1.
Immunohistochemical And Pathomorphological Studies Of Chronic Granulomatous Enteritis (John'S Disease) in Bovines
by Muhammad Shahid | Prof.Dr.Zafar Iqbal Ch | Dr.Asim Aslam | Prof.Dr.Khushi Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2010Dissertation note: Paratuberculosis, a disease caused by Mycobacterium paratuberculosis is a peril for both livestock and human beings. The present project was designed to study the pathmorphological changes induced by the organism and standardize more reliable diagnostic techniques to identify the M paratuberculosis. Tissue samples from ileurn and mesenteric lymph nodes were randomly collected from 1 50 cattle and buffalo, each in present study that was conducted in Lahore.
Gross lesions were recorded on a Performa. The samples were subjected to acid fast staining of smears from pellets after density gradient centrifugation and paraffin embedded tissue sections. All the samples also subjected to polymerase chain reaction and immunohistochemistry. The smears prepared from bacterial pellets of mucosal and cortical scraping of terminal ileum and MLN were stained indicated 11.4 % small intestine and 12.7% lymph nodes of cattle's and 8.7% and 10.7% lymph nodes of buffalo's tissue samples were positive. ZN staining of paraffin embedded tissue showed 8.0 % small intestine and 10% MLN of cattle's and 6.0 % of small intestine and 8.7% MLN in buffalo's tissue samples were positive. On basis of PCR 5.4% intestinal tissue samples and 6.0% MLN of cattle were positive. 3.4% intestinal tissue samples and 07(4.7%) MLN of buffaloes were positive. In buffaloes 4.0% intestinal tissue samples and 6.0% MLN were positive by IHC. In cattle 6.7% intestinal tissue samples and 8.0% MLN tissue samples were positive by IHC. In cattle, 27/150(18.0%) animals showed lesions in both intestine and mesenteric lymph nodes while 5/32 (15.7%) animals showed lesions in lymph nodes only. Out of 27/150(18.0%) intestinal tissue samples, 20/27 (74.1%) samples showed corrugation of the intestinal mucosa while 7/27 (26%) showed diffuse thickness.
In buffalo, 24/150 (16.0%) animals showed lesion in both intestine and mesenteric lymph nodes while 2/26 (7.7%) animals showed lesion in lymph nodes only. Out of 24 intestinal tissue samples, 19/24(79.2%) with gross lesion, samples showed corrugation of the intestinal mucosa while 5/24(20.9%) showed diffuse thickness.
In histopathology 20/27 samples of cattle showed focal granulomatous lesions while 7/27(26%) samples showed sever infiltration of macrophages and lymphocytes while 28/32(87.5%) lymph nodes showed infiltration of paracortical and cortical region of the lymph nodes with macrophages ,lymphocytes and multinucleated giant cells While 4/32 (12.5%) samples showed moderate infiltration of macrophages.
In buffaloes 19/24 (12.7%) samples showed focal granulomatous lesions while 5/24 (20.9%) samples showed sever infiltration of macrophages and lymphocytes while 22/26 (84.7%) lymph nodes showed infiltration of paracortical and cortical region of the lymph nodes with macrophages ,lymphocytes and multinucleated giant cells While 4/26 (15.4%) samples showed moderate infiltration of macrophages.
The sensitivity and specificity of immunohistochemical method was found significant in comparison Ziehl-Neelsen staining and histopathology for the diagnosis of paratuberculosis in cattle and buffaloes.
Availability: Items available for loan: UVAS Library [Call number: 1078,T] (1).
2.
Prevalence Of Cestodes And Comparative Efficacy Of Niclosamide, Mebendazole And Fenbendazole In Pet Dogs
by Muhammad Shahid | Dr. Asim Khalid Mahmood | Prof. Dr. Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2010Dissertation note: This study was conducted to assess the prevalence of cestodes infestation in pet dogs in district Lahore and to evaluate the efficacy of niclosamide, mebendazole and fenbendazole against them. For this the samples were collected from three different clinics in Lahore for a period of 3 months. A total of 400 samples were collected. Three types of cestodes were identified (E.granulosus, T.hydatigena and D.caninum). The highest prevalence revealed was that of T.hydatigena and the lowest was of D.caninum in pet dogs. For the drug trials, 32 dogs were divided into four groups named group A (niclosamide), group B (mebendazole), group C (fenbendazole) and group D (positive control). The sampling was performed on day 0, 7 and 21 & the treatment with each drug was made on 0 and 7 days. Later on the reduction in EPG and the calculation of efficacy revealed that mebendazole is the best drug among the three drugs used and the efficacy of fenbendazole was found to be very poor.
Availability: Items available for loan: UVAS Library [Call number: 1164,T] (1).
3.
Study On Autologous And Homologous Platelet Rich Plasma As A Surgical Wound Healing Promoter In Rabbits
by Muhammad Shahid Tahir (2012-VA-996) | Dr. Uzma Farid Durrani | Dr. Asim Khalid Mahmood | Prof. Dr. Muhammad Sarwar Khan.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Wound healing is a complex process in which tissue repairs itself with the complete or incomplete regeneration. During recent years, new advances have been employed in the monarchy of tissue healing by initiation of use of platelet rich plasma (PRP) as a wound healing promoter in both soft and hard tissue surgeries. Platelet rich plasma is highly potentiated with growth and healing factors that can lead to marked acceleration in the tissue adaptation, repair and healing as compared to PRP non-facilitated healing process. This study was conducted on 16 adult healthy rabbits contributing 2 different groups, each group with 8 rabbits. Rabbits of both groups were surgically operated for skin autogenous grafting. Group A received an application of autologous PRP subcutaneously and in the surroundings of the graft defect, while group B received homologous PRP. Efficacy of both autologous and homologous PRP was evaluated on the basis of selected macroscopic and microscopic (histopathological) parameters of tissue repair and healing. The results, obtained in form of grades, were statistically analyzed by graphical representations. A successful use of autologous and homologous PRP with the desired results did not only boost up the surgical success rate in the specie under study but it opened a new vista on the same lines for other species to bring a better wound healing process. This study uprooted the conventional methods of managing wounds associated with injuries and helped the animals to get confidently satisfactory health status. Availability: Items available for loan: UVAS Library [Call number: 2452-T] (1).
4.
Sapah Silar e Azam (PBUH) Waqiat e Gazwat
by Muhammad Shahid.
Edition: 1stMaterial type: ; Format:
print
; Literary form:
not fiction
Publisher: Lahore: Ilam o Dost Publications ndAvailability: Items available for loan: UVAS Library [Call number: 297.63 Shahid 21198 1st nd Islam] (1).
5.
Anthelmintic Activity Of Ginger Against Gastrointestinal Nematodes In Goats
by Muhammad Shahid (2008-VA-127) | Dr. Haroon Akbar | Prof. Dr. Kamran Ashraf | Dr. Muhammad Avais.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Gastrointestinal tract nematodes are responsible for wide range of health problems,
economic losses in goats and are characterized by impaired milk production, meat process,
decreased fertility, low kidding rates, decreased working efficiency and even death of the goats.
Gastrointestinal tract nematodes cause economic losses via morbidity and negative effects on
feed intake, nutrient utilization efficacy and also reduce young animal’s growth rate as a result,
leading to decreased productivity and performance of the infected animal. Due to such economic
losses, the control of the helminths is unavoidable which is also possible by herbal products such
as Ginger. Ginger has pharmacological and gastrointestinal prokinetic activities to cure
constipation, indigestion, vomiting, infectious diseases and helminthiasis. In the current study,
anthelmintic activity of Ginger has been tested against gastrointestinal nematodes of goats.
For therapeutic trials, a total of 75 goats positive for nematodes having EPG >150 were
selected randomly and divided into three groups named as Group A, B and C, each group
comprising of 25 animals. The goats of Group A were orally treated with crude powder of ginger
(Zingiber officinale) @ 3 gram/kg body weight, orally. Goats in group B served as positive
control (infected and treated with Oxfendazole). Group C comprised of positive animals which
were not treated during whole the experiment. The fecal samples were collected at day 14 (Posttreatment).
Drug’s efficacy was assessed on the Fecal Egg Count Reduction Test (FECRT) and
was calculated by following formula (Traversa et al. 2007).
[((Pre-treatment EPG – Post-treatment EPG)) / Pre-treatment EPG] × 100
SUMMARY
34
Data regarding therapeutic trails were analyzed by repeated measures one-way ANOVA, using
SPSS version 20.0, p< 0.05 was considered as significant. Trial was analyzed under different
parameters.
Ginger has shown good anthelmintic activity against gastrointestinal nematodes in goats
as evident from 63% reduction in EPG. It is suggested hereby to conduct a dose trial for the use
of ginger against nematodes in goats by using different dose levels including at least 5 different
groups of dosages like 3gram; 3.5grams; 4grams; 4.5grams and 5grams per Kg body weight. The
current study has highlighted the anthelmintic activity of ginger (Zingiber officinale) against
gastrointestinal nematodes in goats. More trials using this herbal product in other animals will
further highlight the importance of using this commonly-available and economical herbal
product in the control of gastrointestinal nematodes in livestock. Availability: Items available for loan: UVAS Library [Call number: 2712-T] (1).
6.
A Comparative Study Of Adulteration In Raw Milk Samples Collected From Rural Farmers And Milk Sold In Urban Areas Of Sahiwal Region
by Muhammad Shahid (2008-VA-418) | Dr. Sanaullah Iqbal | Dr. Waqas Ahmed | Dr. Muhammad Nadeem.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Milk is the rich source of all nutrients when compared with other food items. Milk
procurement, collection and marketing system in Pakistan is quite old and informal markets
always involve milk sale through unregulated channels. Due to the large numbers of
uneducated and non-technical smallholding farmers, milk handling processes in the
traditional system are extremely unhygienic and there is insufficient enforcement of
standards, resulting in adulteration and at the end poor quality products. Adulterated milk is
selling in Sahiwal region and this study helped to identify milk adulteration in milk supply
chain during milk collection, transportation and selling at shops.
This comparative study has two phases. In first phase, total 80 milk samples were
collected from farmers in rural areas of Sahiwal region. All samples were analyzed to detect
adulterants (Starch, Urea, Hydrogen peroxide, Detergent/Soap, Sorbitol, Boric Acid, Cane
Sugar, Sodium Chloride, Carbonates, Formalin, QAC and Hypochlorite) in raw milk
produced by local farmers. In the second phase, total 60 milk samples were collected from
different shops located in urban areas of Sahiwal region. All samples were analyzed to detect
adulterants (Starch, Urea, H2O2, Detergent/Soap, Sorbitol, Boric Acid, Cane Sugar, Sodium
Chloride, Carbonates, Formalin, QAC and Hypochlorite) in raw milk sold in urban areas of
the region. All milk adulteration tests were performed by using University of Veterinary and
Animal Sciences (UVAS), Quality Operation Laboratory (QOL), Milk Adulteration Testing
(MAT) kit.
Summary
41
The aim of this study was to identify sources of adulteration in raw milk collected
from rural farmers and sold at shops in urban areas of Sahiwal region.
Total 140 milk samples were collected both from rural and urban areas of Sahiwal
region. 18 (13%) milk samples out of 140 were found adulterated both from urban and rural
locations of Sahiwal region. Sixty (60) milk samples were collected from urban locations of
Sahiwal for detection of adulterants. Total 12 (20%) out of 60 samples were found
adulterated in urban locations. 80 milk samples were collected from rural locations of
Sahiwal region for detection of adulterants 6 (7.5%) out of 80 samples were found
adulterated in rural locations.
Milk adulterants were detected in both rural and urban locations of Sahiwal region.
These adulterants were present in milk samples collected from Sahiwal region urea, formalin,
sugar cane, starch, detergent, sorbitol and carbonates. Boric acid, H2O2, quaternary
ammonium compounds, hypochlorite and sodium chloride were not detected both in urban
and rural locations of Sahiwal.
The conclusion of this comparative study is that milk adulteration is higher in urban
locations (20%) of Sahiwal as compared to rural location (7.5%) of Sahiwal region. This
study also shows the district wise milk adulteration in all three districts of Sahiwal region that
are 10%, 11% & 17 % in Sahiwal, Pakpattan & Okara districts respectively. Availability: Items available for loan: UVAS Library [Call number: 2897-T] (1).
7.
Mukhtasar Tareekh e Pakistan
by Dr. Muhammad Shahid Shoukat.
Edition: 1st.Material type: ; Literary form:
not fiction
Publisher: Lahore: Dar al Shaoor; 2015Availability: Items available for loan: UVAS Library [Call number: 891.439 Shoukat 32653 1st 2015 Urdu.Literature] (1).
