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Lysine Production On Pilot Scale By Brevibacterium Flavum And Its Characterization, Purification And Crystallization

by Muhammad Faisal | Dr. Abu Saeed Hashmi | DR. Aftab | Mrs. Shagufta Saeed.

Material type: book Book; Format: print Publisher: 2013Dissertation note: Food and feed protein demands have increased due to raise in population. Therefore continuous efforts have been progressed to enhance the production rate by conventional and non conventional methods. Fermentation technology have participated decisive role for a long time period and presently the amino acids formed by fermentation set apart principal biotechnology products significantly. By consuming low-cost carbon supply mutants originate potential to the inexpensive built-up for amino acids. L-lysine demand is steadily rising in the sector of feed stuffs, soft drinks, food ingredients, pharmacy and biological fluids, etc. In order to meet the market demand and accomplish growing and assorted L-lysine requirements, microbial metabolic engineering and recombinant DNA technology is the only hope and possibility for advancing the strains. Purification and isolation of material produced is a very significant element extremely influences fermentation practice usefulness and manufacturing expenses. It demands enhancement in the recycling procedure of amino acids, mainly L-lysine. The present study was designed to produce lysine on pilot scale by using Brevibacterium flavum. A variety of agricultural byproducts like wheat bran, sugar cane molasses and rice polishing were utilized as substrate for lysine production through fermentation by using Brevibacterium flavum. Primarily optimum conditions were determined through fermentation for lysine production on micro scale. Subsequently these conditions were employed for biosynthesis of lysine on pilot scale. Qualitative assay of lysine was performed by TLC and quantitative assay by spectrophotometrically. It was found that amongst all the substrates 4% molasses was produced maximum lysine at 300C. Different inorganic and organic material like 0.4% CaCO3, O.4% MgSO4.7H2O, 0.1% NaCl, 0.8% KH2PO4, 2.5 % (NH4)2SO4, 0.5 % urea, 0.04 mg % biotin and 0.6 % corn steep liquor were found to be optimal for maximum lysine yield. After pilot scale production of lysine in fermentor, different techniques of downstream were applied. The biomass liquor thus produced was purified and crystallized through different techniques to transform in to L-lysine crystals. The information thus attained was subjected to statistical analysis by using one way ANOVA on optimization of different parameters for L-lysine production and comparison of mean values was done by Least Significant Difference (LSD). Based on the above observations it was concluded that molasses is the most suitable substrate among other agriculture wastes for maximum lysine production with Brevibacterium flavum. Availability: Items available for loan: UVAS Library [Call number: 1631,T] (1).

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2.
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Identification Of Multiple Drug Resistant (Mrd) Mastitis Causing Bacteria In Dairy Goats

by Muhammad Faisal najees | Dr. Aftab ahmad anjum | Prof. Dr. mansur-ud-Din ahmad.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1852,T] (1).

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Study Of Prescribing Trends And Evaluation Of Therapeutic Effectiveness Of Prescribed Medications For Type 2 Diabetes Mellitus In Tertiary Care Hospital Of Lahore, Pakistan

by Bareera Rana (2008-VA-545) | Allah Bukhsh Awan | Muhammad Faisal Nadeem | Dr. Muhammad Ovais Omer.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Diabetes mellitus is metabolic disorder resulting from decrease in insulin secretion and insulin action or both. Presently, the prevalence of diabetes is 8.3% and 7.89% in world and in Pakistan, respectively. Pakistan is ranked 7th in diabetes mellitus prevalence making it a major problem in our health care system. The study was based on the prescribing trends and evaluation of therapeutic effectiveness of oral hypoglycemic agents in tertiary care hospital of Lahore. A concurrent observational study of 3 months duration was performed in diabetes management centre of a tertiary care hospital of Lahore under the supervision of an endocrinologist. Socio-demographic data and compliance levels were determined by questionnaire while hyperglycemic control was analyzed by continuous monitoring of HbA1c at the start and the end of the study. Data was finally analyzed by descriptive statistics and SPSS 16. The study demonstrated that the most frequently prescribed oral hypoglycemic agent was metformin which is in accordance with the guidelines, however individualization of treatment is required keeping in view the patient factors respectively. Intensification of current treatment for patients with higher HbA1c values is needed. Data regarding therapeutic effectiveness of the hypoglycemic agents shows that the hypoglycemic agents were effective enough to lower HbA1c values to bring it to normal values but patient factors of non-compliance and not following life style modification patterns are not allowing the values to come up to the mark, that is, the decrease in HbA1c values according to guidelines within three months of the treatment. Presently it is the need of time to run educational awareness programs for awareness regarding diabetes among people to made them know about life style interventions. Moreover the prescribing trends and their relevance with glycemic control must also be documented. It will generate data for knowing which trend of prescribing is producing better results and whether the trends are meeting international standards or not. Availability: Items available for loan: UVAS Library [Call number: 2427-T] (1).

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In-Vivo Efficacy Of Aqueous And Alcoholic Extracts Of Hedera Helix Against Fasciolosis In Sheep

by Muhammad Faisal Raza (2008-VA-206) | Dr. Muhammad Avais | Dr. Muhammad Hassan Saleem | Dr. Imran Rashid.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: The liver fluke, Fasciola hepatica, is a common parasite of ruminants in many countries in the temperate climates and often causes severe economic losses. Infection with both F. hepatica and F. gigantica may also occur in occasionally causing large scale epidemics. These parasites adversely affect the health status of animals and cause enormous economic losses Triclabendazole (TCBZ) is a worldwide, one of the most used drugs for the control of fasciolosis but the efficacy may depend on the susceptibility of the strain involved in the drug. However, resistance of the liver fluke to TCBZ in naturally infected sheep has been reported. Plants are being used as anthelmintic as the allopathic drugs today which offer substitute to the use of synthetic chemicals. This study was carried out to evaluate the efficacy of aqueous and methanolic extracts of H. helix against fasciolosis in sheep. For this purpose, adult sheep was used for In vivo trials. Animals were screened for the presence of liver fluke and 30 sheep positive for liver fluke infestation were used for therapeutic trials. They were divided into 5 groups. Group A and B were given single dose of aqueous extract of Hedera helix at concentrations of 1.13 gm/kg and 2.25 gm/kg body weight respectively. Animals in C and D group were given 1.13 gm/kg and 2.25 gm/kg body weight respectively, while sheep in E group were given Triclabendazole at dose rate of 1 ml/5kg body weight. Fecal samples from each animal was collected in the morning, starting from day 0 pre-treatment and at days 4,7 and 14 post-treatment and were evaluated for the EPG through Modified McMaster method. The comparative efficacy was analysed by two way analysis of variance (ANOVA) using SPSS 20.0. A probability levels ˂0.05 was considered as statistically significant. During In vivo experiment all the treatments showed significant reduction in EPG. The maximum reduction in EPG showed by crude aqueous extract (CAE) at day 4 post treatment at 1.13 gm/kg dose rate was 20.0012 %. There were no significant reduction in EPG at day 7 and 14 day for aqueous extract. The maximum reduction by aqueous extract at day 4 at dose Summary 29 rate of 2.25 gm/kg was 40.47 % and at day 7 it was 45.24 %, whereas there was no significant reduction at day 14 post treatment. The maximum reduction showed by crude Methanolic extract at dose rate of 1.13 gm/kg at day 4 was 29.54 %. At day 7 post treatment the reduction of EPG was 40.09 % and at day 14 the reduction was 43.18 %. The maximum reduction showed by crude Methanolic extract at dose rate of 2.25 gm/kg at day 4 was 56.09 %. At day 7 post treatment the reduction of EPG was 64.85 % and at day 14 the reduction was 68.29 %. The positive control treated with Triclabendazole showed the maximum reduction of 97.5 % in eggs per gram (EPG). So it is concluded from the study that the extracts of Hedera helix have the anthelmintic efficacy against liver fluke. The increase in dose rate of extracts may give better results. So there is need to conduct further studied and more trials on Hedera helix extracts under field conditions to achieve maximum efficiency levels. Availability: Items available for loan: UVAS Library [Call number: 2431-T] (1).

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Efficacy Of Different Microbial Phytases On Growth Performance Carcass Characteristics And Mineral Retention In Broilers

by Muhammad Faisal Rafique (2014-VA-918) | Dr. Talat Naseer Pasha | Dr. Saima | Dr. Athar Mahmud.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Cd Crupt Availability: Items available for loan: UVAS Library [Call number: 2762-T] (1).

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