Study Of Enzyme Leakage At Various Egg-Yolk Levels During Freezing Process In Nili-Ravi Buffalo Semen
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Publisher: 1993 Dissertation note: Present study was carried out on semen of 10 bulls over a period of 3 months maintained at Semen Production Unit, Qadirabad District Sahiwal. Semen sample$were evaluated for volume, mass motility, initial motility and sperm concentration. Accepted ejaculates having more than 60% initial motility were used for GOT, GPT and Hyaluronidase activity estimation. The Acrosin activity could not be estimated in diluents by the fact that optical density increases as a result of hydrolysis of substrate.
The GOT. GPT & Hyaluronidase values recorded in fresh semen were 38.27, 7.15 & 3.6 mu/ml, respectively. After extension of semen in lactose fructose egg yolk glycerol extender with five egg yolk levels i.e. 10, 15, 20, 25 & 30%, the GOT activity with these dilutions was found 65.74, 58.06, 56.08, 54.06 & 53.98. Whereas, these values were 18.74, 11.97, 10.84, 10.25 & 9.95 units for GPT. Under the same dilutions the Hyaluronidase activity was estimated as 6.71, 5.00, 4.91, 4.90 & 4.89 mu/ml, respectively.
After freezing, the GOT was found to be 65.78, 62.55, 61.40, 60.40 & 59.60 mu/ml for the same extended samples. The GPT values were 24.37. 18.03, 16.17, 16.01 & 15.93 mu/ml, and the recordes for hyalyronidase activity were 9.00, 8.00, 7.75, 7.79 & 7.78 mu/ml for 10, 15, 20, 25 & 30% egg yolk levels, respectively.
In vitro the most reliable & preferable method considered for the evaluation of semen preservation technique is the analysis of seminal plasma for several enzymes related with fertility, because the extra cellular concentration of these enzymes might serve as an index of membrane trauma.
In the light of results obtained in the present study and the values recorded at different egg yolk levels for the estimation of GOT, GPT and hyaluronidase enzyme activity indicated that 20-30 % level was comparatively more superior for the extension and deep freezing of buffalo semen.
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