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1. A Study On The Effect Of Experimentally Induced Caecal Coccidiosis On Weight Gain Haematological Parameters In Broilers

by Irfan Ullah Khan | Dr.Haji Ahmad Hashmi | Dr.Muhammad Afzal | Dr.Shakil | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1999Dissertation note: The study was designed to observe the effect of experimentally induced coccidiosis on weight gain, FCR and somc blood parameters in broilers. For this purpose 90 day old broiler chicks were obtained from a local hatchery and reared under controlled managemental, coccidia free conditions upto 42 days of age. A commercial coccidiostat free feed and water were provided ad Jib. The birds were divided into three groups A, 13 and C, comprising of 30 birds each. The birds in group A were kept as non-infected, un-medicated control, the group B as infected and unniedicated, while group C as infected and treated with salinomycin (Coxistac 6%; Pfizer). Coxistac 6% at a doze rate of 50 grams per feed bag was provided and the resufts were studied. The chicks of group 13 and C were given oral dozes of 40,000 sporulated oocysts of jjmeria ej.eJi at the age of day 20 and 35. There was no oocyst excretion in faeces of birds in group A, OPG of faeces in group B was higher than in group C. The OPG of faeces reached the peak during 2nd week post-infection in both the infected groups. 'Phe higher mortalit.y in the infected group 13 (20%) than in group-C (1 0%) was observed, while no mortality was recorded in group A. On postmortem it was observed that the caeca were swollen, on incision petcchial hacmorrhagcs were seen on walls of the caeca and lungs and liver of the infected carcasses were dehydrated and pale. The group A showed highest weight gain than groups B and C. however, the group C (infected treated) showed better weight gains than group 13 (Infected). Feed conversion ratio in groups A, 13 and C were significantly different. Group A being uninfected gave the best FCR followed by birds in group C, which were medicated while birds in group B gave the poorest values. The haemoglobin and TEC values in the infected group B were low and were normal for group A. However, the birds of group C also showed lower values as compared to group A. It was observed that the percentage of heterophils and monocytes increased, while the number of lymphocytes, basophils and eosinophils decreased in the blood of the infected groups. Availability: Items available for loan: UVAS Library [Call number: 0603,T] (1).

2. The Prevalence And Taxonomy Of Toxocara Canis Alongwith Associated Haemotological Changes In Naturally

by Chatha, Capt. Muhammad Athar | Dr.Asif Rabbani | Dr.Muhammad Afzal | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2000Dissertation note: Toxocariasis is a common disease of carnivores throughout the world and is caused by Toxocara canis.The larvae of the parasite have been identified as the most frequent etiologic agent of Visceral larva migrans, which is primarily a paediatric disease. In view of the zoonotic importance of the parasite, the present study was conducted to assess the incidence, taxonomy of the species of the genus Toxocara and their effects on various blood parameters of the infected canines. For above purpose, samples were collected from dog hospital, College of Veterinary Sciences, the faecal and blood samples were stored in the refrigerator, of Parasitology Laboratory (C. V. S. Lahore). Only those dogs which had high prevalence in Toxocara canis were examined by different faecal examination methods and were selected to get mature worms, after killing the dogs. Preservation and clearing of worms were carried out by adopting the prescribed methods. The taxonomy of the species of the genus Toxocara was studied with the help of morphological keys. The collective over all prevalence of different types of Toxocara canis recorded in stray and pet dogs was 39 5% The species of Toxocara identified in our area were: Toxocara canis, and Toxocara Leonina. Blood of infected dogs were exposed to various haematological studies including Fib estimation, erythrocyte sedimentation rate (ESR), packed cell volume (PCV), total erythrocyte count (TEC), total leukocyte count (TLC) and differential leukocyte count (DLC). Haematological examinations were made to compare the blood picture of healthy and infected dogs. For this purpose , blood samples each from healthy and infected dogs were examined. The results of haematological examination showed a significant decrease in haemoglobin estimastion, total erythrocyte count, packed cell volume Whereas a significant increase in erythrocyte sedimentation rate total leukocyte count and differential leukocyte count , like neutrophils , eosinophils and very little increase in basophils was also seen. Availability: Items available for loan: UVAS Library [Call number: 0621,T] (1).

3. Comparative Immune Response Of Anticoccidial Monovalent And Polyvalent Vaccines On Nd Vaccinated Broiler

by Wajiha Maqsood | Dr.Haji Ahmad Hashmi | Dr.Muhammad | Dr.Muhammad Afzal | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 2001Dissertation note: The study was conducted to estimate the immune response of the three anticoccidial virus vaccines on NDV vaccinated broiler chicks. The vaccines used were, Eirneria tenella vaccine (ETV), Immucox and Coccivac. For this purpose a total of one hundred and five chicks were randomly divided into five groups, namely group A, B, C, D and E on day I Each group consisting of twenty one birds. All the chicks except those in group A, were occulonasally vaccinated with Newcastle disease virus vaccine using Mukteswar strain of virus on day I and 2 1 Chicks in group A were kept as non-i nfectecl, non-vaccinated controls. Group B was non-infected, hut vaccinated with NDV. group C was also vaccinated with Eimeria tenella vaccine on days 3 and 10 of age. Group D was administered with single dose of Immucox on day 5th of age and, group E was administered with Coccivac on day 6111. After vaccination weekly mean weightgains, mean oocysts per gms of faeces (OPG), and mean feed conversion ratio of chicks in all the groups were recorded. Regular sera samples were also collected from the blood obtained from birds of all the groups, after administration of second dose of NDV on day 21 of age. All the sera samples were analyzed by using Haernagglutination (HA) and Haemagglutination inhibition (HI) tests. obtained from birds of all the groups, after administration of second dose of NDV on day 21 of age. All the sea samples were analyzed by using Haemagglutination (HA) and Haemagglutination inhibition (HI) tests. It was observed that no coccidiosis could be established in chicks of groups A, B and C. However oocysts were observed in the faecal material of chicks of group D and E after one week of administration of anticoccidial vaccines (immucox and coccivac) respectively. But OPG was gradually increase after second week of vaccination and one bird in group D died due to coccidiosis, OPG count reached to a peak after second week of vaccination and one bird in group D and one in group F died due to coccidiosis. Mean weight gains per chick on day 1 was 41, 42, 40, 39 and 38 of groups A, B, C, D and F respectively. however the mean body weights were 1746, gms 1848, 1725, 1710 and 1695 gms for chicks in groups A, B, C, D and E respectively at Group B showed significant weight gain as compared to other groups and their difference of weight gain from group B was, 103, 123, 138, and 153 gms of groups A, C, D and F respectively. (P > 0.05). Feed conversion ratio of groups A. B, C, D and F was 1.85, 1.99, 1 .88, 1.77 and 1.78 respectively at the end of experiment. Group B and C showed the best FCR as compared to other groups of the same age. But statistically the differences were non-signiFicant amongst all the test groups. Morphometeric analysis of immuno modulatory organs revealed that group B showed highest organ weight and the organ body ratio as compared to other groups of same age at the end of experiment Besides group B, group C also showed the second highest of the organ body weights and the organ body ratios, as compared to other anticoccidial vaccinated groups D and E and among them group C showed the highest of organ weight and organ body ratio. Antibody analysis revealed that geometric mean titre (GMT) in the chicks of group B (NDV vaccinated control group) showed the highest value (73.3) as compared to other groups of same age at the end of experiment. However group C showed the second highest value (68.6) on 49th day. Where as other anticoccidial vaccinated groups D and E showed their GMT value as 21.1 and 22.6 respectively, and antibody titre of group A (non-infected non vaccinated control group) was zero at the end of experiment. Availability: Items available for loan: UVAS Library [Call number: 0660,T] (1).



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