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Isolation And Characterization Of Collagen Type Ii From Poultry Trachea

by Sidra Ashraf | Dr. Abu Saeed Hashmi | Dr. Sualeha Riffat | Zahid Mushtaq.

Material type: book Book; Format: print Publisher: 2011Dissertation note: This project was designed to use poultry waste to isolate and characterize collagen type II from its trachea. Collagen type II is being used along with condroitin sulfate and glucosamine for the treatment of osteoarthritis and is also available as a neutraceutical product in the market. For project purpose, trachea of slaughtered broiler birds were collected from the market and after removing adhering tissue and debris, it was then washed thoroughly first with distilled water and then with deionized water. Tracheal cartilage was then cut into small pieces and defattened with chloroform: methanol (2: 1 v/v) solution. After this, the cut pieces were properly cleaned with deionized water. 0.5% Pepsin solution in 0.5 M acetic acid was prepared. Cartilage was then hydrolyzed by the already prepared 0.5 % pepsin (in 0.5 M acetic acid) at 4 ° C for 48 hours. The extract was then separated from the tracheal pieces and the viscous solution obtained was centrifuged at 12000 rpm for 1 hr at 4 "c. Now the collagen was expected to be in the supernatant which was salted out by adding NaCI to a final concentration of 2.5M and kept for almost 12-16 hrs. This collagen was again centrifuged at 12000 rpm for 1 hr at 4 C. The obtained collagen pallet was redissolved in 0.5 M acetic acid and then it was dialyzed against 0.1 M acetic acid followed by dialysis with distilled water. The sample after dialysis was put in petri dishes and kept in freezer for overnight to let it be prepared for lyophilization. The frozen collagen sample was then lyophilized. After lyophilization, the sample gave an appearance of a white mesh. This sample was reconstituted in PBS with pH 8 to run it on SDS-PAGE. The procedure of SDS-PAGE in non reducing conditions was adopted for the characterization of collagen type II in the sample. The description of results of SDS-PAGE is given below: Lane M contains protein markers of different molecular weight. Lane 1, 2 and 3 contains samples at different steps of the whole procedure showing clear bands of collagen type II. Lane 4 contains lyophilized sample of collagen type II showing the thickest band (alpha chain of collagen type II). In this research, poultry waste has been used for making health improving product. As in our country poultry is used in bulk quantity so if its waste might be used in any medicinal product then it might not only be useful but also economical for such a developing country as ours. Another thing is that as this collagen Type II has been extracted from poultry trachea, it shows that tracheal cartilage is a rich source of such collagen type. Collagen Type II is used in the cure of arthritis especially rheumatoid arthritis so through this research, it has been made clear that poultry waste can be utilized in a positive way in medicinal industry and also that collagen Type II acts as an effective neutraceutical. Availability: Items available for loan: UVAS Library [Call number: 1330,T] (1).

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Assesment Of Postprandial Glycemic Response In Healthy Human With Respect To Some Promising Indigenous Mango Varieties Of Pakistan

by Afrah Jabeen (2014-VA-911) | Mr. Haroon Jamshaid Qazi | Dr. Sanaullah Iqbal | Dr. Sualeha Riffat.

Material type: book Book; Literary form: not fiction Publisher: 2016Dissertation note: Carbohydrates intake result in elevation of BGL. GI is a simple tool to select right carbohydrate foods while GL determines the overall effect of that food on human health. High GI/GL diets are directly associated with progression of diabetes type 2. Mango is ranked among medium glycemic index fruit with GI (51-55) and nutritionally it is rich in dietary fiber. Different mango types have different nutritional composition so it was very necessary to estimate postprandial glycemic response of various mango types to determine the possible positive impact of all varieties on overall human health. Samples of six mango varieties were evaluated for various physico-chemical testing. About 50-gram available carbohydrate from each source i.e. glucose and six mango cultivars were provided to each study person. Later on, post prandial blood glucose of all 10 individuals with a time interval of 15 minutes was determined by finger-prick method up to 120 minutes. ANOVA was used to analyze all assessed attributes statistically. Means were compared through Duncan’s multiple range test for significance. Significance level was defined as p≤0.0.5 SPSS version 20 was used for all statistical analysis. Desi variety among all studied mango types reflected low GI and GL value. Availability: Items available for loan: UVAS Library [Call number: 2697-T] (1).

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