101.
Prevalence And Chemotherapy Of Mites Infestation In Sheep In Tehsil Bhag Of District Bolan
by Shujat Ali (2008-VA-208) | Prof. Dr. Kamran Ashraf | Dr. Nisar Ahmed | Dr. Muhammad Avais.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Estimated population of sheep in Pakistan is 27.8 million. Balochistan is the largest province which comprises 44 percent of the total area of Pakistan and only 4.9% of entire population of the country. Share of Livestock in Agriculture is 55%, 11.4% of National GDP of Pakistan and more than 47% in the economy of Balochistan. In between the Chelicerates, (mites and ticks) characterize the biggest and most wide taxon, with a valued 0.5–1 million species. More then 48,000 species defined (Halliday et al., 2000). Mange is a contagious disease showing signs like crusty, dermatitis and loss of hairs. Almost 50 mites species having 16 families and 26 genera responsible for mange where all the main mite species having the orders of Astigmata and Prostigmata. Bolan district is situated in the center of Balochistan province of Pakistan Population estimate 640,000 (2005). Bolan district is administratively subdivided into six tehsils viz Bhag, Dhadar, Machh, Sani, Khattan. The present study was carried out in tehsil Bhag. Latitude 29.0415, longitude 67.8239, Altitude 88 meters above the sea level. 1442 square kilometer of Tehsil Bhag. Mean rainfall is 209.9 mm, range of temperature (Avg) is between 40.6°C and 14.58°C. Four distinct breeds of sheep found in Balochistan are Balochi, Bibrik, Harnai, Rakhshani.
A total of 200 sheep were randomly selected to study the prevalence of mites’ infestation. Skin scraping technique was used. For chemotherapy 30 sheep positive for mange mites through skin scraping test were randomly selected and divided into 3 groups of viz A, B, C. Each group contain 10 number of sheep. Sheep’s in group A were injected Ivermectin at 0.2mg/kg bwt subcut while the animals in group B, were treated with Trichloroforn in the form of 0.15% solution as topical application. The members in group C were treated topicaly with aqueous
Summary
37
extract of Nicotiana Tobacum (tobacco). Treatment were done on day zero and repeated on day 15. The sheep in each group were examined in routinely and samples of skin scraping were collected at day 0, 7, 14 and 28 days (Habib et al., 2009). The effectiveness of particular treatment was estimated on the basis of reduction of clinical sign and negative skin scraping.
200 sheep of different breed, age, sex and areas were examined. 30/200 (15%) sheep were found positive for mange mites infestation. Mites infestation was noticed high in male sheep (16%) as compare to female sheep (14%). According to Breed Balochi sheep breed was noticed highly positive (22%) for mange mites infestation. Area wise prevalence was witnessed high in union council Bhag (25%). Mostly effective drug observed for mites infestation was Ivermectin with 90% efficacy at day 28 in conclusion mange mites infestation in sheep at Bhag tehsil of Dist Bolan and Ivermectin is the best effective drug for mange mites into the following in order by Seguvan and Nicotiana Tobaccum. Availability: Items available for loan: UVAS Library [Call number: 2676-T] (1).
102.
Epidemiology And Control Of Gastro-Intestinal Nematodes Of Large Ruminants In Balochistan
by Muhammad Ramzan (2009-VA-653) | Dr. Nisar Ahmad | Prof. Dr. Muhammad Azam Kakar | Prof. Dr. Kamran Ashraf | Prof. Dr. Aneela Khurram.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: The main area of research in this study was to assess the prevalence, hematological aspects
of Bovine nematodiasis. Three main experiments were conducted to highlight the objectives of
the present research study.
The first experiment was conducted to find out the prevalence of large ruminants major
nematodes for one year. For this purpose buffalo and cattle of either sexes and between < 1 year
to > 2 years of age were selected from two sites i.e., Quetta and Qilla Abdullah. Fecal analysis of
these cattle and buffalo showed overall higher (33.99%) nematodes prevalence recorded in buffalo
in Quetta, (27.99%) in cattle at Qilla Abdullah followed by in cattle at Quetta (26.66%). Five
nematode infection was recorded in all two experimental sites with higher prevalence of
Haemonchus contortus in buffalo at Quetta and Ostertgia ostertagi in cattle at Quetta and Qilla
Abdullah. The buffalo and cattle of < 1 year presented higher nematodes prevalence than 1-2 years
and > 2 years. The female buffalo and cattle were infected with nematodes prevalence higher
than male animals. These five nematodes were prevalent almost throughout the year, however a
peak infection was recorded during August and September in cattle and October in buffalo. The
high temperature, rainfall and humidity during these months may be predisposing factor of higher
prevalence. Mostly the level of nematodes infection was low(< 800 EPG) and did not seriously
impaired the buffalo and cattle productivity.
Second experiment on assessing the comparative efficacy of anthelmintics (Levamisole,
Oxafendazole and Ivermectin) against cattle and buffalo nematodes were conducted at Govt and
private farms. The results showed that Ivermectin than Oxfendazole were found effective against
cattle and buffalo nematodes. The higher (89-100%) reduction of EPG were recorded in cattle and
87
buffalo calves treated with Ivermectin followed by Oxfendazole (86-100%), Levmisole (88-
100%).
Third experiment was conducted to determine the hematological values in healthy and
nematodes infected animals. Different hematological parameters i.e., TEC, TLC, Hb estimation,
were determined. The results showed that overall low Hemoglobin estimation and RBC were
recorded in nematodes infected animals than healthy, while higher WBC were recorded in
nematodes infected animals than healthy. The Lymphocytes and Neutrophil and Monocytes were
higher in some nematodes and lower in other, while higher mean Eosinophil counts was recorded
in all nematodes infected animals than healthy animals. Availability: Items available for loan: UVAS Library [Call number: 2730-T] (1).
103.
Evaluation Of Cardioprotective Effect Of Citric Acid On Serum Biochemical Profile Against Isoproterenol Induced Myocardial Infarction In Rabbits
by Aasma Shabbir (2014-VA-525) | Prof. Dr. Habib ur Rehman | Dr. Muhammad Shahbaz Yousaf | Dr. Nisar Ahmad .
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Isoproterenol is a drug which is used to treat heart attack, congestive heart failure, shock and certain types of irregular heartbeat. In addition to this, it is also employed during the process of anesthesia to avoid the constriction of airways. Isoproterenol is a synthetic catecholamine which produced myocardial infarction because of production of cytotoxic free radicals. Citric acid is water soluble and is most important antioxidant and enzyme cofactor. Recent evidence suggests that citric acid possess antioxidant activity.
The aim of this study was to optimize a supplement at which citric acid can act as cardio protector against isoproterenol and also to evaluate its effect on level of CK-MB, serum glucose, serum creatinine, urea, uric acid, triglycerides, total cholesterol, HDL-C, AST, ALT, ALP.
Forty rabbits were selected and housed in the experimental shed of the Department of physiology, University of Veterinary and Animal Sciences, Lahore. Before the arrival of rabbits, the shed was cleaned and fumigated. The rabbits were divided randomly in to five groups, each with eight replicates (n=8 in each group). Animals were treated by following treatment plan; Group 1: (Negative Control) Animals received normal saline 1ml orally for 14 days. Group 2: (Positive Control) Animals received normal saline 1ml orally for 14 days and then myocardial infarction induced on 15th day. Group 3: Animals received citric acid 250 mg/kg body weight orally (dissolved in 1 ml distill water) for 14 days and then myocardial infarction induced on 15th day. Group 4: Animals received citric acid 500 mg/kg bodyweight orally (dissolved in 1 ml distill water) for 14 days and then myocardial infarction induced on 15th day. Group 5: Animals received citric acid 750 mg/kg body weight orally (dissolved in 1 ml distill water) for 14 days and then myocardial infarction induced on 15th day.
At the end of the experiment, rabbits were slaughtered to collect blood samples for serum biochemical analysis (CK-MB, lipid profile, LFT’s, RFT’s, serum glucose). Data was analyzed by one way analysis of variance using SPSS software (SPSS Inc. version 20, Chicago, Illinois). The group differences were studied by using Duncan’s multiple range tests. The P value <0.05 was considered as significant. Data was presented as mean ± SD.
Results showed that the level of CK-MB, creatinine, urea, HDL-C, ALT were found significant (P<0.05) in rabbits compared with the control. While there was no significant effect found on serum glucose, uric acid, total cholesterol, triglycerides, AST, ALP in all the experimental groups compared with control.
From our study we have concluded that supplementation of citric acid has cardioprotective effect against isoproterenol induced myocardial infarction in rabbits. It shows significant effect on CK-MB, HDL-C, ALT, urea and creatinine. While there was no significant effect found on serum glucose, uric acid, total cholesterol, triglyceride, AST, ALP.
Availability: Items available for loan: UVAS Library [Call number: 2703-T] (1).
104.
Comparative Freestall Occupancy Behavior Of Nili Ravi Buffalo And Holstein Cattle
by Sayyad Hussain Magsi | Dr. Muhammad Qamar Shahid | Dr. Nisar Ahmad | Dr. Muhammad Naveed-ul-Haque.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Livestock has great economic importance for our country, with 11.6% share to the total GDP of Pakistan (GOP, 2015-2016). It plays a major role in poverty alleviation in rural area of the country. Buffalo is the major milk producing animal. The share of buffalo milk is 61% of the total milk produced in the country (GOP, 2015-16). Buffaloes are managed under traditional housing where these animals are kept tethered most of the time. The lying surface always remains wet with their urine and feces thereby compromising animal production potential and health. Freestall housing is a better housing option with the specific purpose of providing a comfortable place for dairy cattle to lie down while motivating defecation and urination in the alley outside of the freestall. This system of housing in buffalo can improve their performance and needs to be explored.The current trial was conducted at Dairy Animal Training and Research Center, University of Veterinary and Animal Sciences, Ravi Campus. The comparative freestall occupancy behavior of Nili Ravi buffaloes and Holstein pregnant heifers had been assessed. Thirty Nili Ravi (primiparous and multiparous) pregnant buffaloes and 30 Holstein pregnant heifers was introduced in a naturally ventilated shed having freestall. These animal were given free choice to sit in the sand bedded freestalls, in alleys with concrete floor or in open area with dirt for seven days and their stall occupancy behavior was observed.In second phase, animals restricted under covered area to forcibly (against one’s will) choose between sand bedded cubicles and concrete alley during night. The descriptive statistics was presented in %age of stall usage for every day till maximum stall filling. The logistic regression was used to compare the effect of parity, and weight on stall usage. Kaplan Meier survival curve was estimated to compare stall occupancy between parity.
When buffaloeswere given free choice to lie in freestall, or on concrete floor alleys or to lie on dirt floor in open area, they preferred to lie in open dirt area as compared to freestalls. When
Holstein heifers were given free choice, initially they preferred to lie in open dirt area but gradually the percentage of lying in freestalls increased. Similarly whenBuffaloes and Holstein heifers were restricted under covered area to forcibly choose between sand bedded cubicles and concrete alley during night, Holstein heifers started to lie in cubicles quickly as compared to buffaloes.Holstein heifers prefer to lie in freestalls when given free choice in comparison to buffaloes. Buffaloes having 4th and 3rd parity started to lie in cubicles earlier as compared to buffaloes having 2nd and 1stparty.This study could provide an important bench mark for freestall housing in buffaloes.
Livestock has great economic importance for our country, with 11.6% share to the total GDP of Pakistan (GOP, 2015-2016). It plays a major role in poverty alleviation in rural area of the country. Buffalo is the major milk producing animal. The share of buffalo milk is 61% of the total milk produced in the country (GOP, 2015-16). Buffaloes are managed under traditional housing where these animals are kept tethered most of the time. The lying surface always remains wet with their urine and feces thereby compromising animal production potential and health. Freestall housing is a better housing option with the specific purpose of providing a comfortable place for dairy cattle to lie down while motivating defecation and urination in the alley outside of the freestall. This system of housing in buffalo can improve their performance and needs to be explored.The current trial was conducted at Dairy Animal Training and Research Center, University of Veterinary and Animal Sciences, Ravi Campus. The comparative freestall occupancy behavior of Nili Ravi buffaloes and Holstein pregnant heifers had been assessed. Thirty Nili Ravi (primiparous and multiparous) pregnant buffaloes and 30 Holstein pregnant heifers was introduced in a naturally ventilated shed having freestall. These animal were given free choice to sit in the sand bedded freestalls, in alleys with concrete floor or in open area with dirt for seven days and their stall occupancy behavior was observed.In second phase, animals restricted under covered area to forcibly (against one’s will) choose between sand bedded cubicles and concrete alley during night. The descriptive statistics was presented in %age of stall usage for every day till maximum stall filling. The logistic regression was used to compare the effect of parity, and weight on stall usage. Kaplan Meier survival curve was estimated to compare stall occupancy between parity.
When buffaloeswere given free choice to lie in freestall, or on concrete floor alleys or to lie on dirt floor in open area, they preferred to lie in open dirt area as compared to freestalls. When
Holstein heifers were given free choice, initially they preferred to lie in open dirt area but gradually the percentage of lying in freestalls increased. Similarly whenBuffaloes and Holstein heifers were restricted under covered area to forcibly choose between sand bedded cubicles and concrete alley during night, Holstein heifers started to lie in cubicles quickly as compared to buffaloes.Holstein heifers prefer to lie in freestalls when given free choice in comparison to buffaloes. Buffaloes having 4th and 3rd parity started to lie in cubicles earlier as compared to buffaloes having 2nd and 1stparty.This study could provide an important bench mark for freestall housing in buffaloes.
Availability: Items available for loan: UVAS Library [Call number: 2766-T] (1).
105.
A Clinico-Biochemical Study Of Xylazine-Propofol,Meditomidine-Propofol, Diazepam-Propofol During Ovariohystrectomy Operationin Female Dogs
by Muhammad Ajmal (2006-VA-175) | Dr. Sadaf Aslam | Dr Ayesha Safdar | Dr. Nisar Ahmad.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Background:
Awareness among the small animals practitioner as the impedance has increased many fold on veterinary surgeons to select an ideal anesthetic provoking an ideal outcome during the ovariohystrectomy surgical interventions. The anesthesia should be selected in such a way that animal can recover from anesthesia early on and the operating room time for each patient be minimized in such a way that the animal bears minimum cardiovascular, hepatic, and renal changes due to different pre-anesthetic drugs used.
Hypothesis:
• Medetomidine-propofol might be better among all other three.
Parameters/Methodology:
For this study, fifteen dogs brought to the Pet center for ovariohystrectomywere selected, the
animalswere allocated into three treatment groups, viz. Groups A and Group B and Group C
with 5 animals each.
In group A animalsXylazineHCL was administered intramuscularly at the dose rate of 2.2mg/kgbody weight as pre anesthetic. After 3-5 minutes of preanesthetic injection propofol (6 mg/kg ofbody weight, IV) was injected. In group B, after surgical preparation, Medetomidine HCL in all the five dogs wasinjecting intramuscularly at the dose rate of 40 µg/kg body weight as pre anesthetics. After 3-5 minutes of pre anesthetic injection propofol (6 mg/kg of body weight, IV) was injected.
The dogs of group C Diazepam was injected intramuscularly (0.25 mg/kg) as pre
anesthetic. After 3-5 minutes of pre-anestheticinjectionpropofol (6 mg/kg of body weight, IV)
was injected.
Whilst regularly checking reflexes after short intervalsof time. After achieving of the
surgical stage of anesthesia, the surgical operation was begun.
Biochemical profile assessment was done for renal function tests (serum creatinine,
BUN) and live function tests (ALT, AST, and Alkaline phosphates).
Statistical Design:
The data wasanalyzed by One Way ANOVA followed by multiple comparison tests using
Statistical Package for Social Science (SPSS) version 20.0. The proportionate data was
analyzed through z-distribution test.
Outcome: Considering the significance of anesthesia in surgical interventions, this project was designed tostudy the effect new anesthesia combinations on cardiovascular system, renal and hepaticfunction for an efficacious and ideal pre-anesthetic combination for futuristic surgicalinterventions in dogs, with the aim to minimize surgical complications and accentuate early recovery.
LITERATURE CITED
Adetunji A, Ajadi R, Adewoye C, Oyemakinde B. 2002. Vol. 57 (4) 2002 Total Intravenous
Anaesthesia WithPropofol: Repeat Bolus Versus Continuous Propofol Infusion
Technique InXylazine-Premedicated Dogs.Veterinary Medicine.57 4.
Ambros B, Duke-Novakovski T, Pasloske KS. 2008. Comparison of theanesthetic efficacy and
cardiopulmonary effects of continuous rate infusions of alfaxalone-2-hydroxypropyl-β-
cyclodextrinandpropofol in dogs. American journal of veterinary research. 69 (11):
1391-1398.
Andaluz A, Trasserras O, Garcia F. 2005. Maternal and fetal effects of propofol anaesthesia in
the pregnant ewe. The Veterinary Journal. 170 (1): 77-83.
Borowicz KK, Czuczwar SJ. 2003. Effects ofetomidate, ketamine or propofol, and their
combinations with conventional antiepileptic drugs on amygdala-kindled convulsions in
rats. Neuropharmacology. 45 (3): 315-324.
Cillo JE 1999. Propofolanesthesia for outpatient oral and maxillofacial surgery. Oral Surgery,
Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 87 (5): 530-538.
Cockshott I, Briggs L, Douglas E, White M. 1987. PHARMACOKINETICS OF PROPOFOL IN
FEMALE PATIENTS Studies Using Single Bolus Injections. British Journal of
Anaesthesia. 59 (9): 1103-1110.
Concannon P, Meyers-Wallen V. 1991. Current and proposed methods for contraception and termination of pregnancy in dogs and cats. Journal of the American Veterinary Medical
Association. 198 (7): 1214-1225.
Davidson EB, Payton ME. 2004. Comparison of laparoscopic ovariohysterectomy and
ovariohysterectomy in dogs. Veterinary Surgery. 33 (1): 62-69. 22
Franks N, Lieb W. 1994. Molecular and cellular mechanisms of general anaesthesia.Nature. 367
(6464): 607-614.
Gales NJ 1989. Chemical restraint and anesthesia of pinnipeds: a review. Marine MammalScience. 5 (3): 228-256.
Gonzalez AD, Pechko AH, Kalafsky RE. 2003Topical compositions with a reversible
photochromic ingredient. In: Google Patents.
Hedenqvist P, Hellebrekers LJ. 2003. Laboratory animal analgesia, anesthesia, and euthanasia.
Handbook of Laboratory Animal Science. Volume 1: Essential Principles and Practices.
413-456.
Heidari SM, Loghmani P. 2014. Assessment of the effects of ketamine-fentanyl combination
versus propofol-remifentanil combination for sedation during endoscopic retrograde
cholangiopancreatography. Journal of research in medical sciences: the official journal of
Isfahan University of Medical Sciences. 19 (9): 860.
Jalanka HH, Roeken BO. 1990. The use of medetomidine, medetomidine-ketamine
combinations, and atipamezole in nondomestic mammals: a review. Journal of Zoo and
Wildlife Medicine.259-282.
Kanda T, Hikasa Y. 2008. Neurohormonal and metabolic effects of medetomidine compared
withxylazine in healthy cats. Canadian Journal of Veterinary Research. 72 (3): 278-286.
Kawai S, Takagi Y, Kaneko S, Kurosawa T. 2011. Effect of three types of mixed anesthetic
agents alternate to ketamine in mice. Experimental Animals. 60 (5): 481-487.
Kotani Y, Shimazawa M, Yoshimura S, Iwama T, Hara H. 2008. The experimental and clinical
pharmacology of propofol, an anesthetic agent with neuroprotective properties. CNS
neuroscience& therapeutics. 14 (2): 95-106. 23
Lee DC. Using silver spike point needle-free electro-acupuncture for postoperative pain
management. Victoria University; 2008.
Maddern K, Adams VJ, Hill NA, Leece EA. 2010. Alfaxalone induction dose following
administration of medetomidine and butorphanol in the dog. Veterinary Anaesthesia and
Analgesia. 37 (1): 7-13.
Marik PE 2004. Propofol: therapeutic indications and side-effects. Current pharmaceutical
design. 10 (29): 3639-3649.
Mielck F, Stephan H, Weyland A, Sonntag H. 1999. Effects of one minimum alveolar anesthetic
concentration sevoflurane on cerebral metabolism, blood flow, and CO2 reactivity in
cardiac patients. Anesthesia& Analgesia. 89 (2): 364-369.
Mohammad FK, Faris GA-M, Al-Zubeady AZ. 2012. Developmental and behavioral effects of
medetomidine following in ovo injection in chicks. Neurotoxicologyand teratology. 34
(1): 214-218.
Muir III WW, Wiese AJ, March PA. 2003. Effects of morphine, lidocaine, ketamine, and
morphine-lidocaine-ketamine drug combination on minimum alveolar concentration in
dogs anesthetized with isoflurane. American journal of veterinary research. 64 (9): 1155-
1160.
Prassinos NN, Galatos AD, Raptopoulos D. 2005. A comparison of propofol, thiopental or ketamine as induction agents in goats. Veterinary Anaesthesia and Analgesia. 32 (5): 289-296.
RANA MS. GENERAL ANAESTHESIA IN SWINE: COMPARATIVE EFFICACY OF
PROPOFOL, KETAMINE AND THIOPENTONE WITH XYLAZINE
PREMEDICATION. 2013. 24
Rodrigues SF, de Oliveira MA, Martins JO, Sannomiya P, de CássiaTostes R, Nigro D,
Carvalho MHC, Fortes ZB. 2006. Differential effects of chloral hydrate-and
ketamine/xylazine-inducedanesthesia by the sc route. Life sciences. 79 (17): 1630-1637.
Sams L, Braun C, Allman D, Hofmeister E. 2008. A comparison of the effects of propofol and
etomidate on the induction of anesthesia and on cardiopulmonary parameters in dogs.
Veterinary Anaesthesia and Analgesia. 35 (6): 488-494.
Suarez MA, Dzikiti BT, Stegmann FG, Hartman M. 2012. Comparison ofalfaxalone and
propofol administered as total intravenous anaesthesia for ovariohysterectomy in dogs.
Veterinary Anaesthesia and Analgesia. 39 (3): 236-244.
Tusell J, Andaluz A, Prandi D, Costa C, Garcıa F. 2005. Effects of epidural anaesthesia–
analgesia on intravenous anaesthesia with propofol. The Veterinary Journal. 169 (1): 108-
112.
Uilenreef JJ, Murrell JC, McKusick BC, Hellebrekers LJ. 2008. Dexmedetomidine continuous
rate infusion during isoflurane anaesthesia in canine surgical patients. Veterinary
Anaesthesia and Analgesia. 35 (1): 1-12.
Wheaton L, Benson G, Tranquilli W, Thurmon J. 1989. The oxytocic effect of xylazineon the
canine uterus. Theriogenology. 31 (4): 911-915.
Availability: Items available for loan: UVAS Library [Call number: 2771-T] (1).
106.
Effect Of Drinking Water Quality On Growth Performance Of Post Weaned Holstein Friesian Calves
by Hina Tahir (2006-VA-119) | Dr. Nisar Ahmad | Dr. Jalees Ahmad Bhatti | Dr. Saeed Ahmad.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Information on production potential of Holstein Friesian calves raised under various
sources of drinking water with different levels of TDS as well as its adaptability has not been
documented yet in central Punjab. In this regard, this study was designed to evaluate the effect of
drinking water sources on physiological and production parameters in Holstein Friesian calves.
Holstein Friesian calves exposed to increasing level of TDS water conditions at dairy
farms may exhibit variation in water intake and reduce dry matter intake that might ultimately
lead to deviation in growth performance from normal range. In order to test this hypothesis, the
proposed study was carried out.
To evaluate the physiological and productive profile of Holstein Friesian calves subjected
to various sources of drinking water with different levels of TDS and minerals concentration
under subtropical conditions, the proposed experiment was conducted at Dairy Training and
Research Demonstration Farm Ravi Campus B Block, Pattoki, University of Veterinary and
Animal Sciences, Lahore. For this purpose, 18 Holstein Friesian calves of the similar age and
weight were randomly selected from the herd maintained at Dairy Training and Research
Demonstration Farm and were divided into three groups i.e. A, B and C with 6 Holstein Friesian
calves in each group. The calves from group A were provided turbine water for drinking
purpose.
CHAPTER 6
SUMMARY
Summary
32
Similarly, calves from group B and C were subjected to normal tap water and processed water
from reverse osmosis unit, respectively. The availability of water was made possible for 24 hours
in front of animals. The water was provided under the shed to these calves. The water troughs
were kept neat and clean during whole of the experimental period. The calves were given free
approach to water trough. The water from three sources were supplied to study the comparative
performance of calves. The data on water intake and dry matter intake were observed daily.
Whereas, weight gain was calculated on fortnightly basis. Feed digestibility was done at end of
trial. Blood mineral profile sampling was done at start and end of trial, all serum samples were
analyzed by spectrometer. The data thus collected were analyzed using analysis of variance
(ANOVA) techniques through (SAS, 2004). Difference among treatment means will be tested
through least significance difference.
The results of experiment were helpful to determine the appropriate water quality (1632.0
TDS to 172 TDS) to enhance the growth of Holstein Friesian calves in central Punjab. This will
also provide useful guideline for safe use of drinking water in the areas having water quality
problems. Calves exposed to low TDS water showed higher water, feed intake and weight gain.
Feed digestibility and blood calcium, phosphorus and magnesium levels were not affected with
increase in salinity of water while ALT values are slightly higher in high TDS water group.
It was concluded that provision of different sources of water with varying levels of TDS
(1632.0 to 172 ppm) had no significant affect on growth of post weaned calves. More research is
to be required to investigate the effect of water quality on growth at different stages of calves for
more prolonged duration. Similar studies are suggested for the indigenous animals for long
Summary
33
period of time. So that a comprehensive findings may be achieved in this regard. It is suggested
that livestock may be offered water of same quality which is consumed by human beings. Availability: Items available for loan: UVAS Library [Call number: 2894-T] (1).
107.
Epidemiology, Molecular Detection, Zoonotic Potential, Heamatology And Chemotherapy Of Cryptosporidiosis In Small Ruminants In Southern Khyber Pakhtunkhwa, Pakistan
by Naimat Ullah Khan (2011-VA-516) | Dr. Muhammad Hassan Saleem | Prof. Dr. Aneela Zameer Durrani | Dr. Nisar Ahmad.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Cryptosporidiosis is one of the most important parasitic enteric protozoan infection affecting all vertebrates. The current study was designed to determine the percent prevalence of cryptosporidiosis in small ruminants and humans along with associated risk factors.
An overall highest percent prevalence of cryptosporidiosis recorded in all four categories of small ruminants were 27.22%, 20.56%, 18.33% and 12.22% in lambs, kids, Sheep and goats respectively.
In the current study, 21.55%, 18.33% and15% prevalence of the Cryptosporidium infection was recorded in sheep in District Kohat, Bannu and Lakki Marwat respectively. In the present study, the highest month wise percent prevalence in sheep, was observed in the month of August (36.66%) followed by April (26.66%), June (26.66%), May, July and September (23.33%), February (10.66%), March (10%), November (10%) while the lowest percent prevalence was observed in the month of December and January (6.66%). In sheep, season wise percent prevalence was also studied where highest prevalence was recorded in summer and autumn season (23.33%), followed by spring (20%) while the lowest percent prevalence was found in the winter season (10%). In sheep, age wise percent prevalence was also studied where highest percent prevalence was found at the age of 1 year (22.38%) followed by 1-2 years (18.03%) while the lowest at the age of 2-3 years (13.46%). In sheep, sex wise percent prevalence was also documented where highest percent prevalence was recorded in female (18.80%) followed by male (17.02%) where lowest percent prevalence was recorded.
In goats, the percent prevalence of the Cryptosporidium infection was also studied in three selected areas where recorded 6.66%, 11.66% and 18.3% prevalence in District Bannu, Lakki Marwat and Kohat respectively. Similarly, in goats, overall highest month wise percent
Summary
154
prevalence was recorded in the month of August (30%), followed by July (23.33%), June (20%), May (16.66%), March and September (13.33%), April and November (10%), January, February and October (3.33%) while the lowest percent prevalence was recorded in December (0%). In the current study, the season wise prevalence was also studied in goats where highest percent prevalence was recorded in the summer season (20.83%), followed by spring (13.33%), autumn (11.66%) while the lowest prevalence was observed in winter season (3.33%). The highest age wise percent prevalence was recorded at the age of 1 year (18.58%) followed by 1-2 years (10.20%) while the lowest at the age of 2-3 years or above (5.95%). According to the sex wise percent prevalence, the highest percent prevalence was recorded in male (12.30%) while the lowest in females (12.17%).
The overall highest percent prevalence of the Cryptosporidium infection was also recorded in lambs in three areas where 33.33%, 25% and 23.33% prevalence was recorded in Kohat, Lakki Marwat and Bannu respectively. The highest month wise percent prevalence was recorded in the month of August (46.6%), followed by other months of the year such as July (40%), April, May and June (30%), September and October (26.66%), November and January (20%) while the lowest in the month of February and December (16.66%) in lambs. The Season wise percent prevalence was recorded in lambs where highest percent prevalence was recorded in summer season (36.66 %), followed by spring and autumn (26.66%) while the lowest in winter season (18.33%). According to the age wise percent prevalence in lambs, the highest prevalence was recorded at the age of 1-15 days (38.09%) followed by 16-30 days (29.41%) while the lowest at the age of 31-60 days or above (15.15%). In lambs, the highest sex wise percent prevalence was recorded in females (31.18%) while the lowest percent prevalence was observed in males (22.98%).
Summary
155
In kids, overall highest percent prevalence was 20.55% recorded in three selected districts where the highest prevalence was recorded in District Kohat (23.33%), followed by District Bannu (20%) while the lowest in District Lakki Marwat (18.33%). In kids the month wise percent prevalence was also studied where the highest percent prevalence was recorded in May and August (33.33%), followed by June, July and September (26.66%), March, April and October (20%), November and December (13.33%) while the lowest percent prevalence was recorded in the month of the January (6.66%). The Season wise percent prevalence was also recorded in kids, where the highest percent prevalence was observed in the summer season (30%), followed by autumn (23.33%), spring (20%) while the lowest prevalence was recorded in winter season (10%). The highest age wise percent prevalence in kids was also recorded at the age of ≤1-15 days (33.92%), followed by 16-30 days (15.38%) while the lowest at the age of ≥31-60 days or above (13.55%). Sex wise percent prevalence was also determined in goat kids where, the highest percent prevalence was recorded in female (20.98%) followed by male kids (19.19%).
To conduct molecular study, 360 fecal samples of sheep were analyzed for presence of the Cryptosporidium oocysts through simple microscopic method first then confirmed by PCR. DNA was extracted with the help of DNA extraction kit (Made in USA, GFC vivantis). The targeted gene of parasite was 18s rRNA which result in amplification of a segment of genomic DNA at 435 bp. The following primers sequence was used for Forward primer: (5-AAGCTCGTAGTTGGATTTCTG- and reverse primers (5-TAAGGTGCTGAAGGAGTAAGG-3. An overall molecular percent prevalence of the Cryptosporidium infection was 24.99% in sheep in three selected zones of southern KPK. The highest molecular percent prevalence was 31.66%, 25% and 18.33% in District Kohat, Bannu and Lakki Marwat respectively.
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The highest season wise molecular percent prevalence was also recorded where the highest percent prevalence was recorded in the summer (33.33%), followed by autumn (30%), spring (26.66%) while the lowest in the winter season (13.33%). Molecular percent prevalence was higher in females (27.08%) than male (25.53%). On the basis of environmental factors, overall the highest percent prevalence was recorded in the month of August where highest ambient temperature, relative humidity and heavy rain fall was recorded.
To find out Zoonotic aspect of the Cryptosporidium infection, the overall highest percent prevalence was recorded in children (16.66%), followed by adults (5.55%).
The highest percent prevalence was recorded in diarrhoeic children where direct contact with small ruminants was observed while the lowest prevalence was recorded in those children where no direct or indirect contact was observed.
To conduct the therapeutic trials, a total of 50 goats were selected of the same weight and age that were naturally infected by Cryptosporidium under field conditions. All the goats were placed under same feeding and management conditions and randomly divided into five groups such as A, B, C, D and E. All animals in groups A, B, C and D were treated with Azithromycin (10mg/kg b.wt), Metronidazole (50mg/Kg b.wt), Allium sativum (50mg/Kg b.wt) and Paromomycin (100mg/kg b.wt) respectively while Group-E was placed as a positive control group. The highest percent efficacy in reduction of OPG was shown by different drugs such as Paromomycine (91.77%) followed by Metronidazole (78.20%), Allium sativum (77.00%) while the lowest percent efficacy was shown by Azithromycin (59.29%). On the basis of hematological study, lower lymphocytes count was (48.39%) recorded in non-infected animals while higher (54.33%) count was recorded in infected animals. Similarly higher eosinophil count was (6.73%) recorded in infected group while lower (50 %) counts were recorded in non-
Summary
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infected group. Hb level was higher in infected group than healthy animals. PCV level was higher (42.94%) in infected animals while low (34.62%) in healthy animals. Biochemical analysis of the serum showed, higher quantity of total protein, albumin, ALP, Sodium, Potassium, Chloride, Zinc, Copper, Urea and Creatinine was recorded in infected goats while lower quantity was observed in healthy goats. Availability: Items available for loan: UVAS Library [Call number: 2880-T] (1).
108.
Larvicidal And Adulticidal Effect Of Natural Herbs Against Mosquito Population
by Anam Shahwar (2015-VA-1347) | Dr. Nisar Ahmad | Dr. Muhammad Imran Rashid | Dr. Uzma Farid Durrani .
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Mosquitoes serve as vectors for a wide assortment of human and veterinary pathogens and cause pervasion of numerous infection. Many processes fall for the control of mosquito but the cross resistance didn’t let them successful. Plants are viewed as the natural industry of such chemicals which have promising medicinal and pesticidal properties. Ocimum basilicum regularly known as Basal and Calotropis procera which is famous as apple of Sodom has shown the insecticidal activities against mosquito. Ocimum basilicum and Calotropis procera has adulticidal and larvicidal activity against laboratory reared mosquito population. For the study plants of Calotropis procera and Ocimum basilicum has collected from Lahore city. Leaves stem and flower of Ocimum basilicum and leaves of Calotrops procera had ground after drying for their soxhelet extraction. Their methanol and aequeous extract was used against laboratory reared mosquito to check their efficacy. Serial dilutions of each plant was given to third and fourth instar larvae and three day old adult. Third and fourth instar larvae has shown complete mortality within one hour in 700ppm and 650 ppm of methanol extract of both plant whereas in water extract the concenteration were 450ppm and 500ppm. Mortality of larvae and adult mosquito has been recorded after every ten minutes for one hour and then after 24 hours. The lethal dose concentration from dose-probit model is 700ppm of and 650ppm (methanol extraction) and 450ppm and 500ppm (water extraction) of Ocimum basilicum and Calotropis procera. according The current study has checked the efficacy of indigenous plant extracts against mosquito for eco-friendly approach to control mosquito. Availability: Items available for loan: UVAS Library [Call number: 2949-T] (1).
