1.
Disgnosis Of Theileriosis In Buffalo Through Blood Smear Examination And Pcr In District Lahore
by Mukhtar Ahmad | Dr.Jawaria Ali Khan | Prof.Dr.Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2008Dissertation note: The present study was designed to determine diagnosis and infection percentage of
Theileriosis in buffalo in and around District Lahorë, and to design the primer for Theleria
parasite with Microscopic Examination. For this purpose blood samples were collected from
300 buffaloes randomly from 20 villages, during the month of May, June, July and August of
2007 in and around District Lahore. Screening was done by blood smears, stained by Giemsa’s
staining technique and later the blood sample from same animals was also processed by PCR.
The blood smear showed Theileria, piroplasms, including cocci, rod, and signet-ring, with diameter of 0.5-1.5 micrometer. The blood parameter i-e PCV, Hb concentration and TEC showed presence of microlytic hypochromic anemia in diseased animal. On the basis of microscopic examination overall 44.66% (134/300) prevalence was recorded. On the basis of PCR test prevalence of Theleria parasite 5 9.66% (179/300) was recorded show the presence of carrier animal in buffalo population in district Lahore. The result had shown high efficacy of PCR as compare to microscopic examination.
It is anticipated that present study was proved helpful in diagnosis of Theileria in infected as well as in carrier animals in District Lahore and will be beneficial for further study.
Availability: Items available for loan: UVAS Library [Call number: 0983,T] (1).
2.
Detection And Chemotherapy Of Sub Clinical Mastitis In Dairy Catlle And Buffaloes
by Muhammad Jamil | Dr.Jawaria Ali Khan | Dr.Muhammad Iqbal | Prof.Dr.Muham | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2008Dissertation note: The aim of this study was to detect subclinical mastitis in bovine and to find out the most appropriate antibiotic for its treatment. For this purpose, Milk samples were taken aseptically from 500 apparently healthy animals (n= 250 cattle and n= 250 buffaloes) for screening tests. Two different tests, Surf Mastitis Test (SMT) and Somatic Cell Count (SCC) were used. Milk samples declared positive by both of the above mentioned tests, were subjected to culture sensitivity test. Six different antibiotics were evaluated i.e. Enrofloxacin, Norfioxacin, Amoxicillin, Oxytetracycline, Gentamicin and penicillin G. On the basis of sensitivity test; two topmost drugs were selected and be given to two equal groups of animals. Each group was comprised of equal number of cattle and buffaloes. Each antibiotic was given for 5 days as intramuscular (IM) injection. A positive and a negative control groups were also kept. To detect the in-vivo efficacy of antibiotic, again the milk samples of all the groups were examined by SMT and SCC on day 10 and day 20 of first injection. The results obtained were the following;
Sub clinical mastitis was found 42.8% in cattle and 37.6% in buffaloes. Out of
the total of 201 mastitis positive milk samples cultured, bacterial growth occurred in
98 (48.75%) of milk samples. The various bacterial species isolated from milk
samples of cattle and buffaloes were E. coli Staphylococcus, Streptococcus, Proteus,
Kiebsiella, Pseudomonas, and Pasteurella with overall percentage of 50 %, 17.34 %,
3.06 %, 12.24%, 10.20%, 2.04% and 5.10 % respectively.
The in vitro efficacy of Enrofloxacin was found to be the best one i.e. 77.55 % followed by Norfioxacin (67.34 %), Gentamicin (53.06 %), Oxytetracyclin (30.61 %), Amoxicillin (22.44 %) and Penicillin G (4.08 %).
After chemotherapy, there was significant difference between treatment groups and control groups (P<0.05). The difference in the efficacy of enrofloxacin and norfioxacin was statistically non significant (P>0.05), however mathematically, the recovery rate by enrofloxacin was greater (93.75%) then norfioxacin (87.50%). Recovery rate was more at day 20 for both antibiotics.
Availability: Items available for loan: UVAS Library [Call number: 1008,T] (1).
3.
Helminths In Peafowl (Pavo Cristatus) At Lahore Zoo
by Abdul Basit | Dr.Muhammad Sarwar Khan | Dr.Jawaria Ali Khan | Prof.Dr.Muham | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2009Dissertation note: The study was conducted to determine the infection rate of helminths in peafowl and to evaluate the comparative efficacy of two different broad spectrum anthalmintics.
For this puiose, eighty seven different breeds of peafowl like Blue peafowl, Java green peafowl, White peafowl, Black shoulder peafowl, Emerald peafowl and Pide peafowl of different ages, both male and female were selected which were present at Lahore Zoo. Faecal samples of 87 birds were collected in clean polythene bags, properly labeled and examined for the identification of helminths in the laboratory of Clinical Medicine & Surgery department, University of Veterinary & Animal Sciences Lahore. Faecal samples were examined by direct smear and centrifugal floatation methods for qualitative examination. For quantitative examination McMaster Egg Counting technique was used.
Moreover, chemotherapeutic trials were conducted by making four groups of birds. Albendazole (Methyl [5-(Prophylthio)-IH-Benzimidazole-2-yl] carbonate) (Selmore Pharma) at the dose rate of O.lml per k.g. of body weight in drinking water and Pyrantel Pamoate (1 Methyl-2-(2[2-thienylj-l, 4, 5, 6-tetrahydropyrimidine, 4, 4-methylenebis (3- hydroxy-2-naphthoic acid) (Pfizer) at the dose rate of O.lml per k.g. body weight oral suspension only once in the experimental trial was used and there percentage efficacy was determined. Drugs were administered orally to each bird using crop needle.
After the collection and laboratory examination of pre-medicated eighty seven faccal samples, the positive birds of different breeds of peafowl were divided in to three equal groups that were A, B and C. Forty nine out of 87 birds were positive for single or mixed infection of Heterakis gallinae, Ascaridia gaul, Davenia pro glotina, Capillaria columbae and Acuaria spiralis with there individual percentage was 36.73%, 26.53%, 6.12%, 18.37% and 12.24% respectively. Out of 49 birds 48 positive birds were divided in groups, each group consisted of 16 birds respectively. Group 'A' consisted of (Blue peafowl, Java green peafowl, White peafowl, Black shoulder peafowl and Emerald Peafowl, Pide peafowl). Group 'B' consisted of (Blue peafowl, Black Shoulder peafowl, Emerald peafowl and White Peafowl, Java green, Pide peafowl). In group 'C' birds included were (Blue peafowl, White peafowl, Pide peafowl and Black shoulder Peafowl, Emerald peafowl). While in group 'D' (White peafowl, Java green peafowl, Pide peafowl
& Blue Peafowl) only uninfected and untreated birds were kept.
Drug therapy was only induced to group A i.e. Albendazole 0.lml/kg body weight and group B Pyrantel pamoatc 0.lml /kg body weight, while infected but untreated birds were placed in group C.
The overall prevalence of gastrointestinal helminths in different breeds of peafowl pre-medicationwas found as 56.32 %. While the healthy birds were 43.68%. Faecal egg counts were again carried out on day 03, 07 and 10 post-medication and percentage reduction of EPG calculated. On day three the percentage efficacy of Albendazole was 44.76%, on day seven 73.01%, and on day ten 94.92% respectively. In the same way percentage efficacy of Pyrantel pamoate on day three was 29.27%, on day seven 50.13% and on day ten 78.34%.
The percentage EPG rise up to 3.75%, 6.24% and 6.99% at day 03, 07 and 10 in untreated group C was observed. While no infection was observed in group D through out the experimental study. In the current study no mortality of peafowl was found so no postmortem examination was conducted. There was no any side effect of Abendazole and Pyrantel pamoate was found in both the groups which were A & B respectively.
Availability: Items available for loan: UVAS Library [Call number: 1053,T] (1).
4.
Studies On Epidemiological Risk Factors, Treatment Patterns And Effects Of Vaccination Against Peste Petits
by Mahboob Ali | Dr.Muhammad Avais | Dr.Asim Aslam | Dr.Jawaria Ali Khan.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1549,T] (1).
5.
Development Of A Cost-Effective Serodiagnostic Assay For Peste Des Petits Ruminants (PPR)
by Tahira Hanif (2015-VA-1060) | Dr.Jawaria Ali Khan | Dr.Aamer Bin Zahur | Dr. Muhammad Avais | Prof. Dr. Aftab Ahmad Anjum.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Peste des petits ruminant (PPR) is a highly contagious newly developing disease of Small ruminants (sheep and goats). Currently the poor small ruminant’s farmers in Pakistan are facing huge economic losses due to PPR virus. In Pakistan PPR causes economic losses of Rs. 20.5 billion annually. The objectives of present study were to develop a cost effective sero-diagnostic assay for PPR (active haemagglutination inhibition and passive haemagglutination inhibition) and determination of comparative efficacy of active and passive haemagglutination inhibition assay (HI and PHA respectively) for detection of PPR virus infection.
In the present study, n= 300 sera samples were collected from sheep and goats during the (15 Februry 2016 to 2 January 2017).The serum samples were collected from kotli AJK :20(8 goats and 12 sheep),from gilgit:30 serum (20 goats and 10 sheep),from mansehra:22 serum (13 goats and 9 sheep),from mithi:112 (60 goats and 52 sheep) and 116 serum samples (88goats and 28 sheep) from Dhera ghazi khan.None of the animal was known to have been vaccinated against PPR previously or at the time of sampling.
These samples were collected from animals showed symptoms of PPR suggestive of PPR disease as well as from healthy animals. The sera were transferred into sterile tubes and were preserved on ice packs while shifting to the laboratory. PPR virus isolate was originally isolated from an outbreak in Taxila village, district Rawalpindi, the isolate was attenuated serially onto the Vero cell lines up to 20 passages. After, which antigen was titrated using a micotiter haemagglutination (HA) test with chicken RBCs and stored at -70◦c until use as a PPR antigen in a HI test.
In this study Active haemagglutination inhibition (HI) and passive haemagglutination inhibition were developed. The Haemagglutination Assay was standardized by different factors i.e. diluents, Temperature of incubation, Time of Incubation and concentration of Chicken R.B.C̓s. An additional test passive haemagglutination inhibition was performed to check the comparative efficacy of Active and Passive haemagglutination inhibition. In passive haemagglutination inhibition tanned sensitized cells remains effective due to their long effective life when stored at 4̊c and its makes an ideal test for diagnosis of PPR. Newly developed assays were compared against cELISA for PPR using kappa statistics and diagnostic sensitivity and specificity were determined.
The results of both assays were compared with results of competitive enzyme linked immunosorbent assay. In this study cELISA was considered as golden standard. The relative sensitivity and specificity of Active haemagglutination inhibition is 94.9% and 97.9% respectively. (Kappa 0.9264). However the sensitivity and specificity of Passive haemagglutination inhibition is 91.1% and 95.0% respectively.(kappa 0.8595). This study describes the serological detection of PPR virus by Active haemagglutination and passive haemagglutination inhibition (HI and PHA respectively). It was also concluded the comparative efficacy of (PHA and HI) that Active haemagglutination inhibition is more reliable technique than passive haemagglutination inhibition assay for the diagnosis of PPR disease in small ruminants (sheep and goats). Availability: Items available for loan: UVAS Library [Call number: 2816-T] (1).
