1.
Prebiotics Production Using Recombinant B-Galactosidase And Their Effect On Growth Of Rat Colon
by Mubbashir Hassan Khan | Sr. Sanaullah Iqbal | Dr. Muhammad | Dr. Naureen Naeem.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: In this research work the prebiotic was prepared by using the enzyme from Kluyveromyces lactis and different doses were fed to the rats to check the growth of lactobacillus, bifidobacterium and E. coli. Firstly the enzyme activity was checked at different concentrations at different time intervals by using 20g/l lactose solution. The glucose, galactose and lactose and their mixture were used as a standard in the same concentration. After many thin layer chromatography analysis the 10µl enzyme for 45 min was enough to convert 1ml of the lactose solution.
Then, by using the same concentrations of the enzyme prebiotics galactooligosaccharides were synthesized by transgalactosylation and were fed orally to group 1 for 10 days, group 2 for 20 days and group 3 for 30 days at concentrations of 1g/day, 2g/day and 3g/day respectively. After 10, 20 and 30 days the fecal samples from all the groups were collected aseptically to check the growth of lactobacillus, bifidobacterium and E. coli. Colony counting was done to count the colonies.
In the study different doses of prebiotics GOS were given @ 1g/day, 2 g/day and 3 g/day. The plates were incubated at 37 ºC and numbers of colonies were counted on digital colony counter. The effect of different concentrations of dose of galactooligosaccharides and the effect of time duration was calculated. The P-value used was 0.05. The P-value obtained by the two way ANOVA for the effect of dose of different concentrations is 0.354521 which is more than 0.05. This value indicates that the effect of dose on lactobacillus growth is insignificant as for as the concentration is concerned. As the concentration is increased there is no effect on growth. While the other factor was the time duration i. e 10, 20 and 30 days so its effect is calculated. P-value obtained for the effect of time is 0.029958. It shows that the result is significant. As the time interval is increased the CFU/ml of lactobacillus also increased.
The consequence of different concentration of dosage of prebiotic galactooligosaccharides (GOS) and the effect of time period is determined. 0.05 P-value used. The P-value deducted by using two-way ANOVA for the effect of dose of different concentrations is 0.032328 which is less than 0.05. This value indicates that the effect of dose on bifidobacteria growth is significant. As the concentration is increased there is increasing effect on growth. While the other factor was the time duration i. e 10, 20 and 30 days so its effect is determined. P-value obtained for the effect of time is 0.014286. It shows that the result is significant. As the time interval is increased the CFU/ml of bifidobacteria also increased. The consequence of different concentration of dosage of prebiotic galactooligosaccharides (GOS) and the effect of time period is determined for E. coli. The P-value obtained 0.03984 which is less than 0.05. This value indicates that the effect of dose on E. coli growth is significant. As the concentration is increased there is increasing effect on growth. P-value obtained for the effect of time is 0.004848. It shows that the result is significant. As the time interval is increased the CFU/ml of bifidobacteria also increased.
Availability: Items available for loan: UVAS Library [Call number: 1833,T] (1).
2.
Production Of Inulin And Fructo-Oligosaccharide Mixture And Evaluation Of It In Vitro Prebiotic Properties
by Pakeeza rafiq | Dr. Sanaullah iqbal | Dr. Naureen naeem | Prof. Dr.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2029,T] (1).
3.
In Vitro Antibacterial Activity Of Star Anise (Illicium Verum) Oil Against Common Food Borne Pathogens And Its Utilization in Cookies Preparation
by Shamim khalid | Dr. Naureen naeem | Dr. Sanaullah iqbal | Prof. Dr. Aftab.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2033,T] (1).
4.
Growth Inhibiting Effects Of Cinnamon (Cinnamomum Verum) Oil On Common Food-Borne Pathogens And Its Utilization
by Sidrah naseer | Dr. Naureen naeem | Dr. Sanaullah iqbal | Prof .Dr. Aftab.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2034,T] (1).
5.
The Effect Of Supplementation Of Galacto-Oligosaccharide And Fructo-Oligosaccharide + Inulin On Mineral Absorption in Rats
by Kaleemullah maawia | Dr. Sanaullah iqbal | Dr. Naureen naeem | Prof. Dr. M.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2067,T] (1).
6.
Microbial Quality Of Fresh And Frozen Fish From Markets Of Lahore
by Irfan iqbal khan | Dr. Naureen naeem | Dr. Muhammad | Ms. Farasat rizwan.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2068,T] (1).
7.
Detection Of Bacterial Load In Quail Meat Available In Lahore Market
by Muhammad Rameez akram | Dr. Naureen naeem | Ms. farasat rizwan | Prof. Dr. Aftab.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2078,T] (1).
8.
Effect Of Prebiotic Galacto-Oligosaccharides On Serum Lipid Profile In Female Rata Fed On Hypercholesterolemic Diet
by Arooj Hashmi | Dr. Naureen Naeem | Dr. Sanaullah Iqbal | Prof.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2091,T] (1).
9.
Preparation Of Galacto-Oligosaccharides In Lactose And Their Influence On Various Biomarkers Of Colon Carcinogenesis In Male Rats
by Fatima Syed | Dr Naureen Naeem | Dr. Sanaullah Iqbql | Prof. Dr. M.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2092,T] (1).
10.
The Effect Of Chemical Treatment During Washing On Reduction Of Aflatoxins In Red Chillies
by Zubair Arshad | Dr. Naureen Naeem | Dr. Muhammad | Dr. Sanaullah Iqbal.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2093,T] (1).
11.
A Comparative Study Of The Effect Of Traditional And Commercial Packaging On The Storabilityu Of Wheat Flour
by Tariq Shahzad Jutt | Dr. Zubair Farooq | Dr. Mateen | Dr. Naureen Naeem.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2113,T] (1).
12.
Assessment Of Microbial Load In Vegetables Grown In Sewage Polluted Water In Lahore Surroundings
by Asifa | Dr. Sanaullah Iqbal | Dr. Naureen Naeem | Prof. Dr. Aftab.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2119,T] (1).
