1.
Impact Of Digestible Lysine, Methionine + Cysteine Ratios On Growth Performance, Ileal Digestibility Of Protein And Gut Health In Broilers Fed Different Protein Sources
by Ch. Ahsan Akram (2009-VA-397) | Prof. Dr. Talat Naseer Pasha | Dr. Shafqat Nawaz Qaisrani | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Poultry production is a vibrant segment of agriculture in Pakistan. Genetic selection and nutritional improvements resulted in accelerated growth rate and better FCR in modern broilers. This improved broiler performance requires greater amount of digestible nutrients. Since feeding cost accounts about 70% of total production cost and protein is the most expensive component of poultry diets. Future poultry diets, therefore, will be formulated by using cheaper and poorly digestible protein sources such as CM and SFM due to price volatility of highly digestible but expensive sources like SBM. The undigested protein in hind gut is subjected to microbial fermentation, results in formation of nitrogenous products that are not only harmful to birds, but also excreted into litter bedding and generate ammonia, nitrous acid and nitrous oxide, the latter one is a powerful greenhouse gas with an effect about 300 times compared with carbon dioxide. The potential solution to this problem is supplementation of synthetic amino acids in low nutrient density (CP) diets formulated by using cheaper and poorly digestible protein sources. Therefor a study was designed with the objectives to determine the impact of dLys, M + C ratios on growth performance, apparent Ileal digestibility of protein and gut health in broilers fed poorly digestible protein sources (CM and SFM). An experiment with 432 mixed sex (Ross 308) 1-d-old broilers was conducted to test the hypothesis that supplementation of Meth may improves growth performance, apparent Ileal digestibility of protein and gut health in broilers fed poorly digestible protein sources (CM and SFM). The interaction effects of the protein source (CM vs. SFM), CP levels (high vs. low) and dM+C/d Lys (recommended vs. supplemented) were tested in a factorial arrangement of 8 (2 × 2 × 2) dietary treatments. The supplementation of Meth positively influenced FI (P = 0.042), BWG (P = 0.003) and FCR (P <0.001). The broilers fed Meth supplemented diets had, on an average, 10% greater apparent Ileal digestibility of protein, 34% longer villi, 24.5% shorter crypts and 60% greater VCR compared with those fed diets with recommended levels of Meth. The levels of CP, similarly, influenced (P <0.001) the FI, BWG and FCR. The broilers fed high CP diets had 9% improved apparent Ileal digestibility of protein compared with those fed low CP diets. In conclusion, supplementation of Meth improves the broiler growth performance, apparent Ileal digestibility of protein and gut health fed poorly digestible protein sources (CM and SFM). The negative effects of poorly digestible protein sources thus may be partially ameliorated by supplementation of Meth.
CONCLUSIONS
In conclusion, the negative effects of poorly digestible protein sources thus may be ameliorated by supplementation of Meth. Supplementation of Meth above the recommended levels improves the growth performance, Ileal digestibility of protein and gut health in broilers fed poorly digestible protein sources (CM and SFM).
SUGGESTION AND RECOMMENDATION
Further research is needed to evaluate the different strategies to improve the digestibility of poorly digestible protein sources including guar meal. Threonine also has an important role in gut health so there is a need to study the effects of supplementation of threonine above the recommended levels in broilers fed poorly digestible protein sources. Feed form has pronounced effects on gut development, digestibility of nutrients and subsequent growth performance of birds. There is a dire need to evaluate the other cheaper protein sources, supplemented with synthetic amino acids and to find out a possible interaction between feed form and poorly digestible protein sources that may replace SBM in broilers.
Availability: Items available for loan: UVAS Library [Call number: 2567-T] (1).
2.
Effect Of Methionine And Mannan Oligosaccharides Supplementation On Growth Performance, Carcass Characteristics, Immunity And Selected Blood Metabolites In Broiler Chicken Under Conditions Of High Ambient Temperature And Humidity
by Asif Mahmood (2009-VA-379) | Dr. Muhammad Afzal Rashid | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Poultry sector is one of the most organized and vibrant segment of agriculture sector in Pakistan and is claimed to be among the largest industries of Pakistan with over more than 200 billion rupees investment and offering employment directly or indirectly to 1.5 million people. Broilers exposed to high environmental temperature and humidity exhibit several physiological and performance related setbacks. Heat stress cause high mortality, poor FCR, reduced feed consumption, body weight gain, and visceral development. Heat stress has been linked to a decrease in intestinal immune activity and to impaired intestinal morphology following pathogenic bacteria invasion of the body through the intestinal epithelium. Nutritional manipulations with its low cost are preferred to alleviate the negative effects of heat stress on performance. Nutritional strategies such as the addition of prebiotics, some amino acids e.g. methionine are reported to be beneficial in alleviating heat stress. As a nutritional intervention, synthetic methionine added to the diet has been explored as method of ameliorating the effects of ROS associated with high environmental temperature. Keeping above in view, the present study has been planned to evaluate the effect of supplementing methionine and MOS on the growth performance, carcass characteristics and immune status against ND under field conditions. In this experiment a total of n=200 day old chicks were procured from a commercial hatchery and randomly assigned to dietary treatments with n=40 chicks per treatment. Each treatment was further divided into four replicates with n=10 birds/replicate. Dietary treatments were : 1) negative control (NC) without any supplementation, 2) positive control (PC) containing antibiotic, 3) MOS supplemented (MOS) containing 800 and 400 g/ton of MOS in the starter and grower, respectively, 4) High Methionine supplemented treatment (HM) containing Methionine 1% of the diet in both starter and grower feeds, 5) Methionine and MOS supplemented (HM+MOS) treatment containing high methionine
SUMMARY
42
(1% in starter and grower) along with 800 and 400 g/ton of MOS in the starter and grower, respectively. The temperature and humidity were recorded daily at 8:00, 14:00 and 18:00 h and means were calculated to determine the daily changes in temperature humidity index. Feed intake, body weight and FCR were recorded at the end of each week. At the end of experiment n=3 birds per replicate were selected, weighed and slaughtered. At the time of slaughtering blood samples were collected in evacuated tubes. Serum was harvested after centrifugation, dived into aliquots and stored at –20 oC until further analysis. Serum samples were used to determine antibody titer against ND. At the time of slaughtering organ weighed and jejunal sample were collected to determine the gut morphology. The data collected were analyzed using analysis of variance (ANOVA) technique in a Completely Randomized Design (CRD). Results were declared statistically significant at P < 0.05 and compared using Tukey, s multiple comparison test. The statistical analysis were carried out using SAS 9.1 (SAS Institute, 2006). Live BW was higher (P<0.05) in HM+MOS compared to NC and HM. Feed intake was higher (P<0.05) in HM+MOS and PC than those in NC. However, FCR was lowest in HM+MOS compared to NC and PC birds. Relative organ weights were similar (P>0.05) among treatments; however, bursa of fabricius was heavier in MOS compared to the NC and HM+MOS. Villus height and villus to crypt ratio was highest (P<0.05) in HM, than the NC treatment birds CONCLUSIONS In conclusion, supplementation of methionine at 1% of diet and MOS improved the weight gain, feed intake and feed conversion ratio in birds reared under conditions of high temperature and humidity. Gut morphology of the bird also improved by supplementation of Methionine and MOS in the broiler diet.
SUMMARY
43
SUGGESTION AND RECOMMENDATION
Further research is needed to re-determine the recommended levels of methionine in the diet when bird are reared under the conditions of high temperature and humidity. Availability: Items available for loan: UVAS Library [Call number: 2606-T] (1).
3.
Protease Supplementation Influences Growth Performance, Gut Health And Ileal Digestibility Of Protein In Broilers Fed Low Protein Diets
by Shahzad Ahmad (2008-VA-240) | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Proteases are protein-digesting enzymes that are used to break down the proteins of various plant materials and proteinaceous anti-nutrients in plant protein feedstuffs. The use of exogenous proteases can help in the reduction of the protein contents of a given feed by improving the digestibility and availability of the protein thereby reducing the cost of feed contributed by the protein feedstuff. It will also help to reduce the nitrogen excretion by the poultry and will contribute to mitigate the climate change.
In the present study the protease from a bacillus lachniformis spp was supplemented to evaluate the effect of enzyme on protein digestibility, gut health and birds performance. For this three experimental diets with 21, 19 and 17 % CP (HP, MP and LP) with six treatments with and without exogenous protease were used in this trial to evaluate the effect of protease. Each treatment was replicated to 6 pens and 10 birds were allocated to each pen. Parameters studied regarding birds performance were feed intake, weight gain and FCR. All the growth performance parameters were recorded on weekly basis while the Ileal digestibility and gut health parameters were recorded at the 35th day of the trial. Data was analysed through completely randomized design (CRD) under factorial arrangement using ANOVA technique (Steel et al. 1997). Means were separated through Duncan’s Multiple Range test (Duncon’s, 1955) with the help of SAS 9.1.
The results of feed intake, weight gain and feed intake: gain on weekly basis showed inconsistent results, however the overall performance of the birds fed low protein diets supplemented with protease showed significant results. The gut health parameters showed the significant increase in villus height. The apparent Ileal digestibility of protein was significantly increased in the MP supplemented with protease enzyme.
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CONCLUSION
Based upon the findings of this study it can be concluded that;
Supplementation of protease improves growth performance of the broilers however the results on weekly basis were inconsistent but overall growth performance data shows significant results.
Protease supplementation enhances the CP digestibility in 19 % diets compared to 21 % and 17 % CP diets.
The supplementation of protease enzyme improves the villus height and crypt depth: villus height in broilers.
SUGGESTIONS AND RECOMMENDATIONS
Future researchers can further evaluate the subsequent effects of the protease on growth performance and immune response in broilers.
In future the research can be conducted to determine the effect of protease on energy digestibility and immune response in broilers. Availability: Items available for loan: UVAS Library [Call number: 2601-T] (1).
4.
Effect Of Feed Form And Particle Size Of Corn On Growth Performance, Protein Digestibility And Gut Health Of Broiler
by Shahzada Muhammad Zeshan Haider (2014-VA-919) | Prof. Dr. Talat Naseer Pasha | Prof. Dr. Saima | Prof. Dr. Athar Mahmud.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: The presented study was conducted at control house of Department of Poultry Production, C-block, UVAS Ravi Campus. In total 480, day-old Hubbard Classic broiler chicks were procured from local hatchery, weighed at 1st day and randomly distributed to eight treatment groups in such a way that there were six replicates in each group and each replicate had 10 chicks. Eight isocaloric and isonitrogenous experimental diets were formulated. Half birds were offered crumble form of feed in whole life while half birds were offered crumble form in starter phase and pellet form in finisher phase. Four levels (<1, 1-1.5, 1.5-2 and 2-2.5mm) of corn particle size were used in each form of feed.Body weight and feed consumptionwere recorded weekly in order to calculate feed conversion ratio.Data collected were analyzed by ANOVA using factorial arrangement under CRD. Experimental means were compared using DMR test.
Birds fed diet having higher particle size (2-2.5mm) of corn had lower feed consumption, greater body weight gain and improved FCR compared to those fed smaller particle size during starter, finisher and overall phase. Further, birds fed pelleted feed had lower feed consumption, greater weight gain and improved FCR than those fed crumble form of feed.Further, different particle size of corn resulted in non-significant effect on villus height and crypt depth. Feed form also had non-significant influence on villus height and crypt depth of duodenum.Birds fed diet having higher particle size (2-2.5mm) of corn had higher apparent ileal digestibilityof protein than those fed smaller particle size. Feed form had non-significant effect on apparent ileal digestibilityof protein.
It can be concluded from the results that birds fed pelleted diet containing coarse particle size had improved growth performance and protein digestibility without adverse effect on gut health of broiler.
Availability: Items available for loan: UVAS Library [Call number: 2879-T] (1).
