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Evaluating The Efects Of Prebiotics In Rehabilitation Of Gut Microbiota After Antibiotic (Cephalexin) In Rats

By: Rabia Omer (2011-VA-512) | Dr. SanaullahIqbal.
Contributor(s): Mr. Muhammad Amir Iqbal | Dr. Sajid khan Tahir.
Material type: materialTypeLabelBookPublisher: 2017Description: 66p.Subject(s): Food Science and Human NutritionDDC classification: 2859-T Dissertation note: Antibiotics are widely used to kill pathogenic bacteria that have adverse effects on human health. It has been seen that these kind of drugs causes imbalance of microbes because these are not only target the pathogens but also suppressing the growth of beneficial bacteria. Beneficial bacteria such as bifidobacteria and lactobacilli species conquer many benefits for human health. That’s why there is a need to rehabilitate these valuable microorganisms. The worthwhile approach in this regard is the use of galacto-oligosaccharides(GOS). Adult male Wistar rats (n= 24) were used in present study. Rats were divided into 4 groups having 6 rats in each group. Group G1 was a control normal that was fed on a basal diet. Group G2 was treated with an antibiotic cephalexin for five days. Moreover, Group G3 wasgiven GOS as well as antibiotic. In Group G4, firstly antibiotic was given for specific duration and after that GOS was given for the rest period of time. The fecal sample were collected from rats with time interval of 0, 5 , 10 and 15day of antibiotic therapy or treatment and were analyzed for bacterial population especially Bifidobacteria, Lactobacilli, E. coli and total plate count using selective media. The results of bifidobacteria and lactobacilli of group 3 were significantly same with group 1 at 15th day and were notably higher from group 1 at 20th day. In addition the beneficial species in group 2 were decreased at 5th day that significantly different with group 1 and didn’t recover till at 20th day. The colonies of E. coli in various groupswere different at various time intervals. Moreover the results of TPC were based on the treatments. It was significantly higher in group 3 that was given antibiotic with prebiotics while it was significantly lower in group 2 as compared to normal.The data were analyzed using One way Analysis of variance (ANOVA). The group differences were compared by the Duncan’s Multiple Range Test. Differences were considered significant at P < 0.05. This study helped to turn down the side effects of antibiotic as well as maintain the normal micro-flora by rehabilitate the beneficial species such as bifidobacteria and lactobacilli and also suppressing the growth of harmful pathogens e.g. E. coli
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Veterinary Science 2859-T (Browse shelf) Available 2859-T
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Antibiotics are widely used to kill pathogenic bacteria that have adverse effects on human health. It has been seen that these kind of drugs causes imbalance of microbes because these are not only target the pathogens but also suppressing the growth of beneficial bacteria. Beneficial bacteria such as bifidobacteria and lactobacilli species conquer many benefits for human health. That’s why there is a need to rehabilitate these valuable microorganisms. The worthwhile approach in this regard is the use of galacto-oligosaccharides(GOS).
Adult male Wistar rats (n= 24) were used in present study. Rats were divided into 4 groups having 6 rats in each group. Group G1 was a control normal that was fed on a basal diet. Group G2 was treated with an antibiotic cephalexin for five days. Moreover, Group G3 wasgiven GOS as well as antibiotic. In Group G4, firstly antibiotic was given for specific duration and after that GOS was given for the rest period of time. The fecal sample were collected from rats with time interval of 0, 5 , 10 and 15day of antibiotic therapy or treatment and were analyzed for bacterial population especially Bifidobacteria, Lactobacilli, E. coli and total plate count using selective media.
The results of bifidobacteria and lactobacilli of group 3 were significantly same with group 1 at 15th day and were notably higher from group 1 at 20th day. In addition the beneficial species in group 2 were decreased at 5th day that significantly different with group 1 and didn’t recover till at 20th day. The colonies of E. coli in various groupswere different at various time intervals. Moreover the results of TPC were based on the treatments. It was significantly higher in group 3 that was given antibiotic with prebiotics while it was significantly lower in group 2 as compared to normal.The data were analyzed using One way Analysis of variance (ANOVA). The group differences were compared by the Duncan’s Multiple Range Test. Differences were considered significant at P < 0.05.
This study helped to turn down the side effects of antibiotic as well as maintain the normal micro-flora by rehabilitate the beneficial species such as bifidobacteria and lactobacilli and also suppressing the growth of harmful pathogens e.g. E. coli

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