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Inter-Relationship Of Haemagglutination Inhibition (Hi) Titres Against Newcastle Disease Vaccine (Mukteswar Straom) Of Dam's Serum, Egg Yolk And Serum Of The Chick

by Masood ul Hasan Javed | Dr. Mohammad Naeem | Dr. Sheikh Mohammad Amin | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 1992Dissertation note: The studies were conducted to determine the inter-relationship of HI titres against Mukteswar strain of NDV in the serum of dam, egg yolk and serum of day-old chick. These studies, helped not only to find out an agreement between the results of HI test of dam, egg yolk and day-old chick, but also for the suitability of the egg yolk for monitoring the immune stauts of laying flock. Four spaced injections of NDV vaccine were given to the experimental birds. The birds which did not receive 4th vaccination were referred as un-vaccinated control. At 21st day post-vaccination1 the antibody titre in yolk was lower than serum but from third week onward, comparable results were obtained with both the samples. Haemagglutination inhibition titre of dam's serum was comparatively stable throughout the experiment where as HI titre of egg yolk slightly fluctuated. Yolk, pepared by chloroform extraction and low-speed centrifugation, performed well in HI test for NOV. Maternal antibody level in day-old chick was related to the titre in dam and egg yolk. The GMT-HI titre of day-old chick fluctuated nearly in the same pattern as GMT-HI titre of egg yolk. Overall it was observed that both egg yolk and day-old chick serum HI antibody titres were slightly lower or equal to dam's serum HI antibody titre and a definite inter- relationship is present among HI titres of dam's serum, egg yolk and day-old chick. The results of this study also proved the suitabilty of egg yolk for HI antibody determination to NOV. Availability: Items available for loan: UVAS Library [Call number: 0363,T] (1).

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Bioconversion Of Industrial Wastes To 6-Aminopencillanic Acid With Escherichia Coli.

by Hasan Javed | Ms. Shagufta Saeed | Dr. Abu Saeed Hashmi | Dr. Muhammad.

Material type: book Book; Format: print Publisher: 2013Dissertation note: 6-aminopenicillanic acid is ?-lactam nucleus produced by penicillin acylaseupon hydrolysis of penicillin. 6-APA is main component of semi-synthetic penicillins. Penicillin acylase is most valuable enzyme and is produced by many microbes such as Escherichia coli. Different media and method were used for the isolation, identification an characterization of E. coli. Total 30 strains of E. coli were isolated from fecal matter of equine species and tested for the penicillin acylase activity. About 13 isolates gave the enzyme activity. For the production of cell mass, different low cost media was used to cut down the price of production. Corn steep liquor, molasses, milk whey and wheat bran was tested for the growth of E. coli. These industrial wastes can minimize the production cost of 6-APA which has a high demand for the production of semi-synthetic penicillins. Corn steep liquor showed better growth of E. coli and can be used as the cheap source of carbon and nitrogen.Phenylacetic acid was also used in the growth medium and it was used as the inducer for enzyme. Without phenylacetic acid in medium, enzyme production decreases. Corn steep liquor is the best sources for production of cells which is 0.520 mg mL-1 Molasses also better for fermentation and highest value is 0.336 mg mL-1. Milk whey media needs further studies for the better production of cells with using different concentrations.it gave best production 0.112 mg mL-1 Wheat bran is not proper source for cell production and does no showed E. Coli growth. All the strains showed growth in corn steep liquor, milk whey and molasses but not in wheat bran. Among all the strains horse sample (Ho-9) showed better cell production in all the media used. Availability: Items available for loan: UVAS Library [Call number: 1571,T] (1).

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