A Profile Of Serum Albumin, Globulin Ratio, Total And Differential Leukocytic Count In 6-10 Months Old Buffalo Calves
By: Khalid Hameed, Chughtai
| Dr. M. Athar Khan.
Contributor(s): Dr. Muhammad | Dr. Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1993Subject(s): Department of Clinical Medicine & SurgeryDDC classification: 0379,T Dissertation note: One hundred arid fifty samples of each coagulated and non- coagulated blood were collected from buffalo calves (100 healthy and 50 diseased buffalo calves) of 6 to 10 months of age
The samples were analysed for total serum protein, serum albumin, serum globulin and total leuckocytic count by applying Biuret method with commercial kit (Proti) using a spectrophotorneter, and standard techninques of Cole for total leuckocytic count.
The mean total serum protein values for healthy buffalo calves of 6 to 10 months of age were observed between 5.4 19 to 7.468 g/dl. A gradual increase in totalserum protein levels was observed with an increase of age. The total serum protein values for diseased buffalo calves varied as compared to healthy calves. A decrease in mean total serum protein levels. (6.189) was associated with malnutrition, diarrhoea, fever and Fasciollosis as compared to the mean total serum protein levels in healthy calves (6.404). An increase in total serum protein level (5.793 g/dl and 7.576 g/dl) were observed during 6th and 10th month of age as compared to healthy calves (5.419 g/dl and 7.468 g/dl). This increase was due to dehydration.
A gradual increase in mean serum Albumin levels 2.458 to 3.449 g/dl was observed in healthy buffalo calves of 6 to 10 months of age. A significant (P < 0.05) difference in serum albumin values was observed in all age groups both in healthy and diseased buffalo calves. A decrease in serum albumin levels (2.516) was observed In calves suffering from malnutrition, diarrhoea, fever and fasciollosis.
The mean serum globulin levels observed in healthy buffalo calves of 6-10 months of age was observed between 3.218 to 4.144 g/dl. These serum globulin levels showed a gradual increase with increase in age of buffalo calves. An increase in serum globulin level (3.898) was observed in diseased buffalo calves as compared to healthy calves in all age groups. The diseased calves were saffertig from malnutrition, diarrhoea, fever and fasciollosis. A significant (P <0.05) difference in serum globulin values was observed in age group of 6th month of age in healthy and diseased buffalo calves whereas in all other groups it was non-significant. The mean total leukocytic count hveIs observed in healthy buffalo calves of 6 to 10 months of age ranged between 8.765 to 9.655x103 microliter. A gradual increase iii mean total leukocytic count (9.26) x 103 microlitre was observed with an increase of age in healthy buffalo calves of 6 to 10 months of age. An increase in total leukocytic count level (10.966) was observed in diseased buffalo calves as compared to healthy calves in all age groups. A significant (P <0.05) increase in total leukocytic count was observed in calves suffering from diarrhoea, fever and fasciollosis.
From the above mentioned facts it was concluded that these blood parameters are important tools in investigation/diagnosis of many diseases. These parameters enable us to understand and compare the leveI of humoral and cellular defensive components in a natural farm co idition in buffalo calves of 6 to 10 months of age.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0379,T (Browse shelf) | Available | 0379,T |
One hundred arid fifty samples of each coagulated and non- coagulated blood were collected from buffalo calves (100 healthy and 50 diseased buffalo calves) of 6 to 10 months of age
The samples were analysed for total serum protein, serum albumin, serum globulin and total leuckocytic count by applying Biuret method with commercial kit (Proti) using a spectrophotorneter, and standard techninques of Cole for total leuckocytic count.
The mean total serum protein values for healthy buffalo calves of 6 to 10 months of age were observed between 5.4 19 to 7.468 g/dl. A gradual increase in totalserum protein levels was observed with an increase of age. The total serum protein values for diseased buffalo calves varied as compared to healthy calves. A decrease in mean total serum protein levels. (6.189) was associated with malnutrition, diarrhoea, fever and Fasciollosis as compared to the mean total serum protein levels in healthy calves (6.404). An increase in total serum protein level (5.793 g/dl and 7.576 g/dl) were observed during 6th and 10th month of age as compared to healthy calves (5.419 g/dl and 7.468 g/dl). This increase was due to dehydration.
A gradual increase in mean serum Albumin levels 2.458 to 3.449 g/dl was observed in healthy buffalo calves of 6 to 10 months of age. A significant (P < 0.05) difference in serum albumin values was observed in all age groups both in healthy and diseased buffalo calves. A decrease in serum albumin levels (2.516) was observed In calves suffering from malnutrition, diarrhoea, fever and fasciollosis.
The mean serum globulin levels observed in healthy buffalo calves of 6-10 months of age was observed between 3.218 to 4.144 g/dl. These serum globulin levels showed a gradual increase with increase in age of buffalo calves. An increase in serum globulin level (3.898) was observed in diseased buffalo calves as compared to healthy calves in all age groups. The diseased calves were saffertig from malnutrition, diarrhoea, fever and fasciollosis. A significant (P <0.05) difference in serum globulin values was observed in age group of 6th month of age in healthy and diseased buffalo calves whereas in all other groups it was non-significant. The mean total leukocytic count hveIs observed in healthy buffalo calves of 6 to 10 months of age ranged between 8.765 to 9.655x103 microliter. A gradual increase iii mean total leukocytic count (9.26) x 103 microlitre was observed with an increase of age in healthy buffalo calves of 6 to 10 months of age. An increase in total leukocytic count level (10.966) was observed in diseased buffalo calves as compared to healthy calves in all age groups. A significant (P <0.05) increase in total leukocytic count was observed in calves suffering from diarrhoea, fever and fasciollosis.
From the above mentioned facts it was concluded that these blood parameters are important tools in investigation/diagnosis of many diseases. These parameters enable us to understand and compare the leveI of humoral and cellular defensive components in a natural farm co idition in buffalo calves of 6 to 10 months of age.
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