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Dna Typing Of Pakistani Cattle Breeds (Tharparkar And Red Sindhi) By Microsatellites

by Amber Azam | Miss Sehrish Firyal | Prof. Dr.Masroor Elahi Babar.

Material type: book Book; Format: print Publisher: 2010Dissertation note: Pakistan has vast population of cattle belonging to different breeds. No study on DNA typing of cattle has been conducted in Pakistan. DNA typing of cattle is necessary to overcome the problems like forensic cases, sale & purchase, individual identity in case of fertilization by more than one male and ownership disputes. Microsatellite markers have been proved as an efficient and powerful tool for breed characterization of cattle. In this study, a panel of microsatellite markers, having high polymorphism information content (PlC) values, was developed. Blood samples were taken from jagular vein of two breeds of cattle (Tharparkar and Red Sindhi). DNA was extracted by Inorganic method. Primers of labeled microsatellite markers were optimized for successful amplification conditions in the Bio-Rad thermocycler. Multiplex PCR was performed, for amplification of these labeled microsatellite markers on 44 samples of cattle. (ienotyping analysis was performed for the PCR products of labeled microsatellite markers on agarose gel and then by the genotyper. These results were analyzed statistically software "POPGENE 3.3 and POWER STAT". Allele frequency, heterozygosity, homozygosity and polymorphism information content (PlC) of all microsatellite markers were calculated. Average hetrozygosity, average observed homozygosity and average polymorphism infonnation content (PlC) value for all alleles was 0.60, 0.40 and 0.91 respectively. Almost all of the microsatellite markers showed significant variations in both Tharparkar and Red Sindhi breeds. Microsatellite "1NRAOO5" showed maximum variation i.e. 19 alleles and microsatellite"INRAO23" showed the least variation among all microsatellite markers i.e. 2 alleles. Genotyping results of microsatellite markers were clearly different for two different breeds showing a distinct genetic distance between Tharparkar and Red Sindhi breeds. Results of this study lead to development of a panel of 19 microsatellite markers which can be used for breed characterization of cattle. This was a preliminary study on two cattle breeds (Tharparkar and Red Sindhi) in Pakistan. This facility can be provided on commercial basis to owners. Moreover this study can become the basis for further research investigations on cattle in Pakistan. Availability: Items available for loan: UVAS Library [Call number: 1165,T] (1).

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The Diagnostic Value Of Tta Codon Substitution In Los Angeles Galactosemia

by Sahr Malik | Dr. Muhammad Imran | Dr. Muhammad | Miss. Sehrish Firyal.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1590,T] (1).

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Pcr (Polymerase Chain Reaction) Test Development And Its Application For The Diagnosis Of Congenital Leptin Deficiency

by Nida Fakhar | Dr. Muhammad Imran | Miss Faiza | Miss. Sehrish Firyal.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1383,T] (1).

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Molecular Characterization Of Antimicrobial Resistance Genes In Salmonella Isolates From Diarrheic Calves

by Hania Zulfiqar | Prof. Dr. Tahir Yaqub | Dr. Abu Saeed | Miss. Sehrish Firyal.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2013Dissertation note: A number of infectious (bacteria, viruses, parasites) and non-infectious factors cause diarrhoea in calves. Salmonella bacteria are gram-negative and belong to the family Enterobacteriaceae. Salmonella infections in calves continue to be a major problem worldwide and are responsible of causing major economical losses. To avoid the consequences of disease caused by Salmonella drugs like pencillin, tetracyclines e.g, are given to cattle but it is observed that Salmonella show resistance against these drugs after certain period of time. Salmonella is the major causative agent of calf diarrhea. The antibiotic genes against tetracycline and ampicillin are present in slamonella isolates from calves which are suffering from diarrhea. Aim of my study was 1) Salmonella isolation and investigation of the antimicrobial resistance gene from diarrheic calves and 2) Molecular analysis of antibiotic resistance gene of isolated salmonella species. For this purpose, salmonella antibiotic resistant isolates against ampicillin and tetracycline were selected. Antibiotic resistant plasmid genes were amplified by PCR. After gel electrophoresis the resulting fragments were sequenced through genetic analyzer. After sequencing all the sequences were viewed in Chromas Lite 2.1.1 , Sample sequences were aligned with the reference sequences obtained from NCBI by using Mega 5.05 software. Alignment results show that there is no Single Nucleotide Polymorphism found in salmonella. Availability: Items available for loan: UVAS Library [Call number: 1744,T] (1).

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