1.
Comparative Efficacy Of Immunomodulators Seleniu, Vitamin E And Herbal Supplements Against IBD Vaccinated Broiler Chickens
by Beenish Zahid | Dr.Muti-ur-Rehman | Prof.dr.Muhammad Younas Rana.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2011Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1248,T] (1).
2.
The Study On Pathogenesis Of Newly Isolated New Castle Disease Virus In Immune And Non Immune Birds
by Muhammad Fahad Aslam | Dr.Muti-ur-Rehman Khan | Dr.Muhammad Younis Rana.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1261,T] (1).
3.
Studies On Molecular Diagnosis And Pathogenesis Of Canine Parvovirus
by Akbar Ali | Dr.Muti-ur-Rehman Khan | Prof.Dr.Muhammad Younas Rana.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2011Dissertation note: Canine parvovirus, caused by a haemagglutinating CPV, is one of the most important acute viral infectious diseases of pups, had been prevalent in the country. In the present study, 50 faecal samples, from clinically suspected cases of parvovirus diseases dogs were collected from pet center of UVAS, Lahore. All the samples collected from the infected dogs were analyzed for complete blood count. There were decrease in the value of certain blood parameters including TEC, TLC, PCV. ESR ,Hb, Plt, MCV,MCH,MCHC. The samples were diluted and centrifuged to collect the supernatant. Being a haemagglutinating (HA) virus, the pre-filtered supernatant from all suspected samples was checked for HA activity using 1% washed chicken erythrocytes. Out of 50 samples, 35 samples were found HA positive. All the HA positive and negative samples were processed for extraction of viral DNA with Genomic DNA purification kit. The net isolated DNA samples were subjected to Polymerase Chain Reaction (PCR) by using specific primers (developed against the variable genomic region of the capsid protein of the DNA) of the CPV. One of the selected primer pair amplified the genomic region present in the CPV-2b. Optimization of PCR was done for the molecular level daignosis of canine parvovirus and this technique was previously not available or standardized in local conditions of Pakistan. With the use of primer pair, best results were found on following optimizing conditions like annealing temperature 55 0C, primers concentration (reverse and forward) 30 pmoles, Magnesium Chloride concentration 2.5mM, DNA (Template) volume 5 µl, Taq DNA polymerase (12.5U) and 200 µM of each dNTPs. The PCR product was analyzed for the banding pattern of 681 bp amplified by the primer pair against the standard DNA ladder (100bp) by using horizental agarose gel electrophoresis. Four of the HA negative samples were amplified by the PCR reaction. and the presence of CPV 2a and CPV 2b in Pakistan as the prevalent pathogenic strains. This study has been a first step for the molecular diagnosis of canine parvovirus in local conditions of Pakistan. It is hoped that this study will pave the way for further advanced studies on this topic.
Availability: Items available for loan: UVAS Library [Call number: 1270,T] (1).
4.
Pathological Changes Induced By Deltamethrin In Japanese Quails (Coturnix Coturnix Japonica) And Its Treatment With Vitamin E and Selenium
by Muhammad Zahid Khan | Dr.Muti-ur-Rehman Khan | Dr.KAmran | Prof.Dr.M.Younus Rana.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2011Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1277,T] (1).
5.
Toxicopathological And Immunosuppressive Effect S In Broilers Induced By Concurrent Exposure To Aflatoxin-Bi And Ochratoxin-A
by Sajid Umar | Prof.Dr.M.Younus Rana | Dr.Aftab | Dr.Muti-ur-Rehman Khan.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2011Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1280,T] (1).
6.
Effects Of Fish Oil On The Histopathology Of Lymphoid Organs Of Broiler Experimentally Infected With Infectious Bursal Disease Virus
by Safdar Fareed Rabbani (2012-va-637) | Prof.Dr.Asim Aslam | Dr.Muti-Ur-Rehman | Prof.Dr.Kamran Ashraf.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Infectious Bursal Disease has become the major poultry disease in Pakistan. The
disease causes immune suppression due to damage to primary lymphoid organs (Bursa of
Fabricius) in affected birds. Supplementation of fish oil enhances the immune status and subside
the inflammatory conditions. So this study was designed to study the effects of fish oil on
histopathology of lymphoid organs and growth performance of broiler birds. For this purpose a
total number of 80 day old Hubbard broiler chicks were randomly divided into four group i.e. A,
B, C and D. Group A and B were challenged with IBD Virus with known (EID50 106/ml) dose
was 0.1 ml intranasal on 21th day of age, Group B and C were treated with 3% fish oil while
group D acted as a negative control. Blood was collected from birds on irregular interval of days
20, 22, 29, 36 and 42 for monitoring of antibody titer against IBD virus and ND vaccine.
For the histopathology the lymphoid organ were collected on day 20, 22, 29, 36 and
42. Feed consumption of group A and D during 1st week of age was significantly higher than
group B and C. The broilers of group A consumed feed without fish oil significantly higher than
broiler of group D while, the broilers of group C consumed feed containing fish oil significantly
higher from the broilers of group D after challenged with IBD. The body weight of birds of
group B and C supplemented with fish oil mixed feed was significantly (p<0.05) higher than
broilers receiving feed without fish oil before challenged, however after challenge the body
weight gain of group C was significantly higher than group B while that of group D was
significantly (p<0.05) higher group A. The feed conversion ratio (FCR) of birds supplemented
with fish oil was significantly different from the broilers receiving feed without fish oil before
challenged.
Summary
48
However after challenged significant difference was observed with highest value of
FCR by group C, followed by group B, group A and group D. Before challenged, group C show
the highest Mean titer value (606.0) for IBDV followed by group B (466.0), C (288.0) and D
(106.5), However, after challenge the highest value of Mean titer for IBDV was observed in
group B with 5006.0, followed by group C, A and D with Mean titer of 4608.0, 4469.0, and
4015.0, respectively on 42nd day of age.
During Histopathological examination, congestion, depletion of lymphocytes, dysplasia
of thymic lobules, thinning of cortex, focal necrosis, disappearance of lymph follicles and
interfollicular edema like lesions were observed in the lymphoid organs of challenged birds.
However, cellular hypertrophy, increased lymphocytes population and presence of lymphoblast
were the prominent changes in lymphoid organs of broilers supplemented with 3% fish oil, while
no pathological lesions were notified in the lymphoid organs of control group. From this study, it
was concluded that Infectious Bursal Disease causes immune suppression and damage immune
organs in broiler, with an associated loss of response to Newcastle disease vaccine.
It was also concluded from the current study that, fish oil had beneficial effect on the
antibody titer against Infectious Bursal disease virus and on growth performance in broilers. The
fish oil also showed therapeutic effect against Infectious Bursal Disease. Availability: Items available for loan: UVAS Library [Call number: 2364-T] (1).
