1.
Effect Of Different Physiological Stages On Heamatological And Biochemiscal Parameters Of Nili-Ravi Buffalo And Sahiwal Cattle
by M. Zohaib Aslam | Dr. Imtiaz Rabbani | Dr. Muhammad Shahbaz Yousaf | Mr. Muhammad.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: 6.1 Background
The proper disease diagnosis in large ruminants is required for overcoming the economy loss due to livestock poor management. Blood biochemical and hematological parameters are one of an important medium to assess the physiological and pathological condition of animals. Any physiological and pathological change can be evaluated only if the normal values are available for comparison. Factors such as age, breed, geographical location, diet, sex, diurnal variation, physical activity, pregnancy and environment are known to influence the serum biochemistry and haematology of large ruminants. Our hypothesis was to evaluate the effect of different physiological stages (pre-pubertal and pubertal) on hematological and serum biochemical parameters of Nili-Ravi buffalo and Sahiwal cattle.
6.2 Materials and Methods
Sixty blood samples (30 Cattle and 30 Buffalo) were collected from Ravi Campus Pattoki, UVAS and divided on the basis of different physiological stages, pre-pubertal (10-12) and pubertal (22-24 months) into two groups. The collected samples used to estimate complete blood counts by hematological analyzer. The biochemical parameters include cholesterol, triglycerides, total protein, urea, AST and ALT by commercially available kits (Biogene and crescent).
6.3 Statistical Analysis
Statistical Package for Social Science (SPSS) version 17 used. Further T Test was used to compare the hematological and blood biochemical between two age groups. Differences was considered significant at P<0.05 (Steel et al.1997).
6.4 Results
The hematological parameters RBCs, Hb and PCV were showed a significant difference at Pre-pubertal and pubertal stage of both buffalo and cattle. Whereas the WBCs count, lymphocytes, granulocytes, monocytes, MCV and MCH were not found significantly different at pre-pubertal and pubertal stages of buffalo and cattle. The serum level of glucose recorded highest and urea level lowest at pubertal stage in both buffalo and cattle. The cholesterol and triglyceride were found significantly (p<0.05) lowest, while liver enzyme AST and ALT were found significantly highest with the aging of animal in both species. No significant changes were observed in total protein level at pre-pubertal and pubertal stage in buffalo and cattle.
6.5 Conclusions
The WBCs, RBCs, Hb, PCV were found significantly different between pre-pubertal and pubertal stages in both species. The values of theses parameters recorded highest at pubertal stage, while granulocytes, lymphocytes, monocytes, MCV and MCH were found non-significantly different. Different physiological stages had a significant effect on glucose, cholesterol, triglycerides, Urea, ALT and AST in both species. The results of this study indicated that different physiological stages must be taken as very important factor in determining the blood parameters.
Availability: Items available for loan: UVAS Library [Call number: 1806,T] (1).
2.
Effect Of Age And Gender On Hematological And Biochemical Parameters Of Beetal Goat And Lohi Sheep
by Sidra Shafique | Dr. Imtiaz Rabbani | Dr. Muhammad Shahbaz Yousaf | Prof. Dr. Khalid.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2014Dissertation note: Small ruminants are one of the domestic animals of the inhabitants of Pakistan. In semi-arid and arid areas goat and sheep provides the main means of survival and security. So the proper health management of animals is must require for their survival. Hematlogical and biochemical tests are widely used for the diagnosis of serious animal diseases which can lead to economics losses in animals like reduced fur, wool and milk production. A large number of factors such as sex, age, gender, breed, illness, stress, seasonal variation and nutrition can affect the pattern of these clinical values. Our hypothesis was to evaluate the effect of age and gender on hematological and biochemical indices of Beetal goat and Lohi sheep in Ravi campus Pattoki UVAS.
One hundred and twenty blood samples (60 Goats and 60 sheep) were taken and divided on the basis of their age and gender (0-3 months, 9-12months and 18-20 months). Complete blood counts were performed by hematology analyzer (Abacus junior vet. Serial # 130076, Diatron GmbH wein, Austria) at W.T.O, UVAS. The biochemical parameter included albumin, globulin, cholesterol, triglycerides, total protein, urea AST and ALT were determined by commercially available kits (Biogene® and Crescent®). The arithmetic means (± SE) of hematological and biochemical parameters in different groups were calculated. The arithmetic means (± SE) of hematological and biochemical parameters in different groups were calculated. The collected data were subjected to two way analysis of variance through statistical package SPSS version 13 to evaluate mean differences in hematological and blood biochemical parameters of different age groups. Difference among the means of groups with p<0.05 was considered as significant through LSD test (Steel et al.1997).
In this study age and gender had a remarkable influence on the pattern of hematological and biochemical values of both sheep and goat. The RBCs, Hb, PCV, MCV, MCH, MCHC, WBCs and lymphocytes counts found significantly (p<0.05) higher in animal of age 18 to 20 months as compared to other groups in both species, While MCHC, monocytes and granulocytes found non-significantly (p>0.05) different. Male showed statistically (p<0.05) higher RBCs, Hb, PCV and MCV values as compared to female. The glucose, cholesterol, triglyceride, urea, AST, ALT, Total protein, albumin and globulin were found significantly (p<0.05) different by the aging of animal. Gender had no significant (p>0.05) influences on biochemical parameters of both sheep and goat.
Conclusion
Based on these findings age and gender had significant influence on hematological and biochemical parameters. In general veterinary practices, body weights are usually considered for therapeutic and nutritional strategies. Often too young animals are treated with more caution. In conclusion it can be mentioned that not only body weight but also age of the animals should be considered for devising therapeutic and nutritional strategy in livestock.
Availability: Items available for loan: UVAS Library [Call number: 1814,T] (1).
3.
Effects Of Dietary Supplementation Of Moringa Oleifera Leaf Powder On Redox Status, Hepatic And Kidney Function In Rats Exposed To Heat Stress
by Noor Muhammad Khan (2015-VA-07) | Dr. Khalid Abdul Majeed | Dr. Muhammad Shahbaz Yousaf | Dr. Hafsa Zaneb.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: here are a number of stressors which adversely affects the normal homeostasis of the body. One of them is heat stress which has marked damaging effect on the normal physiology of an individual. Moringa oleifera is a versatile plant from Moringaceae family. Moringa oleifera is a best food plant but it also has a large potential as a medicinal plant. Due to rich source of flavonoids, Beta- carotenes, vitamin A & D, Moringa oleifera is known as a best natural anti-oxidant. The leaves of Moringa oleifera also possess hepato-protective and renal protective activities due to its rich anti-oxidant potential.
Hypothesis:It was hypothesized that Moringa oleifera leaf powder supplementation will ameliorate the redox status, hepatic and kidney function in rats exposed to heat stress.
Methodology: Adult male wistar rats (n= 24) were procured and housed in stainless steel cages in the experimental animal shed of department of Physiology, University of Veterinary and Animal Sciences, Lahore-Pakistan. After acclimatization, rats were divided in to four groups (One control and 3 MOLP treated groups). Rats were exposed to high environmental temperature (37±1°C ) for one hour daily(12.00 PM-1.00 PM) throughout the experimental period of 21 days for the induction of heat stress(Sinha. 2007). Anti-stress properties of Moringa oleifera leaf powderwere examined by measuring biochemical parameters, i.e. redox by estimating serum malondialdehyde through assay used by(Ohkawa et al. 1979) and catalase by the same spectrophotometric protocol in accordance with the study (Hadwan et al. 2016). Analysis of liver function enzymes (Aspartate aminotransferase, Alanine aminotransferase and Alkaline phosphatase), kidney function (Blood urea nitrogen, uric acid and serum creatinine), serum
Summary
38
glucose and lactate dehydrogenasewere carried out by using commercially available kits provided by Human Diagnostics, Germany.
Results:The present study revealed that Moringa oleifera leaf powder supplementation during heat stress has a significant effect (P<0.05) on serum redox status. Serum malondialdehyde and catalase were significantly reduced by Moringa oleifera leaf powder supplementation. On the other hand, Moringa oleifera leaf powder demonstrated a non-significant effect (P>0.05) on hepatic and kidney function. Serum Glucose was also not altered by powdered leaf supplementation of Moringa oleifera.
Statistical analysis: The data was analyzed by using one way analysis of variance (ANOVA) using SPSS software. The group differences were compared by the Tukey’s Test. Differences were considered significant at P<0.05.
Conclusion: From this study, it was concluded that Moringa oleifera leaf powder (MOLP) has a potent antioxidant potential without any marked ameliorative or deteriorative effect on hepatic and kidney functioning in heat stressed rats. Further studies are required to explore the potential of other portions of this plant in heat stress. Availability: Items available for loan: UVAS Library [Call number: 2783-T] (1).
