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1. Toxonomical Study Of The Genus Trichuris And Comparative Anthelminitc Efficacy Of Fenbendazole (Panacur) Ad Albendazole (Valbazen) Against Gastro-Intestinal Nematodes in Sheep

by Dr. Muhammad Iftikhar | Mr. Asif Rabbani | Mr. M. Akram | Mr. Muhammad Afzal | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1990Dissertation note: Pakistan has a large population of livestock, which plays an important role in the economy of the country. Parasitism is a major problem in our country. Great economic losses have been attributed to the sheep population in our country due to the parasitic infections. The genus Trichuris includes some of the most important nematode species effecting the sheep, therefore a taxonomical study of the genus Trichuris was conducted in the month of September and October, 1989. For this purpose 100 guts (large intestine) of sheep slaughtered at Lahore abattoir were procured and brought to the parasitology laboratory College of Veterinary Sciences, for further investigations. The overall incidence of Trichuris spp. was found to be 74% . The following two species were recorded. 1. Trichuris ovis 66% 2. Trichuris globulosa 18% Taxonomy of Trichuris species was studied by preparing permanent mounts. The present work was also aimed to see the effect of two commonly available anthelmintics namely fenbendazole (panacur) and albendazole (valbazen), on naturally infected sheep with gastrointestinal nematodes. Sixty sheep positive for nematode infections were randomly divided into three groups A,B & C each group comprising of 20 animals. Group A and B were given fenbendazole and albendazole respectively according to the manufacturers dose rate while group C was kept as untreated control. The efficacy of both the drugs was evaluated on the bases of reduction of eggs per gram of faeces after medication. The egg counts were made on zero, 7th, 14th and 21st day. Both the drugs revealed a progressive decrease in the faecal egg counts. The efficacy of fenbendazole on 7th, .14th and 21st day was 96.33, 97.05 and 99.66% respectively while the efficacy of albendazole was 92.56, 95.37 and 98.18% respectively. There was no significant difference in the effectiveness of the drugs. Availability: Items available for loan: UVAS Library [Call number: 1130,T] (1).

2. Dna Fingerprinting Of Pakistani Buffalo Breeds (Nili-Ravi, Kundi) Using Microsatellite And Cytochrome B Gene

by Rashid Saif | Prof.Dr.Masroor Elahi Babar | Mr. Asif | Prof. Dr. Abu Saeed Hashmi.

Material type: book Book; Format: print Publisher: 2010Dissertation note: Customarily, classification of breed was based on phenotypic traits. In some cases, recent genetic studies have found differences in the structure proposed. In buffalo ,one has to bear in mind that morphological changes were not the result of adaptation to the environment, but have a social significance thus may not be indicative of the genetic relationship. In recent years microsatellites have proved to be very useful for the determination of genetic relationship among population. Comparative studies beiween microsatellite and protein markers have highlighted the advantages of the former. The water buffalo (Bubalus bubalis) holds tremendous potential in livestock sector in many Asian countries, particularly in Pakistan but the genetic data of different buffalo breeds like Nili-Ravi and Kundi is lacking, which need to be established for their genetic identification. Blood samples of unrelated true representative of both breeds (Nili-Ravi and Kundi) were collected from different government livestock farms in Punjab and Sindh respectively. DNA was extracted by inorganic method and amplification of the mitochondrial Cytb gene and microsatellite was done with especially designed primers in Molecular Biology and Genomics Laboratory at Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore. Cytochrome b gene markers have been proved as an efficient and powerful tool for breed characterization and species identification of buffaloes. Several panels of microsatellite markers have also been reported for this purpose. In this study, a panel of nine microsatellite markers has highly Polymorphism Information Content (PlC) were selected, Specific primers were designed for these microsatellite and Cytb gene partial amplification using primer3 software. Then primers were optimized for successful amplification with minimum reagent concentration. PCRs were performed for amplification of these microsatellite and Cytb markers on each sample, Genotyping and sequencing was conducted on all amplicons to find out the different SNP to design haplotypes with the help of bioinformatics software e.g. Blast 2sequence and Chrornas Lite, Further statistical analysis was done by the help of some other software e.g. Popgene version 1.31, Power Stat., Genetic diversity, Allele frequencies, observed and expected homozygosity and heterozygosity, Hardy Weinberg equilibrium, F-Statistics and Gene Flow for all Loci, population's dendogram, Nei's genetic identity and genetic distance! diversity was calculated. The results obtained from this study can contribute to the establishment of routine DNA typing services, beneficial for the buffalo industry as well as in animal forensics for litigation and expedite the police investigation services in Pakistan, which will also be useful for breed characterization and phylogenetic study of aforementioned breeds of buffalo. Availability: Items available for loan: UVAS Library [Call number: 1183,T] (1).

3. Dominant Inheritance Of Myopia Linked Loci Myp10 And Myp11

by Saira Nabi | Mr. Asif Nadeem | Mr. Muhammad Imran | Prof.

Material type: book Book; Format: print Publisher: 2010Dissertation note: Myopia, a refractive error is one of the most common ocular disorders worldwide with elongation of axis of the eyeball. Genetic plays an important role in the development of myopia. Familial myopia is common in Pakistan. The aim of the study was to find out the dominant inheritance through linkage analysis and molecular characterization of Myopia in Pakistani myopic families and also to determine the presence of loci (MYP 10 and MYP1 1). Six families with three or more affected individuals in two or more ioops were enrolled from different areas of Lahore. The persons with refractive error equal or worse than -l were considered as myopic. Out of the total 36 samples, 26 were myopic. For the each locus, 3 markers were designed .Polyacrylamide Gel electrophoresis (PAGE) was used for genotyping of amplified DNA samples. Haplotypes of all the families were constructed based on the PAGE results to check weather a family is linked or unlinked to those loci. Result of allele haplotyping were analysed to evaluate the linkage of Myopia loci and dominant inheritance in Pakistani families. No linkage was found for any of those selected families. The negative results for these chromosomal regions have several possible reasons; sample size, family size and ethnicity of the families are the major reasons for these negative results. Although there was no linkage for the loci MYP10 and MYP11, this would be the first molecular investigation of the Myopia loci MYP10 and MYP11 in Pakistani families. The findings of the proposed study will be vital for victim familes in terms of genetic testing, genetic counseling, for designing a management plan and resource alloction for victims. Availability: Items available for loan: UVAS Library [Call number: 1215,T] (1).

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