1.
Cholecystoduodenostomy As A Surgical Attempt To Establish Biliary Redirection In Dogs
by Hamid Akbar | Mr. Muhammad Arif Khan | Dr. Muhammad | Dr. Muhammad Athar Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2002Dissertation note: Liver is the largest paranchmatous organ of body. Gall bladder is embedded in lobes of liver and it acts as a store house of bile. Liver pours its secretion into gall bladder which temporarily stores it and transports it to duodenum by bile duct which is a small pathway.
Sometimes this pathway (bile duct) is disturbed due to any of multiple reasons described earlier and bile is not transported. Hence there is an immediate need to develop a new connection to restore flow of bile. Cholecystoduodenostomy is a good technique in such cases to restore bile flow. In this project twelve healthy dogs were selected and divided into 3 groups namely, A, B and C. Group A consisted of 8 dogs in which cholecystoduodenostomy was performed. Group B consisted of 2 dogs, in which only laparotomy was experienced, whereas group C also consisted of 2 dogs acted as control.
Liver function tests were carried out in all the dogs before and after surgery. The enzyme values were increased when tested at 8 weeks post-operatively. The dogs were also monitored for various parameters to check the efficacy of technique.
All the dogs were euthanized at 12th week post-operatively to chck healing at surgical site. The analysis of results has clearly indicated that cholecystoduodenostomy is a safe and sound method in obstructive bile duct problems, and this technique can be used without putting the patient in dilemma.
Availability: Items available for loan: UVAS Library [Call number: 0745,T] (1).
2.
Comparative Efficacy Of Crossed Pinning And Plating For The Repair Of Supracodylar Femoral Fracture In Dogs
by Imtiaz Rabbani | Mr. Muhammad Arif Khan | Mr. Asim Khalid Mahmood | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2002Dissertation note: The supracondylar femoral fracture usually falls into a special type of fracture, which is not treated by ordinary limb splintage devices because of its location near the joint as well as strong distraction of the thigh muscles. hence, emphasis is given to convert the distracting forces into Compressive forces to achieve the desired effects. Different types of internal fixation devices have been tried in the past for the repair of this type of fracture like placement of intramedullary pins, rush pins, intramedullary pins in crossed fashion and the application of the bone plate. The present study was conducted on 12 mongrel dogs divided into two groups of 6 animals each. rfhese groups were designated as group A and group B. Two methods, crossed intramedullary pinning and application of the bone plate were carried out for the repair of supracondylar fernoral fracture in dogs. In group A, the supracondylar region of the femur was surgically exposed, fractured and repaired by crossed intramedullary pinning technique. Whereas in group B the supracondylar region was repaired by the application of bone plate (T-plate).
The dogs of each group were kept for a period of 16 weeks postsurgery. '['he results were obtained on the basis of clinical, radiographic and postmortem evaluation. The results of the study clearly indicated that the use of bone plate is a better fixation device with minimal post-operative complications for the repair of supraconclylar femoral fracture in dogs.
Availability: Items available for loan: UVAS Library [Call number: 0747,T] (1).
3.
Effect Of Collection Frequency On The Semen Quality Of Broiler Breeder
by Amjad Riaz | Prof. Dr. Muhammad Aleem Bhatti | Dr. Talat | Mr. Muhammad Amir Saeed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2002Dissertation note: In future, artificial insemination (AI) in poultry industry has a considerable importance because of the excellent results in fertility and hatchability. Increasing male utilisation in artificial insemination depends upon the optimum use of bids by increasing semen collection. The project was designed to scrutinise the effects of collection interval, testes weight and body weight on semen yield.
A total of ten broiler breeder males (35 weeks of age) were randomly selected. After providing ten-day sexual rest these were trained for semen collection by abdominal message technique. In study I the effects of various frequencies of semen collection on semen characteristics were investigated. All birds being collected at 48hours, 24 hours and 12 hours intervals. In each interval five consecutive collections were examined for motility percentage, semen volume and sperm concentration. Out of ten one cock gave no semen through out the study and 2 birds did not yielded semen at 12 hours interval and were excluded from the study.
No significant difference in collection intervals was established for percentage motility (P> 0.05), but semen collected at 12 hours interval was significantly lower than 24 hours interval in respect of semen volume and lower than 48 hours interval in respect of sperm concentration. Thus at 48 hours and 24 hours intervals we get significantly higher values of number of sperms per collection, number of motile sperms per collection and number of doses per collection than the values collected at 12 hours interval (P < 0.05). But difference for theses values at 48 and 24 hours collections intervals was non significant (P > 0.05).
The total number of semen doses over a six-day period increased linearly and significantly as the frequency of collection increased from once every two day to twice daily. Daily semen collection was found to be more suitable for Al programme at broiler breeder farm.
In study II daily semen output, paired testes weight and body weight were correlated. The semen output was found to be significantly correlated with testis weight (r =0.82) and body weight (r = 0.79), but no significant correlation was found between testis weight and body weight (r = 0.56).
In biometrical examination the weight of left testis was found significantly heavier than right testis but in respect of testicular volume no significant difference was observed between the two sides.
Availability: Items available for loan: UVAS Library [Call number: 0785,T] (1).
4.
Determination Of Metabolizable Energy And Dry Matter Digestibility Of Indigenous Feed Staffs Used In Poultry Rations
by Rana Mudassar Riaz | Prof.Dr.Talat Naseer Pasha | Dr. Farina M | Mr. Muhammad Kamran.
Material type: ; Format:
print
Publisher: 2005Dissertation note: The project was conducted to determine the AME, TME and dry matter digestibility. Five feedstuffs were used for this purpose. Thirty 16- week-old cockerels were used in such a way that five birds were force fed 30g of each feedstuff. Five birds were kept fasted to serve as negative control for determination of metabolic feacal and urinary endogenous excretions. The prefeeding fasting period was 21 hr and excreta were collected over a period of 24 hr. Proximate analysis for moisture, crude protein, ash, crude fibre and nitrogen free extract was carried out. The gross energy of the ingredients and the excreta was measured by using adiabatic bomb calorimeter and AME and TME were worked out.
The observed AME values for maize, rice tips, wheat, sorghum and millet were 3612.74, 3661.62, 3296.85, 3514.92 and 3278.46 Kcal/kg respectively. Rice tips have the highest value and on the other hand millet has the lowest value.
The observed TME values for maize, rice tips, wheat, sorghum and millet were 4059.6, 4095.36, 3738.58, 3965.71 and 3730.62 Kcal/kg
respectively. Rice tips have the highest value followed by maize, sorghum, wheat and millet has the lowest value.
Endogenous energy ranged from 11.53 14 to 12.7099 Kcal with the average of 12.02 KcaI.
Dry matter digestibility was highest for rice tips (77.4 1%) followed by maize (75.64%), sorghum (72.13%), millet (71.88%) and lowest value for wheat (67.26%) due to relatively high content of cellulose, hemicellulose, lignin etc. Rice tips have better digestibility so it should be included at higher percentage in poultry feed.
It was also found that crude fiber has indirect relationship with both AME and TME. While ether extract has direct relationship with gross energy.
While for the comparison with the NRC values it was found that for both AME and TME except wheat, other ingredients have lesser values than reported in NRC and statistically it was found that there was non-significant relationship between the observed and standard mean values.
Availability: Items available for loan: UVAS Library [Call number: 0854,T] (1).
5.
Effect Of Different Dietary Energy & Perfomance Of Broiler Chicks
by Muhammad Azam Chaudhri | Dr Nisar Ahmad | Dr Muhammad Yaqoob Malik | Mr Muhammad.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1989Dissertation note: A research project was undertaken to study the effect of different dietary energy and protein ratios on the performance of broiler chicks. The performance of chicks were studied under the headings of weight gain, feed efficiency, feed consumption, dressing percentage and economics. Four starter rations containing calorie protein ratios of 167, 149, 136 and 123 and four finisher rations containing calorie protein ratios of 199, 178, 159 and 145 were tried on one hundred and twenty day old broiler chicks of both sexes for a period of 50 days. The starter rations were fed up to 28 days of age and remaining 22 days the finisher rations were fed.
The results showed that the starter rations containing calorie protein ratios of 136 and finisher ration containing calorie protein ratio of 159 were best in promoting weight gain, feed utilization and were economical as compared to other calories protein ratios. Different calorie protein ratios did not influence the dressing percentage of broiler chicks.
It was concluded that careful combinations of energy and protein in poultry feed formulations
Availability: Items available for loan: UVAS Library [Call number: 0886,T] (1).
6.
Determination Of Apparent Metabolizable Energy Of Indigenous Vegetable Protein Sources In Poultry
by Hammad Ali | Prof.Dr.Talat Naseer Pasha | Dr. Muhammad | Mr. Muhammad Kamran.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2006Dissertation note: An experiment was conducted to determine the apparent metabolizable energy (AME) and true metabolizable energy (TME) of indigenous vegetable protein sources in cockerels by force feeding method. Six indigenous vegetable protein sources viz. soybean meal, canola meal, rapeseed meal, guar meal, sunflower meal and linseed meal were used. Thirty five 18 weeks old broiler breeder cockerels were used in such a way that five birds were force fed 25 gm of each feedstuff for estimation of AME and TME. Five birds were kept fasted to serve as negative control for determination of metabolic faecal and urinary endogenous energy excretions. The pre-feeding fasting period was 24 hours and excreta was collected over a period of 24 hours. The gross energy of test ingredients and the excreta was measured by using adiabatic oxygen bomb calorimeter. Proximate analysis for moisture, crude protein, ether extract, crude fiber, ash and nitrogen free extract were carried out. All had almost similar dry matter and ether extract values. Sunflower meal and canola meal had less CP% because of presence of sulphur containing amino acids. The observed AME values for soybean meal, canola meal, rapeseed meal, guar meal, sunflower meal and linseed meal were 2556, 1920, 1887, 1847, 1629 and 2061 Kcal/Kg, respectively. Soybean meal had the highest value 2556 Kcal/Kg and sunflower meal had the lowest value 1629 Kcal/Kg. The observed TME values for soybean meal, canola meal, rapeseed meal, guar meal, sunflower meal and linseed meal were 2694, 2053, 2022, 1992, 1764 and 2198 Kcal/Kg, respectively. Soybean meal had the highest value 2694 Kcal/Kg and sunflower meal had the lowest value 1764 Kcal/Kg. Endogenous energy ranged from 2.93 Kcal/gm to 2.95 Kcal/gm with the average of 2.94 Kcal/gm. It was also found that crude fiber has indirect relationship with both AME and TME. While, it has also come to notice that ether extract has direct relationship with gross energy of the feedstuff.
Availability: Items available for loan: UVAS Library [Call number: 0936,T] (1).
7.
Genotyping Of Echinococcus Granulosus And Its Comparative Prevalence In Sheep, Goat And Human
by Muhammad Imran Bhatti | Prf.Dr. Azhar Maqbool | Miss. Sabiqaa Masood | Mr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2007Dissertation note: Hydatidosis is caused by metacestode of the dog worm Echinococcus granulosus. It is a serious problem for both Public health and livestock economy.Echinococcus granulosus has number of genetically distinct strains which are known to differ morphologically and epidemiologically. Out of 1000 sheep and goat examined only 45 Samples of hydatid cysts were collected from different organs i.e. livers, kidneys, lungs and hearts from Lahore abbatoir. Fertility and viability of the cysts was observed microscopically. Genotyping of Echinococcus granulosus was performed through Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP). Seroprevalence of hydatidosis in 50 butchers working in abattoir was also determined by the use of Latex agglutination test (LAT) kit for detection of hydatidosis.
Considerable information is available about genetic variants of E. granulosus around the world. Ten genotypes of E. granulosus have been described, which exhibit a diversity of morphology, development, and host range, as confirmed by various studies. In the Mediterranean area, the Gl or common sheep strain, 02, the buffalo strain 03, and the equine strain 04 have been found in Spain, Italy, Lebanon, and Syria
To date, molecular studies using mainly DNA sequences cytochrome oxidase subunit 1 (COl) and NADH dehydrogenase 1 (ND1) genes have identified ten distinct genotypes (01 -G 10) within E. granulosus. This categorization follows very closely the patterns of strain variation emerging from biological and epidemiological traits.
In this study we perform serum analysis of butchers to detect antibodies against Echinococcus so that the prevalence of Echinococus can be checked, the data available indicated that 14% of butchers population is infected with Echinococus. In order to confirm the starin of Echinococcus in sheep and goat the PCR-RFLP analysis of ND I gene of Echinococus were performed .The data obtained was analysed and it was concluded that the 01 strain of echinococus is prevalent in sheep and goat in Punjab area. It is hoped that the findings of the present study will be helpful for further planning about the control of the disease and correlating the prevalence in sheep, goats and butchers from the zoonotic point of view.
The results demonstrated that PCR-RFLP analysis of samples of patients suspected for Echinococcus is a promising diagnostic method and also confirms the type of Echinococus prevalent in that area and also enables an early direct detection of parasite DNA. This will help to curtail this drastic malady at an early stage and will help to devise the trategy to minimize the losses due to this disease.
Availability: Items available for loan: UVAS Library [Call number: 0967,T] (1).
8.
Epidemiological Patterns Of Brucellosis In Sheep, Goats And Human Beings
by Jafar Pasand Masoumi | Sh. Muhammad Amin | Dr. Rashid | Mr. Muhammad Naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1986Dissertation note: The epidemiological patterns of brucellosis were studied on 1554 serum samples (goats, 500, sheep 532, human being 522) and 1027 milk samples (goats 527, sheep 500) for utilizing this information in effectively controlling or eradicating this disease from livestock and human population in Pakistan.
Various tests used comprised slide test, standard tube agglutination test and milk ring test. The antigen for serum agglutination test and slide test was the Standardised Brucella abortus antigen of Veterinary Research Institute, Lahore. For milk ring test haematoxylin stained Brucella abortus antigen developed at Veterinary Research Institute, Lahore was used. An overall incidence of 3.00%, 1.69% and 0.95% was recorded respectively in goats, sheep and human beings by serum agglutination test. The milk ring test gave an infection rate of 9.10% in goats and 7.6% in sheep. The influence of various epidemiolo- gical factors was confirmed and in goats a higher incidence by serum agglutination test was detected in females (3.65%), Teddy breed (.3.93%) and in animals of 4 years and above age group (9.52%). In sheep a higher incidence was recorded in females (3.10%), non-descript breed (3.18%) and in animals of 4 years and above age group (2%). In human beings a higher incidence was observed in males (1.27%), 20-29 years age group (1.86%) butchers (8.33%) and persons in habit of consuming raw milk
(5.40%).
The findings of the present study have revealed an alarming proportion of the disease incidence in our sheep and goats and confirmed the presence of infection in human being. Thjs calls for an immediate response of the experts, and introduction of appropriate brucellosis control measures both in livestock and human population.
Availability: Items available for loan: UVAS Library [Call number: 1116,T] (1).
9.
Fixation Of Fractures By Stainless Steel Intramedullary Pins And Plates Tereatment Of Tibial Fractures In The Dog
by Dr. Mazhar Iqbal | Zafar ud Din Mian | Altaf Hussain | Mr. Muhammad Tufail Khan | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1978Dissertation note: The study of "Treatnt of TibiaS fractures in the cog" through pinning end plating was undertaken primarily for introducing this branch of orthopaedic surgery here to keep pace with other countries of the erld, besides ascertaining the conp are- tive efficiency of both the aforesaid techniques. A total number of 32 dogs of local breed were selected for this purpose and were divided into eight groups of four animals each, The ani:aals in one group n of the sane sex and aqroximately of uniform age, size and weight, tine group of animals was used at a time, The left tibiae of the animals were broken approximately in the zui&ie with the help of chisel and haaaer under general anaesthesia of Intraval Sodiwa, Half of the fractures were ictnobilized using stainless steel intrameduUary pins and the recaining half by stainless steel bone plates, The aninols were kept under the same environmental conditions and fed on sane ration during the post-operative period, The results indicated that both the methods were just equaUy good as far as iaobilization art callus formation was concerned, However, pinning is prefexrd in view of its being simple ext less laborious both at the time of its introduction and removal after recovery. runhcr,,:e, from experiencing very negligible post-operative cocç; ications, pinning was quite economical as compared to plating,
Availability: Items available for loan: UVAS Library [Call number: 1122,T] (1).
10.
Toxonomical Study Of The Genus Trichuris And Comparative Anthelminitc Efficacy Of Fenbendazole (Panacur) Ad Albendazole (Valbazen) Against Gastro-Intestinal Nematodes in Sheep
by Dr. Muhammad Iftikhar | Mr. Asif Rabbani | Mr. M. Akram | Mr. Muhammad Afzal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1990Dissertation note: Pakistan has a large population of livestock, which plays an important role in the economy of the country. Parasitism is a major problem in our country. Great economic losses have been attributed to the sheep population in our country due to the parasitic infections. The genus Trichuris includes some of the most important nematode species effecting the sheep, therefore a taxonomical study of the genus Trichuris was conducted in the month of September and October, 1989. For this purpose 100 guts (large intestine) of sheep slaughtered at Lahore abattoir were procured and brought to the parasitology laboratory College of Veterinary Sciences, for further investigations. The overall incidence of Trichuris spp. was found to be 74% . The following two species were recorded.
1. Trichuris ovis 66%
2. Trichuris globulosa 18%
Taxonomy of Trichuris species was studied by preparing permanent mounts. The present work was also aimed to see the effect of two commonly available anthelmintics namely fenbendazole (panacur) and albendazole (valbazen), on naturally infected sheep with gastrointestinal nematodes. Sixty sheep positive for nematode infections were randomly divided into three groups A,B & C each group comprising of 20 animals. Group A and B
were given fenbendazole and albendazole respectively according to the manufacturers dose rate while group C was kept as untreated control. The efficacy of both the drugs was evaluated on the bases of reduction of eggs per gram of faeces after medication. The egg counts were made on zero, 7th, 14th and 21st day. Both the drugs revealed a progressive decrease in the faecal egg counts. The efficacy of fenbendazole on 7th, .14th and 21st day was 96.33, 97.05 and 99.66% respectively while the efficacy of albendazole was 92.56, 95.37 and 98.18% respectively. There was no significant difference in the effectiveness of the drugs.
Availability: Items available for loan: UVAS Library [Call number: 1130,T] (1).
11.
Studies Of The Carriers Of Pasteurella Multocida
by Syed Shabir Ahmad Shah | Prof. Muhammad yousaf Vaid | Mr. Muhammad | Mr. Muhammad Akram Muneer | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1979Dissertation note: Pasteurellosis is an infectious disease of Livestock having world wide occurrence. To find out the incidence of healthy carriers of Pasteurella multocida in cattle buffaloesa, research project was undertaken at College of Veterinary Sciences, Lahore.
For this purpose 330 (nasopharyngeal) swabs of clinic shy normal cattle and buffaloes were collected from various sources from Lahore.
Inoculations swabs were made on various media like blood agar, tryptore agar, tryptore broth, mutrient agar, nutrient broth, etc. The culture media wee incubated both aerobically and at a temperature of 37oC.
The biochemical characteristics of Pasteurella multocida isolated were studied, smears from growth, were prepared, stained with Gram's method and examined.
Availability: Items available for loan: UVAS Library [Call number: 1136,T] (1).
12.
Epizootiology Of Newcastle Disease In Free Flying Birds Of Pakistan
by Muhammad Arshad | Dr. Muhammad Ajmal | Mr. Mubbasher | Mr. Muhammad Naeem | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1984Dissertation note: Epizoàtiology of Newcastle disease was studied in 105 birds each of three species of free flying birds i.e. pigeon, starling and sparrow. Incidence of Newcastle disease was measured on the basis of Haemagglutination Inhibiting antibodies against Newcastle disease virus present in the serum of each bird. It was observed that 42.86% of pigeon, 25.71% of sparrows and 21.90% of starlings were positive for Newcastle disease. An attempt was also made to isolate the virus from faecal material and pooled organs (lung,liver and spleen) of each bird in developing chick embryo. Three strains of Newcastle disease virus, 2 from pizeon and one from starling were isolated. One pigeon having virus in its organs was also found excreting Newcastle disease virus in its faeces. No virus could be isolated from sparrows. The pathogenicity of isolates was studied in day-old chicks by intracerebral inoculation and it was observed that all of the three isolates were moderately pathogenic Though non significant, the percentage of positive cases on the basis of Haemagglutination Inhibition test in male birds was found to be higher than female birds of the same species
From the tudy it was evident that pigeon could play an important role in the transmission of Newcastle disease. The other two species were also susceptible to this virus and could be source of transmission. It recommended that these as well as other species of the ree flying birds in a large number from much wider areas may be studied. It is further recommended that birds found dead in the field and pet bird markets may be investigated for virus isolation.
Availability: Items available for loan: UVAS Library [Call number: 1143,T] (1).
13.
Molecular Investigation Of Mental Retardation Locus (Mrti)/Gene Prss12 By Linkage Analysis
by Zafar Ali | Prof.Dr.Masroor Elahi Babar | Dr. Aftab | Mr. Muhammad Asif.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2010Dissertation note: Mental retardation (MR) is a condition in which a person having an intelligence quotient (IQ) lowers than 70. It is also associated with a deficit in adaptive behavior such as communication and daily living skills. Mental retardation is either non-syndromic or syndromic. It is one of the most common genetic disorders and it affects about 1-3% of the human population, with a ratio of males higher than females. The present study was can-ied out to determine the prevalence of families having mental retardation in Pakistani population. In the present study, 7 MR families with three or more affected individuals with MR were enrolled. Family history was taken and pedigree was made personally by visiting the families. The blood samples were collected from the enrolled families. Then DNA was extracted from the blood samples collected from these families by standard inorganic protocol. After isolation of DNA from blood samples, 3 STR markers (D4S191, D4S2392 and D4S3024) in vicinity of mental retardation (MR) locus (MRT1)/gene PRSS12 were amplified on each sample of each family by PCR. The amplified PCR product was first checked on agarose gel and then genotyping analysis (linkage analysis) was performed on non denaturing polyacrylamide gel (PAGE). After polyacrylamide gel electrophoresis, picture of the gel was taken and alleles were read manually with larger allele donated by 2 and smaller by 1. After that haplotype was constructed to determined the pattern of inheritance among the affected and normal individuals of each family under study and also to determined that a family was linked or unlinked to mental retardation locus (MRTI)/gene PRSS12. None of the family was linked to mental retardation locus/gene PRSS12. The families which remain unlinked to the reported loci during screening signifies extreme genetic heterogeneity of MR which is not surprising because about 50% of human protein coding genes are expressed in the brain and it provides an excellent resource material for mapping of the new genes which will shed light on the complex pathways involved in the development of learning and memory in those population. The pedigree of each family in the present study showed that most of the marriages are cousin marriages; therefore this study may play a role in creating awareness about the effect of cousin marriages that is the first step towards decreasing socio-economic burden of the country by genetic counseling and also to prevent mental retardation in Pakistan due to inbreeding. Mental retardation locus (MRT1)/gene PRSS12 was studied for linkage analysis in seven families from different areas of District Swat and Peshawar of Khyber Pakhtunkhwa province of Pakistan. None Out of seven families was linked to mental retardation locus (MRT1 )/gene PRSS 12. All the seven families remain unlinked to this locus. It is concluded that Mental retardation is a complex genetic disorder and needs further studies to identify the already known locus or to explore novel loci through genome wide scan responsible for mental retardation in these population. This will provide opportunities of genetic counseling to these populations and will ultimately result in prevention of mental retardation in Pakistani population.
Availability: Items available for loan: UVAS Library [Call number: 1171,T] (1).
14.
The Study Of Gene Gjb2/Dfnb1 Causing Deafness In Humans By Linkage Analysis From District Peshawar
by Noor Badshah | Prof.Dr.Masroor Elahi Babar | Dr. Aftab | Mr. Muhammad Asif.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2010Dissertation note: Hearing impairment is the partial or complete inability to hear that leads to compromise the development of normal language skills. Among all the sensory impairments in humans, hearing impairment is the most common.
It is estimated that at least 50% of the cases are due to genetic factors. Hereditary hearing loss may be syndromic or non-syndromic; about 30% of deafness cases are syndromic, while 70% is non-syndromic. It is estimated that the prevalence of profound bilateral hearing loss is 1.6 per 1000 in Pakistan and 70% of hearing loss arises in consanguineous families. The main pattern of inheritance of deafness in Pakistani population is autosomal recessive and to date more than 145 loci and 26 genes have been identified for non-syndromic recessive deafness.
More than 400 disorders associated with hearing loss shows extreme genetic heterogeneity and complexity of the mammalian inner ear. As more genes are identified, the elucidation of the function of the proteins that these genes encode contributes greatly to the understanding of cochlear mechanisms and their role in disease causation.
The gene involved, GJB2, encodes the connexin26 molecule. Connexin26 is a component of gap junctions, the links that allow small molecules to pass from one cell to the next, and this protein is found in several places in the body, including the epithelial supporting cells surrounding the sensory ear cells of the cochlea.The sensory ear cells of the cochlea allow potassium ions to pass through their upper surface during normal reception of sound, and these potassium ions must be recycled through the base of the ear cells and the supporting cells and fibrocytes back into the high-potassium endolymph that bathes the tops of the ear cells.
The aim of this study was Linkage analysis for DFNB1 locus involved in causing hereditary deafness in families from Khyber Pukhtunkhwa. A total of 10 families were enrolled from different areas of Khyber Pukhtunkhwa province. I have studied 8 families of these 10 (i.e.) family no. 2, 3, 4, 5, 6, 8, 9 and 10. The families have at least three affected individuals. All the families showed recessive mode of inheritance. For linkage analysis studies for DFNB1 locus, three STR markers D13S175, D13S292, and D13S787 were genotyped using Polyacrylamide gel electrophoresis (PAGE) and haplotypes were constructed to determined, linkage with DFNB1 locus. From a total of 8 families, a single family was linked to DFNB1 locus. The DFNB1 locus is the first non-syndromic deafness locus mapped to chromosome 13q12.
Availability: Items available for loan: UVAS Library [Call number: 1191,T] (1).
15.
Dominant Inheritance Of Myopia Linked Loci Myp10 And Myp11
by Saira Nabi | Mr. Asif Nadeem | Mr. Muhammad Imran | Prof.
Material type: ; Format:
print
Publisher: 2010Dissertation note: Myopia, a refractive error is one of the most common ocular disorders worldwide with elongation of axis of the eyeball. Genetic plays an important role in the development of myopia. Familial myopia is common in Pakistan. The aim of the study was to find out the dominant inheritance through linkage analysis and molecular characterization of Myopia in Pakistani myopic families and also to determine the presence of loci (MYP 10 and MYP1 1).
Six families with three or more affected individuals in two or more ioops were enrolled from different areas of Lahore. The persons with refractive error equal or worse than -l were considered as myopic. Out of the total 36 samples, 26 were myopic.
For the each locus, 3 markers were designed .Polyacrylamide Gel electrophoresis (PAGE) was used for genotyping of amplified DNA samples. Haplotypes of all the families were constructed based on the PAGE results to check weather a family is linked or unlinked to those loci. Result of allele haplotyping were analysed to evaluate the linkage of Myopia loci and dominant inheritance in Pakistani families. No linkage was found for any of those selected families. The negative results for these chromosomal regions have several possible reasons; sample size, family size and ethnicity of the families are the major reasons for these negative results. Although there was no linkage for the loci MYP10 and MYP11, this would be the first molecular investigation of the Myopia loci MYP10 and MYP11 in Pakistani families. The findings of the proposed study will be vital for victim familes in terms of genetic testing, genetic counseling, for designing a management plan and resource alloction for victims.
Availability: Items available for loan: UVAS Library [Call number: 1215,T] (1).
16.
Study On Chemical Composition Of Nili Ravi Buffalo Milk
by Yasir Abrar | Prof. Dr. Muhammad Ayaz | Dr. Jaleed | Mr. Muhammad Nadeem.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2010Dissertation note: Milk is a complete food and contains all the nutritional components in balanced form. It helps to meet important nutritional needs of the human body. Vitamins, minerals, sugar, Fat, and protein are present in milk. Among the established breeds of buffalo in Pakistan. the Nili-Ravi breed originated in the valleys of Sutlej and Ravi rivers is dominant in the Punjab province. The purpose of the study is to determine the chemical composition and fatty acid profile of buffalo milk.
The milk animals, Buffaloes were divided into 3 groups on the basis of lactation period i.e early, middle and late lactation. Five animals were present in each group. Six milk samples. 3 in the morning and 3 in the evening from each animal was collected. A total of 90 milk samples were obtained for analysis. Milk in bottles of 500 ml capacity was transported to lab. [he samples were analyzed in Livestock Production Research Laboratory, Livestock Production Research Institute Bahadurnagar (Okara). Temperature, Density, Conductivity and freezing point was determined by using Milkoscan.
Buffalo samples were analyzed for proximate analysis, parameters like fat, crude protein, lactose, ash, solids not fat, total solids, pH and acidity were determined. Fatty acid profile of buffalo milk was determined by the method of AOAC (2000). Milk fat content (%)was Ihund to be significantly (p<0.05) lower in the early (6.52) and mid lactation (6.61) stages than the late lactation stage (6.85). The content of TS (%) was found to be significantly (p<O.05) higher in the late lactation (14.62 and 15.45) stage than the early (12.94 and 13.65) and mid lactation (13.76 and 14.14) stages. In contrast, the content of total protein (4.31 to 4.78%), SNF (X.4() to8.70%), lactose (4.36 to 4.74%), and ash (0.l8to 0.19%) did not vary significantly among the different lactation stages.
Fatty acids profile also checked from Cl to C20. The values of Butyric acid, Caproic acid. Myristic acid, Stearic acid, and Oleic acid showed significant increase towards end of lactation. 'lhcse values of fatty acids also revealed that there is significant difference among the animals and between the lactation stages of Nih- Ravi buffalo milk. Results showed that Capric acid, Laurie acid, Palmitic acid, Palmitoleic acid, Linoleic and Linolenic acid decreased in early to middle and increased towards end of lactation.
Availability: Items available for loan: UVAS Library [Call number: 1220,T] (1).
17.
Genetic Diversity And Differentiation Of Domestic Buffalo Of Pakistan Through Sky And Zfy Genes Of Y Chromosome
by Muhammad Mudassar Manzoor | Mr. Taneer Hussain | Mr. Muhammad Asif | Prof. Dr. Abu.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2011Dissertation note: Livestock sector plays a vital role in the economy of Pakistan. Main contribution of milk comes from buffaloes and cows. Water buffalo (Bubalusbubalis) is one of the major elements of livestock in the country and possess great importance for economy in the form of milk and meat productions. Nili, Ravi,Nili-Ravi, Kundi and Azakheli are major breeds of water buffalo to be found in different areas of Pakistan. Conventional classification of breeds was based on phenotypic traits. In some cases, recent genetic studies have found differences in the structure proposed. In buffalo ,one has to bear in mind that morphological changes were not the result of adaptation to the environment, but have a social significance thus may not be indicative of the genetic relationship. In recent years Y chromosomal genes have proved to be very useful for the determination of genetic relationship among population. Comparative studies have highlighted the advantages of the SRY and ZFY genes of Y chromosome.These genes have been considered as competent and powerful tool for the purpose of breed characterization and species identification of buffaloes. In livestock sector, water buffalo (Bubalusbubalis) has shown great prospective in numerous Asian countries including Pakistan. Unfortunately, there is lack of genetic data of different buffalo breeds like Nili-ravi and Kundi which needs to be established for their genetic identification.Blood samples from true representative animals of each of the two buffalo breeds (Nili-Ravi and Kundi ) were collected from different Government livestock farms and their respective home tractsin Punjab and Sindh respectively. DNA was extracted by inorganic method and amplification of the SRY and ZFY(exon 5) genes of Y chromosome was done with especially designed primers using Primer3 software in Molecular Biology and Genomics Laboratory at Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore.Specific primers are designed for these genes amplification. Then primers were optimized for successful amplification with minimum reagent concentration. PCR was performed for amplification of SRY and ZFY(exon 5)genes on each sample. Sequencing was conducted on amplicons to find out the different single nucleotide polymorphism (SNP) to make haplotypes with the help of bioinformatics software like Blast 2sequence and Neighbour Joining phylogenetic tree was constructed by using MEGA version 5 (Tamuraet al. 2011). The results obtained from this study now can contribute to the establishment of routine DNA typing service to the advantages of the buffalo in livestock industry.
Availability: Items available for loan: UVAS Library [Call number: 1378,T] (1).
18.
Molecular Characterization Of Local Isolation Of Staphylococcus Aureus On The Bsis Of 16S Rrna From Poulry And Their Transmission to Humans
by Muhammad Rizwan Ashraf | Mr. Muhammad Asif | Dr. Aby Saeed | Mr. Tanveer Hussain.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: Staphylococcus aureus is a widely distributed throughout the world and makes up the normal bacterial flora of skin and mucous membranes of man and animals. It is involved in suppurative wound infections in man and animals. Poultry industry has also been effected by S. aureus and causing great economic and health problems. The focus of the microbiology is to correctly identify S. aureus for the treatment of the animals. Molecular biology and biotechnology is proving a helping hand in the accurate identification of microorganisms through sequence analysis of 16S rRNA gene. The aim of this study was the molecular characterization of S. aureus from poultry and poultry farm workers through 16S rRNA analysis. Bacteria were collected from poultry and poultry farm human workers. All the samples were cultured and tested biochemically. In addition, peR amplification of 16S rRNA was performed in order to sequence the gene and further analyses through bioinforrnatics tools were performed. The aim of the study was the molecular characterization of S. aureus in poultry and humans through 16S rRNA sequencing, finding the phylogenetic relationships among S. aureus isolates and detection of zoonoses between poultry and human. 16S rRNA gene was amplified with peR primers and the sequence was compared with NeBI database reported S. aureus sequences. Resemblance was found between human and chicken isolates. Phylogenetic analyses were performed by using MEGA5 so ftware that also showed phylogenetic relationship among them.
Availability: Items available for loan: UVAS Library [Call number: 1393,T] (1).
19.
Production, Purification And Evaluation Of Anti Tetanus Serum
by Mian Muhammad Khubaib Sattar | Prof. Dr. Tahir Yaqub | Dr | Mr. Muhammad Zubair Shabbir.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2012Dissertation note: To produce anti-tetanus serum, ten female sheep of about 3 years of age are immunized with commercially available tetanus toxoid vaccine (Imatet™, Amson vaccines and Pharma) for eighteen weeks to these experimental animals with two weeks interval. Serum samples of all the sheep were also collected fortnightly and stored at -20 ºC.
ELISA was performed to determine the antibody (IgG) titer of all the test samples. Out of 90 samples, 20 samples showed tetanus antibody (IgG) titer of 100.8 I.U. or more, while 8 samples presented tetanus antibody (IgG) titer of 160.9 IU or more. Out of these 8 samples, 3 samples had tetanus antibody (IgG) titer of 190.9 I.U. or more. Maximum tetanus antibody (IgG) titer was 195.4 I.U. at day 120.
Three samples exhibiting maximum antibody titer (190.9 I.U., 195 I.U. and 195.4 I.U.) were mixed in equal quantities for purification of Immunoglobulins (IgG). A volume of 15 mL of aggregated serum samples was mixed with 15 ml of saturated ammonium sulfate having final concentration of 45 % in the mixture which is continuously stirred at room temperature for 1 hour. Mixture was centrifuged at 10000 rpm for 30 minutes in refrigerated centrifuge machine and dialyzed against 10 changes of PBS at 4 ºC. Desalting is checked with BaCl2 solution.
The purified tetanus immunoglobulins (IgG) were treated with papain to produce Fab Fragments. Then the protein content of the purified tetanus immunoglobulins and Fab fragments was estimated with Bradford protein Assay. BSA standard curve was used to produce a regression equation [Y (OD Value) = 0.218 + 0.033 X (Protein Concentration)] which was used for calculation of the protein contents of the samples.
The purified tetanus immunoglobulins and Fab fragments were tested for purity with SDS-PAGE analysis. Then in vivo toxin neutralization test was performed in mice to check the tetanus toxin neutralization ability of the sera produced.
Availability: Items available for loan: UVAS Library [Call number: 1420,T] (1).
20.
Production Of Azotobacter Vinelandii Mutant To Enhance The Production Of Alginate Through Submerged
by Sobia Saeed | Ms. Shagufta Saeed | Mr. Muhammad | Ms. Faiza Masood.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1523,T] (1).
21.
Designing Of Oligo Pool All For The Selection Of Superior Dairy Animals In Pakistan
by Kamran Abbas | Prof. Dr. Masroor Ellahi Babar | Dr. Aftab | Mr. Muhammad Asif.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Livestock has an important role in an agriculture based country like Pakistan with a large number of dairy animals. However the average daily milk yield of dairy animals is very low. There is need to improve milk yield by the selection of superior dairy animals using latest genomic selection procedures. Selection of superior animals on the basis of genetic markers has a tremendous potential for breed improvement across the globe. Substantial advances have been made over the past decades through the application of molecular genetics used in industry programs for several decades and is growing, the extent of use has not lived up to initial expectations. Most applications to date have been integrated in existing programs on temporary basis.
Among various molecular markers the Single Nucleotide Polymorphism (SNP) is one of the major genetic marker used worldwide. Through SNP genotyping selection of phenotypic superior animals can be done. There are many techniques used for SNP genotyping but the most advanced technique is Veracode GoldenGate Assay by Illumina. Illumina's VeraCode technology with the BeadXpress (BX) Reader is ideal for high-throughput small to mid-scale genotyping studies and SNP validation. BX leverages the power of digital holographic codes and the robust GoldenGate Genotyping Assay to provide a detection method for multiplex assays requiring high precision, accuracy, and speed. A custom assay of 48, 96, 144,192 and up to 384-SNPs OPA (Oligos Pool All) is designed using Illumina's Assay Design Tool and manufactured by Illumina.
As a first step for designing of Veracode GoldenGate Assay the development of Oligo Pool All (OPA) is necessary. The OPA was designed by using the genes for milk production, growth, fertility, health and other performance traits. The SNP's in these genes was searched from different gene banks and after proper arrangement the files were sent to Illumina for scoring. After scoring the OPA was finalized for the Veracode GoldenGate Assay for the selection of superior dairy animals in the country using the highly robust BeadXpress technology. The development of OPA for the selection of superior dairy animals was done for the very first of its kind based on modern technology, Veracode GoldenGate Assay in Pakistan. This will greatly help the livestock and dairy development departments, livestock owners, breeders, forensic agencies and researchers to use this unique panel of molecular markers for the selection of superior animals on the basis of marker assisted selection.
Availability: Items available for loan: UVAS Library [Call number: 1528,T] (1).
22.
Biochemical Identification Of Various Causes Of Anemia In Females From District Pakpattan
by Hafiz. Muhammad Toqeer | Dr. Muhammad Tayyab | Dr. Abu Saeed Hashmi | Mr. Muhammad.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Anemia is estimated to be affecting almost 600 millions people all over the globe and is regarded as deficiency in Hemoglobin concentration. The decreased amount of hemoglobin in blood could not been able to fulfill the oxygen demand of tissues in body. Keeping in view the above situation, a study was planned to investigate the various types of anemia in dist. Pakpattan.
One hundred blood samples were collected from females randomly selected from various parts of district Pakpattan. The samples were divided into two groups on the basis of age. Group A contains the patients with age between 14 to 26 years where as Group B consist of patients with age 27 to 40 years. Samples were processed in-order to estimate Complete Blood Count, serum iron level, serum ferritin levels, vitamin B12 assay and HPLC based estimation of various variants of hemoglobin.
The results demonstrated that 62% of the total female population of dist. Pakpattan was found to be anemic. Among Group A, 66.66% were anemic due to iron deficiency and 33.33% were due to chronic disease. Group B contained 59.09% anemic, out of these patients, 57.69% were anemic due to iron deficiency, 38.46% due to chronic disease and 2.27% due to deficiency of Vitamin B12. Iron deficiency was found to be the major cause of anemia that is followed by anemia due to chronic disease and Vitamin B12 deficiency. The intensity of anemia was 5% higher in young age females (Group A) as compared to the elder age females (Group B).
This work provided the information about the prevalence of various types of anemia in the population of dist. Pakpattan. The data will be helpful for developing strategy for the control of anemia in future. Further study with a large number of samples, is required throughout the country for the establishment of a data base that will be a good step to control various types of anemia.
Availability: Items available for loan: UVAS Library [Call number: 1611,T] (1).
23.
Qualitative Comparison Of Yoghurt Prepared From Conventional And Probiotic Culture
by Muhammad Ali | Prof. Dr. Muhammad Ayaz | Mr. Muhammad Junaid | Mr. Nisar.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1626,T] (1).
24.
To Investigate The Effects Of Heavy Metals Toxicity On Vital Organs Of Rohu (Labeo Rohita)
by Ghina Islam | Prof. Dr. Muhammad Ashraf | Mr. Muhammad Hafeez-ur-rehman.
Material type: ; Format:
print
Publisher: 2013Dissertation note: At the time of collection of samples Temperature, Dissolved Oxygen, Total Dissolved Solids (TDS), Conductivity and pH were determined (YSI Digital meters) of selected sites for any possible relationship between water quality parameters, heavy metal concentrations and their effects on fish body. Fish from the selected water bodies was blotted dry and then weighed individually. After wet digestion of the sampled fish, the volume was prepared for the determination of heavy metals viz. chromium, copper, cadmium, lead, nickel and manganese. Samples for histological studies were collected on monthly basis. Slides were prepared. The results of this study provided valuable information on the metal contents in fish from different sampling stations. Fish from Balloki Headworks exhibited the highest tendency of accumulation of cadmium, lead and chromium in liver and intestine while it was the minimum in fish collected from controlled fish ponds from Ravi Campus Pattoki. Accumulation of these metals was, however, the minimum in fish muscle collected both from Fish ponds Pattoki and Balloki Headworks. The present situation can be considerably improved by taking effective measures such as better cleaning of waste water from the industrial enterprises and urban agglomeration as well as by installing new waste water treatment plants.
Availability: Items available for loan: UVAS Library [Call number: 1660,T] (1).
25.
Evaluation Of Cytotoxicity And Antiviral Activity Of Moxidectin Against Influenza Virus H9
by Rabia Hameed | Prof. Dr. Muhammad Ashraf | Dr. Aftab anjum | Mr. Muhammad Adil Rasheed.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1665,T] (1).
26.
Evaluation Of Cytotoxicity And Antiviral Activity Of Ivermectin Against Newcastle Disease Virus
by Sidra Azeem | Prof. Dr. Muhammad Ashraf | Dr. Aftab | Mr. Muhammad Adil Rasheed.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1691,T] (1).
27.
Preservation And Developemental Study Of Bloody Fingerprints From Buried Substrates At The Crime Scene
by Shahid yousaf | Mr. Muhammad Akhtar Ali | Dr. Mohammad Ashraf Tahir | Dr. Muhammad.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1751,T] (1).
28.
Effect On Hematological Indices And Serum Mineral Profiles Of Beetal Goats In District Swat At Different Altitudes
by Faisal Anwar | Dr. Imtiaz Rabbani | Dr. Hafsa Zaneb | Mr. Muhammad Imran Khan | Faculty of Biosciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Livestock is the major source of income of rural population. Approximately 70% of the people in rural areas depend directly or indirectly on livestock for their house hold income and nutrition. Livestock efficiency mainly depends on their health and well-being. Blood is one of the reliable medium from which we can evaluate the health condition of an animal. Livestock in Pakistan is reared in various altitudes. Information on various physiological indices in goats related to altitude is scarce for Pakistan. The present has been conducted at three different altitudes, two altitude groups from district Swat and one group at Peshawar Khyber Pakhtunkhwa.
A total of 60 blood samples were collected from three different altitudinal groups and then the hematological parameters was determined through hematological analyzer and serum concentration of Calcium and Potassium by flame photometer, Phosphorus by UV-VIS spectrophotometer, Chlorine by silver Nitrate titration method and Iron by Drabkin method.
The White blood cell count (WBC) found 10.47±0.34 × 103/µL at altitude 1177 feet, 8.33±0.13 × 103/µL at altitude 2863 feet and 11.52±0.52 × 103/µL at altitude 4200 feet from sea level. The lymphocytes count (LYMP) found 28.90±0.46 percent at altitude 1177 feet, 28.55±0.60 percent at altitude 2863 feet and 31.15±0.99 percent at altitude 4200 feet from sea level. The granulocytes (GRAN) count found 65.10±0.85 percent at altitude 1177 feet, 65.89±1.41 percent at altitude 2863 feet and 67.23±1.87 percent at altitude 4200 feet from sea level. The red blood cell count (RBC) found 10.98±0.22 million/µL at altitude 1177 feet, 11.65±0.15 million/µL at altitude 2863 feet and 14.62±0.26 million/µL at altitude 4200 feet from sea level. The hemoglobin count (Hb) found 5.17±0.19 g/dL at altitude 1177 feet, 7.23±0.10 g/dL at altitude 2863 feet and 10.96±0.59 g/dL at altitude 4200 feet from sea level. The Hematocrit count (HCT) found 29.14±0.39 percent at altitude 1177 feet, 28.94±0.42 percent at altitude 2863 feet and 30.69±0.48 percent at altitude 4200 feet from sea level. The mean corpuscular volume (MCV) found 28.83±0.43femtoliter at altitude 1177 feet, 31.06±0.37 femtoliter at altitude 2863 feet and 35.94±0.39 femtoliter at altitude 4200 feet from sea level. The mean corpuscular hemoglobin (MCH) found 5.27±0.12 picogram at altitude 1177 feet, 5.73±0.10 picogram at altitude 2863 feet and 6.78±0.09 picogram at altitude 4200 feet from sea level. The mean corpuscular hemoglobin concentration (MCHC) found 29.82±0.49 g/dL at altitude 1177 feet, 32.10±0.57 g/dL at altitude 2863 feet and 34.32±0.39 g/dL at altitude 4200 feet from sea level. The platelets (PLT) count found 270.9±3.81 103/µL at altitude 1177 feet, 294.95±3.61 103/µL at altitude 2863 feet and 283.50±5.28 103/µL at altitude 4200 feet from sea level.
The Calcium (Ca) level found in serum was 8.79±0.23 mg/dL at altitude 1177 feet, 9.44±0.16 mg/dL at altitude 2863 feet and 9.80±0.16 mg/dL at altitude 4200 from sea level. The Chloride (Cl) level found in serum was 102.91±0.39 m?q/dL at altitude 1177 feet, 104.08±0.42 m?q/dL at altitude 2863 and 105.50±0.57 m?q/dL at altitude 4200 feet from sea level. The Phosphorus (P) level found in serum was 3.98±0.02 mg/dL at altitude 1177 feet, 5.52±0.08 mg/dL at altitude 2863 feet and 6.34±0.08 mg/dL at altitude 4200 feet from sea level. The Potassium (K) level found in serum was 3.98±0.04 m?q/dL at altitude 1177 feet, 4.61±0.06 m?q/dL at altitude 2863 feet and 4.93±0.04 m?q/dL at altitude 4200 feet from sea level. The Iron (Fe) level was found 87.12±0.97 µg/dL at altitude 1177 feet, 97.44±0.67 µg /dL at altitude 2863 feet and 106.35±0.87 µg/dL at altitude 4200 feet from sea level.
A significant difference was found between different altitudes in WBC count, LYMP percent, RBC count, Hg level, HCT percent, MCV, MCH, MCHC, PLT, Ca level, Cl level, P level, K level, Fe level and no significant difference was observed in GRAN percent. This study generated a better understanding that hematology and serum mineral profile has been affected as an increase occurs in the altitudes and it reflects that increase in RBCs, Hematocrit and hemoglobin level at different altitudes also the serum mineral level goes high as there is an increase in the altitudes shows the adaptation of animal to the environmental condition and this study will be helpful in disease investigation and management of animals at different altitudes and different stress conditions. Further studies are been required to evaluate the effect of altitude, feed intake, nutrition, environmental stress and climatic condition on the hematology and serum mineral profile of different breeds of small ruminants and large ruminants.
Availability: Items available for loan: UVAS Library [Call number: 1777,T] (1).
29.
Comparative Anthelmintic Efficacy Of Calotropis Procera Leaves And Moxidectin With Particular Reference To Biochemical Changes in Sheep
by Qamar Niaz | Prof. Dr. Muhammad Ashraf | Dr. Aneela | Mr. Muhammad Adil Rasheed.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Background:
This project was designed to evaluate the anthelmintic efficacy of Chloroformic extract of Calotropis procera leaves against sheep nematodes. For this purpose hundred sheep positive for nematodal infection in BLPRI Kherimurat were selected after fecal examination.
Methodology:
The hundred animals were divided into 5 groups (Group A, Group B, Group C, Group D and Group E) having 20 animals in each group. Fecal eggs counts (EPG) and biochemical tests like the Alanine aminotransferase (ALT), Aspapartate aminotransferase (AST), Alkaline phosphatase (ALP), Albumin, Bilirubin, Creatinine and Urea were done on the day zero of the treatment. For further study, Group A served as an un-treated control. Group B, Group C and Group D were given the Chloroformic extract of Calotropis procera leaves at the dose rate of 3, 6 and 12mg/kg body weight by oral route respectively. Group E was given the Moxidectin at the dose rate of 0.2mg/kg body weight by subcut route. The biochemical tests on the day 3 showed no change in the normal level of the ALT, AST, ALP, Albumin, Bilirubin, Creatinine and Urea. The fecal EPG of all the groups were done on the day 7th and day 14th post treatment. The efficacy of the Group A and Group B was 0% both on day 7th and day 14th post treatment. The efficacy for the Group C was 19.40% and 42.53 % on the day 7th and day 14th post treatment respectively. The Group D showed the efficacy of 80.215% on day 7th and 91.36% on the day 14th of the treatment. The efficacy for the Group E was 85.97% and 93.29% on the day 7th and 14th respectively.
Statistics:
The data from the fecal eggs count and biochemical tests were analyzed by using the ANOVA and LSD.
Conclusion:
The Chloroformic extract of the Calotropis procera leaves have maximum anthelmintic effect at the dose rate of 12mg/kg body weight having no biochemical changes in the blood.
Availability: Items available for loan: UVAS Library [Call number: 1804,T] (1).
30.
Effect Of Different Physiological Stages On Heamatological And Biochemiscal Parameters Of Nili-Ravi Buffalo And Sahiwal Cattle
by M. Zohaib Aslam | Dr. Imtiaz Rabbani | Dr. Muhammad Shahbaz Yousaf | Mr. Muhammad.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: 6.1 Background
The proper disease diagnosis in large ruminants is required for overcoming the economy loss due to livestock poor management. Blood biochemical and hematological parameters are one of an important medium to assess the physiological and pathological condition of animals. Any physiological and pathological change can be evaluated only if the normal values are available for comparison. Factors such as age, breed, geographical location, diet, sex, diurnal variation, physical activity, pregnancy and environment are known to influence the serum biochemistry and haematology of large ruminants. Our hypothesis was to evaluate the effect of different physiological stages (pre-pubertal and pubertal) on hematological and serum biochemical parameters of Nili-Ravi buffalo and Sahiwal cattle.
6.2 Materials and Methods
Sixty blood samples (30 Cattle and 30 Buffalo) were collected from Ravi Campus Pattoki, UVAS and divided on the basis of different physiological stages, pre-pubertal (10-12) and pubertal (22-24 months) into two groups. The collected samples used to estimate complete blood counts by hematological analyzer. The biochemical parameters include cholesterol, triglycerides, total protein, urea, AST and ALT by commercially available kits (Biogene and crescent).
6.3 Statistical Analysis
Statistical Package for Social Science (SPSS) version 17 used. Further T Test was used to compare the hematological and blood biochemical between two age groups. Differences was considered significant at P<0.05 (Steel et al.1997).
6.4 Results
The hematological parameters RBCs, Hb and PCV were showed a significant difference at Pre-pubertal and pubertal stage of both buffalo and cattle. Whereas the WBCs count, lymphocytes, granulocytes, monocytes, MCV and MCH were not found significantly different at pre-pubertal and pubertal stages of buffalo and cattle. The serum level of glucose recorded highest and urea level lowest at pubertal stage in both buffalo and cattle. The cholesterol and triglyceride were found significantly (p<0.05) lowest, while liver enzyme AST and ALT were found significantly highest with the aging of animal in both species. No significant changes were observed in total protein level at pre-pubertal and pubertal stage in buffalo and cattle.
6.5 Conclusions
The WBCs, RBCs, Hb, PCV were found significantly different between pre-pubertal and pubertal stages in both species. The values of theses parameters recorded highest at pubertal stage, while granulocytes, lymphocytes, monocytes, MCV and MCH were found non-significantly different. Different physiological stages had a significant effect on glucose, cholesterol, triglycerides, Urea, ALT and AST in both species. The results of this study indicated that different physiological stages must be taken as very important factor in determining the blood parameters.
Availability: Items available for loan: UVAS Library [Call number: 1806,T] (1).
31.
Isolation And Molecular Identification Of Enterotoxigenic Escherichia Coli Strains Isolated From Raw And Pasteurized Milk
by Rahman Ullah | Dr. Imran Javed | Mr. Muhammad Junaid | Prof. Dr.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1816,T] (1).
32.
Evaluation Of Probiotic Potential Of Locally Characterized Lactobacillus Spp. In Broiler
by Saima asghar | Dr.Muhammad Nawaz | Mr. Muhammad Asad ali | Prof. Dr.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1931,T] (1).
33.
Role Of Aqueous Extracts From Zingiber Officinalis (Ginger) And Allium Sativum (Garlic) On Pathogenicity Of Avian Influenza Virus H9N2 on Chicken Embryoes
by Amir Rasool | Dr. Muti ur Rehman Khan | Mr. Muhammad | Mr. Muhammad Asad Ali.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2127,T] (1).
34.
Detection Of Influenza A Virus Contamination In Newcastle Disease Live Virus Vaccines And Their Pathological Effects On Visceral Organs
by Munir Hussain (2004-VA-64) | Mr. Muhammad Saeed Imran | Prof. Dr. Asim Aslam | Dr. Shafqat Fatima Rehmani.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Poultry is one of the most vibrant commercial sector which is playing a vital role to
bridge the gap between supply and demand of animal protein foods to cater for its ever
increasing human population 2.1 per cent annually in Pakistan (Sahota et al. 2003).
Vaccination is one of the most effective way to prevent the poultry birds from the
specific diseases. Disease producing microorganisms can be classified smallest to largest as
viruses, bacteria, fungi, protozoa and parasites. All, except the viruses are sensitive to drugs
when outbreaks occur. Vaccination is basically the introduction of a specific biological substance
(antigen) into the bird to stimulate the antibodies formation or immunity to a particular disease.
Usually the biological substance is avirulent the live disease organisms, which are capable to
protect the bird against the particular disease by producing an immune response. Presence of
these organisms (antigen) in the blood stimulates the body's defense mechanism to produce
antibodies that neutralize the disease causing organisms when the bird is exposed to them
(Kamboh et al. 2009).
A danger of such type of live vaccines is that the live microbes can back mutate to a
virulent form. While, dead vaccines that contain whole killed (usually by formalin or phenol)
microbes are safe. They may contain little or no extraneous material and therefore tend to
produce fewer adverse effects (Palombo and Semple 2001). The vaccines that contain dead
organisms are safe with respect to residual virulence and are easy to store, since organisms are
already dead. While live vaccines may possess residual virulence for the animal by reversion of
avirulent organisms to fully virulent type or spread to nonvaccinated animals. Dead vaccines
have very little risk of ‘alive’ contamination, while live vaccines always run the risk of
contamination with unwanted organisms; for instance, outbreaks of reticuloendotheliosis in
Introduction
______________________________________________________________________________
2
chickens in Japan and Australia have been traced to contaminated Marek’s disease vaccine
(Tizard 1995).
Avian Influenza viruses typically produce Syndromes ranging from asymptomatic
infection to respiratory disease and drops in egg production to severe, systemic disease with near
100% mortality (Olsen et al. 2002). Avian influenza initially was recognized as a highly lethal,
systemic disease (i.e., highly pathogenic). HPAI was known by various name including fowl
plague, fowl pest etc. Avian Influenza viruses are classified in the family orthomyxoviridae,
genus influenza virus A (Garten et al. 2009). Avian influenza viruses can be categorized into
four clinical groups:1) highly virulent, 2) moderately virulent, 3) mildly virulent, and 4)
Avirulent (Swayne and Suarez 2000). Avian Influenza further sub type based on serologic
reaction of HA and NA surface glycoproteins. Fifteen sub types of HA and nine sub types of
NA are recognized (Swayne and Suarez 2000). MP AI viruses in domestic poultry produce
clinical sign reflect abnormalities in the respiratory, digestive, urinary and reproductive organs
(Allwright et al. 1993). To date, naturally occurring highly virulent influenza A viruses that
produce acute clinical disease in chickens, turkeys and other birds of economic importance have
been associated only with the H5, H7 and H9 subtypes. Influenza A viruses of subtype H9 are
now considered to be wide spread in poultry and have demonstrated the ability to infect humans
(Fedorko et al. 2006).
To date, all outbreaks of the highly pathogenic form have been caused by influenza A viruses
of the subtypes H5 and H7. The disease is transmitted horizontally by direct contact through
contamination. There is little or no evidence of vertical transmission (egg-borne infection).
However, eggshell surfaces can be contaminated with the virus (Potima 2007). Wild and
domesticated water fowl is the major natural reservoir of influenza A viruses. Representatives of
Introduction
______________________________________________________________________________
3
all of the different subtypes of avian influenza A virus have been isolated from birds, particularly
from aquatic species such as ducks, geese, and gulls (Karasin et al. 2000). Wild birds such as
geese, ducks and game birds; they can be carriers of even highly pathogenic strain H5N1
shedding the virus in their feces without clinical signs of disease.
Thus, the present study was carried out to examine the viral contamination (Influenza A
virus) in poultry vaccines manufactured locally and imported from different countries of the
world in Pakistan. The findings of the study have helped us to see the Avian Influenza A virus
contamination in vaccines which are used in field conditions and also help to evaluate the purity
of vaccines. The RT-PCR based technology has been described for the detection of different
RNA viruses such as Newcastle disease virus etc. (Payne et al. 1981) revealed contamination of
vaccines with ALVs, specifically in two Marek´s vaccines, which confirms that these agents are
potential contaminants of viral vaccines applied in poultry. This assay has meant a considerable
advance due to a higher sensitivity and specificity upon differentiating the subgroups compared
with ELISA. It is quicker test for detection of RNA viruses than the viral isolation, which
requires until 10 days and it needs detection by ELISA for the identification result. Availability: Items available for loan: UVAS Library [Call number: 2212,T] (1).
35.
Effect of Timing of Artificial Insemination After Controlled Internal Drug Release (CIDR) Device Removal And Or CIDR + GnRH Based Estrus Synchronization Protocols In Nili Ravi Buffalo
by Muhammad Shafiq Haider (2008-VA-226) | Prof. Dr. Naseem Ahmed | Mr. Muhammad Usman Mehmood | Prof.Dr. Muhammad Sarwar Khan.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Blank CD Availability: Items available for loan: UVAS Library [Call number: 2240-T] (1).
36.
Development And Evaluation Of Mozzarella Cheese Influenced By Type Of Starter Culture And Fat Content Of Milk
by Imran Taj Khan (2013-VA-866) | Mr. Muhammad Junaid | Dr. Muhammad Ayaz | Mr. Muhammad Saadullah.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Pakistan is the 4th largest milk producing country in the world having 50.99 million tons/year in which buffalo and cow add 61% and 35% share, respectively to total milk production in Pakistan, while the rest 5% of the total milk is contributed by other species like small ruminants and camel. Out of the total produced milk only 7-8% of the milk is processed and passes via milk supply chain while the remaining is distributed as raw milk (GOP, 2013-14). Processing of milk into cheese is a small segment in Pakistan, moreover the eating habits are not developed in Pakistani population along with the increase in price of local as well as imported cheese.
The manufacturing of cheese is one of the best examples of preserving milk, dating back from 6000-7000 BC till now. Cheese like other value added dairy products are among the highly nutritious diet of the world. Cheese comes in great variety of tastes and flavors, in a wide array of shapes, and are highly healthful and nutrimental (Walstra et al. 2006). The utilization of cheese in the form of pizza garnishes, cheese mixes, salads, sandwiches, stuffing has expanded because of dietary resemblance, ease and extensibility being used and cheese quality (Fox et al. 2000). Cheese manufacturing is increasing worldwide at the rate of 4.9% every year (Valhovic et al. 2014).
Cheese is the value added dairy product acquired by the seepage (of fluid) after coagulation of milk proteins through utilization of rennet and starter. Cheese is a mainstream nourishment because of its differing qualities in application, wholesome quality, accommodation and appealing taste. The assorted qualities are because of an expanding information of the innovation of cheese making and the natural chemistry and microbiology of cheese maturing (Sulieman et al. 2013). There are 500–800 assortments of cheese accessible in the universal business sector (Razzaq, 2003).
Mozzarella cheese is placed in the group of “Pasta filata” or stretched cheeses. Stretching is a treatment that gives the curd elasticity which is a dominating functional characteristic of Mozzarella cheese. Melting and stretching are the most important characteristics of Mozzarella cheese which is highly acknowledged in the making of Pizza as it is a chief ingredient (Owni et al. 2009).
Mozzarella cheese is a standout amongst the most prominent cheese varieties on the planet, in light of its essential use in the pizza topping (Kindstedt et al., 2004). The funtional attributes of Mozzarella cheese like shape, composition, meltability, stretchbility and color are changed by the components like milk composition, culture source or type and maturing environment dominating in the midst of the cheese availability (Luecy et al. 2003).
Mozzarella cheese is produced utilizing a paired lactic acid bacteria starter cultures of Streptococcus salvarius ssp. thermophilus and Lactobacillus delbrueckii ssp.bulgaricus (Ahmed et al. 2011).
At a certain point, Mozzarella was made just from water buffalo milk. Presently, it is normally produced using milk of bovine, goat and sheep. There are two types of Mozzarella cheese; i.e. regular Mozzarella which is accessible in low-fat and nonfat structures and has a semi-delicate in texture, versatile composition and is drier than fresh Mozzarella while, Fresh Mozzarella is produced using entire drain and has a gentler texture and sweet pleasing flavor and is commonly pressed in water or whey (Ibrahim, 2003).
Mozzarella cheese also has numerous therapeutical advantages; it is a decent wellspring of protein, vitamins and minerals. Utilization of Mozzarella cheese may secure against gout, an agonizing condition that outcomes in the development of uric acid stones in the joints. The calcium found in Mozzarella cheese additionally has its commitment in body weight reduction and gives insurance against breast tumor and metabolic disorder, which is a gathering of conditions that build the danger of creating cardiovascular disease or stroke (Ibrahim, 2003).
Low fat Mozzarella cheese is a nice wellspring of protein and calcium, furthermore it is non calorie-dense and less in saturated fat, which can prevent from cardiovascular illness, type 2 diabetes, joint inflammation, and memory loss, also block weight reduction endeavors upon consumption. Eating low fat ("part-skim") or fat-free Mozzarella cheese is an extraordinary approach to gain by its alimental advantages while minimizing unfortunate additional items. The calcium in Mozzarella cheese aides keep up healthy pulse rate and blood pressure (Bauer, 2014).
Mozzarella cheese production in the country in demand based being its most use in the pizza sector while its limited use as fresh product. Moreover the industry is using commercial imported culture for production of this cheese. The high price of the culture is one of the factor in the high price of the final product. Therefore the present study was designed with an objective to utilize the local culture for production of Mozzarella cheese. The idea was to maintain the quality of product at its higher regime. Furthermore its quality attributes were compared with the standard cheese produced from commercial culture. In addition the effect of change in fat level of milk on the quality and overall acceptability of the produced Mozzarella cheese was assessed.
Availability: Items available for loan: UVAS Library [Call number: 2266-T] (1).
37.
Identification Of Variations In The Coding Region Of Myostatin Gene In Thalli And Pakkarakul Sheep Breeds Of The Punjab
by Armughan Ahmed Wadood (2006-VA-38) | Dr. Afzal Ali | Prof. Dr. Khalid Javed | Mr. Muhammad Saadullah.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: In a developing country like Pakistan, livestock sector has a significant importance.
Meat is an important food component in human diet and its source varies from different
organisms like cattle, buffalo, camel, deer, sheep, goat, rabbit and chicken etc. Small
ruminants especially sheep, has a unique role in meat production, due to its feeding and
adaptive behavior. The myostatin (MSTN) gene is known as a candidate gene for
development and growth of livestock because it plays its major role in muscle growth.
Selection is an important tool to improve the meat production and thus enhance the economic
conditions of livestock farmers. For this purpose genetically characterization of meat type
animals, especially the indigenous sheep breeds is not common. The variations in the
myostatin gene have been extensively studied and reviewed in small and large ruminants in
the world but it has been scanty studied in sheep breeds of Pakistan. Thalli and Pak-Karakul
are well known sheep breeds present in Punjab, Pakistan. This study aims to characterize the
genetic variation in the myostatin gene in Thalli and Pak-Karakul sheep breeds.
In this research study, twenty adult animals of two different sheep breeds (Thalli and
Pak-Karakul) were selected from Small Ruminants Research and development Centre, Rakh
Khairewala, District Layyah, Pakistan. Five mL blood sample was collected from each
animal in a 15mL falcon tube containing anticoagulant. Primers were designed by using
Primerfox online software. Primers were optimized using specific protocol and PCR was
performed. DNA was extracted using modified inorganic method of Sambrook et al. (1989).
PCR was carried out using all primers and later sent for sequencing to 1stBase Laboratories,
Singapore. Molecular analysis was done using CodonCode Aligner and MEGA6 softwares.
Ten samples of each breed were sequenced to detect polymorphism in both Thalli and
Pak-Karakul sheep populations. Sequencing revealed G↓T transition at 3995 position in
Summary
44
genome (accession number, DQ530260.1) in Thalli and similar transition in Pak-Karakul at
the same position. In Thalli sheep 50 % of experimental animals were heterozygous, higher
level of heterozygosity makes it a potential candidate for higher growth rates. On the other
hand the Pak-Karakul sheep is medium weight breed and frequency of mutant allele was 0.2
or 20%. The observed heterozygous individuals were also with higher live body weights. The
gene frequency shows that this breed has medium potential for its body growth. The
information‟s so generated and further association studies in both breeds will be helpful in
devising breeding plans for increasing mutton production at national level. Availability: Items available for loan: UVAS Library [Call number: 2288-T] (1).
38.
Evaluation of Growth Performance, Carcass Characteristics And Economic Appraisal of 3 Broiler Strains Under 4 Brooding Sources And Varying Feeding Regimens In Termianal Phase
by Muhammad Shabir Shaheen (2013-VA-778) | Dr. Shahid Mehmood | Prof. Dr. Athar Mahmud | Mr. Muhammad Imran.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Theses submitted with corrupt cd. Availability: Items available for loan: UVAS Library [Call number: 2351-T] (1).
39.
Assessment Of Correlation Between Food Choices And Fertility Status In Females
by Tamkenat Mansoor (2013-VA-923) | Dr. Muhammad Nasir | Ms. Amina Chughtai | Mr. Muhammad Bilal.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note:
The changing lifestyles and eating patterns have resulted in a number of fertility related problems, especially in females. Initially it was thought that only morbidly increased or decreased body weights were the causative factors in cases of infertility and sub fertility but it has been observed that a lot of women with normal body weight are also increasingly finding it difficult to conceive. Similarly obese and underweight women have also been seen to reproduce normally. Thus, in order to have a better understanding of the relationship between food and fertility we need to compare the eating patterns of fertile women with those of infertile ones. Various eating patterns are associated, either negatively or positively, with the fertility status of females.
At least 50 fertile and 50 infertile females, attending the OPDs at health centers in Gujrat, during the time of 6 months or until the benchmark of 50 control and 50 cases is not achieved, will be recruited and interviewed through a proposed questionnaire. Other than Food frequency checklist, social, medical and sexual history of the females will also be included in the questionnaire.
Filled questionnaires will be analyzed using Minitab. Comparisons will be made between the food choices of fertile and infertile group using t-test and other relevant tests.
The results of the proposed study will help to assess the association between the eating patterns and fertility status of females and help the practitioners to educate and better assist their patients to conceive successfully.
Availability: Items available for loan: UVAS Library [Call number: 2415-T] (1).
40.
Assessment Of Nutritional Status Of End Stage Renal Disease Patients On Maintained Hemodialysis
by Ayesha Batool (2014-VA-548) | Ms. Amina Chughtai | Dr. Sana Ullah Iqbal | Mr. Muhammad Bilal.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: End stage renal disease patients on MHD (maintained hemodialysis) have critical nutritional status. The state of nutrition is always critical for the recovery from any disease. An optimal health status assures good quality of life. The study aimed to assess the nutritional status of patients diagnosed with ESRD on maintained hemodialysis. Caloric and protein intake of patients was calculated and compared with standards. Inadequate dietary intake leads to protein energy malnutrition in patients with end stage renal disease on maintained hemodialysis. A sample of 240 patients identified with end stage renal disease (age 40-60 years) visiting outdoor patient department, department of nephrology, Sheikh Zayed Hospital and nephrology department Mayo Hospital, Lahore was selected. Sample was calculated using Yummny formula. Sample was selected through purposive sampling. Direct Nutrition assessment will be conducted through ABD. Anthropometric measurements included BMI, MAMC and TAPM (Appendices I). Biochemical tests included Creatinine, BUN, Serum albumin, total proteins and hemoglobin. Dietary intake was assessed through 24 hour diet recall and FFQ. PG-SGA form was used for screening of patients for malnutrition. The study found that the nutritional status of ESRD patients on MHD is critically poor. 82% of the subjects of the study were moderately to severely malnourished. BMI of most of the patients were normal and thus it is not a reliable parameter to assess malnutrition among critically ill patients. The dietary intake data had shown significant difference between intake and requirements of calories (kcal/day) and proteins (gm/day). The MAMC and TAPM results interpreted that lean muscle mass is depleted in more than 70% of the patients. TAPM values were significantly related with protein intake and PS-SGA score. Thus, it is a reliable parameter for the assessment of malnutrition. Descriptive
Summary
74
statistics, regression analysis, t-test, correlation, chi square and cross classification table was used to analyze data. Data was presented in form of graphs and tables. Statistical software SPSS version 22 was used to analyze data. Data thus obtained will serve as baseline data for improvement of nutritional status of ESRD patients. The study also validated the nutritional markers and parameters, which will be helpful in assessment of critically ill patients. Availability: Items available for loan: UVAS Library [Call number: 2605-T] (1).
41.
Isolation, Molecular Identification And Antibiotic Resistance Pattern Of Salmonella Enterica From Fancy Birds
by Aqeela Kousar (2010-VA-303) | Mr. Muhammad Asad Ali | Prof. Dr. Aftab Ahmed Anjum | Prof. Dr. Mansur-ud-Din Ahmad.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Salmonellosis is a disease with serious health issues related to food borne illness and
most of world’s population is suffering from it. Early diagnosis in case is very important for
treatment of disease. Salmonellosis may hidden as a carrier state, acts as zoonotic components
for transmission of disease. Therefore the test with more diagnostic value needs to be developed
like Polymerase chain reaction after culturing and microbiological examination.Salmonella
enterica infections continue to pose a significant risk for poultry industry and fancy birds.
Salmonella infections have been controlled by antibiotics but in recent times antibiotic resistance
in microorganisms especially in Salmonella is a global health issue. Antibiotic resistant
Salmonella has further compounded the problem. Poultry isolate of Salmonella enterica (n=150)
were procured from Jallo park, Safari park and household pets which are taken to Pet Centre
University of Veterinary and Animal Sciences Lahore then brought to Department of
microbiology UVAS Lahore and identified by biochemical testing, morphology, staining
characters and genus specific PCR. Antibiotic Susceptibility was checked by disc diffusion
method against amoxicillin (30μg), ampicillin (10μg), cefixime (5μg), , ceftazidime (30μg),
ceftriaxone (30μg), ciprofloxacin (5μg), gentamicin (10μg), nalidixic acid and tetracycline
(30μg) and resistant pattern was 100 % in ampicillin and tetracycline and 41.18% and 58.82% %
in gentamicin and ciprofloxacin respectively while antibiotic show 0% resistance. Fancy birds
are carriers of drug resistant Salmonellae.
A total of 150 samples collected from Zoo Lahore, safari park and household pet fancy
birds each of n=50. Samples will enriched by non-selective and selective media, After isolation
on selective media macroscopic, biochemical analysis and microscopic examination done. DNA
Summary
53
extracted from culture isolated from cloacal swabs and polymerase chain reaction performed
using primers. Amplication will be observed using Agarose gel electrophoresis.
Research highlighted the prevalence of Salmonella in fancy birds and its possibility of
transmission to human beings. Research also provided data on antibiotic resistance in
Salmonellae from fancy birds and its possible role in ever increasing problem of antibiotic
resistance. Availability: Items available for loan: UVAS Library [Call number: 2615-T] (1).
42.
Effect Of Various Classroom Educational Activities On Fresh Fruit Intake Of Children
by Anum Zafar (2014-VA-533) | Ms. Tahreem Hussain | Ms. Amina Chughtai | Mr. Muhammad Bilal.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Children of age group 3-4 years are developing their dietary habits. Multiple factors affect their eating preferences. Intake of fresh fruits is observed to be less in this age group. Initiative to improve fresh fruit intake during this age is of utmost importance as fruits are rich in micronutrients. The proposed study attempts to find out the effect of class activities on fresh fruit intake of children.
The current study hypothesized that class learning activities can improve the consumption of fresh fruits in children and the results of the current study indicates that fresh fruit intake can be increased by different educational activities.
A sample of 100 children 3-4 years of age enrolled in The Educators (Shahdara campus) participated in the study. They were divided in four equal sub groups, 25 children in each group. Each group was given the same weighed (109grams) fruit bowls for 20 mins. One group was the control group. In contrast the other three experimental groups received the treatment in the form of different class activities like animated movie, storytelling and the talk session. The fruit bowls were served after these activities and then the weight of the fruit bowls were recorded again. The experiment was performed thrice with a gap of one week to observe the difference. Those participants who were suffering from any type of allergy were excluded from this study.
Data of the leftover weight was analyzed statistically by SPSS version 21. Descriptive statistics (frequency/percentage), graphs, tables and graphs were used to analyze the data of different days of the treatment. One way ANOVA was used to check the difference between the outputs of different groups.
The result of the current study showed that participant with the treatment of storytelling ate more than any other treatment with lowest mean value of left over weight 14.36, however the group which receive the treatment of discussion had the mean value of 21.49 and the treatment with movie had the mean value of 34.02. Participant belong to the control group did not eat much fruit because they had highest mean value of leftover weight of 39.69.
Therefore it can be concluded from the current study that there is a positive effect of different class based learning activities on fresh fruits intake in children of age group 3-4 year.
Availability: Items available for loan: UVAS Library [Call number: 2659-T] (1).
43.
Effect of aqueous date palm (Phoenix dactylifera L.) fruit extract on antioxidant characteristics of ice cream
by Nadeem Abbas (2008-VA-412) | Dr. Muhammad Nadeem | Mr. Muhammad Junaid | Dr.Shahid Mehmood.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2017Dissertation note: The application of antioxidants extracted by the organic solvents have limited applications in foods due to their toxicity. Most of the antioxidants of date palm fruit (Phoenix dactylifera L.) are soluble in water. Antioxidant activity of water soluble natural antioxidants of date palm fruit using ice cream as an oxidation substrate has not been studied previously. This study has been planned with the objective to study the antioxidant characteristics and oxidative stability of ice supplemented with aqueous date palm fruit extract.
Date palm fruit extract were prepared by extracting 20 g date with 100 ml distilled water, followed by concentration and filtration. Ice cream were supplemented with date palm fruit extract at 200, 400, 600, 800 ppm and tertiary butylated hydroxy quinone 200 ppm concentrations. Ice cream were filled in plastic cups and stored at -18oC for 60 days.
Antioxidant characterization of date palm fruit extract was performed by yotal phenolic contents, DPPH free radical scavenging activity. Oxidative stability of ice cream was determined by peroxide value, anisidine value, changes in fatty acid profile sensory evaluation were performed at 0, 30 and 60 days of storage period.
Date palm fruit extract was characterized for antioxidant capacity, total phenolic contents of date palm fruit extract were 7.51% (GAE).DPPH free radical scavenging activity of date palm fruit extract was 78.8% as compared to 80.5%. Supplementation of ice cream with date palm fruit extract did not have any significant impact on fat, protein and total solids content.
Results indicated that peroxide value increased during the storage of 60 days in all the treatments and control. Rise in peroxide value was strongly correlated with the addition and dose of date palm fruit extract. Control had the highest peroxide value, followed by T1, T2, T3 and T4. Peroxide value of control and ice cream samples added with TBHQ (200 ppm) were not different
from each other (p<0.05). After 60 days of storage, anisidine value of control, T1, T2, T3, T4 and TBHQ added samples were 13.62, 10.58, 8.49, 7.36, 6.89 and 6.39, respectively.Flavor score of control, T1, T2, T3, T4 and TBHQ added ice cream were 8.2, 8.1, 8.0, 8.0, 8.1 and 8.2.
Date palm fruit extract 600 and 800 ppm can be sued for the long term stabilization of ice cream.
Availability: Items available for loan: UVAS Library [Call number: 2862-T] (1).
44.
Evaluating The Efects Of Prebiotics In Rehabilitation Of Gut Microbiota After Antibiotic (Cephalexin) In Rats
by Rabia Omer (2011-VA-512) | Dr. SanaullahIqbal | Mr. Muhammad Amir Iqbal | Dr. Sajid khan Tahir.
Material type: Publisher: 2017Dissertation note: Antibiotics are widely used to kill pathogenic bacteria that have adverse effects on human health. It has been seen that these kind of drugs causes imbalance of microbes because these are not only target the pathogens but also suppressing the growth of beneficial bacteria. Beneficial bacteria such as bifidobacteria and lactobacilli species conquer many benefits for human health. That’s why there is a need to rehabilitate these valuable microorganisms. The worthwhile approach in this regard is the use of galacto-oligosaccharides(GOS).
Adult male Wistar rats (n= 24) were used in present study. Rats were divided into 4 groups having 6 rats in each group. Group G1 was a control normal that was fed on a basal diet. Group G2 was treated with an antibiotic cephalexin for five days. Moreover, Group G3 wasgiven GOS as well as antibiotic. In Group G4, firstly antibiotic was given for specific duration and after that GOS was given for the rest period of time. The fecal sample were collected from rats with time interval of 0, 5 , 10 and 15day of antibiotic therapy or treatment and were analyzed for bacterial population especially Bifidobacteria, Lactobacilli, E. coli and total plate count using selective media.
The results of bifidobacteria and lactobacilli of group 3 were significantly same with group 1 at 15th day and were notably higher from group 1 at 20th day. In addition the beneficial species in group 2 were decreased at 5th day that significantly different with group 1 and didn’t recover till at 20th day. The colonies of E. coli in various groupswere different at various time intervals. Moreover the results of TPC were based on the treatments. It was significantly higher in group 3 that was given antibiotic with prebiotics while it was significantly lower in group 2 as compared to normal.The data were analyzed using One way Analysis of variance (ANOVA). The group differences were compared by the Duncan’s Multiple Range Test. Differences were considered significant at P < 0.05.
This study helped to turn down the side effects of antibiotic as well as maintain the normal micro-flora by rehabilitate the beneficial species such as bifidobacteria and lactobacilli and also suppressing the growth of harmful pathogens e.g. E. coli
Availability: Items available for loan: UVAS Library [Call number: 2859-T] (1).
