Evaluation Of Antimicrobial Therapy In Neonatal Sepsis
Material type: Book ; Format:
; Literary form:
Publisher: 2013 Dissertation note: Background: Sepsis is the presence of bacteria in blood. Neonatal sepsis indicates bacterial blood stream infection (BSI), such as gastroenteritis meningitis, pyelonephritis, pneumonia which results in fever in neonates (Weber et al. 2003). This study was designed to check the bacteriology and sensitivity pattern of microbes causing sepsis in neonates. In this study microbial susceptibility testing against antibiotics and pattern of resistance of microbes in septic patients was determined. It was conducted on neonates suspected from sepsis in Sir Ganga Ram Hospital Lahore, Pakistan. Hypothesis: By using blood cultures and different biochemical tests bacterial etiological agents of neonatal sepsis were determined and their sensitivity pattern and empirical therapies were evaluated. Material & Methods: Blood samples were collected from septic neonates admitted in Sir Ganga Ram Hospital keeping all necessary aseptic precautions with the assistance of trained professionals. The pathogens were isolated, identified and purified by selective culturing methods, which were subjected to active growth, during which sensitivity to different antibiotics were checked. The sensitivity was measured by area marked by the zone of inhibition, and National Committee for Clinical Laboratory Standards (NCCLS). Standard limit was a key indicator towards resistant bacteria. Statistical Analysis: The collected data was analyzed by appropriate statistical procedure. Outcome: It was designed to isolate and identify the pathogens responsible for neonatal sepsis and to see the effects of different antibiotics regimens for treatment of neonatal sepsis by evaluating the improvement in clinical condition, rate of complications of disease and incidence of death due to this fatal disease.
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Effect Of Zinc Oxide Nano Particles On Anti-Oxidant Status And Renal Function Test In Alloxan Induced Diabetic Rats
Material type: Book ; Literary form:
Publisher: 2015 Dissertation note: Diabetes mellitus is a chronic disorder that is affecting both developed and developing countries. Itis characterized by insulin resistance, hyperglycemia, oxidative stress and also leads to neuropathy, retinopathy, nephropathy, stroke, cardiovascular disease, gum infections, foot amputation, polydipsia, polyphagia, polyuria, muscular weakness, weight loss, hyperglycemia.Maintance of blood sugar is very necessary for healthy life. Some people control their sugar level by exercise and diet but some need medications.
Zn is an essential trace element and vital for many biological functions and plays a crucial role in more than 300 enzymes in the body. ZnONPs are environment friendly and best UV reflector so used in cosmetics and screen savers. Zn plays a unique role in immune system. Low levels of Zn lead to reduce and weakenedT cells.Zn has an important role in biosynthesis, storage and secretion of insulin. ZnONPs could be used in different medicines for treatment of cancer, autoimmune diseases and diabetes treatment.
Total of 25 Wistar rats were used in this study and divided in five groups(each group contain 5 rats).Non diabetic group served as negative control and diabetic group served as positive control, injected with physiological saline, and alloxan to induce diabetes respectively. Group A, B, C served as treatment groups, injected with alloxan and treated with three different doses (15mg/kg BW, 25mg/kg BW, 50mg/kg BW) respectively.Then on day 14; the blood samples were collected. Then the biochemical analysis of serum glucose, kidney function test and anti-oxidant status (catalase, MDA) was done by using commercially available Kits.The data was analyzed using one way analysis of variance(ANOVA).The group differences were compared by the Duncan multiple range test.Differences were considered significant at P<0.05.
The obtained results showedthat ZnONPs supplementation reduced the diabetes.We observed significant value change for antioxidant status (MDA, Catalase) and renal function tests (urea, uric acid, creatinine), but these changes were dose dependent.
So we can conclude thatZnONPs has the ability to reduce the blood glucose level but this reduction is dose dependent, as we increased the dose the reduction in serum glucose level was also increased. The highest dose in our study 50mg/kg BW was found more efficient for reduction in blood glucose, reduction in MDA, increased anti-oxidant defense system and reduction in serum urea, uric acid and creatinine level.
Availability: Items available for loan: UVAS Library [ Call number: 2425-T] (1).