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1. Immuo-Pathological Response Of Pigeons To Challenge Infection Of Newcastle Disease Virus (Ndv)

by Yasir Amin | Dr. Asim Aslam | Prof. Dr. Muhammad Younus Rana.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2011Dissertation note: This study trial was designed to evaluate and compare the efficacy of two locally available live Newcastle disease vaccines (Medivac ND LaSota and VRI, Mukteshwar ND vaccine), also to compare two routes (Oral and Ocular) of vaccine administration in term of antibody titre and assessment of protection against field (chicken) isolated virulent Newcastle virus challenge (NDV) in pigeons. Study of clinical signs, gross and histopathological lesion in different organ of non-vaccinated and challenged birds was also the part of our present study. For this purpose one hundred and twenty pigeons were purchased from the local market and screened for Newcastle disease antibodies using Hemagglutination inhibition test. Healthy pigeons were randomly divided into six groups i.e. A, B, C, D, E and F, comprising 20 birds each. Group E and F were kept as positive and negative Control respectively. Group A and C were vaccinated with Medivac ND LaSota vaccine at day 7th and 21st of experiment through oral and ocular route. Similarly Group B and D were immunized with VRI (Mukteshwar) ND vaccine through oral and ocular route respectively. At 28th day of experiment all the groups except group F were challenged with velogenic field isolate of NDV at a dose rate of 0.1 ml through ocular route. Serum samples were collected at day 14, 21, 28, 35 and 42 of experiment for the determination of antibody titre. Post-infection clinical signs in control positive group were i.e. anorexia, dullness, depression, decreased feed intake, discharge from mouth, greenish diarrhea, nervous manifestations, leg and wing paralysis. Gross lesions on different organs were hemorrhages in trachea, proventriculus, spleenomegaly and greenish intestinal contents. Medivac ND LaSota vaccine produced higher immune response in term of antibody titre as compared to VRI (Mukteshwar) ND vaccine. It was also observed that ocular route irrespective to vaccine type produced significantly (P<0.05) higher immune response than oral route. Vaccine strains used in this study efficiently induced immune response through ocular route, suggesting that implementation of this vaccination programs in future may prevent ND outbreaks in pigeons, especially in racing pigeons, and may prevent NDV spread to other avian species, mainly poultry. Availability: Items available for loan: UVAS Library [Call number: 1228,T] (1).

2. Toxicopathological Alterations And Tissue Residue Of Colistinsulphate (Polymixin E) In Broilers

by Muhammad Aslam (2012-VA-809) | Dr. Muhammad Raza Hameed | Prof. Dr. Muhammad Younus | Dr. Syed Ehtisham ul Haque.

Material type: book Book; Literary form: not fiction Publisher: 2014Dissertation note: Poultry industry is the 2nd largest industry in Pakistan playing important role to meet daily protein requirement with an investment of more than 200 billion rupees. It is contributing 6.1% and 10.8% share in total contribution of agriculture and livestock the national GDP, respectively. Total poultry meat production in the last year was 987 thousand tones (Economic survey of Pakistan 2013-2014). Because of high density and lack of biosecurity measures, outbreaks of infectious disease are more common and often occurs. Different antibiotics are used rational and irrational to treat and control these outbreaks. Colistin is among the most widely used antibiotic in intensive poultry farming, it is used to combat gram negative bacterial infections particularly for collibacillosis and gastrointestinal tract infections (Hussain and Khalil 2013). Colistin belongs to the polymixins group of antibiotics. It is also known as Polymixin E. Polymixins are structurally related substances consisting of a cyclic peptide with a hydrophobic tail, mainly produced from gram positive nitrogen fixing bacteria Bacillus polymyxa. Polymixins are classified into six subtypes namely polymixin A to F and among these polymixin B and E are commonly used for the treatment of gram negative infections in animal and human beings. Colistin is mixture of two subtype’s colistin A (polymixin E1) and colistin B (polymixin E2). Commercially, it is available in two forms colistin sulfate and colistimethate sodium but colistin sulfate is more stable cationic water soluble salt (Landman et al. 2000) and is easily available in markets. colistin sulphate has lipophilic and lipophobic(hydrophilic) properties, that makes it easier to interact on bacterial cell membrane by displacing counter ions in lipopolysaccharides, influencing Mg efflux that’s why it is effective against gram negative bacteria (Poole and Sheffield 2013). Polymixins also presents antipyretic activity by binding endotoxins, especially lipid A, (the active compound of lipopolysaccharide). Owing to this property it is extensively used to treat gram negative infections in livestock and poultry industries (Hanasawa et al. 1990). The use of colistin was restricted in the past due to its toxic properties mainly in nephrotoxicity. There are reports available in the literature indicating that the parental use of colistin leads to the alterations of biochemical parameters in chicken i.e. increase in glutamic oxaloacetic transaminase, catalase activity, creatinine while, adversely affect total plasma proteins (Ibrahin et al. 2011). In ostriches it leads to the development of neurotoxic signs along with histologically odema formation in pericardium, intestinal serosa and heart was observed (Landman et al. 2000). Toxic effects of colistin were also reported in humans including nephrotoxicity, increased serum creatinine during the treatment (Falagas et al. 2005). As bioavailability for oral route is minimal and it is used parentally to treat systemic infections. Parenteral route may cause toxopathological effects in birds. Other effects includes change in aspartate aminotransferase and alanine aminotransferase which indicate liver damage. Red blood cells, white blood cells, packed cell volume and hemoglobin estimation were also decreased (Saleemi et al. 2013). The incidence of nephrotoxicity is severe but less common in oral administration as polymixins absorption through gastrointestinal tract is very slow and limited, and cannot be detected in plasma concentrations at ordinary doses (Falagas and Kasiakou 2006). Instead of all these toxic effects, now a days it is frequently used for the treatment against gram negative bacterial infections (Vaara 2010). The emergence of bacterial resistance has become an important public health hazard throughout the world). Due to irrational use of antimicrobial drugs against different diseases (Naqvi et al.2013. Colistin is one of the most widely used antibiotic in poultry industry against diseases like collibacillosis, salmonellosis and clostridial infections through drinking water, feed additives and parenteral route in developing countries like Pakistan (Tanweer et al.2013). Parenteral use of colistin sulphate may leads to the deposition of residues in different tissues. The presence of its residues in edible parts may lead to the toxicity as well as development of antibacterial resistance in human. Keeping in view the potential toxic effects and other hazards relating to the extensive use of colistin in poultry birds, present study has been designed to investigate dose dependent patho-morphological alterations and tissue residue in broiler chickens. Objective 1. To study the toxicopathological effects of parenteral administration of colistin in broilers. 2.Investigation of the tissue deposition of colistin as residues and its withdrawal period. Availability: Items available for loan: UVAS Library [Call number: 2221-T] (1).

3. Immunomodulatory Effect Of Hing (Ferula Foetida) Along With Infectious Bursal Disease (Ibd) Vaccine In Broiler

by Muhammad Javed Raza | Prof. Dr. Muhammad Younus.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: A number of feed additives including antibiotics have been extensively used in poultry feeds. However, the use of antibiotics has been restricted due to the drug resistance and the issue of residues in meat. Now a day, the use of medicinal plants is being popular as an alternate remedy. Asafoetida is a natural feed additive and antimicrobial, immune stimulator, antiviral, antifungal, anti-parasitic, antithrombotic, antioxidant, anti-cancerous, and vasodilator activities. It is being used for the control of some viral problems. Some previous studies also indicated that it has beneficial effects on the immune system. Therefore, the present study was designed to estimate the immunomodulatory effects of F. foetida with IBD vaccine in enhancing the immune system and ultimately increase the production of poultry products. A total of n=90 day old broiler chicks were purchased and kept under the optimum conditions at CVAS Jhang. Birds were divided into three groups A, B, C and group B and C were further divided into three subgroups (B1, B2, B3 and C1, C2, C3). Group A was treated as positive control group and was administered with commercially available IBD and ND vaccine and routine diet while group B was administered with asafoetida at the rate of 2%, 4%, and 6% along with vaccine to see the impact of different levels of asafoetida on the immune system and to see the effects (if any) of various doses of asafoetida. Group C was only administered with asafoetida in feed at the same rate as to group B. At the end of study birds were slaughtered to observe the effects of asafoetida administration. Positive effect of asafoetida has been reported by many studies. F. foetida is a medicinal herb used for the prevention and treatment of many diseases, because of having antiviral, antibacterial and antifungal activities. Also act as a good growth promoting agent and have beneficial effects on the immune system. So, it was hypothesized that the present Summary 39 study was helpful to determine the immunomodulatory effect of asafoetida for commercially available IBD vaccine and could reduce the cost of vaccination for commercial poultry farmers. Availability: Items available for loan: UVAS Library [Call number: 2562-T] (1).

4. Immunomodulatory Effects Of Feeding Allium Sativum Against Infectious Bursal Disease (IBD) Vaccinated Broiler Birds On IBD Vaccine.

by Uzma Riaz (2008-VA-286) | Prof. Dr. Muhammad Younus | Dr. Muhammad Asif Idrees | Dr. Iahtasham Khan.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: A number of feed additives including antibiotics have been extensively used in poultry diets for the purpose of weight gain to improve feed efficiency and growth rate. However, use of antibiotics has restricted due to the bacterial resistance and the issue of residues which make the chicken meat harmful for human consumption. So the medicinal plants are gaining interest as alternative feed strategies now a day because of their low cost, easy availability and presence of no residues. Garlic was used for the medicinal purposes and as a health supplement by the ancient Egyptians. It is a natural feed additive and is antimicrobial, immune stimulator, antiviral, antifungal, anti-parasitic, antithrombotic, antioxidant, anti-cancerous, and vasodilator activities. Previous studies indicate that it has beneficial effects on the immune system and is a best immune stimulator then the other herbal plants and medicines. Therefore, the present study was designed to estimate the immunomodulatory effect of garlic to commercially available IBD vaccine in enhancing the immune system. Total N=99 day old broiler chicks were purchased and kept in the experimental shed of CVAS Jhang. Birds were divided into three groups A, B, C and group B and C were further divided into three subgroups (B1, B2, B3 and C1, C2, C3). Group A was treated as control group and was administered with commercially available IBD and ND vaccine and routine diet while group B was administered with garlic at the rate of 4%, 5%, and 7% along with vaccine to see the impact of different levels of garlic (Allium sativum) on the immune system and to see the toxic effect (if any) of high dose of garlic. Group C was only administered with garlic in fee Summary 44 at the same rate as to group B. At the end of study birds were slaughtered to check the effects of garlic administration. Positive effect of garlic has been reported by many studies. Garlic is a medicinal herb used for the prevention and treatment of many diseases, because of having antiviral, antibacterial and antifungal activities. Also act as a good growth promoting agent and have beneficial effects on the immune system. Results of the study indicate that administration of garlic powder in different doses alone and combined with commercial IBD vaccine have good effects on the growth, blood parameters and the immune system of the broiler birds. Availability: Items available for loan: UVAS Library [Call number: 2561-T] (1).

5. Effects Of Aqueous Extract Of Neem (Azadirachta Indica) Leaves Against Colibacillosis In Broiler

by Muhammad Azfar (2014-VA-1111) | Dr. Asif Idrees | Prof. Dr. Muhammad Younus | Dr. Iahtasham Khan.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: In Pakistan the poultry sector has developed rapidly in the last two decades. The poultry production as practiced today is specialized one and concentrating more on the use of high performance birds. The major factors for successful poultry production are high genetic potential, balanced nutrition and health maintenance (Nayaka et al. 2012). Utilization of immunostimulant is one solution to improve the immunity of animals and to decrease their susceptibility to infectious diseases. Immunostimulation comprises a prophylactic and therapeutic concept aimed at stimulation of the non-specific and specific immune response (Hyde and Patnode, 2001). Most of the commercial poultry growers use antibiotics as growth promoters and to reduce the chance of occurrence of infectious diseases, which usually result in higher costs of production and ultimately lower net returns. There are great concerns about the use of antibiotics as therapeutic immunomodulators and growth promoters as it has given rise to antibiotic resistant bacteria. In this modern world of science, the researchers are once again intending towards exploring the potential benefits of the conventional medicines. Neem (A. indica) is an herbal plant which is known to exhibit various beneficial pharmacological properties including immunomodulatory effect in broilers. Poultry sector is facing some problems one of which is bacterial disease which cause economic loss. Farmers use different antibiotics as growth promoter and to reduce the incidence of different diseases on poultry which increases cost of production and lowers the net returns. In addition, use of antibiotics giving rise to other problems like increasing drug resistant against these antibiotics especially Gram –ve bacteria (Salmonella and E. coli). It’s necessary that antibiotic residues in meat would not adversely affect human health. Specifically, it has been recommended that the penicillins, tetracyclines, tylosin, and Summary 54 sulfonamides as growth promoters should not be use any more. So scientists are giving attention to the use of indigenous medicine instead of antibiotics for better rearing of poultry. Pakistan is blessed with different medicinal plants one of which is Neem (Azadirachta indica). For thousands of years the medicinal properties of Neem have been recognized. Each part of the Neem tree has some medicinal property. Biological Activity of Neem is due presence of different chemicals like nimbolide, nimbin, nimbindin, quercetin among others. Biological activities include Anti-inflammatory; Antiarthritic; Antipyretic; Hypoglycemic; Antigastric ulcer; Spermicidal; Antifungal; Antibacterial; Diuretic; Antimalarial; Antitumour; Immunomodulatory A. indica grow fast and is evergreen tree which has medicinal and nutritive value for broilers. Broilers which are given Neem leaves extract in water have shown improved FCR and weight gain. This medicinal Plants compete with synthetic drugs as it strengthens the immune system by activate Cell mediated immunity and have no residues in meat. Neem extracts also improve performance and hematological parameters However, I did the research work in short-term basis (only 42 days) and modern equipments were also not available. Therefore, an extensive research and development work should be undertaken on Neem and its products to make the findings more accurate and effective for their better economic and therapeutic utilization. Availability: Items available for loan: UVAS Library [Call number: 2613-T] (1).



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