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Determination Of The Hepatitis C Virus Genotyping Prevailing In The Hepatitis Suspected Patients In District Mardan,

by Suliman Qadir Afridi | Prof. Dr. Tahir Yaqub | Dr. Fariha Akhtar | Dr. Yasin Tipu.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1469,T] (1).

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2.
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Detection Of Amantadine Resistant Variants Among Avian Influenza Viruses Subtype H9n2 Isolated In Pakistan

by Sabir Subhan (2009-VA-32) | Prof. Dr. Tahir Yaqub | Dr. Muhammad Zubair Shabbir | Dr. Yasin Tipu.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Avian influenza A virus subtype H9N2 is prevalent in poultry industry of Pakistan. Amantadine is an antiviral drug which is being used as prophylactic measure to control this disease despite the occurrence of resistance against this drug. There is need to monitor the resistance of amantadine in Flu viruses. In this study, we collected 100 samples of broilers birds showing mild to severe respiratory signs. Samples were collected from different locations of Punjab, Pakistan. After initial identification via Hemagglutination test, the molecular identification and confirmation of subtype H9N2 was done by multiplex PCR. To rule out the co-infection of NDV, PCR of NDV was also done. The samples which were pure H9N2 were further processed for the screening of amantadine susceptibility. To do this, titration of viruses was done on MDCK cells in the presence and absence of amantadine at the concentration of 2 ug /ml. The results of TCID5O were compared in the presence and absence of amantadine for each isolate and isolates showing difference of 2 log 10 TCID50/0.1 ml were declared resistant to amantadine as described by Masuda et al. 2000. The results of this study revealed that the viruses circulating in the poultry industry if Pakistan are resistant to this drug as we found that out 10 isolates 4 were resistant to this drug. So, there is need to monitor the usage of this drug in poultry as human cases of H9N2 viruses have been reported and virus was of avian origin. Monitoring is necessary because amantadine is recommended in flu pandemics and this virus possesses the pandemic potential and can cross the species barrier. Availability: Items available for loan: UVAS Library [Call number: 2457-T] (1).

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3.
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Pathological Investigations Of Different Isolates Of H9n2 Prevalent In Broiler Chicken

by M. Furqan Shahid (2014-VA-322) | Dr. Yasin Tipu | Prof. Dr. Asim Aslam | Prof. Dr. Tahir Yaqub.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: In recent years, H9N2 virus has attained a great importance as its infection has reached panzootic proportions. AIV H9 has different antigenic variants that has made it problematic to diagnose and thus to understand the pathogenicity of this virus is also very difficult. Detection of AI H9 antibodies can be used as a complementary method for sero-epidemiological studies as an indicator of AI H9 infection. The haemagglutination inhibition (HI) assay is used routinely for subtyping and detecting an increase of antibodies to AI viruses. Surveillance and early diagnosis of AI virus is essential for poultry. It demands rapid, sensitive and inexpensive diagnostic tests. Thus, it is important to identify different antigenic variants of H9. In this study a total of seven H9 virus samples were isolated out of total 100 collected sample from field outbreaks. These isolates were confirmed by molecular methods like PCR. Then four isolates from these seven isolates were used to infect the experimental broiler chicken. Clinical signs were recorded after the inoculation of H9N2 virus to the broiler. The results of this study showed that clinical signs were more sever upto 5 DPI. The severity of signs was proved by observing the gross pathology and histopathology of organs (Lung, Kidney, Trachea and Liver) of infected birds which were collected on 5 and 9 DPI. Serum of infected birds was also collected on 7 and 14 DPI to analyze the antibody level of infected birds against experimentally used isolate of H9N2. Then cross reactivity of different isolates of H9N2 was also checked against pannel of hyperimmune sera raised against different isolates of H9N2 and their results showed different antibody level against different isolates of H9N2. The sero-biochemical study of serum of infected birds revealed that H9N2 virus has pathogenic potential on kidney and liver. Availability: Items available for loan: UVAS Library [Call number: 2459-T] (1).

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4.
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Studies on Pathogenesis and Molecular Diagnosis of Infectious Bursal Disease Virus In Broiler Chicken

by Beenish Zahid (2003-VA-134) | Prof. Dr. Asim Aslam | Dr. Yasin Tipu | Prof. Dr. Tahir Yaqub.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Blank CD. Availability: Items available for loan: UVAS Library [Call number: 2510-T] (1).

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5.
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PREVALENCE OF MAJOR BACTERIAL AND VIRAL POULTRY DISEASES IN LAHORE DIVISION OF PAKISTAN

by Wasiq mehmood (2009-VA-420) | Prof. Dr. Tahir Yaqub | Dr. Aamir Ghafoor | Dr. Yasin Tipu.

Material type: book Book; Literary form: not fiction Publisher: 2017Dissertation note: Poultry is a huge industry as an emerging agribusiness in Pakistan that contributes a lot in GDP of country but infectious diseases contribute as a major obstacle in profitable production of poultry. There is need to study the most current scenario of major diseases together with all the parameters involved. This can help in making effective preventive measures to minimize the losses. In this study we investigated 1008 cases of poultry diseases received at GP Lab, Lahore. Sick and dead birds were received from different locations of Lahore division during November, 2015 to November, 2016. Whole year was divided in to five seasons as winter (15-Nov to 15-Feb), spring (16-Feb to 14-April), hot summer (15-April to June), hot humid summer (July to 15-Sept) and autumn (16-Sept to 14-Nov). Disease was diagnosed on the basis of flock’s history, postmortem findings, isolation and identification of pathogens using various techniques of bacteriology, virology and molecular biology along with different serological techniques. The result of this study revealed that both bacterial and viral health risks are prevailing in Lahore division of Punjab however bacterial problems are greater in number in comparison of viral infections. Prevalence of E. coli infection (Colibacillosis) was greater than any other disease which could be due to poor disinfection and cleaning of control sheds along with poor management of flocks. Avian influenza and ND shared more than 90% of viral problems throughout the year. Mean prevalence of IBD was found to be 1.46% in recent year whereas CAV and adenoviral infection remained up to negligible extent. Very few cases of CRD and necrotic enteritis were reported. Prevalence of diseases has a strong correlation with seasons with incidence highest in winter and hot summer which could be due to challenging management of flocks because of severe climate conditions. Incidence in spring was found out to be 19.46. Hot humid summer and autumn were found to be least harmful seasons with prevalence of 11.23% Summary 66 and 7.11% respectively. In order to cope up with health issues, up to date studies on prevailing poultry diseases needs to be done in upcoming years as well. Availability: Items available for loan: UVAS Library [Call number: 2822-T] (1).

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