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1. Effect Of Additional Pructose On The Longevity Of Buffalo & Sahiwal Bull Semen

by Shafique, M | Not Available | Not Available.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 1970Dissertation note: The present study was conducted to determine the effect of additional fructose on the longevity of buffalo and Sahiwal bull semen. Two buffalo-bulls and two Sahiwal-bulls of 3 to 9 year of age were selected for the collection of semen. These bulls were stationed at the Artificial Insemination Section of College of Animal Husbandry, Lahore, under identical environmental conditions. A total of 16 samples of semen were collected for the four bulls. BY physical and microscopical examination, samples with four or above mass motility were selected for experiment. Heated homogenized whole-buffalo-milk was used as an extender.Fructose was added in different fractions to the milk (extender) prior to semen extension. Ten percent glycerol alongwith 0.5 mg. of Streptomycin and 500 units of Penicillin per ml. of the extender were also added to each sample. During the study, data were collected in 1:20, 1:30 fructose per 100 ml. of the extender to evaluate the effect of fructose on the longevity of semen. The data were analysed by analysis of variance, and Duncan's Multiple Range Test was also applied to determine the effect of fructose. The Studies revealed that:- 1. The semen samples with additional fructose were significantly of higher quality than that of the samples without fructose. 2. With an increase in the fructose level alongwith extension rate, there was an increase in the longevity of the spermatozoa. 3. Sahiwal bull semen could be preserved over a longer period as compared to that of buffalo bull. 4. On motility basis, 1:40 extension with 2000 mg. of additional fructose per 100 ml. of the extender could safely be used for five and six days in case of buffalo and Sahiwal bull semen respectively. Fructose treated semen is further recommended for field trials with a view to determine actual fertility percentage. 5. The fructose had a significant effect on the longevity of semen both the buffalo and Sahiwal bull semen. Availability: Items available for loan: UVAS Library [Call number: 0002,T] (1).

2. Immunomodulatory Effect Of Brucella Abortus Vaccine Strain Rb51 In Cross Bred Cows At Private Farms In Lahore

by Shafique, M | Dr. Imtiaz Hussain Khan | Dr. M. Akram Munir | Dr. M. Aleem | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 1999Dissertation note: This project was designed to eradicate dangerous problem (Brucellosis) facing by livestock sector. Various trials of vaccine SRB51 were conducted and serum samples were taken to see the immune status of the animals by using serum tube agglutination test (STAT). For this purpose 100 animals of different age groups were examined and lactating or non lactating animals were randomly selected from different private dairy farms in and around Lahore. The serum samples were collected and subjected to serological tests to detect the presence of antibodies against brucella abortus. The incidence of brucellosis by RBPT was 16% and 14% by Standard tube agglutination test (STAT) in cattle. After screening the animals, we got 30 animals which were brucella abortus free, among these some animals were pregnant. We vaccinated the animal at neck region subcut with Brucella abortus strain RB51 vaccine. After vaccination we found that antibody titer was higher at four weeks and begins to lower at eight weeks and later on, by using STAT. We also observed in this study that no animal was aborted and no premature or weak calf was delievered at parturition. So it can be said that the Brucella abortus strain RB51 vaccine is a safe vaccine and it has been used in a lot number of states of America. The results of this study have revealed an alarming prevalence of brucellosis in cattle at private farms which calls for an emergent response of experts for reappraisal and reassessment of the present brucellosis control situation, especially when the disease is an important zoonosis and potential threat to the human health. It is anticipated that the trials would open new vistas and expose livestock (Dairy) scientists to new horizons of technical know how regarding brucellosis and associated problems. It is further hoped that the results obtained would make tangible contribution towards livestock and dairy industry. Availability: Items available for loan: UVAS Library [Call number: 0589,T] (1).

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