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Comparison Between Aspiration And Slicing Methods For Retrieval Of Oocytes In Bovine

by Muhammad Husnain (2008-VA-281) | Prof. Dr. Mian Abdul Sattar | Dr. Qaiser Shahzad | Dr. Amjad Riaz | Dr. Muhammad Avais.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Livestock contribution to agriculture stood at 55.9 percent while it contributes 11.8 percent to the national GDP during 2013-14. Buffalo, cattle, sheep and goat population in Pakistan is 34.6, 39.7, 29.1 and 66.6 million numbers during 2013-14. Total milk production from buffalo and cattle as major milk producing animals is 31,252 and 18,027 (000 tons) (Economic Survey of Pakistan 2013-14). Advanced biotechnologies coming from different areas of biological sciences exhibit great promise to enhance the efficiency of livestock production. From these technologies one such biotechnology is the use of in vitro maturation of follicular oocytes and in vitro fertilization for production of livestock embryos in laboratory. Proper oocytes recovery and their selection in the laboratory are of great importance for successful in vitro embryo production. Total one hundred and forty four ovaries (n=144) from cattle (72 ovaries) and buffalo (72 ovaries) were collected and 223 oocytes were retrieved from these ovaries. Average oocytes per ovary were 1.66 + 0.43 oocytes per ovary were obtained via aspiration and 1.89 + 0.00 average oocytes per ovary through slicing method from cattle ovaries. Average 1.55 ± 0.55 oocytes per ovary via aspiration and 1.53 ± 0.20 oocytes per ovary through slicing from buffalo ovaries. Overall grade-A oocytes were 28 (40) percent with aspiration in cattle and 25(36.76) through slicing method. In buffalo overall grade-A oocytes retrieval was obtained in percentage as 20 (44.44) and 26 (52) through aspiration and slicing methods respectively. Grade-B oocytes recovery obtained was in percentage as 23 (33.82) with slicing and 19 (31.67) through aspiration technique from cattle ovaries. Summary 26 Commonly used methods of recovery of oocytes from slaughterhouse animals are aspiration and slicing. Recovery rate of oocytes is different from slaughterhouse ovaries. Aspiration is the best method for retrieval of good quality oocytes from slaughterhouse bovine ovaries because it gave more good quality oocytes in less time than slicing method. In this study, it is found that weight of ovary and no. of follicles/ovary in cattle have strong correlation of 71% existed between weight of ovary and no. of follicles /ovary in buffalo was observed. Correlation between average number of follicles on ovary and weight /ovary was stronger in cattle. The more the number of follicles present on the ovaries and more weight of the ovary, the more will be the recovery of oocytes. In cattle average number of follicles was 10.09 ± 0.30 and when it was checked in buffalo, differed significantly and it was found as 7.16 ± 0.19 on an average per ovary. Likewise weight of buffalo in this study was differed significantly from cattle 4.04 ± 0.10 and 7.62 ± 0.15 respectively. It is suggested that oocytes retrieval should be done in buffalo using aspiration method to retrieve better quality oocytes. It is concluded that aspiration is the suitable method for retrieval of good quality oocytes from slaughterhouse buffalo ovaries because it gave more good quality oocytes in less time than slicing method. But both methods have minor difference between recovery rates but aspiration is more convenient than slicing and it yields more quality oocytes. It is also found that there is very strong correlation existed between average weight of ovary and number of follicles per ovary and the both parameters play a great help for more quality and quantity oocytes. Availability: Items available for loan: UVAS Library [Call number: 2279-T] (1).
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Effect Of Royal Jelly On Post Thaw Semen Quality Parameters Of Beetal Buck

by Muhammad Kaleem (2009-VA-190) | Dr. Abdul Rehman | Prof. Dr. Mian Abdul Sattar | Dr. Muhammad Avais.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Pakistan is an agricultural country. Livestock plays a major role in the agriculture. Among livestock goat population is highest. In Pakistan Beetle is the most important breed of goat and is known as poor man’s cow. This goat is kept for dual purpose for milk and meat production. Artificial insemination is most valuable technique to improve the production and genetic potential of the goat. Cryopreserved semen has many biochemical, structural and functional problems. These detrimental effects produced by cryopreservation compromise the fertility of goat by decreasing post thaw motility, concentration, viability, plasma membrane and DNA integrity. These detrimental effects are due to many reasons among them production of reactive oxygen species is the most important ones. For last many years’ various solutions have been made to overcome the detrimental effect of reactive oxygen species. Various antioxidants are added in all domestic animals and their positive results have been demonstrated by many researchers. RJ is among these antioxidants which improve the sperm parameters. RJ administration in the semen extenders has been shown to improve the post thaw motility, viability, DNA and plasma membrane integrity (PMI) of Beetal buck semen. The study was conducted at Al-Haiwan Sires district Sahiwal, Punjab, Pakistan. For this study 3 regular semen donors Beetal bucks were used for semen collection. Semen was collected twice a week and total 7 collections was taken from each buck. After each collection semen of bucks was pooled to avoid individual buck variations and was divided into 5 equal parts in test tube containing extender with different concentration of royal jelly. These concentrations were . 0%, 0.5%, 1%, 1.5% and 2% RJ. Semen was cryopreserved in LN2.. On post thaw motility, livability, plasma membrane, DNA and acrosome integrity was evaluated. Summary 27 Different royal jelly concentrations were analyzed by repeated measures analysis of variance (ANOVA).Duncan Multiple Range (DMR) test was used to compared the significant differences. Results of different group were expressed as mean ±SEM. The results show that motility%, Plasma Membrane Integrity %, viability %, Normal Apical Ridge % and DNA integrity% of sperms was significantly high at 1% royal jelly concentration as compare to control and other treatment group. Availability: Items available for loan: UVAS Library [Call number: 2722-T] (1).

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