Your search returned 5 results. Subscribe to this search

Not what you expected? Check for suggestions
1. Passive Immunization Against Canine Distermper Virus In Dogs

by Ali Ahmed Malik | Prof. Dr. Masood Babbani | Prof. Dr. Khushi Muhammad | Faculty of Veterinary Sciences.

Material type: book Book; Format: print Publisher: 2005Dissertation note: Canine distemper is an important, highly contagious disease of dogs, caused by morbillivirus of family paramyxoviridae. The disease occurs worldwide in variety of hosts. In the present study, data relative to breed, sex and age susceptibility in clinically suspected cases of canine distemper was collected and analyzed. The disease is mostly seen in young nonvaccinated dogs of 4 to 6 months of age when maternal anti-CDV antibodies are decreased. Immune serum was raised in experimental dogs with commercially available measles live virus vaccine. The level of antibodies in the immune serum was determined by agar gel precipitation test (AGPT) and an ELISA based assay. Immune serum containing 128 AGPT units of anti-CDV antibodies was effective to control the disease in infected dogs after natural exposure to canine distemper virus. Finally the effective time for passive immunization against canine distemper was determined in experimental dogs. It was noted that immune serum offered protection to canine distemper immediately after infection, during the incubation period of the disease , 48 hours after infection and early phase of the disease(at the appearance of clinical signs). Passive immunization is not rewarding in the terminal phase of the disease (when infected dogs show nervous signs of the disease).Thus it is very useful for the prevention of disease in dogs kept with infected dogs in kennels and pet shops. Availability: Items available for loan: UVAS Library [Call number: 0882,T] (1).

2. Isolation And Molecular Characterization Of Antimicrobial Resistant E-Coli Isolation From Retail Meats

by Ali Ahmed | Prof.Dr.Khushi Muhammad | Dr.Mueen Aslam | Prof.Dr.Masood.

Material type: book Book; Format: print ; Nature of contents: biography; Literary form: Publisher: 2010Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1252,T] (1).

3. Impact Analysis Of Quality Control Practices In Selected Microbiology Based Veterinary Diagnostic Labs Operational In District Lahore

by Faiza Marrium (2009-VA-239) | Prof. Dr. Masood Rabbani | Dr. Ali Ahmed Sheikh | Dr. Yasin Tipu.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Veterinary diagnostic labs are the powerful ally for the diagnosis, prevention and monitoring of animal diseases in any country. Labs are ordered for test for many reasons. Errors could be present in these tests. So to avoid detrimental effects of these errors a quality control system is required. This system is maintained by following the standards given by recognized international organizations like ISO, OIE, WHO, FAO and CDC etc. These authorities make the standards which should be followed by the VDLs to improve their quality of tests and management to give precise and accurate results which will help them in being well reputed lab which give internationally accepted quality of results. In this study 4 private and 4 public sectors Veterinary diagnostic Labs was selected and it was assured to labs that their information will remain confidential as data was used only for the research purposes. Labs were identified by the codes given to them for study purpose. Information required for this study was gained through a questionnaire. Information regarding identity of lab, contact numbers, location and type of testing/diagnosis was gathered. Information about the parameters of quality control like building design, power backup, sections of lab, operating equipments, development of log books, availability of certificate of analysis for chemicals, availability of material safety data sheet was gathered. Quality assurance issues were also addressed by gathering information about internal quality assurance program, proficiency testing etc. Then this data was analyzed by using SPSS to interpret the results. The data was analyzed statistically through frequency distribution by using Statistical Package for Social Sciences (SPSS) version 18.0 for development of Graphs and Tables. Summary 53 requirements about personnel and equipments were 80% and 87.5% while minimum values were 40% and 25% respectively. Maximum value about quality control measures and waste management were 89.47% and 70% and minimum percentages were 36.40% and 40% respectively. Results have shown that 100% requirements of environmental monitoring and customer care were fulfilled by some labs while some labs only fulfill 20% of these parameters. Conclusion This study shows that in Lahore district veterinary diagnostic labs are not giving proper attention to quality of their system and there is no significant difference between setups of private and public sector laboratories. Availability: Items available for loan: UVAS Library [Call number: 2277-T] (1).

4. Impact Analysis Of Quality Control Practices In Medical Diagnostic Labs Operational In District Lahore

by Tahira Naz Saif (2009-VA-204) | Dr. Ali Ahmed Sheikh | Dr. Ali Ahmed Sheikh | Dr. Muhammad Yasin Tipu.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Medical laboratory services are essential to patient care as laboratory results influence 70% of diagnosis. When tests are performed, there is always some level of inaccuracy. The challenge is to reduce the level of inaccuracy as much as possible. In orders to reduce these errors throughout the lab testing cycle, it is very important to maintain the quality of diagnostic lab. For this purpose standards should be set in the laboratory. These standards have the general requirement for effective management and competent testing. In this study 5 public and 4 private sector medical diagnostic labs in district Lahore were selected. Permission from the competent authorities was taken and information was gathered through questionnaire including parameters such as lab building design, human resource of the lab, equipment and consumables, quality control measures adopted in the labs, environmental monitoring, waste management and customer care covering factors like recruitment policy of staff, Job descriptions of the staff, trainings conducted in the lab, SOPs, quality control and quality assurance program, feedback system, corrective and preventive action implemented in lab, result reporting etc. The data was analyzed statistically through frequency distribution by using Statistical Package for Social Sciences (SPSS) version 18.0 for development of Graphs and Tables. This study was conducted to help to evaluate the quality system of medical diagnostic labs and to identify the critical points which affect the quality of the testing and diagnosis and are being ignored. Information regarding different parameters was summarized in tabulated form by calculating percentage values. Two types of comparison were done. Firstly percentage value for each lab is calculated to check which labs fulfill maximum requirements of the parameters described. Second comparison was done to find out the percentage of the labs out of total selected labs, which fulfill different factors of the parameters described. After statistical analysis using chi square results showed that there is no significant difference between private sector labs and public sector labs. In the first parameter, lab building design lab 1 and lab 9 got 100% quality points and lab 6 and lab 8 got minimum scores of 20%. In second parameter, human resource of lab 1, 2 and 3 got 100% quality points. Lab 6 and 8 got minimum quality points of 33%. When third parameter equipment and consumables was investigated it was found that lab 7 and lab 9 100% fulfill this parameter and lab 6 and lab 8 fulfill this parameter only 16.6%. In fourth parameter, quality control practices implemented in labs; lab 2 got 86% scores and lab 1 got 82% while lab 8 got minimum quality points of 22%. In fifth parameter, waste management of the lab; lab 4 got maximum quality points of 83% and all other labs got the same score of 66.6% in this parameter. When sixth parameter, environmental monitoring was investigated. It was found that lab 2 and lab 9 monitor the environment of the lab only 66.6% and in lab 8 environmental monitoring is not done. In seventh parameter, customer care lab 9 got 100% scores and labs 3, 4, 5 and 6 got 50% scores. Conclusion: Results show that there is no significant difference between government and private sector labs. Labs do not pay attention to the quality control measures and use same approaches of quality control in their system. Availability: Items available for loan: UVAS Library [Call number: 2317-T] (1).

5. Prevalance And Distribution Of Soil-Borne Escherichia Coli O157:H7 In District Lahore Of Punjab Province, Pakistan

by Hiba Tabassum (2011-VA-421) | Prof. Dr. Masood Rabbani | Dr. Ali Ahmed Sheikh | Dr. Sehrish Faryal.

Material type: book Book; Literary form: not fiction Publisher: 2017Dissertation note: Salmonella spp. and Campylobacter spp. (Campylobacter coli and Campylobacter jejuni) are recognized as the leading causes of bacterial gastroenteritis, followed by Shigella spp. and Shiga toxin-encoding Escherichia coli (STEC).(Control and Prevention 2010).Shigatoxigenic Escherichia coli (STEC) include Escherichia coli serotypes whose genomes contain one or more Shiga toxin genes. STEC infections in humans can range from mild selflimiting diarrhea to more severe disease, including hemorrhagic colitis and hemolytic uremic syndrome (HUS). Real-time PCR allows for quantification of the target. Real-time PCR perform better than the standard culture-based assays to detect pathogenic organisms. In summary, work load and work flow issues may dictate which system is best for different-sized laboratories and test volumes.PCR assays to perceive the stx1 and stx2 genes are utilized by several public health laboratories for identification and confirmation of STEC infection. Depending on the primers used, these assays will distinguish between stx1 and stx2 (Zaki and El-Adrosy 2007). Assays even have been developed that verify the specific O group of an organism, detect virulence factors such as intimin and enterohemolysin, and can differentiate among the subtypes of Shiga toxins. So there was need to analyze soil of to check the presence of Escherichia coli O157:H7 for avoiding fatal diseases caused by it and there was also monitored soil chemistry and its relation with bacterial growth specifically Escherichia coli O157:H7 because Different soil composition and different environmental risk factors promoted presence of Escherichia coli O157:H7 in soil. Real Time-PCR technique was opted to detect Escherichia coli O157:H7 in the soil from distant areas of district Lahore of Punjab, Pakistan. Soil samples from 10 per cent Summary 53 villages were collected from this district and handled for genome extraction using commercially available soil DNA extraction kit. After genome extraction, the samples were keep running for Real Time-PCR at optimized conditions. The reaction was improved by variations in standard concentrations of primers, probes, DNA, Taq-polymerase and sequence of primers. The dissemination of soil borne Escherichia coli O157:H7 was mapped in mentioned district of Punjab, Pakistan using geographical co-ordinates recorded by GPS beneficiary. Relationship of Escherichia coli O157:H7 with environmental factors,soil chemistry and source of land irrigation (Canal, tubewell and rain or in combination), was resolved. Results of present project were analyzed through SPSS. The purpose of the research work was the understanding of occurrence and distribution of Escherichia coli O157:H7 in district Lahore of Punjab province .It also threw light on role of soil as a reservoir of Escherichia coli O157:H7, and association of this infectious agent with various risk factors. In this study depending upon the statistical analysis data, it was depicted that Escherichia coli o157 H7 is present in soil although it can’t persist or survive. The prevalence rate of Escherichia coli O157 h7 is 3.1% in 129 soil samples of Lahore. In case of villages it is present in 2 villages of of 29.that shows it is 6.8% prevalent in villages. The presence or absence of pathogen in relation to soil chemistry and seven potential risk factors was determined through student t distribution (T-test). By observing p value of variables of positive and negative sites it comes to know that there is no significant association of these factors to the survival of Escherichia coli O157:H7 in soil. Remaining all other analytes has not significant association with soil. But it can be seen Escherichia coli O157 H7 has significant association with organic matters, phosphorus, Summary 54 copper, cobalt, calcium, sodium, ferrous ion, potassium and sand form of soil ranging from (0.86-1.97), (9.7-22.5), (24.18-41.12), (0.048-0.51), (0.39-0.96), (0.08-0.16), (41.84-59.14), (51.02-69.56), (83-86) respectively. Remaining all other analytes has not significant association with soil. For further investigations it is necessary that find out those factors which cause hindrance in survival of Escherichia coli o157 h7 in soil specifically and also search out those factors which support Escherichia coli O157 h7 persistence in soil. Availability: Items available for loan: UVAS Library [Call number: 2935-T] (1).

Implemented and Maintained by UVAS Library.
For any Suggestions/Query Contact to library or Phone:+91 99239068
Website/OPAC best viewed in Mozilla Browser in 1366X768 Resolution.