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Dna Typing Of Saliva Stains Recovered From Date Pits

by Madiha kiran | Dr. M. Yasir zahoor | Dr. M. Imran | Ms. Asma waris.

Material type: book Book; Format: print Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2076,T] (1).

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Genetic Study Of Slc6a4 Gene In Convicted Offenders From Prisons Of Punjab, Pakistan Exhibiting Antisocial Personality Disorder Traits

by Asima Saman | Dr. Saadat Ali | Dr. M. Yasir Zahoor | Dr. M. Imran.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Antisocial Personality Disorder (ASPD) is characterized by incapacity of an individual to adapt themselves to social norms. Patients with ASPD typically have irritability problems and aggressive feelings toward other people. The serotonin transporter gene (5-HTT orSLC6A4) has been associated with regulation of serotonergic neurotransmission, mood and behaviour traits.Low levels of the neurotransmitter serotonin are believed to affect judgement, planning, and impulse control in ASPD sufferers. Genetic polymorphism in selected intronic region of serotonin transporter SLC6A4 gene was associated with antisocial personality disorder (ASPD) in convicted criminal of Punjab, Pakistan. We have selected two regions from SLC6A4 gene, which was intron 1 and exon 3.After extraction of DNA Polymerase Chain Reaction was used to amplify the extracted DNA of criminals (n=20) and control (n=10) for selected segments of intron 1 and exon 3 of SLC6A4gene. Sanger’s DNA sequencing method (di-deoxy chain termination method) was used to sequence the amplified fragments. Statistical and bioinformatics tools were used to analyse the data. Intron 1 has shown 5-HTTLPR polymorphism S allele (0.85 frequencies), LA allele (0.05 frequencies) and LG allele (0.1 frequencies) and exon 3 did not show polymorphism in criminal’s sample. The study highlights the role of SLC6A4 gene polymorphism in criminals of Punjab having antisocial personality disordertraits. Availability: Items available for loan: UVAS Library [Call number: 2576-T] (1).

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Mutational Analysis Of Atp7b Gene Responsible For Wilson’s Disease And Its Homology Analysis In Primates And Mouse

by Amama Ghaffar (2011-VA-375) | Dr. M. Yasir Zahoor | Prof. Dr. Huma Arshad Cheema | Dr. M. Imran | Dr. Amjad Riaz.

Material type: book Book; Literary form: not fiction Publisher: 2017Dissertation note: Copper being an essential element to carry out different cellular processes normally is maintained through proper regulation mechanisms to avoid its accumulation in the body. ATP7B gene that codes for ATP dependent P type ATP7B protein controls the regulation of copper in the body. It is required for the proper delivery of copper to apoceruloplasmin and its excretion through bile in the form of feces. Therefore, mutation occurring in the ATP7B gene can cause excessive cellular copper accumulation which results into Wilson’s disease. Variation in ATP7B gene related to copper transportation leads to Wilson’s disease and transmitted in generation through recessive pattern of inheritance. For this study blood samples of fifteen Wilson’s disease affected patients along with normal individuals of the same family were collected from Children's Hospital & Institute of Child Health, Lahore. DNA was extracted from blood through organic extraction method followed by DNA quantification. Amplification of exons 8, 13, 14 and 18 of ATP7B gene was performed after designing specific primers for these specific regions. Sequencing of amplified products was done through dideoxy chain termination method. A disease causing mutation of ATP7B gene c.3155 C>T; p1052 Proline (CCC) to Leucine (CTC) has been mapped on exon 14 in family with Wilson’s disease. This mutation can be used for genetic testing, prenatal diagnosis and genetic counseling. No mutation was found in exons 8, 13 and 18 which mean that further study needs to be done to find more local mutation(s) that can be used for fast direct genetic testing of Wilson’s disease patients or the carriers with heterozygotic conditions who can develop this disease at any age of their life. Results 87 MUSCLE and Clustal Omega were used for homology analysis of ATP7B gene nucleotide and protein sequences that revealed Gorilla to be closest to human regarding coding sequences, while Clustal Omega output file showed all the species varied highly in their protein structure homologies. Through the prediction of secondary structure homologies it was seen that marmoset was closest to humans. This study helped in providing prenatal diagnosis and genetic screening services in the country. It has facilitated in selecting animal models for further study and research on ATP7B gene and molecular pathogenesis of the Wilson’s disease leading to prevention and cure of disease. Availability: Items available for loan: UVAS Library [Call number: 2892-T] (1).

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