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1. Role Of Single Injection Of Prostaglandin F2 Alpha On Breeding Efficiency Of Buffaloes

by Sajid Iqbal | Prof. Dr. Muhammad Aleem Bhatti | Dr.Imtiaz Hussain Khan | Dr.Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Nature of contents: biography; Literary form: Publisher: 1998Dissertation note: In the present study, a total of twenty Nili-Ravi buffaloes were divided into two equal groups. In group A ten buffaloes were administered with prostagladin F2 alpha (Lutalyse, Upjohn), 2 hours after calving. In group B, ten buffaloes were not given any treatment and designated as control. The reproductive organs of each experimental buffalo were rectally palpated on day 14 and day 21 postpartum. After that twice a week rectal palpation was carried out until the first postpartum oestrus. The results of present study revealed that cervical and uterine involution was completed significantly (P < 0.05) earlier in group A as compared to group B (28.90± 1.79 and 35.40±3.95 days). There was no significant difference in the diameter of cervix, gravid and nongravid uterine horn at day 14 postpartum. A significant difference between the groups was obtained on days 21, 25 and 28 postpartum in the diameter of cervix and gravid horn. The corpus luteum (CL) of pregnancy regressed very rapidly following calving. The overall period required for complete regression of corpus luteum of pregnancy was (19.20±4.87 days) in treated group and (18.40±6.07 days) in control groups. The difference was significant. Follicular activity resumed independently of uterine involution. It was, however, delayed slightly by the retained corpus luteum of pregnancy. The mean postpartum interval of initial follicular development was 21.20±5.71 days in treated and 28.20±8.75 days in control groups, respectively. The difference was statistically significant (P <0.05). Postpartum oestrus interval was shortened in treated group (79.50±19.83 days) as compared to control group (103.0± 17.45 days) and the difference was significant (P<0.05). So it seems beneficial to administer prostaglandin F2 alpha in postpartum buffaloes to reduce the period for uterine involution and enhance the subsequent reproductive performance. Availability: Items available for loan: UVAS Library [Call number: 0585,T] (1).

2. A Study On The Effect Of Gnrh Analogue On Anovulation In Repeat Breeding Cross Bred Cows And Their Conception Rate

by Asif Rafiq | Prof.Dr.Rashid Ahmed Ch | Dr.Imtiaz Hussain Khan | Dr.Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1999Dissertation note: The incidence of repeat breeding is high in cross-bred cows due to low LH level in the blood at the time of estrous. In this study Dalmarelin a GnRH analogue which induces ovulation in cows was used. Nine healthy animals with the history of repeat breeding and clinically normal genital tract were selected at the Livestock Production and Research Institute, Qadirabad, Okara. These animals were injected with dalmarelin (Lecirelin), at the dose rate of 2 ml IM. The injection was given on the zero day of estrous and later animals were inseminated artificially. Two blood samples were collected from these animals, one before the injection and artificial insemination i.e. on the zero day of estrous and the second at 10th day after the injection of Lacirelin for the detection of plasma progesterone concentration. Results indicated that five animals i.e. 55.5% had conceived. Similarly when detected 12 hours after the end of estrous five animals had ovulated out of which three animals were conceived. Finally, these animals were palpated rectally on the 90th day after artificial insemination and five animals with high concentration of progesterone on the 10th day, were, detected positive for pregnancy. None of the control animals ovulated and conceived. Availability: Items available for loan: UVAS Library [Call number: 0623,T] (1).

3. Comparative Reproductive Performance Of F1 & F5 Filial Groups Of Holstein Friesian X Sahiwal Crossbred Cows

by Tariq, M | Dr.Rashid Ahmad Chaudhry | Dr.Imtiaz Hussain Khan | Dr.M.Sarwar | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 2000Dissertation note: Data of one hundred animals of two filial groups ½ (Fl and F5) had been analysed in this study. The animals were maintained at Livestock Production Research Institute, Bahadarnagar, Okara during the year 1981-1999. The data was analysed to estimate the magnitude of different reproductive traits, namely, the age at maturity/puberty, age at first conception, age at first calving, service period, calving interval, gestation period, dry period, number of services per conception and conception rate. The average mean values for the filial groups 34 (Fl and ES) of Holstein Friesian x Sahiwal crossbred cows i.e., Age at maturity, Age at first conception and Age at first calving were 725.98 days or 84 months and 740.95 days or 24 months, 761.54±216.67 days or 25 months and 789.73±138.64 days or 26 months and 1040,7±218.47 days or 34 months and 1069.60± 139.92 days or 36 months respectively. The average mean values for Service period and No. of service per conception were 178.90±114.04 days and 222.93±163.67 days and 2.15± 1.10 and 2.20± 1.15 respectively. For gestation period and Calving interval the average mean values were 279.19±8.0 days or 9 months and 280.53±7.8 days or 9 months and 459.54±115.54 days, 522.04±162.46 days respectively for the filial groups ½ (Fl and F5) of the Holstein Friesian x Sahiwal crossbred cows. The average mean values for the Dry period and Conception rate in the present studywere 155.79±104.54 days, 215.40±139.26 days and 2.15±1.10% and 2.20±1.15% for the ½ (Fl and F5) respectively. The present study indicates that there is non-significant difference among the following reproductive traits of filial groups ½ (Fl and F5) i.e., Age at maturity/puberty, Age at 1st conception, Age at 1st calving, Gestation period, No. of services per conception and conception rate. This study describes that there is a significant difference among these reproductive traits of ½ (Fl and F5) filial groups of Holstein Friesian x Sahiwal crossbred cows i.e., Calving interval, Service period and Dry period. The main object of the crossbreeding was to evolve a new breed of cattle and even upto fifth generation the results are encouraging. By the encourgining results of this study it is recommended/suggested that at present about 2 million heads of non-descript cattle population having very low genetic potential may be come useful by inseminating with exotic semen of Holstein Friesian. The crossbred animals have much superior germplasm as compared to non-discript animals. Further, it is recommended to maintain fifty percent exotic blood level, which is best suited in the subtropical environmental countries like Pakistan. Availability: Items available for loan: UVAS Library [Call number: 0633,T] (1).

4. Role Of Prostaglandin F2 Alpha And Gonadotrophin Releasing Hormone In Induction Of Oestrus In Postpartum Anoestrus Nili Ravi Buffaloes During Summer

by Liaqat Ali | Dr.Imtiaz Hussain Khan | Dr.Muhamad | Dr.Muhammad Aleem | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 1998Dissertation note: In this experiment, twenty four Nili-Ravi buffaloes 60 days postpartum having history 60 normal postpartum were used. These buffaloes were randomly divided into two equal groups (n=12). Treated buffaloes received single injection of 5mg etiproston at 60 days postpartum. And at 78 days postpartum a single injection of gonadotrophin releasing hormone (GnRH) 0.05mg intramuscularly was given to only those buffaloes which did not showed oestrus after etiproston injection. The observation on different aspects like oestrus response, heat detection, interval to onset of oestrus, artificial insemination and pregnancy diagnosis were studied. The buffaloes in group-1, 75% (n=9) exhibited oestrus. The PGF2atreated buffaloes 16.66% (n=2) exhibited oestrus and GnRH treated buffaloes 70% (n 7) exhibited oestrus. The buffaloes in group II 16.66% (n2) exhibited oestrus. The behavioural signs noted were; acceptance of teaser bull, frequent urination, bellowing, licking of external genitalia, mounting by other buflaloes, and raised tail. The physical changes recorded were, uterine tone, swollen vulva, vaginal mucous discharge and vaginal hyperaemia. Oestrus symptoms were more pronounced in treated group than control groups. The buffaloes in treated and control groups, the average number of days from parturation to first oestrus were 81±3.61 and 107±20 days respectively, that was significant (P <0.05) difference between two groups. In PGF2a and GnRH treated buffaloes exhibited oestrus during 62.5±0.5 days and 86.28±1.21 days postpartum respectively. In treated group, the interval to onset of oestrus after injection was 6.22±1.16 days. In control group days from beginning of experiment to onset of oestrus were 47±2 days. Duration of oestrus in treated group was 24±2.11 hours. In PGF2cz and GnRH treated animals the duration of oestrus was 25±3 hours and 24.28±2.67 hours respectively. In control group the duration of oestrus was 18±2 hours. The pregnancy rate in treated and control groups were 44.4% and non of control group conceived. Availability: Items available for loan: UVAS Library [Call number: 0669,T] (1).

5. Serological Surey Of Brucellosis In Man & Various Age Groups Of Cattle And Buffaloes

by Abbas Chaudhry, M | Dr.Irshad Ahmad Ch | Dr.Imtiaz Hussain Khan | Prof.Dr Akram | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 2000Dissertation note: The sero-prevalence of brucellosis in cattle, buffaloes and various personnel employed at Livestock Production Research Institute, Bahadar Nagar, District Okara, was surveyed. Four hundred and seventy blood samples of different age groups of various groups of cattle, buffaloes and man were analyzed for the seroprevalence of Brucellosis. The various serological tests used for this investigation included the Rose Bengal Plate Test (RBPT) and Serum Agglutination Test (SAT). The higher incidence of the disease was observed in cattle than buffaloes. The seroprevalence of disease in man was found to be related with the prevalence of Brucellosis in animals. The overall prevalence of Brucellosis in clinically healthy buffaloes and cattle was found to be 7.64% and 10.0% respectively, by SAT and 11.17% and 14.11% respectively by RBPT. A prevalence of 36.67% by RBPT and 26.67% and 23.33% by SAT in clinically sick buffaloes and cattle was recorded. The serum samples from workers subjected to RBPT and SAT revealed prevalence of 15.71% and 11.43%, respectively. The incidence of Brucellosis in adult buffaloes and cattle above 2-10 years of age was 11% and 13%, respectively by SAT. Whereas in young buffaloes and cattle under 2 years of age, it was found to be 2.86% and 5.71%, respectively by SAT. The RBPT indicated 15% and 5.7 1% incidence of the disease in adult and young buffaloes, respectively. Similarly prevalence of 19% and 7.14% in adult and young cattle, respectively was found to be demonstrated by RBPT. rpIe SAT and RBPT established a greater incidence of brucellosis in female animals than male cows 10.67% and buffaloes 8.67% by SAT. Availability: Items available for loan: UVAS Library [Call number: 0673,T] (1).

6. Comparativge Efficacy Of Gnrh And Pgf2A For Oestrous Synchronization In Nili Ravi Buffalo

by Abdohoo Abdali, M | Dr.Imtiaz Hussain Khan | Dr.Masood | Dr.Rashid Ahmad Ch | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 2000Dissertation note: Buffaloes occupy an important role in agricultural economy of Pakistan as a triple purpose (milk, meat and draught) animals. Total population of buffalo in Pakistan is 21.6 million. '1'oal milk production in the Pakistan is 24,874 metric tons, out of which 16,665 in Punjab and the share of buffalo is 72.12%. In buffalo husbandry seasonality in breeding is a problem of great importance, because seasonal variation in breeding, as its effects on calving profoundly affects the economy of milk production also. The appearance of oestrous in buffalo is at the maximum (37.56%) during October to December. Therefore, population of the country parturate from July to October. Eventually, during the summer months there is a marked decline in total milk production but demand of milk escalates. To device ways and methods to overcome summer shortage of milk we designed this project to change the breeding pattern of buffaloes by oestrous cycle synchronization in a way to have maximum milk yield during summer months. In this study 30 non-suckling postpartum Nili-Ravi buffaloes were used having average age 2592.66±127.41 days and average weight 464.7U,±8.27 kgs. The 30 buffaloes were randomly divided into two groups viz group A and B, having 15 animals in each group respectively. The buffaloes in group A (n=15) received intramuscular injection of Dalmazine, analogue of PGF2 a 2CC, without palpation at 11 days interval. In group B (n=15) received intramuscular injection of Dalmarelin, Analogue of GnRH 2CC without palpation at 11 days interval. All the animals were observed for (a) Response of treatment (b) interval to the onset of oestrous post-treatment (c) Numbers of buffaloes showing symptoms of oestrous (d) duration of oestrous (e) conception rate (0 cost comparison of both injections per animal. From the present experiment it was observed that, the response of oestrous in group A was 86.66% and in group B was 80%, the interval of oestrous in group A was 3.53±0.32 days while in group B was 12.5±0.34 days. The duration was 21.69±0.53 hours in group A and 20.9±0.67 hours in group B. The symptoms was frequent urination, bellowing, segregation from the herd, acceptance of teaser bull, vulvular sniffing, vulvular swelling and mucous discharge in both groups. The conception rate was 76.92% and 75% in group A and B respectively. The cost comparison was Rs.420 in group A and Rs.452/animal in group B which was more in group B, I .e. Rs.32/animal. It was concluded that PGF2 is more effective for oestrous synchronization, by less time, high conception rate, high percentage of response. However, a detailed study is required to determine the blood hormonal levels after the induction of both the injections, for better improvement. Availability: Items available for loan: UVAS Library [Call number: 0796,T] (1).

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