1.
Effect Of Different Feeding Regimens On Subsequent Growth, Performance, Carcass Characterstics And Immune Response In Sexed Broiler
by Muhammad Akif Akram (2009-VA-182) | Dr. Shahid Mehmood | Prof. Dr. Athar Mahmud | Dr. Shafqat Nawaz Qaisrani.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Protein is a major component of broilers diets. There are contradictory reports in literature regarding optimum levels of protein in broiler chicken diets to obtain reasonable performance. Initially some efforts were made to establish a minimum level of protein to get optimum performance but those moves resulted in poor performance in terms of weight gain, feed intake and feed conversion ratio. The present study was conducted at Poultry Research and Training Centre (PRTC), Ravi Campus Pattoki, University of Veterinary and Animal Sciences, Lahore. The duration of the proposed study was 35 days. In total, 360 day old commercial broiler chicks were assigned to 12 groups in 3×4 factorial arrangement under Completely Randomized Design (CRD). Treatments consisted of 3 sex rearing systems (male, female and mix) and 4 feeding regimes (HD, conventional, NRC and Soya Corn). Each treatment was replicated 3 times with 10 birds in each. Data regarding growth performance incuding feed intake, body weight and FCR were recorded on weekly basis. At the end of trial, from each replicate two birds were randomly picked up and slaughtered to collect the data for carcass characteristics. After slaughtering, 5 ml blood was collected in test tubes from Jugular vein of each bird. Serum samples were extracted and stored at -20C for measuring antibody titers against and serum chemistry. The collected data were analyzed through ANOVA technique under factorial arrangement using PROG GLM. Means were compared through Duncan’s Multiple Range (DMR) test with the help of SAS 9.1.
Broilers reared on different feeding regimes showed significantly enhanced body weight, better FCR, PEF, PS, PI and better ND titer in high density (HD) diet fed birds, whereas feed intake was found to be higher in those birds which were fed at conventional diet. On the other hand, glucose and cholesterol showed non-significant results. While carcass characteristics showed CHAPTER 6 SUMMARY
SUMMARY
46
higher abdominal fat, heart weight and thymus weight in soya corn based diet whereas pancreas, breast and thigh meat yield weights were found to be higher in HD diet. Different sexes indicated higher body weight, FCR, PEF,PS, PI, dressed weight, heart weight, keel length and shank length in males whereas abdominal fat was observed to be higher in females. Males showed better economics rather than straight run and female birds. Among different feeding regimes HD feed exhibited better economical appraisal as compared to Soya corn, NRC and conventional diet.
Conclusion:
Based on the findings of the present study it may be concluded that
1. Rearing of broilers on high density (HD) feed resulted into enhanced body weight and better FCR. Whereas feed intake was found to be higher in birds fed conventional diet. In sexes, male broilers fed high density (HD) feed showed better body weight, improved FCR, PEF, PS and PI values.
2. In carcass characteristics, birds fed high density diet showed enhanced weight of pancreas, breast meat yield, keel length and thigh yield while abdominal fat, heart weight and thymus weight were higher in soya corn diet fed birds. In different sexes, rearing of males separately indicated increased dressed weight, heart weight, keel length and shank length whereas abdominal fat was observed to be higher in females.
3. Birds reared on HD diet exhibited elevated antibody titer as compared to NRC, Conventional and soya corn based diet however with respect to cholesterol level, different sexes and feeding regimes separately could not show their response.
SUMMARY
47
4. Among different feeding regimes, high density (HD) diet remained more economical than Soya corn, NRC and conventional diet. In sexes, rearing of males separately remained more economical than rearing of females alone as well as combined sex rearing.
Recommendations for the farmers
Based on the current findings, here are some recommendations for the farmers
1. HD feed can improve the growth performance and carcass characteristics.
2. HD feed is cost effective so by using the high density feed better results can be obtained.
Suggestions for the futures researchers
Same treatments can be applied in sexed broilers to evaluate meat quality and organoleptic.
Future researchers can also apply different feed regimes in different broilers strains. Availability: Items available for loan: UVAS Library [Call number: 2509-T] (1).
2.
Impact Of Digestible Lysine, Methionine + Cysteine Ratios On Growth Performance, Ileal Digestibility Of Protein And Gut Health In Broilers Fed Different Protein Sources
by Ch. Ahsan Akram (2009-VA-397) | Prof. Dr. Talat Naseer Pasha | Dr. Shafqat Nawaz Qaisrani | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Poultry production is a vibrant segment of agriculture in Pakistan. Genetic selection and nutritional improvements resulted in accelerated growth rate and better FCR in modern broilers. This improved broiler performance requires greater amount of digestible nutrients. Since feeding cost accounts about 70% of total production cost and protein is the most expensive component of poultry diets. Future poultry diets, therefore, will be formulated by using cheaper and poorly digestible protein sources such as CM and SFM due to price volatility of highly digestible but expensive sources like SBM. The undigested protein in hind gut is subjected to microbial fermentation, results in formation of nitrogenous products that are not only harmful to birds, but also excreted into litter bedding and generate ammonia, nitrous acid and nitrous oxide, the latter one is a powerful greenhouse gas with an effect about 300 times compared with carbon dioxide. The potential solution to this problem is supplementation of synthetic amino acids in low nutrient density (CP) diets formulated by using cheaper and poorly digestible protein sources. Therefor a study was designed with the objectives to determine the impact of dLys, M + C ratios on growth performance, apparent Ileal digestibility of protein and gut health in broilers fed poorly digestible protein sources (CM and SFM). An experiment with 432 mixed sex (Ross 308) 1-d-old broilers was conducted to test the hypothesis that supplementation of Meth may improves growth performance, apparent Ileal digestibility of protein and gut health in broilers fed poorly digestible protein sources (CM and SFM). The interaction effects of the protein source (CM vs. SFM), CP levels (high vs. low) and dM+C/d Lys (recommended vs. supplemented) were tested in a factorial arrangement of 8 (2 × 2 × 2) dietary treatments. The supplementation of Meth positively influenced FI (P = 0.042), BWG (P = 0.003) and FCR (P <0.001). The broilers fed Meth supplemented diets had, on an average, 10% greater apparent Ileal digestibility of protein, 34% longer villi, 24.5% shorter crypts and 60% greater VCR compared with those fed diets with recommended levels of Meth. The levels of CP, similarly, influenced (P <0.001) the FI, BWG and FCR. The broilers fed high CP diets had 9% improved apparent Ileal digestibility of protein compared with those fed low CP diets. In conclusion, supplementation of Meth improves the broiler growth performance, apparent Ileal digestibility of protein and gut health fed poorly digestible protein sources (CM and SFM). The negative effects of poorly digestible protein sources thus may be partially ameliorated by supplementation of Meth.
CONCLUSIONS
In conclusion, the negative effects of poorly digestible protein sources thus may be ameliorated by supplementation of Meth. Supplementation of Meth above the recommended levels improves the growth performance, Ileal digestibility of protein and gut health in broilers fed poorly digestible protein sources (CM and SFM).
SUGGESTION AND RECOMMENDATION
Further research is needed to evaluate the different strategies to improve the digestibility of poorly digestible protein sources including guar meal. Threonine also has an important role in gut health so there is a need to study the effects of supplementation of threonine above the recommended levels in broilers fed poorly digestible protein sources. Feed form has pronounced effects on gut development, digestibility of nutrients and subsequent growth performance of birds. There is a dire need to evaluate the other cheaper protein sources, supplemented with synthetic amino acids and to find out a possible interaction between feed form and poorly digestible protein sources that may replace SBM in broilers.
Availability: Items available for loan: UVAS Library [Call number: 2567-T] (1).
3.
Effect Of Post Pelleting Liquid Phytase Supplementation On Growth Performance And Phosphorous Utilization In Broilers
by Muhammad Balal Munawar (2014-VA-516) | Prof. Dr. Saima | Dr. Shafqat Nawaz Qaisrani | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Poultry sector is one of the most developed and vibrant segment of agriculture sector in
Pakistan and is claimed to be among the largest industries of Pakistan with over more than 200
billion rupees investment and offering employment directly or indirectly to 1.5 million people. In
vegetable sources used in poultry rations, P is mostly present in phytate form. Phytate P is not
digested by poultry due to lack of endogenous phytase enzyme. So exogenous phytase are now
added in feed to release P from phytate. Phytase is mainly derived from fungi (Aspergilus niger)
and bacteria (Escherichi coli). Phytase starts work either at 3rd C (3-Phytase) or at 6thC (6-Phytase)
of phytate Phytase mostly used in pre pelleting powder form but it has some activity issues during
high pelleting temperature. Steam application is the most important factor that reduces enzyme
activity due high steam temperature and addition of moisture ultimately lead to enzyme inactivity.
Post pelleting liquid enzyme can be one solution to combat enzyme activity issue.
In total, 504 1-d-old chicks were procured from a commercial hatchery and randomly
assigned to six dietary treatments with Hubbard straight run n=84 chicks per treatment. Each
treatment was further divided into 3 replicates with 28 birds/replicate. One treatment was served
as positive control, while second was served as negative control with (0.30 and 0.26 %) P in starter
and grower respectively. Other four diets had two levels (500 and 750 FTU/kg) of each powder
and liquid enzyme. Liquid enzyme supplementation was done post pelleting. Pelleting temperature
was kept at 85o C.
Feed intake, body weight gain and FCR was recorded at the end of each week. At 35th day of
experiment, 3 birds per replicate were selected randomly, weighed and slaughtered. At the time of
slaughtering, blood samples were collected in heparinized tubes for the determination of plasma
SUMMARY
40
P.Ileal digesta from the slaughtered birds was collected for the determination of P digestibility.
The visceral organs and left tibia from the slaughtered birds were collected, organ weight was
recorded and tibias were ashed for determination of Calcium and P.
Data collected was analyzed using analysis of variance (ANOVA) technique under
Completely Randomized Design (CRD) and means were compared through DMR test by
using SAS 9.1 version (SAS 2001).
The conducted study showed 49.45%, 64.64%, and 8.64 % higher results in feed
intake, body weight gain and FCR when best group H compared with group A (NC). Liver
and heart weight increase in group A, however gizzard weight showed non significant
(p>0.05) results among all groups. Tibia ash, Phosphorous, calcium and plasma P showed
significant results (p<0.05) when all phytase supplemented diets compared with NC. Availability: Items available for loan: UVAS Library [Call number: 2631-T] (1).
4.
Effects Of Digestible Lysine Levels On Production Efficiency, Protein Digestibility, Gut Health And Carcass Characteristics In Broilers Fed Different Protein Sources
by Muhammad Jhazaib Arslan (2014-VA-956) | Dr. Shafqat Nawaz Qaisrani | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: In Pakistan, poultry is a vibrant part of agriculture. Nutritional and genetic improvements resulted in accelerated PEF in broilers. This improved broiler production efficiency requires greater amount of digestible nutrients like digestible amino acids. Feeding cost accounts for 70% of total cost and protein is the most expensive component of broilers ration. Future poultry rations will be formulated by using poorly digestible and cheaper protein sources such as SFM and CM due to price instability of highly digestible sources like SBM. The undigested protein in hind gut subjected to microbial fermentation, results in formation of nitrogenous products that are harmful to birds. The possible solution to this problem is supplementation of synthetic amino acids in low nutrient density (CP) diets formulated by using cheaper and poorly digestible protein sources. Therefore a study was designed with the objectives to determine the impact of dLys on production efficiency, TTP digestibility, gut health and carcass characteristics in broilers fed poorly digestible protein sources (CM and SFM). An experiment with 624 Ross 308 1-d-old broilers was conducted to test the hypothesis that supplementation of dLys may improve production efficiency, TTP digestibility, gut health and carcass characteristics of the broilers fed poorly digestible protein sources (CM and SFM). The interaction effects of the protein source (CM vs. SFM), CP levels (high vs. low) and dLys (high, medium and low) were tested in a factorial arrangement of 12 (2 × 2 × 3) dietary treatments and 1 control treatment. The supplementation of dLys positively influenced production efficiency (P <0.001). The birds fed diets containing 19% CP showed 8.53% greater TTP digestibility compared with those fed diets with 17% CP, however, VH was influenced by protein source (P<0.001) and CP level (P = 0.030), however, not influenced by dLys levels while CD was influenced by dLys levels (P = 0.023). The birds fed 1.16% dLys supplemented diet had a 9.6 and 7.5% greater dressed weight with giblets compared with those birds fed diets with 1.10 and 1.21% level of dLys. In conclusion, supplementation of dLys improves the broiler production efficiency fed poorly digestible protein sources (CM and SFM). The negative effects of poorly digestible protein sources thus may be partially ameliorated by supplementation of dLys.
CONCLUSIONS
Supplementation of dLys above the recommended levels (1.16%) improves the production efficiency, TTP digestibility of protein, gut health and carcass characteristics in broilers fed poorly digestible protein sources (CM and SFM). In conclusion, the efficiency of poorly digestible protein sources may be improved by supplementation of dLys at level of 1.16%.
Availability: Items available for loan: UVAS Library [Call number: 2765-T] (1).
5.
Influence Of Digestible Lysine Levels On Growth Performance, Gut Development And Ileal Digestibility Of Two Vegetable Protein Sources Fed To Broiler Birds
by Anjum Faiz (2014-VA-955) | Dr. Shafqat Nawaz Qaisrani | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: The poultry sector is playing a substantial role in agriculture sector of Pakistan. The extensive
research in poultry has brought vital changes that accelerated growth and improved FCR. Poultry
diets are majorly formulated cost effective for the sustainable poultry industry. For this reason,
the main focus is on protein sources which, if maintained to an adequate level with the
requirement results in cost effective feeding. In the coming days, main focus of the nutritionists
would be on replacement of expensive sources of protein during feed formulation. For this
reason, their first choice may be the selection of poorly digestible protein sources such as CM
and SFM. These sources are cheaper as compare to the SBM which is imported hence increasing
the feed cost. The poorly digestible protein sources, furthermore, in hind gut may be subjected to
microbial fermentation, results in formation of nitrogenous products that are not only harmful to
birds, but also excreted into litter bedding and generate ammonia, nitrous acid and nitrous oxide,
the latter one is a powerful greenhouse gas with an effect about 300 times compared with carbon
dioxide. The potential solution to this problem is supplementation of synthetic amino acids in
low nutrient density (CP) diets formulated by using cheaper and poorly digestible protein
sources. For this reason, a study was designed with the objectives to determine the impact of
dLys, on growth performance, ileal digestibility of protein and gut development in broilers fed
poorly digestible protein sources (CM and SFM). An experiment with 624 mixed sex 1-d-old
broilers (Ross 308) was conducted to test the hypothesis that supplementation of dLys may
improves growth performance of the broilers fed poorly digestible protein sources (CM and
SFM). The interaction effects of the protein source (CM vs. SFM), CP levels (high vs. low) and
dLys (recommended vs. supplemented) were tested in a factorial arrangement of 12 (2 × 2 × 3)
dietary treatments and SBM as a control diet was used. The results indicate the birds fed diets
DISCUSSION
42
containing CM and SFM supplemented with dLys has significant effect on growth performance
in broilers. The results of the study showed that the FI, BWG and FCR for CM and SFM based
diets was different during starter and grower phases. The FI and BWG FCR was influenced by
CM and SFM based diets. The FI was not influenced by dLys levels during starter phase. The
BWG and FCR was improved with supplementation of dLys levels. During starter phase FI and
BWG was not influenced by CP levels, however, FCR was improved. The results of the grower
phase indicate that FI was significantly influenced by protein sources, CP levels, as well as, by
dLys levels. The BWG, similarly, was significantly influenced by protein sources, CP levels, and
dLys levels. The FCR during the grower phase was improved by increasing CP level. The FCR
was also improved when fed CM based diets. Supplementation of dLys significantly influenced
FI and BGW during grower phase. The FCR was also improved with the supplementation of
dLys levels. The results indicate that the birds fed dLys supplemented diets had significant effect
on ileal digestibility of protein. The results of the study showed that ileal digestibility for protein
was not influenced by CP levels. The present study resulted that some of the organs are
significantly influenced by dLys levels and CP levels, whereas, other are in influenced by protein
sources. Amino acid supplementation showed significant difference on ileal digestibility of
protein and gut development in broilers. The negative effect of poorly digestible lysine can be
compensated with the supplementation of synthetic amino acids including lysine.
CONCLUSIONS
Supplementation of dLys above the recommended levels improves the growth performance, ileal
digestibility of protein and gut development in broilers fed poorly digestible protein sources (CM
and SFM). In conclusion, the negative effects of poorly digestible protein sources in broilers may
be ameliorated by supplementation of dLys. Availability: Items available for loan: UVAS Library [Call number: 2776-T] (1).
6.
Effect Of Different Levels Of L-Valine On Production Performance, Serum Biochemistry And Illeal Digestibility Of Protein In Laying Hens (Lsl - Lite)
by Usman Liaqat (2015-VA-1092) | Dr. Yasir Allah Ditta | Dr. Shafqat Nawaz Qaisrani | Dr. ShahzadAli.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Valine is a limiting branched amino acid in corn soya layer diet.It has both properties of glycogenic and ketogenic. It is also important in protein synthesis and precursor for many amino acids. A very little research has been conducted on levels of valine in layer strains globally, particularly research data is limited in Pakistan. So far, no trial has been conducted to find out the effect of different levels of valine for LSL lite in Pakistan.
A total of 490 laying birds of LSL (LITE) strain of 33 weeks of age were selected and categorized into seven dietary treatments with different levels of Valine i.e. 0.70, 0.75, 0.78, 0.81, 0.84, 0.87 and 0.90 % of feed. All experimental diets were fed in mash form for 56 days of experimental period. Celite was added at rate of 2 % of feed for crude protein digestibility determination. At the end of experiment, two birds per replicate were randomly selected and slaughtered for the collection of blood and illeal digesta. Egg weight and egg production were calculated on daily basis. Three egg were selected from each replicate to measure egg quality parameters at end of each week. The egg quality parameters were calculated on weekly basis and body weight was measured at beginning, mid and end of the experimental period.At day 56, two birds from each replicate under different treatments were randomly selected and slaughtered to collect sample for ileal protein digestibility and blood for serum biochemistry. Ileal samples were
stored at -20C. Serum samples were extracted and stored at -20C for measuring serum chemistry. Collected data were subjected to ONE WAY ANOVA under Complete Randomized Design. Duncan’s multiple range tests (DMR) were used to compare means. Statements of statistical significance are based on P < 0.05. Weekly collected data of egg quality parameters were analyzed by repeated measures SAS (version 9.1; SAS Inst. INc., Cary, NC).
The current study shows significant influence of valine on production performance of laying hens. With increase in valine levels in the diet of laying hens, the egg production, FCR and feed intake significantly improved.Valine has significantly enhanced egg quality parameters i.e. egg weight, Haugh unit, egg yolk color, specific gravity, proportional yolk weight, proportional shell weight, eggyolk index and egg yolk to albumen ratio. Significant effects on total serum protein, serum albumin, immunity and ileal digestibility of proteins have been observedin laying hens. Following results shows that valine is limiting amino acid afterthreonine in diets devoid of animal protein in corn soya bean dietand must be given in diet for maximum production.
Conclusion
It can be concluded that valine is a limiting branched chain amino acid in corn soya layer diet. Supplementation of valine significantly increase egg production, improved FCR / dozen of laying hens. An increase in valine levels also significantly decreases the feed intake in laying hens which may be due to the antagonistic effects with BCCAA levels. Valine improved egg quality parameters (proportional yolk weight, proportional shell weight, egg weight, yolk color, specific gravity, yolk index and yolk to albumen ratio, Haugh unit) of laying hens. Influence of Valine has been observedon serum biochemistry (serum albumin, serum total protein and ND titer) of laying hens.
Recommendations for the farmers
Based on the current findings, 0.87 % valine is recommended level for maximum egg production at the age of 33-40 weeks of age with dig Val : dig Lys ratio of 1.04 : 1.
Suggestions for the future researchers:
1. Same experiment can be done in broilers to assess growth performance, serum biochemistry and ileal digestibility of protein.
2. Future researchers can also apply this research in different layer strains.
Availability: Items available for loan: UVAS Library [Call number: 2857-T] (1).
