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1. Standardisation Of Indirect Haemagglutination Test For Monitoring Infectious Bursal Disease Virus

by Sajid Mahmood | Dr. Muhammad Akram Muneer | Dr. Hajid | Dr. Khushi Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print ; Nature of contents: biography; Literary form: Publisher: 1997Dissertation note: Indirect haemagglutination (IHA) test was standardized and evaluated to moniter antibodies against infectious bursal disease (IBD). It was observed that oil based vaccine prepared from bursae of fäbricius of infected birds, induced a high level of antibody which were detected by agar gel precipitation test (AGPT). It was recorded Chat tannic acid, glutaraldehyde and chromium chloride had 0.0000781, 0.003906 and 0.0001562 per cent subagglutinating dilutions in normal saline solution (pH 7.2) respectively while 0.000001220, 0.0156 and 0.0025 subagglutinating dilution of the coupling agents were found in phasphate buffered saline (pH 7.2), respectively. Indirect. haemagglutination test is sensitive and specific serological technique to study infectious bursal disease. However, antigen dilution to sensitize erythrocytes, source of erythrocytes, chemical nature of diluent, interaction temperature and time, nature and concentration of coupling agent coated erythrocytes and antiserum against IBD, had influenced the sensitivity of IHA test. Ten percent antigen for sensitizing sheep erythrocytes, incubation temperature of 37°C for 10 minutes for antigen, tannic acid (0.005%) and erythroéyte interaction, freshly prepared sensitized erythrocytes and normal saline solution (pH 7.2) as diluent were found suitable for detecting maximum titre of anti-IBD antibodies through the IHA. Moreover it was observed that the standardized IHA proportionally showed reduction in the titre on dilution of serum. The antibody titre in the IHA was the well having serum dilution, showing resistance to bleed (flow) on tilting the plate for 5 seconds. The final results of antibody titre were achieved within 120 minutes post processing of the samples. Availability: Items available for loan: UVAS Library [Call number: 0529,T] (1).

2. Evaluation Of Water Hyacinth (Eichhornia Crassipes) Supplemented Diet On The Growth, Digestibility And

by Sajid Mahmood | Dr. Noor Khan | Prof. Dr. Muhammad Aahraf.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2013Dissertation note: Present experiment was conducted in 8 aquaria of size 1.5ft x 2ft x 3ft (width, height and length, respectively) with 1.5 feet water level in each aquarium on fingerlings of Ctenopharyngodon idella. Sun dried different parts of water hyacinth was used to prepare three isoproteic diets (30%) i.e. whole plant meal diet (WP), leaf meal diet (LM), root meal diet (RM) and a control diet. Overall significant (p. 0.05) difference was observed in weight and length among the treatments. On termination of experimental trial, all the fish were harvested, weighed and measured for assessment of growth performance. Representative samples from each treatment were randomly picked up for determination of body composition and histological studies. Former were dried for proximate analysis while latter were dissected, liver and kidney were excised out for further tissue processing. Statistically non-significant variations in increase in weight and length were recorded among Control, WP and LM diets while RM depressed fish growth. Mean weight 4.636}0.605g and length 78.757}3.995mm were relatively higher when grass carp juveniles were fed with LM and the same were the lowest 2.465}0.178g and 65.405}1.877mm, respectively, for RM diets. Higher weight gain was observed in LM (7.14 g) and lower on RM (2.10 g). Maximum length increase was observed on WP (50 mm) and minimum on RM (20 mm). Better FCR was observed on LM (2.113}0.102) and poor on RM (5.142}0.212). SGR % was observed maximum on LM (0.787}0.051) and minimum on RM (0.415}0.041). Histological study showed that there was no variation in liver and kidney in the Control diet whereas variations in cell patterns have been observed due to incorporation of water hyacinth which may be due to inherent toxic materials present in water hyacinth or due to some 61 heavy metals it absorbed from the surroundings. This research exposes that Eichhornia crassipes meal has an optimistic nutrient utilization effect on fish growth. Farmers can use water hyacinth to formulate cost-effective fish feeds. Water hyacinth leaf meal is more appropriate for fish production than the whole plant meal. But farmer has to be very careful and make sure that water hyacinth to be used in feed should come from clean water, because water hyacinth can absorb heavy metals and poisons from polluted water. Such plants if used in fish feed will affect the fish health and farmer profit. Availability: Items available for loan: UVAS Library [Call number: 1716,T] (1).

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