high glucose levels over a long period of time. The principle symptoms of high glucose are expanded thirst, regular pee, and expanded craving. Diabetes is a disease that influences the way the body uses food from the blood. It is normally a hereditarily issue of digestion system, that is showed by the inability to tolerate carbohydrates and insulin inadequacy. In 2014 around 387 million individuals have diabetes the whole way across the world and 90% of the cases were of Type 2 diabetes which is equivalent to 8.3% of the grown-up population. This proportion was equivalent for both ladies and men. From years 2012 to 2014, it is evaluated that 1.5 to 4.9 million deaths each year was because of DM. The danger of death is twice with diabetes. The normal number of individuals with diabetes is 592 million by 2035 by this rate. With more than 16 million individuals America have some type of this disease (DM). 700,000 new instances of DM are analyzed each year. Diabetes is increasing at very high rate in Pakistan. There are nearly 7.1 million DM patients in Pakistan and it is also estimated that if DM continually develop at this rate then 11.4 million patients of diabetics will be there and Pakistan would rank 10th in the world in 2030. This study is designed due to this alarming rate of diabetes in our country. Inulin present in chicory roots may be used to optimize blood glucose level in diabetic patients. Total 20 diabetic participants aged 30 to 40 years, present in Lahore, were selected for this study in two groups with 10 each participants. Chicory root powder was used as a source of inulin. 60 Chicory roots was purchased from the market. Then cleaned from dirt and then it was dried with the help of oven at temperature 55˚c.Then this dried roots were grinded with the help of grinder machine. Two group were formed. One group was control group that used only allopathic medicines specifically for diabetes. Other group was inulin group that used chicory root powder. Inulin group used both chicory root powder and medicines 1st week of the study. This was done only to ensure no side effect of chicory root powder on the participants. Next week when there was no adverse effect then participants were advised to use half of the medicine along with the chicory root powder. Then after 13 days when glucose level of the participant was on the course then eliminated the medicines and told them to use only 10 grams of chicory root powder daily. Glucose level of these patients was tested daily by the glucometer for two weeks. Then after every 5 to 7 days for 3 months. Then at the end of this period HBA1C test was carried at the hospital laboratory. In this experimental trial, we investigated the effect chicory root supplementation especially inulin has on glucose level of type 2 diabetic patients. Our results showed that three months of chicory root supplementation significantly decreased blood glucose level. My study reveals that inulin as a fiber didn’t control the diabetes but have a little effect on the glucose intake in the blood due to its fibrous nature. Data showed that the inulin supplementation slightly reduce the glucose level in the blood. First week of the supplementation was along with medicines and data showed reduction in the glucose level of blood. At initial day the average random blood glucose level was 251.2 ± 60.1 mg/dl and after 7 days it was 220 ± 53.1 mg/dl and at the end of 90th day it was 195 ± 52.3 which shows that inulin has a reducing effect on the diabetes to some 61 extent. In this study we can conclude that inulin containing chicory roots has a positive effect on the reduction of glucose level of blood (p < 0.001). The patients that use 10 grams of chicory root powder constantly were able to lower their blood glucose level and HbA1c. But it cannot be used as a medicine replacement in diabetic patients because it does not control blood glucose level to the normal range. Future Recommendations: I recommend in future that effect of inulin on the diabetic patients should be checked in relation to amount of medicines given to the patients. Researchers should check that what happens when inulin is used with different amount of medicines in diabetic patients. And also what happens when different amount of inulin is given to diabetic patients along with same medicine. Availability: Items available for loan: UVAS Library [Call number: 2568-T] (1).

10. Effect Of Encapsulated Yoghurt Starter Culture On Physico Chemical And Organoleptic Properties Of Yoghurt

by Aamir Shahzad (2008-VA-430) | Dr. Sanaullah Iqbal | Dr. Naureen Naeem | Dr. Saima Inayat.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Yoghurt is a fermented milk product, which is produced by the bacterial fermentation of milk. It is a rich source of calcium, protein and vitamin B-complex. Lactose-intolerant people can eat yoghurt without any harm as lactose is converted into lactic acid by the bacterial culture. Yoghurt is more nutritive than milk and possesses better digestibility. The benefit of yoghurt depends upon the presence of beneficial viable bacterial culture in adequate number. The bacterial cultures are used in the fermentation process to metabolize the lactose, secondly the proteolysis of protein for improving bioavailability and thirdly lactic acid bacteria for production of some B-complex vitamins and vitamin K. Yoghurt cultures are responsible for the production of aromatic flavor compounds. Purpose of this study was to prepare yoghurt using encapsulated starter culture and effect on physico-chemical and organoleptic properties of yoghurt. For this, harvested starter culture was encapsulated using sodium alginate solution. As per this study, yoghurt starter culture remains active at 2% concentration of sodium alginate used for encapsulation process. Physico chemical and sensory attributes remains same as compared to yogurt made by non-encapsulated yoghurt starter culture. Encapsulation technique protects cultures from any condition acidic pH of yoghurt or any other bacteriosins produced by microorganisms. As a result, CFU/ml increased in protected. During storage the prepared yoghurt was evaluated for its sensory qualities. A panel of judges evaluated the yoghurt samples for appearance, taste, color and overall acceptability on 14-point hedonic scale. The sensory evaluation of the product at 0, 7, 14 and 21 day. There was significant effect of storage on sensory attributes of yoghurt made with encapsulated and non-encapsulated yoghurt starter culture. Yoghurts were evaluated for physico-chemical ………………………………………………………………………………………………..Summary 35 parameters (Fat, protein, total solid, acidity and pH ) during storage of 21 days with 7 days interval acidity contents was continuously increased and pH decreased while protein, fat and total solid values remains same all treatments. Feature Recommendation:  Encapsulation can be used for different purposes like probiotic consumption.  By the use of immobilization technique we can repeatedly use encapsulated culture for many times in manufacturing of stirred yogurt. Availability: Items available for loan: UVAS Library [Call number: 2643-T] (1).

11. Extraction Of Functional Polysaccharides From Chickpea (Cicer Arietinum) And Application As Edible Coating On Cheddar Cheese

by Muhammad Ali Sabir (2008-VA-433) | Dr. Muhammad Nadeem | Dr. Saima Inayat | Prof. Dr. Muhammad Abdullah.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Cheddar cheese was collected from the market and coated with three different coatings. All the reagents used in this study were High Performance Liquid Chromatography grade and obtained from Sigma Aldrich, USA. Experiment were performed in completely randomized design. Cheddar cheese was ripened for 60-days for lipolysis and was analyzed at the interval of 30-days interval. Composition of cheese milk and cheese, peroxide value, Iodine value, free fatty acids and changes in fatty acids (short chain and unsaturated fatty acids) composition were determined. Fat content of all the treatments and control went on decreasing throughout the storage period of 60-days. There was not-significant difference in the protein content of cheddar cheese coated with different coatings.Moisture contents of cheddar cheese coated with different coatings decreased with the decreased ripening period of the cheddar cheese (P>0.05). Free fatty acids increased in all the treatments during the entire ripening period from 0 day to 60 days, the concentration of free fatty acids in all the treatments at all the determination time points were not different from each other and control. There was also increase the peroxide value of control sample from 0 day to 60-day time period of cheese ripening. All the determination frequencies showed that peroxide value of control and experimental samples were not different.Iodine value decreased in control sample day 0 to day 60 continuously, the decline in iodine value of control and experimental samples was not different.Concentration of short-chain, medium-chain and long-chain fatty acid decreased during the ripening period of 60 days but the decline in all the treatments was non-significant.Anisidine value increased in control sample day 0 to day 60 continuously. There also increased the Anisidine value higher in all treatments and control (P>0.05). These results suggest that galactomannas based coating can be used for the coating of cheddar cheese. Availability: Items available for loan: UVAS Library [Call number: 2701-T] (1).

12. Determination Of Theobromine In Chocolates And Chocolate Cakes

by Zain Amin (2010-VA-383) | (Dr. Zubair Farooq) | (Dr. Sanaullah Iqbal) | (Dr. Saima Inayat).

Material type: book Book; Literary form: not fiction Publisher: 2017Dissertation note: Chocolate is very famous food in the whole world especially among the children. Theobromine is one of the main constituent of chocolate and its powders. It is a stimulant and its excessive use causes toxicity or poisoning to the human beings. In Pakistan consumption of chocolate especially by children is very high so chances of chocolate poisoning are also very high. Therefore this study was planned to determine the theobromine levels in locally available chocolates and chocolate cakes in Pakistan. The research was carried out in the laboratories of the Department of FSHN, UVAS, Lahore under the controlled conditions. Sixty samples (40 chocolates, 20 chocolate cakes) of chocolates and chocolate cakes were collected through random sampling technique from locally available super stores and bakeries of Lahore City. The determinations of theobromine in chocolate were carried out through UV spectrophotometric method. Absorption peaks were checked at the 272.7nm wavelength and calculated using Beer’s law. Total sample were 180 and they were analyzed in triplicates. Data were analyzed through the Microsoft Excel 2010 and SPSS 22.0. Mean values with standard deviation in percentages of results were analyzed by descriptive analyses. To examine the relationship among the variables of chocolates and chocolate cakes chi-square test was used. Further to compare theobromine levels in the local and branded chocolates obtained from the common and renowned bakeries of Lahore, independent “t” test was used. Summary 49 The study concluded that local chocolates had more theobromine as compared to the branded chocolates and common bakeries chocolate cakes less theobromine level than the renowned bakeries chocolate cakes. In the current study, mean values of theobromine ranged from 0.55 to 2.32 mg/g for Haribo and Sonnet chocolate samples respectively. The percent safe level of various chocolate samples showed that 45 % chocolates were at the safe level while 55 % unsafe. Similarly, mean values of theobromine ranged from 0.58 to 2.27 mg/g for Sheraz Bakers chocolate cake and Fresh Inn Baker chocolate brownie samples respectively. The percent safe level of various chocolate cake samples showed that 80 % chocolate cakes were at the safe level while 20 % unsafe. Availability: Items available for loan: UVAS Library [Call number: 2861-T] (1).



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