UVAS Library

Your cart is empty.

Your search returned 2 results. Subscribe to this search

Not what you expected? Check for suggestions
Unhighlight Highlight
|
1.
No cover image available
Genetic Characterization Of Pakistani Wild Quails Using Mitochondrial Coi Gene

by Wajiha Shakil (2012-VA-817) | Dr. Ali Raza Awan | Dr. Muhammad Yasir Zahoor | Prof. Dr. Tahir Yaqub.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: A latest taxonomic tool termed as DNA barcoding is being used to genetically characterize animals. DNA barcoding is helpful in determining evolutionary relationship among species. Being a small sized genome and agile enough to show rapid mutation, mtDNA has been used as a pertinent marker of molecular biodiversity. DNA barcode works as an efficient tool in food manufacturing industry, diet investigation, forensics analysis, preventing unlawful trade and felonious poaching. The aim of this study was to develop DNA barcode for genetic characterization of Pakistani wild quail. Pakistani wild quail is important due to its demand for eggs, meat production, experimental purposes and gaming as well. Japanese quail was also included in this study because this quail is excessively produced in Pakistani farms. Japanese quail is present throughout the year and is comparatively bigger in size than wild quail. It has longer lifespan; farmers can easily breed this species in farms. It is suitable in poultry due to better meat yield. COI gene (500bp) was used as a molecular marker for identification at species level. DNA was extracted from blood samples of ten wild quails (Coturnix coturnix and fifteen japanese quails (Coturnix japonica). Reported bird universal primers were used to amplify COI region from the extracted mtDNA samples using PCR. Amplicon were then sequenced by Sanger sequencing method (Sanger et al. 1977). Forward and reverse DNA Sequences were aligned with the reference sequence using nucleotide BLAST on NCBI to observe the dissimilarity among the sequences. Consensus sequences generated were used to construct their phylogenetic tree to see their evolutionary relationship with other bird species. Japanese quail which is thought to be domesticated from Japan, its Pakistani population showed close relation with sequences Summary 90 generated in Japan for this particular species. Pakistani wild quail species showed its closest linkage with C. coturnix. In conclusion, COI barcode proved as an authentic tool for species identification and phylogenetic inference of Pakistani wild and farm grown quails. Wild quail species has been characterized using partial COI gene sequences. This study has provided a specific genetic marker which can differentiate Japanese quail from wild quail at molecular level as most of the time both species are confused with each other. It can be helpful to the farmers and bird fanciers because they can select the birds of their choice correctly. This is the first study reporting DNA barcode of this Pakistani quail species. It would help researchers to study about phylogenetic and taxonomic status and also assist quail fanciers and quail farmers to unaffectedly identify their species of interest in farming. Identification of quail species is also important for conservation of biodiversity as it helps in preservation and identification of endangered species by generating their barcodes from even minimal evidence available. Availability: Items available for loan: UVAS Library [Call number: 2311-T] (1).

Place hold Add to cart
2.
No cover image available
Comparison Of Antifungal Activity Of Human Salivary Histatin Between Diabetic And Nondiabetic Individuals

by Farid-Ul-Haq (2013-VA-555) | Prof. Dr. Tahir Yaqub | Dr. Ali Raza Awan | Dr. Muhammad Tayyab.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Histatins are antimicrobial proteins found in human saliva. These proteins have also been observed to have the ability to aid in wound healing in various organisms. The genes HTN1 and HTN3 have been studied to govern these proteins. Histatin proteins have a vast array of antimicrobial properties. While a fungus, Candida albicans or C. albicans is a part of the human normal gut flora, it is a threat to people who have a compromised immune system. An overgrowth of the fungi belonging to the Candida family leads to candidiasis in humans, and oral candidiasis has been reported to a large extent namely in diabetic patients. The antifungal activity of histatin proteins laid the basis of the current research work. In this study, the antifungal activity of saliva from a total of 64 healthy and diabetic human samples against Candida albicans has been evaluated. The samples of both healthy and diabetic human samples belong from different age ranges: 15-25, 25-35, 35-45 and 45-55 years in order to change in antifungal activity with respect to age of an individual. Antifungal activity was observed through both agar well and agar disk diffusion methods, with agar disk diffusion methods showing positive results. According to the outcomes of this study at least 120μL of healthy saliva sample is required to create a zone of inhibition. Saliva from diabetic individuals showed no antifungal results. This occurrence led to the next part of this study involving amplification of HTN3 gene. The nucleotide sequences of both healthy and diabetic individuals were compared together and showed that the absence of antifungal activity in diabetic individuals might have reasons other than a genetic one, according to this study. The results observed from the present study indicate that healthy human saliva possesses antifungal activity against Candida albicans. In accordance Summary 39 to these results, the naturally occurring antimicrobial activity of histatin proteins present in human saliva can have immense use in the field of medicine. Availability: Items available for loan: UVAS Library [Call number: 2341-T] (1).

Place hold Add to cart

Implemented and Maintained by UVAS Library.
For any Suggestions/Query Contact to library or Email:rehana.kousar@uvas.edu.pk Phone:+91 99239068
Website/OPAC best viewed in Mozilla Browser in 1366X768 Resolution.