Evaluation Of Inhibition Activity Of Indigenous Lactobacilli Spp. On Ammonia Emitting Bacteria
By: Fatima Sajjad (2010-VA-312)
| Dr. Muhammad Nawaz.
Contributor(s): Prof. Dr. Masood Rabbani | Dr. Sehrish Firyal.
Material type: 
BookPublisher: 2016Description: 61p.Subject(s): MicrobiologyDDC classification: 2594-T Dissertation note: Poultry is the 2nd largest industry in Pakistan which is growing at an amazing rate of more
than 10% from last few years. It provides jobs for more than 1.5 Million people. Although, it is
growing at excellent pace, it still faces many problems. One of the important problems in poultry
farming is the production of ammonia by urease producing microbes. Ammonia is health hazard
for both poultry and human. Urease producing bacteria which are the major problems in poultry
are Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae and Staphylococcus
aureus in poultry droppings. Probiotics such as Lactobacilli can inhibit the urease producing
bacteria in poultry intestine as well as environment thus mitigate the ammonia emission. Present
study was designed to isolate Lactobacilli from indigenous poultry, screen them for
antimicrobial activity against urease producing microbes and determine their effect on growth of
Proteus mirabilis during co-culture experiments. A total of 71 Lactobacilli isolated were
recovered from 20 samples of droppings (10) and caeca (10) of back-yard poultry on MRS agar
plates. Twenty seven (27) isolates demonstrated antimicrobial activity against ammonia emitting
bacteria by agar spot and well diffusion assays. Seven isolates (FSL19, FSL25, FSL39, FSL45,
FSL51, FSL63 and FSL71), having better antimicrobial activity, were selected for co-culturing
with Proteus mirabilis in nutrient broth. FSL25, FSL45, and FSL51 showed more than 2 log10
reduction of Proteus mirabilis in co-culture experiments. FSL25, FSL45, and FSL51 were
identified as Lactobacillus plantarum, Lactobacillus fermentum and Lactobacillus salivarius,
respectively by amplifying and sequencing their partial 16S rDNA. It is concluded that
Lactobacillus plantarum FSL25, Lactobacillus fermentum FSL45 and Lactobacillus salivarius FSL51 may be used to mitigate ammonia emitting bacteria in poultry environment after further
investigations.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|---|
Thesis
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UVAS Library Thesis Section | Veterinary Science | 2594-T (Browse shelf) | Available | 2594-T |
Poultry is the 2nd largest industry in Pakistan which is growing at an amazing rate of more
than 10% from last few years. It provides jobs for more than 1.5 Million people. Although, it is
growing at excellent pace, it still faces many problems. One of the important problems in poultry
farming is the production of ammonia by urease producing microbes. Ammonia is health hazard
for both poultry and human. Urease producing bacteria which are the major problems in poultry
are Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae and Staphylococcus
aureus in poultry droppings. Probiotics such as Lactobacilli can inhibit the urease producing
bacteria in poultry intestine as well as environment thus mitigate the ammonia emission. Present
study was designed to isolate Lactobacilli from indigenous poultry, screen them for
antimicrobial activity against urease producing microbes and determine their effect on growth of
Proteus mirabilis during co-culture experiments. A total of 71 Lactobacilli isolated were
recovered from 20 samples of droppings (10) and caeca (10) of back-yard poultry on MRS agar
plates. Twenty seven (27) isolates demonstrated antimicrobial activity against ammonia emitting
bacteria by agar spot and well diffusion assays. Seven isolates (FSL19, FSL25, FSL39, FSL45,
FSL51, FSL63 and FSL71), having better antimicrobial activity, were selected for co-culturing
with Proteus mirabilis in nutrient broth. FSL25, FSL45, and FSL51 showed more than 2 log10
reduction of Proteus mirabilis in co-culture experiments. FSL25, FSL45, and FSL51 were
identified as Lactobacillus plantarum, Lactobacillus fermentum and Lactobacillus salivarius,
respectively by amplifying and sequencing their partial 16S rDNA. It is concluded that
Lactobacillus plantarum FSL25, Lactobacillus fermentum FSL45 and Lactobacillus salivarius FSL51 may be used to mitigate ammonia emitting bacteria in poultry environment after further
investigations.
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