1.
The In Vitro Study On Comparative Digestibility In Cow And Buffalo Of Different Fodders Availale In West
by Mohsin Shah, S.M | Dr.Muhammad Yaqub Malik | Not Available.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1970Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0065,T] (1).
2.
Effect Of Two Anticoccidials With And Without Supplementation Of Vitamins On Some Liver And Kidney Functions
by Muti Ur Rehman Khan | Dr. Shakil Akhtar Khan | Dr.Javed Rashid | Dr.Muhammad Amin Sheikh | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1998Dissertation note: The project was designed to study the effect of anticoccidials (Ampisol 20% and Esb3 30%) with and without supplementation of vitamins on some liver and kidney functions of broiler chicken. A total of hundred day old broiler chicks were randomly divided into five equal groups designated as A, B, C, D and E. Group A was medicated with Ampisol 20% at the dose rate of 3 gm/10 liters. Group B was given Ampisol 20% alongwith supplementation of Symodek at the dose rate of 1 gmJ2 liters. Group C was medicated with Esb3 30% while group D was Esb3 30% plus Symodek. Group E was non- medicated control. Serum samples were collected randomly from five birds in each group on day 21, 28, 35 and 42 and were stored at -20°C uptil tests. The experimental period was 42 days. Estimation of alanine aminotransferase, aspartate aminotransferase, serum creatinine and serum uric acid was made by using commercially available kits in collected serum samples of all groups.
The statistical analysis of alanine aminotransferase, aspartate aminotransferase, serum creatinine showed no significant difference among groups A, B, C, D and Eon 21, 28,35,42 day of experiment. Serum uric acid levels showed no significant difference in different groups on 21 and 42 day. On day 28 of the experiment there was no significant difference between mean serum uric acid levels of group C and D but were significantly different from groups A, B and E. The mean uric acid levels of groups A, B and E were significantly different from each other. On day 35 there was significant difference among different groups except between B and C groups. As there were no adverse effects on liver and kidney functions of broiler chickens, the administration of Ampisol 20% and Esb3 30% with and without vitamins is recommended for chemoprophylaxis of coccidiosis in broiler chickens.
Availability: Items available for loan: UVAS Library [Call number: 0576,T] (1).
3.
Effect Of Replacement Of Maize With The Varying Levels Of Stabilized Extruded Rice Polishing On The Performance Of Broilers
by Aqif Mukhtar, Syed | Prof.Dr. Nisar Ahmad Mian | Dr.Anjum Khalique | Dr.Muhammad.
Material type: ; Format:
print
Publisher: 1998Dissertation note: As the poultry industry flourished rapidly during the last thirty years, it has now become a major contributor in providing low cost protein of animal origin to our population. But unfortunately poultry industry is continuously suffering loses for the past few years mainly due the fact that the conventional feed stuffs are becoming costly day by day. The serious food shortage and increasing competition between human beings and poultry regarding the consumption of cereal grains demanded that cereals should be now spared for human consumption and exploration of non conventional feed sources should be carried out in order to keep the business of poultry industry running well.
Rice polishing is a by-product of rice milling so it is abundantly available in Pakistan because rice is the third largest crop produced in the country. Rice polishing compares well with the cereal grains in chemical composition. So it contains more fat and protein as compared to maize but high in fiber contents. Due to high fat contents rancidity of Rice polishing can pose some problems if it is fed after prolonged storage, specially in hot weather.
Extrusion cooking is a new technique which stabilizes the high fat diets and make them fit for use in the animal rations. For this reason full fat rice polishing was subjected to Extrusion Cooking which was done at National Feed Mill.
Present Experiment was conducted to replace maize with extruded rice polishing and to see the effects on the performance of broiler chicks. Six starter and six finisher rations were prepared and designated as A, B, C, D, E and F . All rations were isocaloric and isonitrogenous. Starter ration and finisher ration A. were kept as a control containing 35 % Maize and 0% extruded Rice polishing, which was replaced by extruded rice polishing in ration B, C, D, E and F. on protein equivalent basis at 15, 20, 25, 30 and 35% level In this way a complete 100% replacement of maize with extruded Rice polishing was made One hundred and eighty day old chicks were randomly divided into eighteen experimental units of 10 chicks each. Each experimental unit was placed individually. The starter rations were fed up to 28th day of age while the Finisher rations from 29th to 42nd day of age.
Results of the present study indicated that the weight gain up to 25% extruded rice polishing level was promisingly good. Maximum weight gain was recorded in Ration B (15% extruded rice polishing). Second best results were noted in the control ration but the performance of the chicks fed on 20% extruded rice polishing was almost equal to that of control ration. It was noted that the weight gain started to decrease gradually as the level was raised up to 35% of the total with each increasing increment.
Best feed utilization was observed in the chicks fed on ration B, whereas poor utilization of feed was noted in higher levels of extruded rice polishing in the diet having more feed consumption compared to weight gain.
Better performance up to 25% level indicated that Extrusion improved the nutritional quality of rice polishing by stabilizing it and inactivating Lipases. The extrusion also made starch more available for the birds and reduced the anti-nutritional elements of rice polishing. This is evident from the fact that the level of rice polishing effected the health of the chicks.
It was therefore concluded from the present study that extrusion is an excellent technique for converting low quality feed stuffs into feeds with higher nutritional quality. Specially extrusion of low priced feed ingredients such as rice polishing and it replacement with a costly feed ingredient such as wheat or maize holds good economic prospects for feed millers. As extrusion is done on a very limited scale therefore after the present study it was strongly recommended that the use of extruded feed stuffs should be promoted in poultry ration
Availability: Items available for loan: UVAS Library [Call number: 0583,T] (1).
4.
Post Vaccinal, Observation In Lymphoidal, Organs (Bursa, Spleen, Thymus) Of Broiler Chicks Inoculated
by Shajeela Irum | Dr.Sameera Akhtar | Dr.Muhammad Amin Sheikh | Dr.Shakil | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1999Dissertation note: Infectious bursal disease (IBD) is a viral infection of chickens, causing degeneration of bursa of Fabricius and producing suppression in humoral immune response. Different vaccines are available in the market for mass scale immunization of chickens. Some contain more virulent and invasive strains than the others. Since the primary site of infection and inducement of lesions by IBDV is the hursa of Fabricius, the effect on the immune system may be significantly suppressive. This study compared two intermediate (228-E and BUR 706) and a mild (Gumborol CT) vaccinal strains of IBDV in terms of their ability to induce an antibody response and to cause damage to different lymphoid organs in chickens.
A total of 250 chicks (divided into 4 groups) were vaccinated with different strains of IBDV and the antibody levels were monitored using indirect haemagglutination (IHA) test every week post-vaccination upto 5 weeks. IHA revealed that the vaccinated with 228-E or BUR-706 had significantly higher antibody titers (GMT 8.0, 7.7, respectively) as compared to Gumborol CT vaccinated birds (GMT 3.0) on 35 days post-inoculation, On day 25 post-vaccination, some birds from each group were challenged with a fully virulent field strain of IBDV, to study whether the antibody levels were protective than the unvaccinated ones. Furthermore intermediate strains were found to be more damaging to the bursae and spleens than the milder one since lower bursal and splenic body weight ratios were recorded in them.
The study suggested the use of intermediate strains a vaccine since they induced high antibody titers as compared to that of the milder strain. However, more invasive and pathogenic intermediate strains used in this study caused more damage to the lymphoid organs harbouring B cells. So the need exists for an effective infectious bursal disease vaccine, low in virulence, which could be applied by a mass vaccination in chickens conferring excellent protection against the disease with minimum immunosuppressive effects.
Availability: Items available for loan: UVAS Library [Call number: 0606,T] (1).
5.
Comparative Efficacy Of Different General Anaesthetics With & Without Surgical Intervention In Dogs
by Faisal Ibrahim Khan | Dr.Muhammad Arif Khan | Dr.Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1999Dissertation note: These days the scope of anaesthesia is consistently increasing due to increase in surgical patients" in veterinaty practice. This change in trend is. due to the awareness of the owners to different surgical problems and their solutions. They do "not hesitate to get their animals treated with modem equipments and highly expensive medicine. To meet this challenge and demand of the owners, veterinary surgeons are taking keen interest to get the advanced knowledge and tatest information of modem equipments and their usage in veterinary practice.
In the past surgery was perfonned by making tempormy insensibility by asphyxiation, cerebral concussion by striking wooden bowl placed.over the head with a hammer and regional aneathesia was attained by compression of nerves and blood vessels of the region to he operated upon. But these days different sedative and anaesthetic agents are being used to make the animal desensitize from all extemal stimuli. These drugs are available in volatile, nonvolatile and gaseous forms. The usage of anaesthetic agent entirely depends upon the species, age, weight, temperament of the patient, nature of the operation to be perf01med, cost and availability of the drug.
In this project tlu°ee different general anaesthetics were tried in 18 animals of canine species to find out the best selection of anaesthesia with and without surgical interventions. The animals were divided into three major groups of six. animals each. These groups were designed as A, Band C. The major gronps were further divided into sub groups At, A2, Bl, B2, Cl and C2 respectively. Sub group was anaesthetized with pentothal sodium without surgery, sub groups B1 with ketamine and sub groups C1 with halothane gas anaesthesia. In sub groups A2, B2 and C2 surgical operations castration was performed with their respective general anaesthetics. The efficacy of these general anaesthetics was measured on the bases of nature of induction, recumbency period, nature of recovery, temperature, pulse TLC, TEC, PVC, Hb, ALT AST, Serum Creatinine, Serum Urea and Statistical analysis of results.
The analysis of results collected during the study clearly indicated that halothane gas anaesthesia was a better selection for anaesthetizing canine patients for minor and major surgeries.
It was concluded that halothane gas anaesthesia should he used for all types of major surgical procedures in canine practice.
Availability: Items available for loan: UVAS Library [Call number: 0617,T] (1).
6.
Comparative Nutritional Evaluation Of Poultry By Product Meals Prepared By Two Separate Processes In Broilers
by Aftab Ahmad | Prof.Dr.Talat Naseer Pasha | Dr.Muhammad | Mr.Anjum Khalique.
Material type: ; Format:
print
Publisher: 2000Dissertation note: Protein as a second major factor in formulatiQn of poultry rations after energy. It is supplied by animal and vegetable sources. Animal protein supplement are superior in quality due to its more balanced amino acid profile but are costly. While vegetable proteins are cheaper but lacking in some amino acid and have some antiS nutritional factor. The animal protein supplements available in Pakistan are blood, fish, meat and PBPM. PBPM is abundantly available in Pakistan and it is prepared by using chicken feet, heads, viscera, feather skin and blood. And processing varies from organization to organization. PBPM is prepared by different organization such as M. Artal, BeBeJan and Kakasian.
PBPM has low cost and its CP and ME are comparable to fish meal, but it is low in lysine and methionine. Our present study was to compare the effect of processing of PBPM manufactured by BeBeJan and Kakasian feed industry. Both have different composition and different processing method of PBPM as blood was included in BeBeJan PBPM but it was absent in Kakasian.
The objective of study was to evaluate nutritional profile and quality of commercially available PBPM, the effect of processing on performance of broiler chicks. 240 day old Hubbard chicks were purchased from the local marked and were divided into 8 groups from A to H which were again subdivided into 3 replicates of 10 birds each. Eight experimental starter and 8 experimental finisher rations of A, B, C, 13 and E, F, G, H were prepared. A and E were the control group while B, C and D having 3, 6, 9% PBPM of BeBeJan and F, G, H having 3, 6, 9% PBPM of Kakasian were formulated and starter rations were fed to chicks upto 28 days of age while finisher was provided from 29 to 42 day of age. The feed was supplied to the chicks reared in cages at College of Veterinary Sciences, Lahore. The proximate analysis of the rations was done. And the parameters studied were weekly weight gain, feed consumption, FCR. At the end of the experiment three birds from each group were slaughtered to observe the dressing percentage and weight of internal organs. The economics of the experiment was also calculated. The pepsin digestability of both the organizations PBPM was also recorded. The data thus collected was subjected to statistical analysis. The results indicated that in BeBeJan group there was a significant weight gain in control group A (O%PBPM) while in Kakasian it was significant in F group having 3% PBPM. While comparing both organization PBPM it was observed that F&H rations having Kakasian PBPM 3% and 9% were better than B and D ration having BeBeJan PBPM 3 and 9% regarding weight gain while FOR of BeBeJan it was better in control group A (0% PBPM) while in Kakasian it was better in F group having 3% PBPM. While comparing both organations it was observed that Kakasian PBPM showed better FCR than that of BeBeJan. And it might be due to processing and no adultration of blood in PBPM by Kakasian, while BeBeJan PBPM contained blood. The dressing percentage of control group was better than treated group while comapring both organizations PBPM was non-significant difference among the groups.
Availability: Items available for loan: UVAS Library [Call number: 0632,T] (1).
7.
A Study On Bacteriological Examination Of Raw And Pasteurized Milk Marketed In Lahore Area With Particular Reference To Public Health
by Aman Ullan | Dr.Muhammad Akram Munir | Dr.Khalid Saeed | Dr.Masood Rubani | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Literary form:
not fiction
Publisher: 2001Dissertation note: The present study was conducted to study the bacteriological quality of various types of milk being marketed in Lahore. A total of 120 milk samples consisting of equal number (30 each of raw, pasteurized, UHT and sterilized milk) from various sources were examined for standard plate counts using milk agar, coliform count using EMB agar and using milk ring test (MRT) for brucellosis.
All the samples were negative for MRT. Average standard plate count (SPC) and coliform counts for raw milk were 2.34x107 cfulml and 2.2x105 cfu/ml respectively. Similarly, SPC and coliform counts for pasteurized milk were found as 2.16x105 cfuJml and 3.28x104 cfu/ml respectively. No raw milk sample fulfilled the International Standards for very good quality milk for SPC or coliform count. For pasteurized milk, only 33.33% samples fulfilled the International Standards for very good pasteurized milk. SPC and coliform counts for UHT or sterilized milk was zero thus fulfilling the International Standards.
A total of 162 isolates (88 isolates from raw milk and 74 isolates from the pasteurized milk) were identified. These isolates included Staphylococcus epidermidis, 38 strain (21 from raw milk and 17 from pasteurized milk), Staph. aureus, 30 strains (16 from raw and 14 from pasteurized milk), Escherichia coli, 24 stains (14 and 10), Enterobacter aerogenes, 20 (11, 09), Bacillus cereus, 22 (15, 07), Micrococcus luteus 15, (03, 12) and Klebsiellapneumoniae 13 (08, 05) strains. vitro antibiotic sensitivity tests indicated that all the isolates were resistant to penicillin whereas most of the isolates were sensitive to Gentamicin, Kanamycin and Chloramphenicol. Escherichia coli was resistant to most of the commonly used antibiotics.
Overall hygienic quality of raw and pasteurized milk available in Lahore city was graded as poor.
Availability: Items available for loan: UVAS Library [Call number: 0802,T] (1).
8.
Studies On The Effect Of Ammoniation On The Nutritive Value Of Different Roughages In The Fattening Of Lambs
by Talat Naseer Pasha | Dr.Muhammad Yaqoob Malik | Dr . Manzoor | Dr.Saghir Ahmad Jafri.
Material type: ; Format:
print
Publisher: 1984Dissertation note: Sufficient quantity of agricultural by-products in the form of dry roughages are available in the Country for livestock feeding. The nutritive improvement of these roughages could be accomplished by proper chemical treatments. Ammoniation is one of the methods employed to improve the nutritive value of dry roughages for feeding of livestock.
A fattening trial was conducted on four groups of 64 male of Lohi lambs breed to study the effect of ammoniatin on the nutritive value of wheat straw and sugarcane pith. The roughages were treated with ammonia at 3.5 % level for 15 days and used at 22.5 % level of the four experimental rations having untreated and ammonia treated wheat straw and sugarcane pith. The experiment lasted for 84 days. Feed consumption, weight gain, feed efficiency and digestibility of the animals were recorded.
There was a significant (PLO.05) improvement in the weight gain and feed efficiency of the lambs fed ammonia treated roughages as compared with the non-treated roughages. However, there was no significant difference in the feed consumption of the animals fed different experimental rations. The digestibility trial revealed that the differences with regard to the digestibility of dry matter, crude protein,crude fibre, ether extract and nitrogen free extract between treated and untreated sugarcane pith and wheat straw were F; non-significant. But when both the roughages whether ammonia treated or untreated were compared with each other, wheat straw had significantly higher values of nutrients digestibility as compared with sugarcane pith.
The study indicated that ammoniation of wheat straw and sugarcane pith improve their nutritive value at a significant level (PLO.05) for the fattening of lambs.
Availability: Items available for loan: UVAS Library [Call number: 0804,T] (1).
9.
Prevalance And Anthelmentic Activity Of Indigenous Plants Against Trichostrongylus Of Sheep In District Zhob
by Nasib Ullah | Dr.Muhammad Lateef | Prof.Dr. Azhar Maqbool | Prof.Dr.Zafar | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2010Dissertation note: A Trichostrongylid is gastrointestinal nematode which causes the heavy economic losses to the livestock particularly sheep. A total 240 gastrointestinal tracts of sheep from district Zhob abattoir were collected. These samples were isolated and identified for trichostrongylid nematodes. The overall prevalence was 50%(120/240), of which 39.1% in male and 60.8% in female sheep were recorded at slaughter-house. Plants were collected from area of district Zhob. These plants were Identified and authenticated by botanist .The crude aqueous methanolic extract of the plants were used for in-vivo studies. Eighty sheep of either sex, aged between three to six months and naturally infested with Trichostrongylid nematodes (including trichostrongylius spp. I-Iaemonchus contortus, cooperia etc) were selected and managed separately for the experiment. These sheep were divided into 4 groups A ,B,C and D. Group A was contained 10 sheep and was untreated and considered as control. Thirty (30) were kept in Group B, were further sub divided into three equal groups i.e. BI, B2 and B3 and treated with different levels of Chenopodium album @ 1, 2 and 3 g/kg body weight respectively. Group C having thirty (30) was also sub divided into three equal groups i.e. Cl, C2 and C3 and treated with different levels of Artemisia brevifolia @ 1, 2 and 3g/kg body weight respectively. Group D was treated with Levamisole @ 7.5 mg/kg body weight. Faecal egg count reduction was criterion for evaluation. Faecal samples were collected before treatment on day 0 and on day 3, 5, 7, 10 and 14 post treatments for EPG count.
The results of EPG for animals in group B1 at day 0 was 1325. This rate was reduced to 1250, 1125, 995 and 702 at day 3, 7, 10 and 14, respectively. Similarly EPG of sheep in B2 were 1280 at day 0 and reduced to 1205, 1202, 1001 and finally to 690 at day 3, 7, 10 and 14 respectively. Best results in B group against the nematodes were in B3 in which Chenopodium album was used 3g/kg bw. The results were 1250, 1231, 1145, 590 EPG at day 0, 3, 7, 10 and 14. The sheep in group Cl showed 1203 EPG at day 0, when treated with Ig/kg bwArtemisia brevifolia, the EPG was reduced to 1173, 1115, 700 and 528 on day 3, 7, 10 and 14 respectively. Second level of treatment C2 of
Artemisia brevifolia which was given @ 2g/kg bw initially contained reduced to 1202 EPG, on day 3, 7, 10 and 14 the EPG counts were 1020, 631, 546 and 459, respectively.
Highest dose of Artemisia brevfolia was 3g/kg body weight to sheep in group C3. On day zero the EPG count was 1196. On day 3, 7, 10 and 14th day the EPG count decreased to 1079, 905, 528 and 396 respectively. The sheep in group D, treated with recommended dose of Levamisole showed 1138 EPG prior to medication, which reduced remarkably to 681, 536, 357 and 147 on day 3, 7, 10 and 14 respectively. Although no untoward effects of plants were observed but best EPG reduction results (87.08 %) were observed in Levamisole as compared to treatment of 3gm/kg b.w Chenopodium album (51 .03 %) and Artemisia hrevfolia 3g/kgbw (66.88 %).
CONCLUSIONS
A wide variety of plants are naturally available in the Indo-Pakistan subcontinent which possess narrow or broad spectrum anthelmintic activities. No doubt this is true in other regions of the world as well where gastrointestinal parasitism is an important problem in livestock keeping, and the availability of commercial drugs may be limited. Conventionally, trichostrongylids has been tackled with use of synthetic anthelmintic but owning to development of anthelmintic resistance against major groups of anthelmintics viz., benzimidazole, Levamisole and avermectins, people are looking for alternatives to synthetic chemicals.
The phytochemical analysis of these plants and controlled anthelmintic trials along with contemporary knowledge of parasite control strategies may offer new opportunities for effective and economical control of parasitic diseases. So these plants can be better alternative for synthetic chemicals.
Quality control extracts of Artemisia brevifolia and Chenopodium album or possibly isolated bioactive compounds could be a promising alternative to conventional anthelmintics fbr the treatment of gastrointestinal trichostrongylids of small ruminants in the future. Such a treatment could be used in control strategies against gastro intestinal nernatodes in organic and conventional production systems. Further research is needed for studies on the bio active constituents, as well as on the reproducibility, dosage, application regime. toxicity and effectiveness of Artemisia brevifolia and C'henopodium a/bums in other host species and against other economically important gastro intestinal nematodes species.
RECOMMENDATIONS
It is recommended that further research could be carried out on large sample size in different seasons of the year and large number of plants, identification of active principles of plants with proven anthelmintic activity, standardization of dose and toxicity studies for drug development. In addition to this, large number of samples of the same plant from different geographic areas should be subjected to experimentation keeping in view the possibility of differences in chemical composition of soils.
Availability: Items available for loan: UVAS Library [Call number: 1075,T] (1).
10.
Designing A Lead (Drug)Molecule To Block The Dna Binding Site Of Cancer-Causing E2F3 Transcription Factor
by Muzammal Hussain | Dr.Aqeel Javeed | Dr.Muhammad | Prof.Dr.Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2009Dissertation note: As transcriptional factors are the current area of concern in novel anticancer drug designing, this study was designed to develop a suitable drug (lead) molecule to block the DNA-binding site of cancer-causing E2F3 transcription factor (overexpressed in prostate, lung, bladder cancers) by using computer-aided drug design approach and implementing homology modeling, molecular docking and virtual high-throughput screening techniques. A reasonable 3-dimensional structure model of E2F3 transcription factor was generated by following homology modeling technique and using SWISS-MODEL server. The stereochemical evaluation of the generated model was carried out by using the program PROCHECK. The active site residues of the DNA-binding domain that make critical contacts within the major groove of DNA were determined by analyzing the crystal structure of the template (E2F4). Then, by using this structure model a chemical database (containing 3D structures of available chemical compounds) ZINC was virtually screened: only those molecules having molecular weight between 300 to 350, neutral charge, hydrogen bond donors 0/1, hydrogen bond acceptors 3/5, rotatable bonds 2/7 and a value of xLogP between -2 and 4, were taken into account. The compounds yielded by this database filtration step were then subjected to 10 run docking studies with the program AutoDock 4.02 to search for the suitable hits. This step resulted in 31 hits. From
these hits the compounds with binding energy lower than -3.5 Kcal/mol and showing maximum hydrogen bonding interaction with active site were further selected. This step returned 6 compounds which were further evaluated by giving 30 runs of docking in the sense to improve the interaction with the active site residues (hydrogen bonding) and binding energy. 3 compounds with binding energy less than - 4.0 Kcal/mol were further subjected to visual inspection in order to evaluate their binding poses at the active site. One was eliminated and the remaining two were further subject to 50 docking runs see any improvement in ener4gy. One of the them showed a little improvement in biding energy, however, both were suggested as suitable ,leads, as the difference in their binding energies was very small and both were making equal number of hydrogen bonds with the DNA binding site of target F2F3
Availability: Items available for loan: UVAS Library [Call number: 1100,T] (1).
11.
Chemical Equivalence Of Different Brands Of Amoxicillin Trihydrate And Its Minimum Inhibitory Concentration
by Rana Adnan Ali | Prof.Dr.Muhammad Ashraf | Dr aftab Ahmad | Dr.Muhammad Adil Rasheed.
Material type: ; Format:
print
Publisher: 2011Dissertation note: This project was designed to study the chemical equivalence of different brands of amoxicillin trihydrate (long acting and short acting) approved by the ministry of health and available in the market for veterinary use. Amoxicillin was measured by HPLC method developed and standardized in the laboratory. Limit of detection (LOD) and limit of quantification (LOQ) of the amoxicillin trihydrate was determined. Solutions of different concentrations were prepared from amoxicillin trihydrate reference standard for the determination of LOD. and were protected from light and stored at 2-8 oC until used. The LOD calculated by us was 0.100 (µg / ml) and LOQ was 0.5 (µg / ml). Correlation Coefficient should be ? 0.99 and the result obtained by the data was 0.99984050. Chemical equivalence of all brands was determined by using HPLC systems (Shimadzu & Agilent). Concentrations for reference standard (50, 25 and 10 ?g /ml ) and for each brand (Alomox LA, Amovet LA, Farmox LA, Novamox LA, Trioxyl LA, Amoxi-vet, Colimox, and Colimoxin) were used. All the results obtained showed that maximum percentage of assay obtained among long acting was of the brand Farmox LA (101 %) and in case of short acting was of Amoxi-vet (101%). Minimum percentage of assay among long acting was of brand Amovet LA (92 %) and in case of short acting was of Colimox (96%). MIC of amoxicillin against E.coli and Staphylococcus was determined by micro broth dilution test. According to our results 73.33 % E.coli were susceptible and 26.67% were resistant to the amoxicillin trihydrate. Our results showed that 86.67% Staphylococcus were susceptible and 13.33% were resistant to Amoxicillin Trihydrate (Reference Standard). It showed that this antibiotic is still very effective against the diseases produced by the Escherichia.coli and Staphylococcus aureus.
Availability: Items available for loan: UVAS Library [Call number: 1249,T] (1).
12.
Prevalence And Biochemical Studies On Cattle Suffering From Babesiosis In District Swabi,Khyber Pukhtoonkha
by Naveed Khan | Muhammad Yasin Tipu | Dr.Habib-ur-Rehman | Dr.Muhammad Younas Rana.
Material type: ; Format:
print
Publisher: 2011Dissertation note: The present study was designed to diagnose Babesiosis in cattle in District Swabi Khyber Pukhtoonkhwa, to check its prevalence, determine its effect on the liver, kidney function tests and to check the role of supportive therapy. For this purpose blood sample was collected from 100 animals suspected to be suffering from Babesiosis in 20 different villages in district Swabi. Selection of animals was based on clinical signs like haemoglobinuria, temperature and jaundice of Babesiosis. The infection was confirmed by blood smears using Giemsa staining technique. The blood smears showed Babesia piroplasms, which were like double pear shaped or like signet-ring shaped. On the basis of microscopic examination the overall prevalence of Babesiosis in cattle in District Swabi was recorded as 10%. The blood of animals showing Babesia was analyzed in chemistry analyzer using commercially available kits.The values of SGPT, SGOT, Albumin, Total Bilirubin and Creatinine were increased while the values of Total protein an Albumin were decreased as compared to the normal reference values.
Bebesia positive animals Group A were divided into two Group B and Group C. Group B was only treated with antibabesial drug Imizol and Group C was treated with Imizol along with supportive drug i.e. liver tonic and diuretic (Hepasel+Lasix). After 5 days of treatment the plasma of treated animals was again analyzed for biochemical parameters and was compared before and after treatment (with and without supportive drug). The biochemical analysis showed that the biochemical indicators were normalizing in Group B and coming near to normal in Group C. The study is helpful in better understanding of the pathogenesis, supporting therapy and the effect of disease on the affected animals. This study showed that Babesiosis damaged the kidney and liver and the use of supportive drug along with specific drug was effective to recover the animals from infection.
Availability: Items available for loan: UVAS Library [Call number: 1267,T] (1).
13.
Epidemiology, Serodiagnosis And Chemotherapy Of Anaplasmosis In Cattle
by Farhan Ahmad Atif | Prof. Dr. Muhammad Sarwar Khan | Prof. Dr..Muhammad Arif Khan.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2011Dissertation note: Anaplasmosis is globally distributed tick-borne disease of livestock with great
economic importance in cattle industry. The current project was designed to estimate the
prevalence of anaplasmosis, ticks and risk factors associated with seroprevalence of
Anaplasma marginale among cattle in Sargodha, Khushab and Rawalpindi districts,
Punjab, Pakistan. Moreover, haematological changes in A. marginale infected cattle and
efficacy of chemosterilization regimens were evaluated using locally available drugs for
the elimination of adult naturally infected carrier cattle.
A total of 1050 blood, serum and tick specimens were collected from randomly
selected small holders (n=90) and private livestock farms (n= 12) using multistage cluster
random sampling technique. A total of 30 union councils, 34 cattle farms (30 small
holders and 4 livestock farms) and 350 cattle were selected as primary, secondary and
elementary sampling units from each district. Sampling unit was indigenous and
crossbred cattle of both the sexes.
Microscopic examination of the Giemsa stained blood mears revealed an overall
prevalence of blood parasites as 21.14%. Anaplasma marginale was the highe t prevalent
(5.81 %) haernoparasite of cattle followed in order by Theileria sp. (5.14%) and Babesia
bigemina (4.76%), respectively. Crossbred cattle were more susceptible to TBDs as
compared to the indigenous cattle. Highest prevalence of TBDs was recorded in summer.
The prevalence of tick-transmitted diseases was higher in small holders (31.3%) than
private livestock farms (17.5%). Chi square analysis indicated a significant association
(P<0.05) among indigenous and crossbred cattle to selected TBDs. Wherea. non-
significant association between different age groups, seasons, sex and farm sizes were
revealed.
The overall seroprevalence of Anaplasma marginale in cattle using cELlSA was
31.05%. Seroprevalence was higher in crossbred cattle of more than 4 years of age and
there was a significant (P<O.OO I) association between different age groups and breed. The
seroprevalence was significantly (P<0.05) higher in summer season in Sargodha and
Khushab districts. Moreover, the seroprevalence was significantly higher in small holders
in all study districts.
The epidemiological data and relevant information regarding area, host and farm
management factors were collected on a questionnaire through interview from each dairy
farmer, attendant or manager from September, 2009 to August, 20 10. Multivariate
analysis of risk factors revealed that cattle of more than 4 year of age (OR=5.42), heavy
tick infested (OR =2.10), crossbred (OR = 1.59) cattle were significantly at higher risk for
seroprevalence to Anaplasma marginale. Presence of Rhipicephalus (Boophilus)
microplus (OR=3.70), use of ivermectin (OR=3.97), moderate interval of acaricide
frequency (OR= 16.50), stall feeding (OR=4.90) and use of unhygienic needles
(OR=24.00) were significantly associated with seroprevalence to Anaplasma marginale
in cattle (P<0.05). The Sargodha district was at higher risk (OR = 1.81) as compared to
Khushab and Rawalpindi.
The tick species identified from cattle were Hyalomma anatolicum anatolicum,
Rhipicephalus (Boophilus) microplus, Rhipicephalus sanguine us, Rhipicephalus
(Boophilus) annulatus and Haemaphysalis sp. The overall prevalence of tick infestation
among cattle was 54.76%. The highest prevalence (57.71%) of cattle tick infestation was tick infested sites in cattle followed by dewlap (92%), inner thighs (90%), neck & back (54%), tail (26%), ears (13%), around eyes (10%), flanks (4%) and legs (2%).
The haematological changes were studied at different levels of parasitaemia " 7%, >7-15% and> 15%) in Anaplasma marginale infected Sahiwal and crossbred cattle. There was a significant difference (P<O.OS) among total erythrocyte count (TEC), total leukocyte count (TLC), haemoglobin (Hb), packed cell volume (PCV), mean corpuscular
haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) at different levels of rickettsemia in both breeds. ignificant difference (P<O.OS) was noticed among RBCs, PCV and MCH blood parameters between Sahiwal and crossbred cattle.
A total of sixty Anaplasma marginale seropositive adult Sahiwal cattle were selected having their ages between 3-4 years ranging in weight from 246-341 kg. The animals were divided in four groups designated as OXY -group-I, E RO-group-II, IMC- group-III and control-group-IV, comprising IS animals each. The seropositive animals received oxytetracycline (22 mglkg IV once in a day for five days), enrofloxacin (S mglkg IV once in a day for five days) or imidocarb (S mglkg 1M twice, 7 days apart). Re ult of chemosterilization study indicated that oxytetracycline 13/1S (86.67%) and irnidocarb
dipropionate II/IS (73.33%) eliminated Anaplasma marginale infection in adult naturally infected carrier cattle on S6th day. The carrier clearance was confirmed by cELISA followed by subinoculation of blood in seronegative splenectomized calves.
It was concluded that TTBDs are widely distributed in Punjab, Pakistan. Host. management and area factors are involved with the seroprevalence of Anaplasma marginale in cattle. Haemolytic anaemia is the major haematological finding of Anaplasma marginale in cattle. Oxytetracycline is more effective and safe In chemosterilization of persistent Anaplasma marginale infection in cattle. There is a need for country wide epidemiological studies on ticks and TBDs using advanced serological
and molecular techniques. Moreover, the identification of the potential vector of anaplasmosis should be required for the effective prevention and control of anaplasmosis in Pakistan.
Availability: Items available for loan: UVAS Library [Call number: 1368,T] (1).
14.
Isolation And Identification Of A Protease Producing Bacteria From The Decaying Plant Material And Investigation Of Its Potential As A Probiotic In Broiler
by Abdul Razzaq (2014-VA-498) | Mr. Shahid Abbas | Dr. Rahat Naseer | Dr.Muhammad Imran.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Biochemistry is gaining ground quickly due to the different preferences that it offers over customary chemical procedures particularly in regards to environment and cost contribution. Now a day’s industrial enzymes speak to the heart of biochemistry forms. Proteases are groups of organic catalysts. They have catalytic function.They hydrolyze peptide obligations of proteins and to separate them into polypeptides or free amino acids. Proteases are universal in nature and found in a wide range of differing qualities of sources, for example, plants, animals and microorganisms. Proteases do different organic procedures. Diverse types of proteases are available, alkaline proteases are a standout amongst the most imperative enzymes. The cost of the strain is a special in enzyme production and their applications in different industrial processes. The imaginative part of the present work was to distinguish and detach alkaline proteases from different organic sources, for example, from soil microorganisms with a reason to have beneficial outcome for hydrolyzing the proteins. The purified strain was checked for their potential part in chicken as a probiotics.
Protease producing bacteria act as probiotic to improve digestion which results in weight gain in broiler. In this research project, bacterial strains were isolated from decaying organic plants and soil samples collected from decaying organic plants of UVAS Lahore, Pakistan. Soil and decaying samples (1.0 g) were mixed in 100 ml normal saline, afterward serially diluted from 10−1 to 10−6 ratio with normal saline. 100 μl of each diluted sample inoculated in cultural medium and incubated at 37 °C for 48 h. The isolated colonies were selected to obtain pure bacterial cultures. Cultures were maintained in nutrient agar slants. Bacilli were isolated and screened from soil samples for production of proteases. The strains were identified morphologically, biochemically and on their molecular basis. The enzyme (protease) activity was assayed according to standard procedures by slight modifications. The strain was grown and fed to chicken as probiotic. The effects of probiotic were tested for growth of chicken. The probiotics improved the intestinal microbial balance and helped broiler in gaining weight. Bacterial stain studied in the current project may be used on commercial scale for promoting or enhancing the growth of broiler chicken.
Availability: Items available for loan: UVAS Library [Call number: 2717-T] (1).
15.
Physico-Chemical Factors Affecting In Vitro Stability And Activity Of Phytase From Indigenous Isolate Of Asperillus Therreus
by Safina kouser (2011-VA-422) | Dr. Aftab ahmad anjum | Dr.jawad nazir | Dr.Muhammad Yasir zahoor.
Material type: Publisher: 2017Dissertation note: Phytase is commercially important enzyme. Phytate in food and feed makes it less nutritive as well as acts as anti-nutritional agent. Phytate make complexes with important mineral ions and proteins. Monogastric animals and human are not able to degrade the phytate from plant based food because they lack phytase. This leads to phosphorous deficiency. Addition of phytase into food and feed degrades the phytate. It makes, phosphorous and mineral ions become available for growth and development. There is need to evaluate these factors in vitro which in real affect the stability and activity of enzyme under feed production process and digestive system of monogastric animals.
Indigenous Aspergillus terreus isolate produce stable phytase to be used in poultry feed.Indigenous strains of Aspergillus terreus were identified by macroscopic and microscopic characteristics. These isolates were screened on Phytate Screening Medium (PSM) for phytase production. Phytase producing A. terreus was than analyzed for toxin production through TLC (Thin layer chromatography). Non toxigenic phytase producing A. terreus isolates were inoculated in phytate broth for phytase production through submerged fermentation (SmF) under optimum conditions (28°C for 8-10 days). After centrifugation and filtration supernatants were used as crude enzyme. Phytase enzyme was qualitatively analyzed through phytase assay. Phytases activity units observed for isolate PAST-16 was highest (271.49±8.14 FTU/mL) and lowest (79.00±8.05FTU/mL) of PAST-05. A. terreus phytase (PAST-16) was subjected to temperature, pH and metal ions treatment.
Thermostability of phytases was recorted at 35°C, 55°C, 75 °C and 90°C for 15, 30, 45, and 60 minutes treatments. Enzyme from A. terreus (PAST-16) was observed as thermostable at
Summary
74
35°C, 55°C, 75 °C but not much stable at 95°C. Phytases showed 87.23±6.59, 198.34±4.47, 188.59±8.77 and 259.25±0.84 FTU/mL decreased in activity after 60 minutes of treatment at 35°C, 55°C, 75 °C and 95°C temperatures, respectively.
pH stability of phytases was found at pH of 2, 4, 6 and 8 for 15, 30, 45, and 60 minutes treatments. Enzyme from A. terreus (PAST-16) was observed as pH stable at 4, 6 and 8 but not much stable at 2 pH. Phytases showed 206.14±6.37, 169.59±6.37, 110.13±6.75 and 171.54±3.04 FTU/mL decreased in activity after 60 minutes of pH treatments at 2, 4, 6 and 8, respectively.
Metal ions effect on phytase activity was found with Ba2+, Ca2+, Cu2+, Fe3+, K+, Mg2+, Mn2+ and Na+ at the concentration of 1, 5 and 10mM. Enzyme from A. terreus (PAST-16) was observed as shows activity more with K+ less with Na+. Phytases showed 45.32±28.54 and 219.30±11.04 FTU/mL decreased in activity after 1mM conc. of K+ and 10mM conc of Na+, respectively.
Conclusion:
A.terreus isolate (PAST-16) produce stable phytase enzyme used in feed of poultry. In this way it tolerates condition under which feed process at commercial level and under digestive system monogastric animals. Availability: Items available for loan: UVAS Library [Call number: 2825-T] (1).
16.
Use Of Activated Carbon Prepared From Agricultural Wastes For The Adsorption Of Azo Dye.
by Qaisera Parveen (2011-VA-378) | DR.Rahat Naseer | Dr.Saeed Hashmi | Dr.Muhammad Asad Ali.
Material type: Publisher: 2017Dissertation note: Dyes are the visible pollutants of water and have toxicological and carcinogenic effects. So, dyes should be removed from aqueous solutions/industrial effluents. Many techniques are used for dyes removal from industrial wastewater discharge. From economical point of view, by the use of low cost adsorbents, basic dyes were removed from aqueous solution. For this purpose, adsorbents easily available were used to prepare activated carbon by physical and chemical treatment. Low cost adsorbents were prepared from Agricultural waste materials like sugarcane bagasse and sawdust for the adsorption of Azo dyes.
Agricultural wastes were treated by physical and chemical means for preparation of activated carbon. Characterizations of adsorbents were done by FTIR and SEM. FTIR micrographs showed that the different functional groups present on fibrous materials are responsible for adsorbing the MB. SEM showed the surface morphology. The effect of contact time, adsorbent dose, pH and temperature was studied in batch experiment. Freundlich and Langmuir adsorption isotherm was verified. Adsorption kinetic studies were done at regular time intervals using fixed amount of adsorbents. Equilibrium isotherms were studied by adding different adsorbent doses at 150rpm to attain equilibrium. Effect of pH was studied after balancing pH of the dye containing solution and analyzing the remaining dye in solution for equilibrium contact time. Effects of different parameters were evaluated and statistical analysis was applied to explore the most efficient adsorbent for methylene blue removal. as an adsorbent for Azo dye removal from wastewater without causing detrimental effects on environment. Since sugarcane bagasse and sawdust, an agriculture solid waste, used in the study, easily available, the adsorption process is expected to be economically implemented for wastewater treatment Availability: Items available for loan: UVAS Library [Call number: 2854-T] (1).
17.
Evaluation Of Pre And Post Training Food Safety Knowledge Of Food Handlers Working In Various Restaurants In Lahore
by Liaqat Ali(2009-VA-472 | Dr.Zubair Farooq | Dr.Sanaullah Iqbal | Dr.Muhammad Asad Ali.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Food-borne illnesses are common among people who eat food at restaurants. Most common reason of food-borne illness is cross contamination which occurs due to lack of food safety knowledge among food handlers of restaurants. Food hygiene is the set of fundamental standards utilized in an organized control of the environmental surroundings at the time of manufacturing, packaging, consignment/passage, storage, processing, preparation, selling and serving of food in such a way as to guarantee that food is intact to utilize and is of satisfying storing characteristics.
Therefore, there is need to evaluate their food safety knowledge and provide relevant training. There is lack of food safety knowledge among food handlers associated with restaurants. This study was designed to evaluate before and after training food safety knowledge of food handlers working in various restaurants in Lahore district. Nine restaurants each from nine towns of Lahore were selected. A minimum of two food handlers from each restaurant were further being selected. A total of 180 food handlers were put to pre-and post-training evaluation about food safety knowledge. Data was processed in Excel Spreadsheet. Paired t-test was applied using SPSS version 20 for comparison of variables. P ≤ 0.05 was considered statistically significant. A pair t-test was conducted to study the impact of pre and post training food safety knowledge of food handlers working in various restaurants in district Lahore. Almost all the results were significant which revealed that training has great impact in enhancing food safety knowledge of food handlers. All the results were significant at (P < 0.05). In first section, general questions about food safety, all results were significant. It shows different mean values of pre and post training and it revealed the impact of training on general food safety of food handlers. In personnel hygiene section, most of the results were significant, except the question “do you use safe water for cooking and cleaning?” because of reasons using safe water is the basic need and
CHAPTER 6
SUMMARY
SUMMARY
47
training do not have impact on it. In third section, questions related to foods or ingredients, all the results observed are significant. It shows means of before and after training are different and it shows that training has great impact on the knowledge related to food or their ingredients. In last section, question related to food machinery, most of the results are significant except “can the surfaces of all equipment be sanitized?” and “Do you use same knife or/and cutting board for cutting of vegetables, raw meat and fruits?” because of the reasons the surfaces of all the equipment’s cannot be sanitized due to lack of facilities available at restaurants and because it is a common practice to use same knife or cutting boards for cutting of fruits, vegetables and raw meat and also due to some financial issues, this shows training do not have impact on it. Present study is an effort to provided valuable information about the level of food safety knowledge of food handlers. Trainings conducted at different restaurants are helpful to increase the knowledge of restaurants food handlers regarding food hazards, cross-contamination and personal hygiene. Suggestions for Future Researchers
• Training materials (e.g., proforma, presentation) regarding food safety must be optimized according to Pulseberry Health Consultant and SGS Pakistan rules and regulations.
• Prior to the trainings, relevant documentation i.e., permission letter must be issued and verified from Punjab Food Authority to avoid any inconvenience. Availability: Items available for loan: UVAS Library [Call number: 2965-T] (1).
18.
Efficacy Of Levamisole, Benzimidazole And Ivermectin Against Nematodes In Sheep In District Jafferabad Balochistan.
by Mansoor Ahmad(2008-VA-161) | Dr.Muhammad Lateef | Dr.Saadullah Jan | Dr.Muhammad Oneeb | Dr.Muhammad Zubair Shabbir.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: The contribution of Livestock sector in agriculture’s share is about 11.6 % to national GDP out of which livestock share’s is more than 55 %. In Pakistan 70% of population live in rural area and nearly 35-40 million rural people directly or indirectly depend on livestock. The population receives about 30-40% of their income from livestock. The average population in each house hold is estimated to be 2 or 3 cattle, 5 to 6 sheep or goats. There are many hurdles and diseases that effecting the growth of livestock industry in the country, among these constraints parasitic infestation is one of the major growth threatening condition which affect the livestock the industry worldwide.
Many commercially available drugs are used every year to control the gastrointestinal nematodes in the animals, specially grazing animals. Uncontrolled uses of these medicines have made the helminth less susceptible to the drugs. And in many regions of world the parasites have developed resistance against such anthelmintic. To overcome the issue of drug resistance scientist is thinking to develop medicinal plant as curing agent against the nematodes.
The present study was designed to evaluate the efficacy of three commercially available anthelmintic against gastrointestinal of sheep of Tehsil Usta Muhammad district Jaffar Abad Balochistan. The drugs administered in the study were Albendazole, Levamisole, and Ivermectin. A total of 80 sheep microscopically positive and having worm load of > 150 eggs per gram of GIT nematodes were randomly selected for the trial. Four groups were made with each group containing 20 animals alternately. Group A was treated with Levamisole at the dose rate of 7.5 mg/kg body weight. The animals in group B were drenched with Albendazole at the dose rate
54
Summary
of 3.8mg/kg body weight. Group C were treated with Ivermectin at the dose rate of 0.2mg/kg body weight. In the results Ivermectin showed maximum reduction in EPG that is 96.17% followed by 91.71% and 84.17% of Albendazole and Levamisole respectively. The data was analyzed through repeated measure ANOVA using SPSS version 20.0. The results showed resistance against in the nematodes of sheep against the drugs Albendazole and Levamisole.
Conclusion
There is a serious threat of either developed or a tendency for development of anthelmintic resistance against Levamisole and Albendazole in nematode population of sheep in study area. The factors that assist to the development of anthelmintic resistance in nematode population of sheep flocks recorded in the present study can be divided in to operational, inherited, and medicinal, which need to be addressed together as a part of a potent worm control program. The level of alertness, awareness and technical know-how is unsatisfactorily poor among all the farmers concerned in small ruminant farming. Therefore, there is no dewormer schedule for worm control practices in place at present, which describes a severe fear on the future of sheep and goats production on the one hand and permits an immediate attention on the problem of anthelmintic resistance.
In the present study Ivermectin is commercially available and widely used anthelmintic is still effective against nematodes of sheep. Other anthelmintic were less effective in comparison to ivermectin in sheep of District Jaffarabad Balochistan. Availability: Items available for loan: UVAS Library [Call number: 2966-T] (1).
