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1. Immunomodulatory Effects Of Vitamins On Stressed Broiler Chickens

by Isma Nazli Bashir | Muhammed Akram Muneer | Muhammed | Muhammed Amin Sheikh | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1993Dissertation note: The birds which received vitamin C (300 mg/kg of feed) had higher mean body weight than the birds which received vitamins B2, B6, 1312, cyclophosphamide and ration without vitamins. Vitamins B2, B6, B12 and C treatments did not affect the bursa of Fabricius, spleen, thymus or liver weights. Treatment of birds with cyclophosphamide in early life resulted in bursal atrophy and depression of splenic weights. Compared to Cyclophosphamide treated and ration without vitamins offered bird, the sera of NDV vaccinated birds kept on vitamins B2, B6, B12 and C had higher antibody titres on day 42. Amongst various treatment groups the highest haemagglutination-inhibition titre was recorded in Vitamin C treated birds. The post-challenge sera from NDV vaccinated birds [Group 1(vitmin B2 treated), II(vitamin B6 treated), III(vitamin B12 treated) & IV(vitamin C treated)] had higher antibody titre as compared to group V(cyclophosphamide injected) and VII(offered ration containing vitamins). The NDV vaccinated birds of groups Ib(vitaniin B2 treated), llb(vitamin &B6 treated), IIIb(vitamin B12 treated), IVb(vitamin C treated), VIb(offered ration containing vitamins) and VIIb(offered ration without vitamins) did not have any significant post virulent NDV challenge mortality. However, hundred per cent mortality in the birds of subgroups Ia(vitamin B2 treated), IIa(vitamin B6 treated), Availability: Items available for loan: UVAS Library [Call number: 0327,T] (1).

2. Molecular Detection And Speciation Of The Canme Piropiasm

by Isma Nazli Bashir | Prof. Dr.Zrafar Iqbal Ch | Dr.Peter J.Irwin | Prof. Dr. Azhar Maqbool | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 2008Dissertation note: An epidemiological study of babesiosis in dogs was conducted at Pet center, University of Veterinary and Animal Sciences, Lahore, for one year and information on age, sex and breed was gathered. It was found that from a total number of 6204, dogs up to two years of age were more susceptible than other age groups (2-4, 4-6 and above 6 years).The data regarding genders revealed that males were more prone to the disease than female dogs. As far as the breeds were concerned, crossbred dogs were more susceptible followed by Pointers, Alsatians, German shepherds and Bull terriors.Hot and humid months (June to September) have greater impact on the occurrence of disease. The study regarding identification of ticks revealed that Rhiphicephalus sanguinus is the predominant vector of the disease in Pakistan. Molecular studies were conducted to characterize and identify the species responsible for canine babesiosis in Pakistan. In this regard, a nested polymerase chain reaction-Restriction fragment length polymorphism (PCR-RFLP) technique was employed on different specimens (Blood, Body tissues and Ticks). For this purpose blood samples were collected from twenty four chronically infected dogs and applied on the Flinders Technologies Associates (FTA) cards for transportation to Australia. Different body tissues (Liver, Spleen, Kidney, Intestine, Bone marrow and Pancreas) were procured after euthanizing the two dogs and DNA was extracted, for further studies. Similarly, the eighty eight ticks were also collected from the infested dogs in the 70% ethanol for transportation to Australia. A nested PCR-RFLP assay was used for the detection and differentiation of Piroplasm species on the basis of the 1 8S ribosomal RNA gene. The assay potentially amplified and identified Babesia gibsoni as the main canine piroplasm. Similar assays on the DNA extracted from body tissues and ticks revealed Babesia gibsoni as the main piroplasm. The PCR was found to have a high detection limit (equivalent to i0 dilution), when using the DNA extracted from blood applied to FTA cards, body tissues and ticks. A new technique was developed for extraction of DNA from FTA cards and tick, in this technique, instead of using the FTA specified punching machine, we used scalpel blades, and so the rest of the chemicals used are'generally and easily available. The same protocol was used for extraction of DNA from ticks, only chemicals used in different quantities with different spinning times. Both of which, resulted in cost reduction, less effort and speedy DNA extraction. The technique reported here has the potential to be standardized for routine DNA extractions from FTA cards and ticks. Availability: Items available for loan: UVAS Library [Call number: 1061,T] (1).

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