1.
Studies On The Prevalence And Taxonomy Of Paramphistomes In Sheep And Their Effects On Various Blood Parameters
by Gohar Zaman Khattak | Dr. Mubashar Saeed | Dr. Asif Rabbani | Dr. Sagheer | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1990Dissertation note: The study was conducted to determine the prevalence of Paramphistomes in sheep, taxonomy of the species of the genus Paramphistomum, and their effects on various blood parameters of the infected animals. For this purpose Municipal Corporation Abattoir, Peshawar was visited regularly during the months of May, June and July,1990. A total of 300 sheep were examined for the study purpose. These animals were divided into two age groups each comprising of 150 sheep. Group-I comprised of sheep below one year of age, while Group-II had sheep of more than one year age. Fifty sheep, each from Group-I and Group-II, were examined each month during the course of study.
Five ml of blood was collected from the jugular vein of the sheep, prior to slaughter, in a test tube having a few drops of 0.1% EDTA. After its slaughter the compound stomach of the animal was obtained and put into a polythene bag, which was then brought to parasitology Section, Veterinary Research Institute, Peshawar for further investigations. After opening of the compound stomach, each compartment was searched for paramphistomes. Helminths so collected were put into glass jars. Preservation and staining of Paramphistomes was carried out by adopting the prescribed methods. Complete record of worms collected, regarding their number and site of predilection was maintained. The taxonomy of the species of the genus Paramphistomum was studied with the help of keys.
All the parasites of the genus Paramphistomum were recovered from the rumen of the infected sheep identified as Paramphistomum cervi.
A total of 2,329 helminths (Paramphistomum cervi) were recovered from infected sheep of both the age groups.Average infection rate in these animals was recorded as 7.00%, with an average worm burden of 111 helminths per animal.
Out of 150 below one year of age sheep examined, 8 were found infected with Paramphistomum cervi with an infection rate of 5.33%. Three sheep from Group-I were found infected with Paramphistomum Cervi in each of the month of May and June, while in July only two animals were found infected with these parasites. The highest rate of infection with these parasites was observed in May and June, and the lowest infection rate was recorded in the month of July. A total of 727 helminths were recovered from the infected animals of Group-I with a range of 73 to 112, and the average parasitic load being 91 per animal.
150 sheep of above one year age were examined,out of which 13 were found infected with Paramphistomum cervi, with an infection rate of 8.66%. Six animals of Group-II were found infected in May, for in June and three in the month of July. A total of 1,602 parasites were recovered from these animals, ranging from 96 to 140, with an average worm burden of 123 helminths per animal.
Blood of infected sheep was exposed to various haematological studies including total erythrocyte count (TEC), Hb estimation, packed cell volume (PCV) and erythrocyte sedimentation rate (ESR).These tests were also carried out for worm-free (control) sheep, 10 each from Group-I and Group-II.
A decrease of 8.39% and 14.20% was noticed in total erythrocyte count (TEC) of the infected sheep of Group-I and Group-II, respectively. The results revealed that a highly significant decrease in the TEC of these animals had occurred due to Paramphistomum cervi infection.
Haemoglobin contents of blood were determined. The results showed a fall in the Hb contents of blood. The results of the study revealed that a non-significant decrease resulted due to paramphistomiasis in the Hb contents of blood of animals with below one year age, while a highly significant decrease occurred in the haemoglobin contents of blood of animals with above year of age.
A decrease of 3.35% and 12.25% was recorded in the packed cell volume (PCV) of the infected sheep of Group-I and Group-II, respectively. The results showed that a non-significant decrease occurred in the PCV of infected animals of Group-I, while a highly significant decrease was caused by paramphistomiasis in Group-II animals.
The erythrocyte sedimentation rate (ESR) of worm free (control) as well as sheep infected with Paramphistomum cervi was determined. The results showed a 14.0% and 141.42% increase in the ESR after one hour in the infected animals of Group-I and Group-II, Respectively, while the increase in ESR after 24 hours was recorded as 120.5% and 196% for these groups, respectively. The results revealed that a significant increase occurred in ESR of infected sheep of Group-I, after one hour as well as 24 hours, a highly significant increase was observed.
Statistical analysis of the results of the study also revealed that there existed a negative correlation between age of the animal and total erythrocyte count, haemoglobin concentration, and packed cell volume of blood. A positive correlation existed between age of animals and erythrocyte sedimentation rate.
Availability: Items available for loan: UVAS Library [Call number: 0004,T] (1).
2.
A Study On The Taxonomy And Incidence Of The Species Of Genus Psoroptese In Buffaloes And Cattle In Lahore
by Asghar Ali | Muhammed Afzal | Asif Rabbani | Iqbal Ahmed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1988Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0023,T] (1).
3.
Application Of Indirect Haemagglutination (Iha) Test For The Confirmation Of Natural Hydatidosis In Cattle
by Ramiz Hussain Farooqui | Mubasher Saeed Mian | Asif Rabbani | Tufail Muhammed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1987Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0026,T] (1).
4.
Survey Of Sheep Trematodes In Lahore Abattoirs
by Majid, A | Altaf Hussain | Asif Rabbani | Muhammed Tufail khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1980Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0028,T] (1).
5.
A Study On Blood Parameters And Comparative Efficacy Of Different Fascioilicial Drugs Against Fascioliasis In cattle
by Mubasher Hussain, S | Asif Rabbani | Mubashir Saeed Mian | Muhammed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1990Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0029,T] (1).
6.
A Comparative Study On Helminths Of Indigenous (Desi) Versus Payoumi Breeds Of Poultry With Taxonomy Of The The Isolated Caecal Nematodes
by Abid Hussain | Asif Rabbani | Muhammed | Muhammid Afzal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1990Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0030,T] (1).
7.
A Study On The Prevalence And Pathogenicity Of Tick Infestation With Taxonomy Of The Genus Rhipicephalus In Dogs
by Azmat Sultana | Mubasher Saeed Mian | Asif Rabbani | Muhammed Sarwer khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1990Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0032,T] (1).
8.
The Study Of Intensity And Chemotherapy Of Echinococcus Granulosus Infection In Dogs In And Around Lahore City
by Farzana Iqbal | Asif Rabbani | Mubbasher Saeed Mian | Tufail | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1985Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0034,T] (1).
9.
A Study Of Gastric Trematodes In Buffaloes And Taxonomy Of The Species Of The Genus Paramphistomum
by Akbar, M | Mubashir Saeed Mian | Asif Rabbani | Muhammed Sarwer khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1989Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0036,T] (1).
10.
Comparative Anticoccidial Activity Of Newly Introduced Coccidiostats Against Experimental Coccidiosis And Their Effects On Grwoth Rate In Broiler Chickens
by Nawaz Malik, M | Altaf Hussain | Asif Rabbani | Iqbal Ahmed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1984Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0077,T] (1).
11.
Study On Gastro-Intestinal Nematodes And Taxonomy Of The Species Of The Genus Haemonchus In Sheep And Goats
by Abid Naseem | Asif Rabbani | Mubasher Saeed Mian | Muhammed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1986Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0100,T] (1).
12.
The Efficacy Of Tetramisole, Mebendazole And Pyrantel Pamoate Against Natural Infectgion Of Toxocara Canis In Dogs
by Shahid Umar, S | Asif Rabbani | Mubasher Saeed Mian | Tufail | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1985Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0118,T] (1).
13.
Study On Hydatidosis And Chemical Characterization Of Cyst Fluid In Camels Slaughtered At Lahore Abattoir
by Tajammal Hussain | Dr. Asif Rabbani | Dr. Iqbal Ahmad | Dr. Mubbasher Saeed Mian | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1988Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0137,T] (1).
14.
A Study On Nematode Parasites And Taxonomy Of Different Species Of The Genus Capillaria In Indigenous Chickens
by Saleem, M | Asif Rabbani | Muhammed | Muhammed Afzal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1989Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0162,T] (1).
15.
Taxonomical Study On Prevalent Ectoparasites & The Effect Of Tick Infestation On Various Blood Parameters
by Nisar Ahmad | Asif Rabbani | M.Sarwer khan | Muhammed Afzal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1993Dissertation note: A study was conducted to see the prevalence and to identify the species of ectoparasites (Ticks, mites, fleas, lice) in dogs.
Samples were collected from College of Veterinary Sciences and different pet clinics in the city. Specimens were collected and then transferred into the glass bottles containing 50%, 60%, 70%, 80%, 90%, alcohol for preservation.
The specimens were examined and identified in the laboratory.
The species of ectoparasites identified were: Rhipicephalus anguineus (tick), Sarcoptes scabiel Var canis (mite), Ctenocephalides Canis (flea) Trichodectes anis (lice).
The percentage of the above mentioned species were: 39.3%, 11.1%, 2.1% and 2.1% respectively.
Haematological examination were made to compare the blood picture of healthy and infested dogs. For this purpose 20 blood samples each from healthy and infested dogs were examined. The blood parameters studied were: Haemoglobin level, total erythrocyte count, total leukocyte count, packed cell volume and differential leukocyte count. The results of haemotological examination showed a significant decrease in haemoglobin, total erythrocyte count, and packed cell volume, whereas a significant rise in leukocyte count and D.L.C. of infested dogs as compared to healthy dogs was observed.
Availability: Items available for loan: UVAS Library [Call number: 0213,T] (1).
16.
Effects Of Natural Dicrocoeliasis On Different Blood Parameters & Serum Bilirubin Level In Sheep
by Alia Bano | Mubasher saeed Mian | Asif Rabbani | Manzoor Ahmed Basra | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1991Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0235,T] (1).
17.
A Study Of The Taxonomy & Bionomics Of Genus Haemaphysalis In Domestic Animals
by Haroon, Durrani | MubasherSaeed Mian | Asif Rabbani | Muhammed sarwer Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
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not fiction
Publisher: 1992Dissertation note: Ticks transmit diseases like piroplasmosis, theileria3is and anaplasmosis in livestock. They are responsible for great economic losses in terms of general unthriftiness, loss of condition and also damage to hides and skins. Pakistan being a tropical country provides optimal conditions for growth and multiplication of ticks. Despite this abundance in prevalence little systematic work was done to investigate the prevalence, taxonony and bionomics of species of genus Haemaplxysalis.
During this study numerous ticks from sheep, goat, cattle and buffaloes each were collected from Lahore and Sheikhupura districts. One species of genus Haemaphysalis i.e. Haemaphysalis brunati was recorded. Other species of same genus did not occur in these districts. This species was recorded from Lahore only. The percent incidence was 5.87%.
During the bionomical study various aspects like seasonal and regional abundance, life cycle, effect of temperature and humidity were studied. It was concluded that in one year three generations of Haemaphysalis bnmati occured in Lahore district . The ticks appeared on livestock in Narch and gradually increased during following months and reached its peak level in June. Then the population graph fell, until in last week of September when a minor peak was recorded. Subsequently the infestation receded considerably amongs livestock during October and was lowest in the end of November. No incidences were recorded from early December through late February.
During the morphological studies of Haemapltysalis brunati measurements of various parts of body were recorded and it was noticed that the measuremeni vary slightly in the species occuring in Lahore
The breeding habits and life cycle of Haemaphysalis brunati were studied under natural conditions and it was observed that the ticks mostly started depositing egg 19 days after dropping in spring, 18 days after dropping in summer and 7 days after dropping in autumn.
The oviposition period under natural conditions was found to be 12 days in spring, 11 days in summer and 11 days in autumn.
The average number of eggs laid by a single female tick in different seasons was not constant. In spring season average number of eggs deposited by single female tick was 3819 in summer 2786 and in autumn 2514. The eggs were oval in shape and measured 0.470 x 0.420 mm in size and weighed 0.041 mg on average. The incubation period of ova was found to vary in different seasons. In spring it was 20 days, 16 days in summer and 15 days in autumn.
The larvae survived without food and water for 56 days in spring. The larvae fully engorged in 9 days during spring, 7 days in summer and 6 days in autumn. The weight of unfed larvae was found to be 0.375 mg. and engorged larvae from 0.166 nlg. The total amount of blood sucked was estimated to be 0.129 mg. The fully engorged larvae moulted in 16 days during spring, II days during summer and 10 days during autumn. The newly moulted unfed txymph lived for 65 days in spring. The nymph engorged in 7 days during spring, in 6 days during summer and autumn. The weight of unfed nymph was found to be 0.114 mg and that of. engorged nymph was found to be 1.094 mg. The nymph moulted in 17 days during spring and in 9 days during summer and autumn.
The newly moulted female engorged within 9 days in spring, 8 days in summer and 4 days in autumn. The weight of unfed female was 1.695 mg and of engorged female 210.0 mg. The amount of blood sucked by a single female tick was estimated to be 208.36 mg. Amount of blood sucked by a single female during her entire life span was found to be 221.549 mg.
Influence of temperature and humidity on rate of development of Haemapbysalis brunati on preoviposition period, oviposition period, number of eggs laid, incubation period and peientage of hatchability were studied and it was observed that constant temperature and varying humidities had no significant effect on the said parameters whereas constant humidity and varying temperatures had a significant effect on the rate of development, preoviposition period, oviposition period, number of eggs laid, incubation period and percentage of hatchahility.
Availability: Items available for loan: UVAS Library [Call number: 0245,T] (1).
18.
Haematological Studies, Serum Bilirubin Determination, Chemotherapy And Incidence Of Fascioliasis In Naturally Infected Sheep
by Ajaz Saleem, M | Mubasher saeed Mian | Asif Rabbani | Iqbal ahmed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1985Dissertation note: A study was conducted to find out the haematological changes, serum bilirubin level, relative efficacy of the three drugs, i.e., Coriban (Wellcome), Bilevon-M (Bayer) and Acedist (Ciba) and incidence of fascioliasis in naturally infected sheep. The study was made from the first week of January to the last week of March, 1985.
Haematological changes were observed after examining the Total Erythrocyte Count, Total Leucocyte Count, Differential Leucocyte Count, Packed Cell Volume and haemoglobin estimation of 80 naturally infected and 20 healthy sheep in Lahore. It was concluded that there was a drop in erythrocyte count, packed cell volume and haemoglobin concentration in the infected sheep and a normocytic normochromic type of anemia was detected. Besides this, it was also noticed and recorded that there was a rise in the total serum bilirubin level of the same 80 infected sheep which were naturally infected with fascioliasis were divided into four groups A, B, C and D of 20 animals each for the study of the efficacy of three antifascioliatic drugs (Coriban, Bilevon-M and Acedist) at manufacturer's recommended dose rates afterwards. Each group was given a separate drug on the Zero day and the fourth group (D Group) was left untreated as a Control At the end of experiment on the 28th day, the efficacies recorded were 98.86%, 97.67% and 97.70% for Coriban, Bilevon-M and Acedist respectively. Statistically there was no significant difference between the three drugs but a significant difference between the replications was noted by applying analysis of variance. An increase in the daily average weight gain of 48.21, 35.71 and 39.28 grams were recorded in A, B and C groups respectively and by applying paired 't' test it was proved that there was a significant difference between the weight gains of the animals treated with these drugs.
100 livers along with gall bladders were randomly selected from different abbatoirs of Lahore during the months of January, February and March to study the incidence of fascioliasis in sheep. An incidence of 8% was recorded for Fasciola hepatica and 4% for Fasciola gigantica, with an overall percentage of 12. for fascioliasis in sheep.
Availability: Items available for loan: UVAS Library [Call number: 0258,T] (1).
19.
Study On Incidence And Chemotherapy Of Gestode Infection In Dogs At Lahore
by Faizan Baig, Mirza | Mubashir Saeed Khan | Asif Rabbani | Tufail Muhammed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1985Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0263,T] (1).
20.
Comparative Studies On The Efficacy Of Indirect Haemagglutination Test (Iha) And Double Immuno Diffusion Test (Did)
by Zulfiqar, M | Mubasher Saeed Mian | Asif Rabbani | Khalid Pervaiz | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1985Dissertation note: One hundred faecal samples, 100 blood samples and livers including bile ducts were collected from sheep suffering from Fascio1iasis slaughtered at Lahore and Sheikhupura abbatoirs.
Faecal examinations were carried out with fresh smear and centrifugal techniques and only 35 animals were found passing Fasciola ova. Serum was prepared from the blood samples collected from infested sheep for performance of indirect hacinagglutination test and double imniuno-diffusion test. Crude antigen was prepared from the fresh liver flukes specimens collected from infected livers which contained 4.5 mg protein/mi. Hyper immuneerim was prepared in rabbits by inoculating antigen. Double immuno-diffusion test was performed on glass slides coated with 1% agar Gel and. incubated at 37 C for 48 hours. The positive reactions showed diffuse lines. 45 sheep were found positive with Double immuno-diffusion test.. For performing the indirect haemagglutination test fresh red blood cell of the healthy sheep were stabilized with gluteraldehyde and sensitized with Tannic acid. Crude antigen was attached to these cells at pH 6.4 which were used in Indirect haemagglutination test. Serum samples were tested with Fasciola antigen in "V" plates of microtiter sptem. The end points of the plates were read after 16 hours a room temperature. Sora were prediluted 50 times but agglutination was seen even at 1/100 dilution. In positive reactions cells were like mat of cells in the bottom of the wells In negative reactions, cells settle down and looked like button in the center. 59 animal were found positive with indirect haemagglutination test. While comparing the sheep serum samples results with hyper immune serum collected rom rabbits it was found that all the positive sheep serum samples coincide with the results of hyper immune serum.
From the conclusion of the results it was found that indirect haemagglutination test was more sensitive (59%) than double immuno diffusion test(45%)as compared with the coprological examination 35%
Availability: Items available for loan: UVAS Library [Call number: 0272,T] (1).
21.
Comparative Efficacy Of Trypanocidal Drugs And Their Effects On Some Blood Parameters I Camels Naturally Infected With Surra
by Farrukh Jamal Nizami | Muhammed Afzal | Asif Rabbani | Muhammed Sarwer Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
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Publisher: 1993Dissertation note: Trypanosomiasis in camels caused by Trypanosoma evansi results in great economic losses if not treated properly. The present study had been designed to find out the prevalence and treatment of Trypanosoma evansi infection by using Naganol (sayer) and Trypacide (M & B) in camels in District Layyah. The effects of the infection and drugs on differential leukolyte count (DLC), Haemoglobin (HB) Packed Cell Volume (PCV), Erythrocytic Sedimentation Rate (ESR) and Total Serum protein were also estimated. For this purpose 1000 suspected camels were examined and 75 (7.5%) were found positive for Trypanosoma evansi infection. Out of these, 45 positive cases were randomly divided into 3 equal groups comprising 15 animals each. Two of the groups were served with two different drug trials, and the 3rd group was kept as untreated infected control. While a 4th group of 15 camels was kept as non-infected and untreated control. The results of all the aforesaid blood parameters were recorded just before and on day 21 post-treatment. The data so obtained was analysed and results were made as follows.
Out of 1000 camels, 75 were found to be harbouring the Trypanosoma evansi infection with a pevalence rate being 7.5 percent.
Naganol (Bayer) was found to be 60% Trypanocidal on day 5 and 100% on day 10 and 21 post treatment. Trypacide (M & B) was found to be 53% Trypanocidal on day 5 and 80% on day 10 and 21 post treatment.
There was marked Neutropenia with lymphocytosis before the treatment in infected versus non infected ones. The average values of Neutrophils were 38+0.62, 39±0.02, 36±0.47 and 52j0.48 in the 4 groups respectively. But on day 21 after treatment, the number of neutrophils increased in group Nos. I and II with n average of 51±0.33 and 49±1.41 while the group No. Ill and IV revealed no difference in their count. The mean values of lymphocytes prior to and after the treatment were 51±0.33, 52±0.71, 56±0.46 and 41±0.36, 42±0.40, 45.3±1.09, 56±0.46 and 39±0.23 respectively. There was 41 .66% increase in Eosinophils of group I, II and Ill before the treatment, the average being 7±0.22, 50.48 and 5±0.48 respectively, while the group No. IV possessed 4+0.19% neutrophils. The Eosinophilia tended to become normal with 33.33% decrease, average being 4+0.40 and 3+0.45 in group No. I and II respectively on day 21 post treatment; while the neutrophils count remained nearly the same in group No Ill and IV average being 6+0.24 and 4+0.19 respectively on day 21 post treatment.
The Basophils and Monocytes Count remained unchanged before and after the treatment in all the 4 groups.
Haemoglobin(Hb) and Packed Cell Viume (PCV) showed decrease on day 0, but Erythrocytic Sedimentation Rate (ESR) and Total Serum Protein were increased on day 0 in case of positive camels. On day 21 post treatment with Naganol (Bayer) and Trypacide (M & B), Haemoglobin (HB) and Packed Cell Volume (PCV) were increased in group Nos I and II. Whereas Erythrocytic Sedimentation Rate (ESR) and Total Serum Protein level were decreased on day 21 post treatment in the same groups. On the other hand the values remained unchanged in group No. IV. (Noninfected, untreated control).
Availability: Items available for loan: UVAS Library [Call number: 0283,T] (1).
22.
A Study On The Taxonomy & Prevalence Of The Species Of Genus Ancylostoma & Effects On Natural Infections On Various
by Kamran Ashraf | Mubasher Saeed Mian | Asif Rabbani | Muhammed Sarwer Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1993Dissertation note: The study was conducted to determine the prevalence of Ancylostomes (Hookworms) in dogs, taxonomy of the species of the Genus Ancylostoina and their effects on various blood parameters of the infected canines.
For above purpose, samples were collected from dog hospital, College of Veterinary Sciences and different pet clinics in the city.
Faecal and blood samples were stored in the refrigerator, of Parasitology Laboratory.
Only those dogs which had high prevalerit in Ancylostomes were examined by different faecal examination methods, were selected to get mature ancylostomes, after killing the dogs. Preservation and staining of Ancylostimes was carried out by adopting the prescribed methods. The taxonomy of the species of the genus Ancylostoma was studied with the help of keys.
The collective over all prevalence of different types of Ancylostomes recorded in stray and pet dogs was 28%.
The species of Ancylostomes identified in our area were: Ancylostoina Caninum, Ancylostoma braziliense and Ancylostoma duodene1e.
The percentage of the above mentioned species were 100%, 33% and 2.5% respectively. Blood of infected dogs were exposed to various haematological studies including Hb estimation, erythrocyte sedimentation rate (ESR), packed cell volume (PCV), total erythrocyte count (TEC), total serum protien level (TSP) and differential leukocyte count (DLC).
liaematological examinations were made to compare the blood picture of healthy and infected dogs. For this purpose 20 blood samples each from healthy and infected dogs were examined.
The results of haematological examination showed a significant decrease in haemoglobin estimation total erythrocyte count, packed cell volume and total serum protien level, whereas a significant increase in erythrocyte sedimentation rate and differential leukocyte counts, like neutrophils, eosinophils and very little increase in basophils were also seen.
Availability: Items available for loan: UVAS Library [Call number: 0284,T] (1).
23.
Taxonomic And Therapeutic Studies Of Natural And Experimental Coccidiosis In Commercial Quails
by Azhar Iqbal | Asif Rabbani | Muhammed | Muhammed Afzal | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1991Dissertation note: Taxonomic arid therapeutic studies of natural and experimental coccidiosis in commercial quails were undertaken. Total 500 gut samples were examined microscopically to study the incidence of cocoidiosis, which was recorded as 69%. By making taxonoinical study based on size, morphological characteristics, sporulfttion, time and post-mortem lesions it was concluded that the causal coocidia was L bateri. Average size of the oocyst recorded was 21.42 u x 14.28 u while sporulation time recorded was 24 hours at 27°C. The cocysts were double walled and 1.2 u in thickness. Oocysts production per grain of faeces in natural infection ranged between 31-350 oocysts. While average number of oocysts per gram of faeces recorded was 220.
To study effects of ESB3 and Darvisul AK plus on experimental infection one hundred and sixty day old quail chicks were reared upto 3 weeks of age in coccidia free environment. They were then randomly divided into four groups A, B, C and D. Comprising of 40 birds each these groups were sub-divided into four replicates each comprising of 10 birds. Birds of group B, C and D were infected with ml of coccidial inoculum having 50,000 sporulated oocysts given directly into crop. On fourth day post infection birds of' all infected groups showed disease symptoms and at that time groups C and 0 were treated with darvisul AK plus and ESB3 respectively while group B was kept as infected and un-medicated group. At the end of experimental period in 6th week maximum weight was gained by group "A" and minimum by group "B" Group 0 which was treated with ESB3 gained less weight than group C which was treated with Darvisul AK plus.
Best feed conversion ratio was seen in group A and among the medicated groups group 0 revealed better feed conversion ratio than group C, while group B showed the worst feed conversion ratio.
Highest mortality of 40% was recorded in group B, 10% in group C and least in group D i.e. 2.5%.
From the findings of the study it was concluded that our commercial quail is suffering from coccidiosis caused by E. bateri and ESB3 is an affective remedy for this disease. However it is suggested that more work to elucidate the effects of ESB3 with additional vitamins A and K be carried out.
Availability: Items available for loan: UVAS Library [Call number: 0343,T] (1).
24.
Efficacy Of Coxeva In Experimental Caecal Coccidiosis And Its Effects On Body Weight In Broiler Chicks
by Yaqub Malik, M | Mubasher Saeed Mian | Asif Rabbani | Muhammed Sarwer Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: The study was conducted to evaluate the efficacy of anticoccidial drug coxeva (a mixture of 80% sulphadimidine and 8% diaveridine) and its effects on body weight, growth rate and feed convertion ratio were studied. For this purpose 120 day old broiler chicks were reared upto the age of 4 weeks. At the end of 4 weeks the birds were randomly divided into four groups A, B, C, and D, each group containing 30 chicks. The birds of each group were weighed. The group B, C and D were infected with 50,000 sporulated oocysts of Eimeria tenella. While the group A was left uninfected and kept as control on the 6th day after the appearance of clinical symptoms of caccal coccidiosis, the group C and D were medicated with coxeva (the group C for five continuous days and group D for three days on 2 days off three days on), the group B was left as infected control. The daily oocysts count per gram of faeces were taken upto the end of experiment which was zero in group C on 10th day and in group D on 13th day the oocysts were at their peak (5,20,000) on 15th day of inoculation. The body weight was recorded at the end of each week throughout the experiment which showed significant difference in group B and group A, C and D . The feed consumed by each group was recorded on daily basis and growth rate was recorded at the end of each week. The analysis of variance revealed significant difference in feed consumption and growth rate in group B and other groups. The feed conversion ratio was recorded for each group at the end of each week which showed non significant difference among group A, B, C and D. The drug was also evaluated for its modes of administration (continuousand intermittent) and found no significant difference in both modesof administration. The mortaility was recorded which was five birds (16%) in group B. The drug proved hundred percent effective against caecal cocidiosis in broiler chicks.
Availability: Items available for loan: UVAS Library [Call number: 0345,T] (1).
25.
A Comparative Study Of Gastro-Intestinal Helminths In Desi (Indjegenous) & Commercial Layers With Taxonomy Of The Isolates
by Saleem Khan, M | Dr. Asif Rabbani | Dr. M. Sarwar | Dr. Mubashar Saeed Mian | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1991Dissertation note: The present project was designed to estimate and compare the extent of helminth infestation in Desi (indigenous) and commercial (W.L.H) poultry layers along with taxonomi study of the isolated species.
A total number of six hundred (600) gut samples of adult layers i.e. three hundred (300) each of desi (indigenous) and commercial (W.L.H) were collected from June through october 1991. The parasites were recovered from the gastrointestinal tract of birds by adopting described method at the Parasitology Laboratory College of Veterinary Sciences Lahore.
The overall prevalence of helminth parasites (netnatodes and cestodes) was 80.3% in desi and 32.6% in commercial (W.L.H) birds.
The nematodes were found in 210(70%) in desi (indigenous) and in 62 (20.6%) of commercial (W.L.H) guts. Among the nematodes different species were identified and their infection rate on single and/or multiple basis was established. Ascaridia galli was the most prevailing species 167 (55.6%) followed by Heterakis allinae 38(12.6%), Subulura bruinpti 45 (1b.k) wniie iiojua trachea was very rare and was present only in 5 (1.6%) of desi (indigenous) birds.
In commercial (W.L.H) layers the nematodes singly and/or on multiple basis were recorded in 98(32.61) of birds Ascaridia alli alone infected 49(16.3%), HtrJd. gallinae 7 (2.3%) and Subulura brunipti 8 (2.6%) of birds while Svnainus trachea was very rare and present only in 2 (0.6%) of the tracheas.
The overall incidence of cestodes was slightly higher than those of nematode parasites. It was found to be 65.0% and 22.6% in desi (indigenous) and commercial (W.L.H) layers respectively.
The species of cestode with percentage of infection recovered from the small intestine of desi (indigenous) birds were:
Raillietina tetraona 58.0% (174/300)
Raillietina cesticillus 12.6% (38/300)
Raillietina echinobothrida 14.0% (42/300)
Cotugnia digonoora 10.8% (32/300)
Choanotaenia infundibulumn 20.0% (60/300)
Amoebotaenia sphenoides 3.3% (10/300)
While in conimercial (W.L.H) layers
Raillietina tetraona
Raillietina cesticillus
Raillietina echinobothrida
Cotunia dionopora
Choanotaenia infundibuluni
Anioebotaenia sphenoides
they were:
22.0% (66/300)
1.0% (3/300)
3.0% (9/300)
1.0% (3/300)
4.3% (13/300)
2.0% (6/300)
No trematode parasites was however recovered both in
desi (indigenous) and commercial (W.L.H.) birds during the
present study.
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26.
Serodiagnosis Of Ovine Hydatidosis
by Javaid, M | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1993Dissertation note: A study was under taken to find out the incidence of hydatidosis and to evaluate the efficacy of indirect haemagglutination (IHA) test for the confirmation of natural hydatidosis in sheep slaughtered at Lahore municipal abattoir.
Blood samples from 200 sheep (50 each from hydatidosis affected and free sheep on the basis of postmortem findings and 100 blood samples Elected randomly without considering postmortem finding). The serum from each sample was separated, properly labelled and stored at -20°C.
For the preparation of antigen, crude cyst fluid was aspirated aseptically from hydatid cysts. Blood from healthy sheep was collected in 3.8% sodium citrate solution and red blood cells were separated by centrifugation. A 2.5% red cell suspension was prepared in Phosphate Buffered Saline (PBS). The sheep erythrocytes were sensitized by Hydatid fluid antigen. Optimal dilution of antigen 1:16 was used in Phosphate Buffered Saline (p11 6.4) for sensitizing the sheep erythrocytes. All the sera were inactivated at 56°C for half an hour and serial two f1d serum dilutions were prepared by micropipettes in microtitre U plates and sensitized erythrocytes were added to the plates and incubated at room temperature in a humid chamber for 3 hours. A titre of 1:32 and above was considered as positive. In positive reactions, the cells agglutinated like a carpet at the bottom of the wells where as in negative cases the cells settled as a compact mass in the centre of the wells. By the indirect haemagglutination test Ninety-two percent sheep were found positive for hydatidosis. (Table-4.2). Out of 50 serum samples (Group A2), 46 (92%) were confirmed positive on postmortem while 3 out of 50 (6%) hydatid free samples (Group A3), gave false positive results with IHA test.
It was concluded that indirect haemagglutination test is an accurate, reliable and sensitive test (92%) for the diagnosis of hydatidosis in sheep.
The blood cell counts (TLC, DLC) and blood chemistry (Total protein, A/G ratio) of the samples under investigation were also carried out. From the results it was evident that the hydatid cysts did not affect the blood values of the host significantly. However, only 28% of hydatid positive animals showed eosinophilia ranging from 7 to 23% which was non pathognomonic.
It was thus inferred that blood cell counts and blood c1vmistry of the hydatid cyst patients was of no diagnostic value.
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27.
A Comparative Study Of Lungworms In Sheep & Goats & The Effect Of Mixed Namatode Infection On Certain Blood Parameters
by Irshad Ahmed Sherazi | Dr. Sh,. Asif Rabbani | Dr. Muhammad | Dr. Muhammad Afzal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: The present study was conducted to find out the incidence of lungworm infestation, identify the different species and to observe the effect of mixed nematode infections on certain blood parameters in sheep and goats. The study was conducted at Parasitology Laboratory, College of Vety. Sciences Lahore. For this purpose sheep & goats brought to Lahore abattoirs for slaughtering were closely examined before and after slaughter and animals suspected for lungworms were selected for collection of blood and tissue samples. 200 samples of lungs and blood 100 each from sheep and goats were collected for parasitological and haematolog ical examination, respectively.
The results revealed that incidence of lungworms infestation in sheep and goats was 31 and 11 %, respectively. It was observed that Dictyocaulus filaria, Protostrongylus rufescence and Muellerius capillaris infection in lung of sheep was in the order of 22, 8, and 1 % respectively, whereas, the incidence of lungworms infestation in lungs of goats caused by the same species was 8, 3 and 0 %, respectively.
The highest incidence of lungworms infection observed was that of Dictyocaulus filaria in both the sheep and goats, while Muellerius capillaris was found only in lungs of one sheep and infection by this species could not be detected in goats.
The findings of this study showed that average red blood cell count, heamoglobin level and packed cell volume were reduced due to nematode infection in sheep and goats. The results have also indicated that nematode infection in sheep and goats enhanced erythrocyte sedimentation rate. From the results of the present study, it may be concluded that incidence of lungworm infestation in sheep and goats is on the higher side which warrants that suitable treatment and control measures should be adopted to safeguard our valuable livestock from this serious infection. It is also evident from the results that nematode infection adversly affects the components of blood in sheep and goats which may lead to anaemia and loss of growth and production.
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28.
A Study On The Prevalance Of Ascaridia Galli And The Effects Of Experimental Infection On Various Blood Parameters
by Rubina Rehman | Dr. Mubashir Saeed Mian | Dr. Asif Rabbani | Dr. M. Athar | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 1993Dissertation note: Ascaridia galli is the common intestinal nematode of poultry that causes severe losses in birds resulting in decreased weight gain and fall in egg production. These symptoms show that parasitic diseases are the constraints in the development of profitable poultry industry.
To find out the prevalence 300 chicken guts were obtained from different poultry meat shops in Lahore from May to August, 1993.
An overall prevalence of 60 percent was recorded in chicks. The incidence of infection was highest (73.3 percent) in the month of July and lowest (42.6 percent) in the month of May.
For conducting haematological examination one hundred and thirty-five chicks were purchased and reared under good hygienic conditions. At the age of 15 days chicks were divided into 3 groups (A, B and C). Chicks of groups A and B were infected with 50 and 100 eggs, respectively, while group C was taken as control. After 35 days of inducing experimental infection blood samples from all the three groups were taken at an interval of 5 days i.e., 35, 40 and 45 days postinfection to examine erythrocyte count, packed cell volume, haemoglobin level, total leukocyte count and differential leukocyte count.
Significantly lower values of erythrocyte count, packed cell volume and haemoglobin were estimated in infected groups A and B as compared to control group C, while higher values were obtained for heterophil, lymphocyte and eosinophil in infected chicks resulting in an overall increase in total leukocyte count. It was also estimated that A. gal/i produced no significant change in monocyte count.
Chicks from group A, B and C were weighed every week after infection to see the effect of A. gaili infection on body weight.
Results regarding body weight showed that there was significant decrease in weight in group A and B as compared to control group C and there was also significant decrease in group B as compared to group A.
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29.
A Study On The Taxonomy Of Sheep Cestodes And The Efficacy Of Albendazole And Miclosamide Against Natural Infections
by Ashraf, M | Dr. Asif Rabbani | Dr. Mubashar Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: Pakistan has a large population of live stock, which plays an important role in the economy of the country. Parasitism adversally effects the growth and production of livestock. Great economic losses have been attributed to the sheep population in our country due to the parasitic infections including cestodes. A study of cestodal infections with taxonomy of the prevalent cestoctes species affecting sheep at different age groups in and around Lahore was conducted. The taxonomical study was conducted in the month of August, September and October, 1993. For this purpose 200 guts (one hurtdered each from below six month and above six month of sheep) were collected from Lahore Abattoir. Parasites were identitied under the microscope by preparing permanent mounts.
The over all incidence of cestodes infection in sheep was 65.5%.
The species of various genera of cestodes observed during the present
study were:
Moniezia pansa (64%), Moniezia benedeni (60%), Avitellina cjj[iur1ctta
(49.50%), Avitellina lahorea (40%), Avitellina sudanea (30%), Stilesia vittata
(30%), Stilesia globiounctata (50%) and Thvsanosoma actinioides (6%).
Monthwise prevalence of cestodes infection was also carried out and it was observed that the highest infection rate was (76%) during the month of August and (66.25%) during the month of September. While the lowest incidence was recorded as (57.14%) during the month of october, 1993.
The taxonomy of the prevalent cestode species was carried out. The effect of age of the animals on the intensity of cestodal infection was also studied. There was a significant difference in the prevalence of cestode infections among the two groups.
The present work was also aimed to verify the efficacy of two commonly available anthelmintics namely albendazole (valbazen) and niclosarnide (mansonil), in naturally infected stceep with Gastrointestinal cestocJes. Fifty sheep positive for cestods infections were randomly divided into two groups A and B, each group comprising of 25 animals. Group. A was given albendazole (valbazen) at a dose rate of 2.5 mg/kg body weight orally while the group B was administered niclosamide (mansonil) at a dose rate of 100 mg/kg body weight orally. The efficacy of both the drugs was evaluated on the bases of reduction of segments and number of eggs per gram of faeces after medication. The egg counts were made on zero, 3rd and 21st day. Both the drugs revealed a progressive decrease in the faccal egg/segment counts. The efficacy of albenclazole on 3rd and 21st day was 96.02 and 99.23% repectively while the efficacy of niclosamide (mansonil) was 100% on 3rd and 21st day.
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30.
A Comparative Study Of Helminth And Haemoparasites Of Domestic And Wild Pigeons
by Asma Hussain | Dr. Mubashir Saeed Mian | Dr. Asif Rabbani | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1995Dissertation note: There is an increasing interest in pigeons and other game and ornamental birds. These birds are generally kept either free roaming or confined in outdoor pens and hence are vulnerable to various parasitic infectious, which greatly effects the productivity of these birds.
The meat production of the pigeons can be improved by controlling disease problems particularly helrninths and blood protozoan infections, so that in near future they may contribute towards narrowing down the animal protein supply gap by substituting poultry meat with squabs.
The present work was planned for the comparative study of helminths and heemoparasites of domestic and wild pigeons. For this purpose 300 each of guts and blood smears were studied. The overall incidence of gastrointestinal helminths and blood protozoans was 77.33% and 31.99% respectively, while 36% of birds had mixed infection. The incidence of gastro-intestinal helminths and blood protozoa in wild pigeons was 89.33 and 20.66 percent respectively and in domestic pigeons it was 65.33% and 11.33% respectively. Whereas mixed infections were 22% and 14% respectively.
The following species of helmitiths and blood protozoa were recorded and identified.
1. Raillietina tetragona
2. Raillietiiia cesticillus
3. Choanolaenia infundibulum
4. Ascaridia colurnbae and
5. Cap illaria obgnata
The two species of blood protozoa recovered were:
1. Aegypanella pullorum and
2. Haeiçotuscumbae
Among the helrninths recorded, cestodes were found predominating as compared with nernatodes in both wild and domestic pigeons. Raillietina cesticillus was the most common cestode species in both Wild and domestic pigeons i.e. 51 and 33 percent respectively while Asci colurnbae predominated the nernatode species i.e. 18 and 15 percent respectively. The Incidence was higher in wild pigeons.
Aegyptianella pullorum was more common blood protozoan i.e. 22 and 11 percent respectively in wild and domestic pigeons. The incidence of Haemoproteus clumbae was 9 and 11 percent respectively.
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31.
The Effects Of Piroplasmosis On Hematology In Equines
by Salva | Dr. Mubasher Saeed Mian | Dr. Asif Rabbani | Dr. Shakeel | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1996Dissertation note: Piroplasmosis is a serious disease of the domestic animals including horses. It is caused by species of the genus Babesia i.e. . egui and B. caballi resulting in death due to excessive loss of blood. The present study was conducted to find out the effect of natural Piroplasmosis on various blood parameters, viz, total erythrocytes count (TEC), hemoglobin estimation (Jib), packed cell volume (PCV), total leukocytes count (TLC) and differential leukocytes count (DLC) in horses.
A total of forty horses (Group A including 20 uninfected horses and Group B included 20 horses showing clinical symptoms of the disease and confirmed positive for piroplasmosis through blood examination were used in the study and found to cause the infection although . caballi infection was more predominant.
Heamatological examination revealed a significant decrease (P<0.05) in the erythrocyte count, hemoglobin level and packed cell volume of the infected horses.
Normocytic and hypochromic type of anemia was observed. Total leukocyte count was also decreased. A decrease in the number of rieutrophils, eosinophils, basophils, monocytes and lymphocytes was also observed in the infected horses.
Out of the 20 infected horses, 4 had B. equi and sixteen had B. caballi infection indicating that . caballi was more prevalent.
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32.
A Study Of Gastro Intestinal Parasitism And Haematological Disturbances Associated With Single Or Multiple Infection In Sheep
by Hafeez, M | Dr. Asif Rabbani | Dr. Mubashar Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1996Dissertation note: Nature has blessed Pakistan with a large population of livestock which play an important role in the economy of the country. Parasitism adversely effects the growth and production of livestock. Great economic losses have been attributed to the sheep population in our country due to parasitic infections including gastro-intestinal parasitism. A study of gastro-intestinal parasitism with isolation and identification of the parasite species affecting sheep at different age groups in and around Lahore was conducted in the months of July, August, September and October, 1994.
For this purpose 200 G.I. tracts of sheep (One hundred each from below six months and above six months of age) were collected from Lahore Metropolitan Corporation, Abattoir.
The overall incidence of gastro-itestinal parasitism in sheep was found to be 68.5%. Age group wise incidence was found to be 71% and 66% in sheep below and above six months of age respectively. Classwise overall incidence was found to be Sporozoan (51%), Trematodes (21%), Cestodes (62%) and Nematodes (68%).
Age group wise incidence of Sporozoan, Trematodes, Cestodes and Nematodes was found to be 60% and 42%, 12% and 30%, 71% and 53%, 73% and 63% in sheep below and above six months of age.
Month-wise prevalence of gastro-intestinal parasitism was also carried out which was higher in the month of August and lowest in the month of October. Nineteen different species of Parasites were recorded which are detailed as under:
1. Eimeria arloingi
2. Eimeria ninakohlvakimovae
3. Eimerla parva
4. Elmerla intricata
5. Eimeria faurei
6. Paramphistomum cervi
7. Cotylophoron cotvlophorum
8. Moniezia. expansa
9. Moniezia benedeni
10. Avitellina centripunctata
11. Haemonchus contortus
12. Oesophagostomum colunThianum
13. Oesophagostonim venulosum
14. Trichuri
15. Ostertagia circumcincta
16. Ostertagia ostertagi
17. Chabertia ovina
18. Trichostrongvlus colubriformis
19. Nematodirus spathiger
The isolation and identification of various collected parasites was carried out. The relationship between the age of sheep and the occurrence of gestro-Intestinal parasitism was also studied. There was a Non Significant difference in the prevalence of gastro-intestinal parasites among the two groups.
The present project was also aimed to observe the effect of single or multiple parasitism on certain haematological parameters like, estimation of haemoglobin contents, total erythrocytic count, total leukocytic count, erythrocyte sedimentation Rate, packed cell volume and differential leukocytic count.
These heamatological studies were carried out on control (parasitic free) and parasitised sheep of below and above six months of age.
The findings of haematological studies showed that there was decrease in haemoglobin contents. total erythrocytic count, packed cell volume, total leukocytic count and increase in erythrocyte sedimentation rate in both sheep below and above six months of age under the effect of single or multiple gastrointestinal parasitism. The values of differential leukocytic count were variable in both age groups.
Most of the haematological disturbances on various blood parameters in both age group of sheep were proved to be statistically significant.
From the results of the presents study, it has been concluded that the incidence of gastro-intestinal parasitism in sheep below and above six months of age is on the higher side which warrants that certain essential preventive measures must be adopted to safeguard our valuable livestockfrom these serious parasitic infestations. It is also evident from the results that gastro-intestinal parasitism adversely affects the blood components in sheep which may lead to anaemia, loss of growth and loss of production.
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33.
A Study Of Gastro Intestinal Helminthiasis And The Effects Of Natural Infection On Various Blood Parameters In The Buffaloes
by Ishtiaq Ahmad | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1995Dissertation note: A total of 76 buffaloes brought to the Lahore Metropolitan Slaughter house were studied for helminthiasis. Out of which 50 (65.79%) were found positive.
The helminthiasis positive guts were examined for the presence of helminths and the following species were identified Haemonchus contortus (11.84%), Haemonchus placei (10.53%), Ostertagia ostertagi (7.89%), Trichostrongylus axei (3.95%), Mecistocirrus digitatus (2.63%), Trichostrongylus colubriformis (7.89%), Cooperia oncophora (9.21%), Cooperia punctata (5.26%), Cooperia pectinata (6.58%) , Nematodirus helvetianus (2.63%), Bunostomum phiebotomum (2.63%), Strongyloides papillosus (2.63%), Oesophagostornum radiatum (3.95%), Trichuris globulosa (1.32%), Paramphistomum cervi (3.95%), Paramphistomum microbothrium (2.63%) and Monezia benedeni (2.63%)
The effect of helminthiasis on blood parameters like, total erythrocytic count, total leukocytic count, packed cell volume, erythrocytic sedimentation rate, haemoglobin concentration was investigated and the values recorded were 5.840±0.110x106 Cimm, 5.447±0.074x103 Cumm, 32,858±0.230%, 38.004±0.217 mm/hr. and 10.368±0.096 gm/lOOml respectively. The differential leucocytic count values for neutrophils, lymphocytes, eosinophils, monocytes and basophils were
43.16±0.531%, 43.82±0.718%, 6.40±0.187%, 5.620±0.241% and 1.040±0.124% respectively.
Similarly the helminth free (control> animals studied for their total erythrocytic count, total leukocytic count, packed cell volume, erythrocytic sedimentation rate, haemoglobin concentration, gave their averages as 7.382±0.068x106 Cmm, 6.321±0.084x103 Cimm, 38.927±0.286%, 28.213±0.148 mm/hr. and 11.892±0.286 gm/lOOml respectively. The differential leukocytic count values for neutrophils, lymphocytes, eosinophils, monocytes and basophils were
29.15±0.365%, 56.0±0.513%, 8.12410.28%, 5. 852410 .264% and 0.90±0.191% respectively.
The average values for helminth infected and helminth free animals were analysed statistically. The infected animals had a significantly decreased total erythrocytic count, total leukocytic count, packed cell volume, haemoglobin concentration, lymphocytes and eosinophils with increased erythrocytic sedimentation rate and neutrophils, while no significant difference was seen in monocytes and basophils.
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34.
The Effects Of Induced Coccidiosis On Growth And Blood Parameters In Commercial Quails (Coturnix Coturnix
by Ashraf, M | Dr. Asif Rabbani | Dr. Mubashar Saeed | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: The study was designed to see the effects of experimentally induced coccidiosis op growth and blood parameters in commercial quails (coturnix coturnix japonica). For this purpose 200 day old quail chicks were obtained from a local hatchery and raised under standard, controlled coccidia free conditions. A commercial coccidiostat free feed was provided. At the age of 21 days, birds were divided into 2 groups A and B each comprising 100 birds.
Birds of group 'A' were kept as non infected control and birds of group 'B' were infected with I ml of coccidial inoculum having 50,000 sporulated oocysts given directly into crop. After the infection, the OPG of the both groups was recorded. Weekly weight gain/bird of both A and B group was recorded. Meanwhile the morbidity, mortality, clinical finding and post-mortem finding were also observed. At the end of experimental period of 42 day, 20 birds from each group were slaughtered and blood sample were collected for haematology.
The weight of birds in group 'B' was significantly lower than that of group 'A' birds. The morbidity and mortality rate in group B were 100% and 38% respectively.
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35.
A Study On Gastro Intestinal Nematodes Of Camels Slaughtered At Metropolitan Corporation Abattoir Lahore With Taxonomy Of The Isolated Species
by Abbas Ali, Syed | Dr. Asif Rabbani | Dr. Mubasher Saeed Mian | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: The present study was carried out to determine the incidence of gastro-intestinal nematodes of camels, with the taxonomy of the isolated species. Sixty guts were examined rendomly at the rate of 15 guts per month from August to November, 1993. The adult parasites were collected, fixed and preserved. The whole mounts were prepared. The faecal samples were examined and the EPG was estimated using McMaster egg counting technique. The taxonomy of the isolated species was determined by examing the whole mounts, according to the morphological characters given in the keys. The five species of nematodes representing different genera were isloated and identifed as Haenionchus ion gistipes, Izlaemonchus contortus, C'ainelostrongy!us mentulatus, Neniatodirus spathiger and Trichuris giobulosa.
The overall incidence recorded from camels during the present study was 65 percent. The morphological characters were studied by preparing permanent mounts and were described.
The findings of the present study will help in planning programme for the control of parasitic disease, especially caused by the above mentioned species of nematodes of camels in Pakistan, which will contribute to the development of animal industry by providing much effective coverage for the devastating effects of the parasites and will ultimately enchance the production of milk, meat and hides.
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36.
A Study On The Epidemiological Aspects Of Fascioliasis In Buffaloes In Lahore District
by Rabbia Sahar | Dr. Asif Rabbani | Dr. Haji Ahmed Hashmi | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1996Dissertation note: The study was designed to find the prevalence and intensity of liver fluke infection in buffaloes in Lahore District. The study period was from April 1995 to July 1995. The data was based on:
Data collected from four Veterinary Hospitals in Lahore District namely, Herbenspura, R.A. Bazar, Shamkey Bhattian and Rukh Chandra indicated that during the study period from 1 April, 1995 to 31 July, 1995, a total of 2184 buffaloes were treated for different diseases in these hospitals. Based on history, clinical symptoms and response to treatment 229 (10.48%) buffaloes were positive for fascioliasis.
Information collected from Municipal Corporation, Lahore abattoirs regarding economical losses revealed that one hundred eight million rupees were lost annually due to damage and condemnation of liver fluke infected livers.
Two hundred faecal samples collected directly from the rectum of buffaloes from various localities at Lahore were examined microscopically by fresh smear and sedimentation methods, which revealed that 75 (37.5%) buffaloes were suffering from fascioliasis.
In order to determine the intensity of Fascioliasis in infected animals, quantitative faecal examination was done (and it ranged from 32.6 to 45.6 EPG indicating that the disease is likely to be pathogenic).
Overall liver fluke infection was recorded by examining forty livers along with their bile ducts collected from slaughtered buffaloes and it was found that 16 (40%) had liver fluke infection.
Identification of live flukes from the infected flukes revealed that 8 (50%) had mixed infection of Fasciola hepatica and Fasciola gigantica, 5 (31.25%) had only Fasciola gigantica infection while 3 (18.75%) had only Fasciola hepatica infection.
It was concluded from the above studies that Fascioliasis in buffaloes in Lahore district is quite prevalent, and it needs to adopt appropriate measures to control the infection.
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37.
Taxonomical Study Of Treatment Infections And Their Effect On Blood Picture In Sheep And Goats
by Sulman Hameed | Dr. Asif Rabbani | Dr. Haji Ahmad Hashmi | Dr. M. Sarwar | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1996Dissertation note: In the present study two hundred sheep and two hundred goats both above one year of age and below one year age were examined after their slaughtering at abattoirs of Lahore for the presence of Gastrointestinal trernatodes during the month of May to August, 1995.
Monthwise prevalence of p. Cervi, f... hepatica a6% D. dendriticum and D. dendriticurn during the month of May to august, 1995, was as under:
May 1995 15% cervi, 20% f hepatica 6% D. dendriticum
June // 19% P.cervi 27% f. hepatica 8% D. dendriticum
July // 33% P.cervi 40% hepatica 16% D. dendriticum
August // 28%P.cervi 32% f hepatica 13% D. dendriticurn
It was observed that the infection increased gradually and reached at peak in the month of July and then declined. An overall incidence of 64.25% of trematodes was recorded in both the species. The parasitic incidence was as . cervi 23.75%, .J hepatica (29.75%) and .. dendriticum (1 0.75). So the rate of infection was on the peak in the month of July in P.cervi, E. hepatica and . dendriticurn as shown in Figure 1. The hernatological values of sheep and goats infected with trernatodes were :total Erythrocytic count 1 2.01 3 ± 0.096 million/cubic microliter, Haemoglobin level 8.037±0.160 g/l00rnl, Packed cell volume 30.10 ± 0.326%, IErythrocytic sedimentation rate 2.71 ± 0.242 mrn/24 hours and Total Erythrocytic count 10.973 ± 0.094 million/ microliter, Haemoglobin 7.637±0.128 g/1 OOml, Packed cell volume 28.15 ± 0.316% and Erythrocyte sedimentation rate 1.94±0.246 rnm/24 hour respectively for sheep and goats. It was observed that the infection with trernatode had an adverse effect on total erylhrocyte count, haemoglobin level and packed cell volume.
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38.
Studies On The Incidence Of Gastro Intestinal Helminths And Their Economic Impact On Production Potential Of Sheep
by Zia ur Rehman, Qazi | Dr. Asif Rabbani | Dr. Muhammad | Dr. Muhammad Afzal | Faculty of Veterinary Sciences.
Material type: ; Format:
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not fiction
Publisher: 1998Dissertation note: A study of gastro intestinal helminth parasites of sheep and goats of District Peshawar has been conducted, covering a period of 4 months (May to August, 1998). During this study incidence of GIT. helminths and their impact on production potential (weight gain) of sheep and goats was studied. Random collection of samples were made from Peshawar slaughter house and a total of 40 guts each in sheep and goats were examined and following species of helminths were recovered and identified. Oesophagostomum columbianum, 0. venulosum, Trichuris parvespiculum, Haemonchus contortus, Chabertia ovina, Bunostornum trigonocephalum, Nematodirus spathiger, Moniezia benedeni, Moniezia expansa, Trichuris lani, T. globulosa, Cysticercus tenuicollis.
Only two species of cestodes and nine species of nematodes were recorded during the study. However no trematode specie was recorded. Amongst the nematodes Trichiurisparuespiculum infection was recorded to be the highest in sheep and goats with an incidence of 64.70% and 73.33% respectively. The incidence of Haemonchus contortus was 24.19 and 53.33% in sheep and goats respectively. The worm burden ranged from 1-470 in sheep and 3-141 in goats respectively.
Out of total gastro intestinal tracts examined (40 each in sheep and goats), 73.33% and 60.29% were found positive for mixed infection in sheep and goats respectively.
In order to assess the effect of GIT helminths on live weight gain and its measurement in terms of economics of the above mentioned animals, 0 Hasthnagri sheep and 30 beetal goats were divided into two groups (control vs. treated by Nilzan Plus of ICI). The average daily weight gain in sheep group was 83.78 gms while in goats it. was 85.26 gms per animal. On comparison with treated group, the increase weight gain was 3.03 kg and 2.56 kg in sheep and goats respectively at the end of the experiment. Calculating the increase on live weight basis Rs.234.5 and 158 per animal were earned more in sheep and goats respectively. After deducting the cost of anthelmintic treatment the net gain Rs.222.5 and Rs.146 per animal in sheep and goats. During this study a progressive decrease in faecal egg counts with increasing age was observed both in sheep and goats.
Statistical analysis by using un-paired "t" test confirmed that there was significant differences in weight gain and faecal egg counts, between control and treated groups of sheep and goats.
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39.
Study On The Prevalence Of Genus Trypanosoma And Effects Of Natural Infection On Various Blood Parameters In Dogs
by Afzal, M | Dr. Kamran Ashraf | Dr. Asif Rabbani | Dr. M. sarwar | Faculty of Veterinary Sciences.
Material type: ; Format:
print
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not fiction
Publisher: 1998Dissertation note: The study was conducted to determine the prevalence of genus trypanosome and the effects of natural infection on some blood parameters in dogs.
For this purpose blood samples of 500 pet dogs (250 adult dogs and 250 pups) were examined at Dog Hospital, College of Veterinary Sciences, Lahore. Giemsa staining procedure was admpted to stain glass slides having blood smears made from blood samples.
Out of 500 ogs, 30 dog were found positive for tryponosomiasis. It revealed that only 6% prevalence of trypanosomiasis in dogs was estimated. In case of adult dogs out of 250 dogs, 13 dogs (5.2%) and in pups out of 250 dogs, 17 dog (6.2%) were found trypanosome infected. So pups were found more susceptible as compared to adult dogs. During research it was also noticed that mot of the dogs which were found positive being kept with horses.
Then blood samples of 10 adult dogs and pups each which wre positive for trypanosomiasis and 10 adult dogs and pups each which were healty (as control) were further examined for the estimation of haemoglobin paced cell volum, erythrocytes sedimentation rate and total serum protein to check the effects of natural infection on mentioned blood parameters.
The blood parameters of trypanosome infected and healthy adult dogs were compared and similarly blood parameters of trypanosome infected and healthy pups were compared. For this comparison unpaired 't' test was applied. By calculation it was found that haemoglobin, packed cell volume and total serum protein of trypanosome infected adult dogs and pups were significantly decreased as compared to healthy adult dogs and pups, respectively. But the erythrocytes sedimentation rate was found significlantly increased in case of trypanosome infected adult dogs and pups as compared to healty, adult dogs and pups.
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40.
A Study On The Effect Of Experimentally Induced Coccidiosis On Weight Gain And Haematological Parameters And Its Control In Qualis
by Abdul Razzaq | Dr. Asif Rabbani | Dr | Dr. Kamran Ashraf | Faculty of Veterinary Sciences.
Material type: ; Format:
print
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not fiction
Publisher: 1999Dissertation note: The present study was conducted to see the effects of the experimentally induced coccidiosis on weight gain and some blood parameters and its control with medication and vaccination. For this purpose, 150 day-old quail chicks were purchased from the local hatchery and were reared under control managemental and coccidiosis free condition upto the 42 days of experiment. A commercial coccidiostat free feed and drinking water were provided ci libitum throughout the experiment. The birds were divided in five separate groups i.e. A, B, C, D and E, each comprising of 30 birds. Group A was kept as a non-infected, non-medicated and non- vaccinated. The chicks of groups B, C, D and E, were administered 50,000 oocysts of. tenella directly into the crop at the 18 days of age, the chicks in group C were immunized against coccidiosis with a locally prepared vaccine on 3rd and 10th day of age, while those of group D with an imported coccidiosis vaccine (Coccivac) on 7th day of age. The chicks of group E were medicated with Salinomycin on the appearance of clinical symptoms and OPG of the infected birds.
No oocysts were seen in the droppings of group A chicks, throughout the experiment, while group B, chicks had the highest OPG count after induction of infection, the highest peak being in the 3rd week post-infection.
The highest mortality recorded in the infected B group and low in other infected C, D and E groups and highest mortality in 3rd week of induced infection in all infected groups.
The birds in groups A and E attained highest weight gain as compared with groups B, C and D but the difference was non significant.
The lowest Hb and TEC values were recorded in infected group B, while in other infected groups C, D and E both these values were also decreased but comparatively lesser than B group. Similarly, values of TLC were increased in infected group B as compared with other infected groups i.e. C, D and E and the difference was statistically significant.
In case of differential leukocyte counts there were increased heterophil and monocytes values in group B, while decreased values lymphocytes, basophils and eosinophils were recorded in other infected groups than the other i.e. C, D and E.
The postmortem lesions observed were swollen intestines petechial haemorrhages on the wall of the intestine and dehydrated liver and lungs in infected carcasses.
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41.
Taxonomy & Chemotherapeutic Control Of Prevalent Helminth Species In Indigenous Poultry
by Abid Farid | Dr.Kamran Ashraf | Dr. Asif Rabbani | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2000Dissertation note: The present study was conducted to identify the helminth infestations, compare the drug efficacy percentage of I vermectin and Albandazole, effect of chemotherapy on haematology in indigenous poultry.
For this purpose, one hundred and sixty (160) positive birds were purchased from market and backyard farms, divided in four groups A, il, C, and D, kept in experimental rooms of College of Veterinary Sciences, Lahore.
Eggs per gram (EPG) of faeces of all the four groups was done at zero day of the experiment which were, 2307, 2000, 2050, 2325, in groups A , B, C, D, respectively. Groups A, Band C, were treated with Ivermectin and Albandazole (using 1% Ivomec Injection and Farbenda 10% solution).
After medication EPG was carried out of all four groups (A, D, C, D) at different days of experiment. The results were as, the EPG at 7th day 825, 667, 700, 2750, at 14th day 100, 250, 325, 3000, on
21st day. It was 70, 155, 237, 3500, and at 28th day the EPG. recorded was 45, 120, 185, 3600 respectively.
At end of the experiment, the drug efficacies were recorded, for group A, "it was 98%. In group B,94%, and for group C, it was 90% while group D, was untreated control.
The taxonomy of the slaughtered birds of group D, was done at the end of the experiment. By collecting GIT, and trachea of all the forty birds of the group D. Identifications were made after making permanent mounts of recovered helminths. Four species of nematodes were recovered from indigenous poul try. Ascaridia galli was the most common species of nematodes recovered from small intestine, while Heterikis gallinae and Subulura brumpti were isolated from the caeca of the birds. Syngamus trachea was the only respiratory tract nematode species which was recovered from trachea of the fowls. The incidence of Ascaridia gglli, Heterikis lZallinae, Subulura brumpti, Syngamus trachea were 57.5, 40, 20 and 2.5 percent respectively.
Six species of Cestode parasites were isolated from the small intesliine of Desi fowl, Raillictina tetragona, Raillictinna costcillus, Raillietina echinobothrida, Cotugnia dignopora, Choanotaenia infundibulum, Amoebotaenia sphenoides and the incidence recorded, 5235, 37.5, 20, 27.5, 22.5 and 2.5 percent, respectively
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42.
Epidemiological Studies On The Prevalence Of Coccidiosis In Commercial Broilers And Its Relationship With Other Diseases
by Umber Rauf | Dr . Asif Rabbani | Dr . Khalid Saeed | Dr. Shakil | Faculty of Veterinary Sciences.
Material type: ; Format:
print
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not fiction
Publisher: 1999Dissertation note: In this epidemiological study 26.48% birds of 26.82% farms were found affected with coccidiosis. Six species of Eimeria, E. acervulina. E. mitis, E. tenella, E. necatrix, E. praecox and E. mivati were observed. Number of oocysts in litter varied with the age of birds. Concurrent infections were detected in 80.68% cases, which included infectious coryza 21.95%, aflatoxicosis 27.45%, infectious bursal disease 16.94%, chronic respiratory disease 12.54%, Newcastle disease 10.16%, hydropericardium syndrome 9.83%, Escherichia coli 9.15% and Salmonella 8.13% infections. In the month of July and August , incidence of coccidiosis was high. Maximum incidence of disease was observed from 4th to 6th week while no disease incidence was noted during 1st and 8th week. It was also noted that the disease had drastic effects on Newcastle disease antibody titer.
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43.
Estimation Of Blood Haemoglobin, Serum Iron And Magnesium Level In Clinical Cases Of Ovine Haemonchosis
by Haneef ur Rehman | Dr . Asif Rabbani | Dr . Mubashar Saeed Mian | Dr . Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
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not fiction
Publisher: 1985Dissertation note: Haemonchosis is a serious helminthic infection of sheep and goats resulting in the mortality due to excessive blood loss and causes great economic losses. The objective of the present study was to determine the effect of natural infection of Haemonchus contortus in sheep upon blood haemo- globin, serum iron and magnesium level so that it could aid in accurate diagnosis and effective therapeutic measures. Infection was confirmed through faecal examination. Eggs per gram of faeces were calculated by McMaster egg counting technique. To see the effect of severity of infection on these blood components, blood samples of positive cases were taken from jugular vein.
Blood haemoglobin, serum iron and magnesium were estimated by using standard diagnostic kits with spectrophotometer. A decreased level of haemoglobin and serum iron was directly proportional to the severity of infection. However, no alteration in serum magnesium level was observed in infected and non-infected animals.
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44.
Toxonomical Study Of The Genus Trichuris And Comparative Anthelminitc Efficacy Of Fenbendazole (Panacur) Ad Albendazole (Valbazen) Against Gastro-Intestinal Nematodes in Sheep
by Dr. Muhammad Iftikhar | Mr. Asif Rabbani | Mr. M. Akram | Mr. Muhammad Afzal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1990Dissertation note: Pakistan has a large population of livestock, which plays an important role in the economy of the country. Parasitism is a major problem in our country. Great economic losses have been attributed to the sheep population in our country due to the parasitic infections. The genus Trichuris includes some of the most important nematode species effecting the sheep, therefore a taxonomical study of the genus Trichuris was conducted in the month of September and October, 1989. For this purpose 100 guts (large intestine) of sheep slaughtered at Lahore abattoir were procured and brought to the parasitology laboratory College of Veterinary Sciences, for further investigations. The overall incidence of Trichuris spp. was found to be 74% . The following two species were recorded.
1. Trichuris ovis 66%
2. Trichuris globulosa 18%
Taxonomy of Trichuris species was studied by preparing permanent mounts. The present work was also aimed to see the effect of two commonly available anthelmintics namely fenbendazole (panacur) and albendazole (valbazen), on naturally infected sheep with gastrointestinal nematodes. Sixty sheep positive for nematode infections were randomly divided into three groups A,B & C each group comprising of 20 animals. Group A and B
were given fenbendazole and albendazole respectively according to the manufacturers dose rate while group C was kept as untreated control. The efficacy of both the drugs was evaluated on the bases of reduction of eggs per gram of faeces after medication. The egg counts were made on zero, 7th, 14th and 21st day. Both the drugs revealed a progressive decrease in the faecal egg counts. The efficacy of fenbendazole on 7th, .14th and 21st day was 96.33, 97.05 and 99.66% respectively while the efficacy of albendazole was 92.56, 95.37 and 98.18% respectively. There was no significant difference in the effectiveness of the drugs.
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