1.
Detection Of Rabies Virus In Population Of Pakistan Bat
by Farzana Shaheen | Dr.Tahir Yaqub | Dr.Zafar-ul-ahsan Qureshi | Prof.Dr.Masroor.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2011Dissertation note: Rabies is a zoonotic disease of the warm blooded animals caused by an enveloped, negative sense RNA virus which is a member of the Lyssavirus genus, belongs to the family Rhabdoviridae. The virus is present in the saliva of the carrier animal (dogs, cats, skunks, foxes, raccoons, bats, groundhogs, farm livestock including cows, sheep, goats, horses and pigs etc). Bats are belonging to order Chiroptera, having about 1100 species.
One hundred bats were captured using the mist net from different areas of Punjab, Pakistan viz; Bahawalpur, Toba Tek Singh, Lahore, Rawalpindi, Attack and Kasur District with the help of Wildlife and Ecology Department, Ravi campus UVAS, Pattoki. The majority of sites visited were water bodies and bat roost. Besides bats six samples were also collected from suspected dog, cow and mules.
The sample type was oropharyngeal swabs and brain samples. All the samples were analyzed with Mice Inoculation Test (MIT), Indirect Fluorescent Antibody Technique (iFAT) and Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). MIT was performed on all the samples, positive control and suspected samples of dog, cow and mule showed typical signs of rabies. While the mice injected with negative control and the bats samples remained healthy and active till four weeks. Similarly, no fluorescence was observed in case of bat samples and negative control while the positive control and suspected dog, cow and mule samples showed prominent focal areas of apple green color fluorescence. All these samples were further analyzed by RT-PCR and results were comparable with MIT and FAT. Specific bands of 879bp were obtained by the amplification of "intergenic" region using specific set of primers.
The present study showed that rabies virus antigen is not present in following 5 species; Taphozous nudiventris, Scotophilus heathii, Scotoecus pellidus, Pipistrellus pipistrellus and Scotophilus kohlil of Pakistani bat population.
Availability: Items available for loan: UVAS Library [Call number: 1256,T] (1).
2.
Dynamics Of Recombinant Avian Influenza Virus H9-Ha Gene Herpesvirus Of Turkey Vector Vaccine In Chicken
by Shumaila Rani | Prof.Dr.Masood Rabbani | Dr.Tahir Yaqub | Prof.Dr.Masroor Elahi Babar.
Material type: ; Format:
print
Publisher: 2010Dissertation note: Avian influenza (AI) is known to exist for centuries. It's primarily a disease of birds. For prevention and control of H9N2 avian influenza disease, HA gene from Pakistani H9N2 field virus isolate (PK-UDL/01/08 H9N2) cloned into a herpesvirus of turkey (rHVT/AI-H9 having full HA) at Institute for Animal Health, Compton, UK, expressing HA proteins, was given subcutaneously (in the neck). Commercial avian influenza vaccine was given to chicken through intramuscular route.
On termination of the experiment, the chicks from all groups were sacrificed and their visceral organs were collected on chick-wise basis. Besides this, blood was collected for complete blood counts (CBC) and blood chemistry. Results of groups were then compared for any significant difference. The data analyses showed that in complete blood counts, there was no significant difference (p<0.05) of total leukocyte counts of different groups of chickens but heterophil percentages showed variation among different groups.
Analyses of Serum chemistry results showed that glucose and protein concentration in serum varies significantly (p>0.05) among the different groups of birds. However, there was no significant difference of cholesterol levels among the groups of chickens.
For determining the persistence of rHVT/AI-H9 having full HA virus in different visceral organ and in blood samples of Group A, results of the PCR showed the persistence of herpes turkey virus in leukocytes, spleen, liver, lung, kidney and heart. This project helped in evaluating dynamics of recombinant HVT containing avian influenza HA gene from avian influenza H9N2 Pakistani isolate.
In future the study may be continued for further biological characterization by isolating the HVT from different visceral organ in different interval of time and fractionating the serum protein and analyzing the gamma globulin fraction in serum to measuring the humoral immune response against the recombinant vaccine.
Availability: Items available for loan: UVAS Library [Call number: 1276,T] (1).
