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1. Geographical Distribution Of Dengue Fever Infected Patients And Its Related Risk Factors In And Aroun Lahore.

by Muhammad Usman Zaheer | Dr. Muhammad Hassan Mushtaq | Prof. Dr. Mansur-ud-Din Ahmad.

Material type: book Book; Format: print Publisher: 2012Dissertation note: Dengue fever is the most common mosquito-borne viral infection of humans worldwide and is a major public health concern, especially in tropical and subtropical regions (Mackenzie et al., 2004). This life threatening disease is caused by a specific virus (ARBO-Flavivirus) transmitted by the bite of a female mosquito (Aedes aegyptii and Aedes albopictus) which is identified by the white bands and scale patterns on its legs and thorax. Trans-ovarial transmission of dengue virus has also been reported in different studies. The mosquito may bite at any time but the probability of bites increase at dusk and dawn (WHO 2009). Dengue fever has emerged as a significant public health problem in Pakistan. In Pakistan, the first epidemic of dengue fever was reported in 1994 in Karachi (Anaari et al., 2001). Several years later, in 2006 another epidemic was reported in the southern part of Pakistan, predominantly caused by DENV-2 that led to 58 deaths (khan et al., 2008). In 2007, 22 people died of disease and in 2008 only five people were reported to die from it. Between October 2006 and January 2008, there were 3,242 laboratory confirmed cases of dengue fever in Pakistan. Since January 2009, 224 suspected cases were reported in Sindh province, of which 135 were the confirmed cases. During the epidemic of dengue fever in 2010 more than seven thousand cases were reported in Pakistan, of which 5 thousand cases were from Punjab and resulted in 63 deaths. The mosquito-borne viral hemorrhagic diseases are prevalent throughout the world. Dengue hemorrhagic fever (DHF) is one of the most important diseases widely distributed in the tropics of South East Asia. It is an infectious malady of human beings characterized by fever and non-specific constitutional symptoms. The virus is transmissible to human beings by the bite of female Aedes aegypti and Aedes albopictus. The disease is spreading like a geometric rise of cases in a wide range of geographical regions. The geographical factors and locality of patients may be a contributing factor to the outbreak of Dengue Fever. Climate change is likely to increase the area of land with a climate suitable for dengue fever transmission, and that if no other contributing factors were to change, a large proportion of the human population would then be put at risk. The data on address of patients were collected from all the patients admitted in tertiary care hospital from August 01, 2011 to November 30, 2011. The geographical risk factors studied in this project were presence or absence of parks, trees, ponds, swimming pool, service station, tyre shop, source of stagnant water, pond and municipality garbage tank in vicinity of house of the cases and controls. For the study of risk factors we made 2 groups i.e. cases and controls; each comprising of 300 subjects and then data on presence or absence of various hypothesized risk factors in the vicinity of house (400 meters) and area (800 meters) were collected through a well structured and pre-tested questionnaire (Harrington et al., 2005; Honório et al., 2003; Schatzmayr 2000; Muir and Kay 1998; Reiter et al., 1995; McDonald 1977; Hausemann 1971 and Bond et al., 1970). The statistical analysis of data was done by using SPSS 16.0. Qualitative data were presented in form of tables, bar charts, column and pie- charts. Chi-square test was applied to see the association between dengue fever and various hypothesized risk factors. P-value<0.05 was considered as significant. Odds ratio was calculated at 95% level of confidence (CI) for various hypothesized risk factors of dengue fever. The results of present study revealed that the season of dengue fever in Pakistan is from August to November and the period prevalence of dengue fever to be 0.11%. Males (59%) were mainly the victim of dengue fever. The main affected age groups were 16-30 year (37.33%) and 31-45 years (29.67%). As far as the geographical distribution of dengue fever infected patients is concerned, it was concluded that dengue fever cases were reported from all the ten towns of Lahore metropolitan, however, most of the cases were reported from Ravi town followed by Data Ganj Baksh town and Cantt. The results regarding various hypothesized risk factors are as follows: parks/trees/plants in vicinity of house OR=8.81, p-value <0.05; swimming pool in vicinity of house OR=5.O3, p-value <0.05; source of stagnant water in vicinity of house OR=8.25, p-value <0.05; fogging in your area OR=0.50, p-value <0.05; service station in the area OR=5.98, p-value <0.05; tyre shop in the area OR=6.11, p-value <0.05; municipality garbage tank in the area OR=5.88, p-value <0.05; any pond in the area OR=5.69, p-value <0.05. From the results of this project we concluded that dengue fever is most prevalent from August to November and it is a disease of mainly male gender and mostly affects people of age group 16-45 years. Besides this, dengue fever is almost equally distributed in all the towns of Lahore metropolitan. Also we concluded that presence or absence of parks, trees, plants, swimming pool, stagnant water, pond, service station, tyre shop and municipality garbage tanks in vicinity of house are the risk factors of dengue fever. Fogging proved to be a deterrent to dengue fever. On the basis of results of this project there is need to implement a control program in which vector control and minimizing the exposure of people to risk factors should be the key points that need to be addressed. Availability: Items available for loan: UVAS Library [Call number: 1444,T] (1).

2. Prevalence Of Antibiotic Resistant Staphylococcus Aureus In Milk, Milk By- Products And Food Handler At Retail

by Kanwal Hafeez | Prof. Dr. Mansur-ud-Din Ahmad | Dr. Muhammad Hassan Mushtaq.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2012Dissertation note: The present study was conducted to check the prevalence of antibiotic resistant Staphylococcus aureus in milk, milk products and food handlers at various dairy retail shops in Lahore. A total of 100 samples, 30 samples of raw milk, 30 samples of pasteurized milk, 20 samples of yogurt, 10 samples of butter and 10 hand swabs samples of dairy handlers were analyzed. Staphylococcus aureus was detected in 54% (54 out of 100) samples. Overall high prevalence was recorded in raw milk, followed by hand swabbing samples of dairy handler, butter, pasteurized milk and yogurt. The results indicated unhygienic practices during milking and handling of milk at different critical control points such as transportation, storage, and post-pasteurization contamination. Overall high prevalence of ampicillin and amoxicillin resistant Staphylococcus aureus were recorded in all the samples of milk and milk products and the hand swabbing of dairy handlers, followed by resistant against chloramphenicol, trimethoprim, streptomycin and vancomycin respectively. All the isolates were resistant to ampicillin followed by resistance against amoxicillin, chloramphenicol, trimethoprim, streptomycin and vancomycin which were 92.6%, 37%, 33.3%, 24.1% and 22.2% respectively. The antibiotic resistant Staphylococcus aureus in milk and milk products indicated the widespread use of these antibiotics in treating different types of infections associated in either livestock or humans which results in selection pressure of these antimicrobials and results in the emergence of these antibiotic resistant bacteria in different environmental conditions. The risk associated with human health as a result of ingestion of these antibiotic resistant bacteria can be prevented by adopting hygienic measures during milking, transport, storage and hygienic handling by the handlers so the human health can be assured. Availability: Items available for loan: UVAS Library [Call number: 1520,T] (1).

3. Detection Of Carrier And Subclinical Infection Of Babesia Ovis Trough Pcr At Government Farms Of Punjab

by Haider Noor | Prof. Dr. Mansur-ud-Din Ahmad | Dr. Muhammad Hassan Mushtaq.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2012Dissertation note: Haemoprotozoan infections in ruminants are of significant importance in tropical and subtropical regions of the world. Diseases are transmitted through ticks thus a number of epidemiological factors/risk factors are involved. Ovine Babesiosis is one such disease posing problems in Pakistan which is an agricultural country and livestock plays an important source of income for farmers. The economic losses in small ruminant production are significant in the tropical and subtropical regions of the world. Carrier sheep infected with Babesiosis are challenge to current diagnostic methods and are difficult to detect because of the low number of parasites in circulation. However diagnosis of carrier animals in herd is important for preventing outbreaks by transmission through vector ticks to healthy animals and for obtaining epidemiological data of disease. The work done on Babesia ovis is negligible. For this purpose a study was conducted at two farms to measure the prevalence and optimization of PCR for Babesia ovis. Blood was collected into an anticoagulant containing vacutainer. First thin smears were formed and stained with Giemsa stain for microscopic examination of Babesia ovis. For DNA extraction Puregene DNA purification system, Gentra, was used. Extracted DNA was amplified in a thermolyser using B. ovis primers and then analyzed using electrophoresis on 1% agarose gel. Microscopic examination demonstrated a prevalence of 16 % while PCR results revealed prevalence of 29% for B. ovis. Results displayed that the efficacy of PCR is more sensitive than Light Microscopy. Data on infection rate between male and female and between different age groups was statistically non-significant. Herd wise prevalence was 36% and 22% in Livestock Production Research Institute, Bahadarnagar, Okara and Small Ruminant Training and Research Center,Ravi Campus, Pattoki respectively. Common sites of attachment for the ticks were under the tail, perineal region and underneath ears.The data was analyzed by using SPSS (Statistical Package for Social Sciences). Availability: Items available for loan: UVAS Library [Call number: 1534,T] (1).

4. Seroprevalence Of Toxoplasmosis In Goats And Their Attendants In Dort Minro (D.G.Khan), Punjab (Pakistan)

by Shams-ur-Rehman | Prof. Dr. Mansur-ud-Din Ahmad | Dr. Muhammad Hassan Mushtaq.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2013Dissertation note: Toxoplasmosis is a protozoan disease of cats, however all warm blooded animals comprising mammals, birds and human beings can be infected with it. The disease is manifested by abortion, resorption, neonatal loses, birth of weak kids, birth defects and mummification of fetuses in pregnant animals. Global seroprevalence of Toxoplasmosis is continuously evolving in animals and human beings. Keeping in view the zoonotic importance of Toxoplasmosis, the present study was conducted, in Fort Minro, to determine the seroprevalence and potential risk factors of Toxoplasmosis in goats and their attendants. A total of 52 pastoral goat herds with herd size of "?20 - ?40" animals per herd were selected by convenient sampling technique. Out of these 52 pastoral herds, 237 goats were selected by systematic sampling method i.e each 5th unit was selected for sampling. The goats were sampled if the age of goats was ?6 months. Most common breeds of these herds were, Pahari and Hairy goat breed. A total of 92 herd attendants were also selected to determine the zoonotic potential of Toxoplasmosis in Farm workers / attendants. All serum samples were screened by LAT. For this purpose, the commercial Latex agglutination kit (Wiener Laboratorios S.A.I.C. Riobamba 2944, 2000 Rosario Argentina) was used for detection of specific antibodies of T.gondii in serum of goats and their attendants. A herd was considered as a positive, if at least one goat was found to be positive in that specific herd. Out of total 52 goat herds, 36 herds presented at least one or more than one seropositive animal. The number of positive animals in each herd, ranged from 1-10 animals. Out of 237 goats, 64 goats were positive for Toxoplasma gondii antibodies. Thus overall seroprevalence of Toxoplasmosis in goats was 27%. The seroprevalence was significantly high in female goats (21.5%) as compared to males (5.5%). Kajli/Pahari goat breed was more positive (14.8%) as compared to Hairy goats (12.2%). An increase trend of seropositivity was observed in goats with increase in age. Age group of "?3 years" of goats had highest share of percentage prevalence to overall prevalence of Toxoplasmosis, followed by age group "?1-<3 years", while age group "?6 m-<1Year" had lowest share of percentage prevalence to overall prevalence. Prevalence in males had 5.5% share in overall prevalence (27%) while prevalence in females had 21.5% share in overall prevalence. Data analysis of various risk factors determined that natural reservoirs of water, pastoral grazing and presence of cats and dogs were major risk factor of Toxoplasmosis in goat herds. Access of cats to drinking water and food resources and eating medium cooked meat were major risk factors for goat attendants. High prevalence of Toxoplasmosis in goats and Farm workers, suggests that Toxoplasmosis is endemic in the study area and further epidemiological studies should be focused on goat meat, milk and milk products. It is concluded that Toxoplasmosis can be reduce in human beings and animals by reducing risk factors and maintaining proper hygienic measures. Availability: Items available for loan: UVAS Library [Call number: 1710,T] (1).

5. Studies On Risk Factors Associated With Transmission Of Escherichia Coli O157: H7 In Abattoir Environment

by Samiullah Khan | Prof. Dr. Mansur-ud-Din Ahmad | Dr. Muhammad Hassan Mushtaq.

Material type: book Book; Format: print Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1711,T] (1).

6. Association Of Antimicrobial Resistant Campylobacter With Poultry Meat At Retailer Shops In Lahore Through Cross-Sectional Study

by Abdul Moeed | Prof. Dr. Mansur-ud-Din Ahmad | Dr. Muhammad Hassan Mushtaq.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2013Dissertation note: 6.1 Background Antimicrobial resistance is an under-appreciated threat to public health in nations around the globe. With globalization booming, it is important to understand international patterns of resistance. Thermotolerant Campylobacter spp. are considered to be the most frequent cause of human acute bacterial enteritis worldwide, furthermore, numerous transmission vehicles are known, but raw milk, untreated surface water and especially poultry meat are major sources of human infections. In Pakistan studies reveal that among meat samples, the highest prevalence (48%) of Campylobacter was recorded in raw chicken. Therefore, the increasing level of resistance to the antibiotics among Campylobacter spp. is recognized as emerging public health problem. 6.2 Hypothesis " Campylobacter spp. was present in the poultry meat at retailer shops. " Unhygienic measures concerning to meat handling had a considerable effect on the prevalence of antimicrobial resistant Campylobacter. 6.3 Methodology A cross sectional study of 3 months duration was carried out in the Lahore city of Punjab, Pakistan. Using the standard microbiological techniques and statistical analysis, the prevalence of Campylobacter and its pattern of antibiotic resistance were evaluated. Furthermore, through premeditated questionnaires, public awareness regarding meat-borne diseases, food safety and inappropriate usage of antibiotics were assessed as well. 6.4 Statistical design Data will be analyzed by using SPSS version 20.0. Quantitative variable were presented by using mean ± S.D. Qualitative variable were presented with help of frequency tables, pie charts and bar charts. Chi-square test was applied to see the association among different variables. P value <0.05 was be taken as significant. 6.5 Outcome Through this cross-sectional study, the prevalence and pattern of the antibiotic resistant campylobacter was assessed; moreover public awareness with special reference to food safety, knowledge of meat borne diseases and usage of antibiotics wereevaluated. Furthermore, one could play an influential role in public health by dissemination of awareness among the masses about the proper usage of antibiotics and food safety measures. Availability: Items available for loan: UVAS Library [Call number: 1728,T] (1).

7. Nosocomial Infections At Intensive Care Unit In A Tertiary Care Hospital In Lahore City

by Ajmal Iqbal | Prof. Dr. Mansur-ud-Din Ahmad | Dr. Muhammad Hassan Mushtaq.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1729,T] (1).

8. Prevalence Of Salmonelia Ennteritidis In Hatcheries And Broiler Retail Outlets In Rawalpindi Punjab

by Abdul saeed | Dr. Muhammad Hassan mushtaq | Prof Dr. Mansur-ud-din ahmad.

Material type: book Book; Format: print Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1868,T] (1).

9. Epidemiological Investigation And Risk Factor Analysis Of Brucellosis In Large Ruminants And Their Attendants At Govt. Livestock Farms In Punjab

by Muhammad Raashid (2007-VA-496) | Prof. Dr. Mansur-ud-Din Ahmad | Dr. Muhammad Hassan Mushtaq | Dr. Sehrish Firyal.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Pakistan has been renowned as an agricultural country. It is rich in livestock sector having fairly large populations of domestic animals. Among these, populations of cattle and buffalo are 38.3 and 33.7 million respectively. The importance of cattle and buffalo cannot be denied at any level as these are the principal farming animals and milk and beef are widely consumed locally in the country. The estimated annual milk production of cattle and buffalo include 17.372 and 30.462 million tonnes respectively and a combined 1.829 million tonnes beef for human consumption during 2012-2013 (Pakistan Economic Survey 2012-2013). Brucellosis, a worldwide bacterial zoonosis, is one of the most serious diseases causing huge loss to national economy and human beings among developing countries (Wu et al. 2013). The disease is endemic in Middle East, Central Asia, Africa, Mediterranean region and parts of Latin America (Gwida et al. 2010). Brucellae are Gram-negative bacteria, facultative anaerobic and intracellular pathogens. These show a wide range of host specificity. These coccobacilli measure from 0.6 to 1.5 µm long and 0.5 to 0.7 µm wide. Eight species have been identified in the genus Brucella such asBrucella abortus(B. abortus affecting cattle and buffalo), B. melitensis (sheep and goats), B. ovis (sheep), B. suis (swine), B. canis (dog), B. neotomae (desert rats), B. ceti (cetaceans) and B. pinnipedialis (pinnipeds) (Blasco 2010). This wide range of species covers almost all domestic animals however cats have found resistant. Generally it is considered as a reproductive problem in both male and female animals (Ficht 2003). Brucellosis has been listed as the second most serious zoonotic disease in the world after rabies by OIE (OIE 2009). B. abortus, the primary cause of Brucellosis in large ruminants, (cattle and buffalo), remains not only a significant threat as a source of human illness but also risks economy of the country (Makita et al. 2011). Present estimates of economic loss in meat and milk production resulting from Brucellosis are $800 million annually in the United States(OIE 2009). The incidence of the disease can be correlated to several factors including demographic and geographic factors(Soomro et al. 2014). Seroprevalence of the disease has been reported in different regions of Pakistan and ranges 3.25 to 4.4%(Naeem et al. 1990). Brucellosis in cattle and buffaloes can be recognized clinically by an abortion usually occurring form 6 months and onwards i.e. last trimester of pregnancy (Soomro et al. 2014). Brucellosis is principally a disease of sexually mature animals as it affects mainly the reproductive system and fertility of the animals. It significantly reduces the survival rate of newborns and also the milk yield (Sikder et al. 2012). Greyish white mucoid or mucopurulent discharges from the vagina, prior to parturition of cow, may show the clinical patterns of disease along withnormal patterns of parturition like swelling of the vulva, relaxation of pelvic ligament, enlargement of udder and discharge from the vulva (Shafee et al. 2012). Human infections as a result of Brucellosis range more than 500,000 annually round the world (Abo-Shehada and Abu-Halaweh 2011). Brucellosis can cause a wide range of symptoms similar to the flu and may also include fever (39-40°C), night sweats, headache, back pain and physical weakness. Severe form of infection may result in involvement of the central nervous system or the lining of the heart (Soomro et al. 2014). It is one of the principal public health problems for an agricultural country like Pakistan, where majority of the population is engaged in livestock farming (Shafee et al. 2012). Brucellosis in humans is a severely debilitating condition that usually requires prolonged treatment involving a combination of strong antibiotics. The treatment results in permanent and disabling sequel, and also in significant medical expenses along with loss of income due to loss of working hours. Brucellosis can be transmitted to humans by ways of inhalation, direct contact with infected animals or contaminated products of conception and ingestion of unpasteurized dairy products and undercooked meat or meat products (Gwida et al. 2010; John et al. 2010). Brucellosis can also be transmitted from infected animals to human beings who are in close contact with animal secretions like infected vaginal secretions, blood, urine, feces, aborted fetus, or those who consume unpasteurized milk or other raw milk products. Shepherds, milkmen, butchers, knackers, veterinary assistants, and abattoir workers are at high risk (Agasthya et al. 2007) Availability: Items available for loan: UVAS Library [Call number: 2245-T] (1).

10. Isolation And Molecular Detection Of Salmonella Species In Milk And Milk Products

by Muhammad Umar Ijaz (2007-VA-040) | Prof. Dr. Mansur-ud-Din-Ahmad | Dr. Muhammad Hassan Mushtaq | Dr. Ali Ahmad Sheikh.

Material type: book Book; Literary form: not fiction Publisher: 2015Dissertation note: Salmonellosis is one of the emerging zoonotic diseases. Human infections result from contaminated raw or undercooked food. Various sources of contamination in milk are dirty udder, unhygienic handling and utensils, dirty hands of milkmen and poor quality water supply at the farm. To ensure food safety there is a dire need for the availability of valid, rapid and accredited diagnostic system. Conventional culture methods for detecting Salmonella spp. in foods needs 4–5 days. The development in molecular techniques made it possible to cut short the procedure. The present study was conducted with the aim to evaluate the burden of Salmonella spp. in milk and milk products. In total 150 samples were collected, out of which there were 75 milk samples. These samples were collected from farm dairy herds (pooled milk) located in Harbanspura Dairy colony, Lahore. Salmonella was detected in 8 samples (11%) by conventional method. In addition to milk samples, 75 samples of milk products were also collected from retail shops. These include 50 samples of Yogurt and 25 samples of Yogurt milk. Milk products had 6 positive samples (8%). No sample of Yogurt was detected positive for Salmonella. The reason for this is that Yogurt had only Salmonella if it is present in starter culture. Furthermore yogurt is made after frequent heating. Salmonella is killed by such continuous and vigorous heating. Yogurt milk is prepared from Yogurt along with raw milk in a separate utensil. There are chances of contamination there so it had 6 positive samples. Total 14 (9%) samples were detected in 150 samples by conventional method. It was found that Salmonella was present in raw milk samples and milk products at places where either cleanliness was not proper, milk handling practices Summary 53 were not up to the mark, udder was not washed before milking or overall management practices were poor. Positive samples obtained by conventional method were further analyzed by PCR for Salmonella genus confirmation. Out of total 8 positive milk samples by conventional method, 5(62%) were confirmed as Salmonella genus. Similarly from 6 positive samples of milk products by conventional method, only 2(33%) were confirmed as of Salmonella genus. Further PCR was conducted for Salmonella typhimurium detection. Milk samples were containing 3(60%) positive samples while no positive sample was detected in milk products. On the basis of the results of present study, it is recommended that milk should always be boiled before use. Further Hygienic measures should be adopted during milking. Proper handling of milk and milk products is also important to limit the cross contamination. It is also advised that standards such as pasteurization and HACCP should be introduced to facilitate production of good quality milk. Electronic and print media should also create awareness among people about food safety. Availability: Items available for loan: UVAS Library [Call number: 2408-T] (1).

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