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51.
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Relationship Of Estradiol And Progesterone With Standing Estrus, Size Of Preovulatory Follicle And Interval To Ovulation In Beetal Goats

by Dr. Muhammad Irfan-ur-Rehman Khan | Dr. Muhammad Usman Mehmood | Dr. Muhammad Avais.

Material type: book Book; Literary form: not fiction Publisher: 2017Dissertation note: The objective of the current study was to determine follicular dynamics and plasma concentrations of ovarian steroid in response to single PGF2α, given randomly during the luteal phase in Beetal goat. A total of seven Beetal goats were given a single dose of PGF2α at the unknown day of luteal phase upon confirmation of corpus luteum via ultrasonography during the breeding season (November). Follicular dynamics were monitored using 7.5MHz transrectal transducer at every 12 h following PGF2α (0 h) until ovulation. Plasma samples for estradiol 17β and progesterone were collected at 0, 24, 36, 48, 60, 72, 84 and 96 h. An apronized buck was used at every 6 h to detect standing estrus. Relative to PGF2α, all goats (n = 7) exhibited onset of standing estrus at 50.6 ± 4.8 h and ovulation occurred at 82.3 ± 4.0h. However, the onset of standing estrus and ovulation staggered (P < 0.05) among the goats. The onset of standing estrus after PGF2α varied (P < 0.05) among early (n = 3), intermediate (n = 2) and late (n = 2) responding goats i.e., 44 ± 2.0 vs. 51 ± 3.0 vs. 60 ± 0 h, respectively. The ovulation time among early, intermediate and late responding goats was 72 vs. 84 vs. 96 h, respectively. The peak plasma concentration of estradiol 17β was observed 12 h prior to ovulation in the goats. Mean diameter of ovulatory follicle and duration of standing estrus were similar among the groups. The corpus luteum regressed rapidly following PGF2α in early responding goats followed by intermediate and late responding goats. Although plasma concentration of progesterone did not differ (P = .065) among early, intermediate and late responding goats, but the change in progesterone concentration over time differed (P < 0.05) among the groups. In conclusion, this study indirectly shows that the onset of standing estrus and interval to ovulation following PGF2α may vary in Beetal goats due to follicular and hormonal dynamics during the luteal phase. Availability: Items available for loan: UVAS Library [Call number: 2947-T] (1).

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52.
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Effect Of Antibiotic Treatment During Ovulation Synchronization In Repeat Breeder Holstein Friesian Cows

by Masood Shabbir (2010-VA-120) | Dr. Muhammad Zahid Tahir | Dr. Amjad Riaz | Dr. Muhammad Avais.

Material type: book Book; Literary form: not fiction Publisher: 2017Dissertation note: Repeat breeding is one of the major causes of reproductive, productive and economic losses in dairy sector. The main causes of repeat breeding include sub-clinical infection of reproductive tract, age of the animal, error in the detection of estrus, endocrine dysfunction and nutritional deficiencies. In Pakistan, a very high incidence of repeat breeding has been documented (Kakar et al. 1997). In the past, intrauterine infusions have successfully been used with a variety of antiseptic and antibiotic solutions. Meanwhile, therapeutic use of GnRH and PGF2α has also been demonstrated to result in improved pregnancy rates. In particular, Ovsynch protocol leads to an increase of 6-17% in conception rate. The objective of the present study was to evaluate the reproductive efficiency with antibiotic treatment during ovulation synchronization in repeat breeder Holstein Friesian cows. This study was conducted on 30 pure bred Holstein Friesian cows kept under standard farm conditions of feeding and management at Hussain Cattle and Dairy Farm, Kasur. The reproductive efficiency of treated and control animals was based on ovulation rate, non-return rate, conception rate, pregnancy rate, embryonic losses and luteal function. Results were analyzed by independent T-test. A probability level of 95% was consider as significant (P<0.05). All the experimental animals were screened to confirm non-pregnant and normal genitalia. Both the treatment and control groups were synchronized using ovsynch protocol. Following first injection of GnRH, the treatment group was subjected to intrauterine antibiotic infusion for five days. On Day 7 of protocol both groups received an injection of PGF2α. At day 9 of ovsynch protocol before second injection of GnRH both groups were scanned for ovarian status. Follicles and CL measurement were noted and mapped by using 7.5 MHz trans-rectal probe (Honda 22 Summary Model HS-1600) in both groups and second injection of GnRH was given. Timed artificial insemination was performed after 16-20 hours of second injection of GnRH in both groups. After 8 hours of artificial insemination again ultrasonography was conducted to check the ovulation rate in both groups, there was no significant difference between control and treatment, 60% and 75% of the animals in control and treatment groups ovulate respectively. At day 11 blood samples were collected for progesterone assay. Non-return rate was visually observed at day 18 to 21 of artificial insemination. Blood was collected at day 18 for progesterone assay by puncture of tail vein. After D28 andD42 of artificial insemination first and second pregnancy test were conducted by using 7.5 MHz trans-rectal probe (Honda Model HS-1600) in control and treatment group. There was a significant difference of pregnancy among the control and treatment group by independent T-test. 3 rd and 4 th blood samples were collected for progesterone assay at day28 and 42 of artificial insemination in both groups respectively. Availability: Items available for loan: UVAS Library [Call number: 2951-T] (1).

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